Genomic structure and chromosome location of RPL27A/Rpl27a, the genes encoding human and mouse ribosomal protein L27A

1999 ◽  
Vol 85 (3-4) ◽  
pp. 248-251 ◽  
Author(s):  
J. Kusuda, ◽  
M. Hirai ◽  
R. Tanuma ◽  
M. Hirata ◽  
K. Hashimoto
Keyword(s):  
Ribosomal Protein ◽  
Genomic Structure ◽  
Chromosome Location ◽  
Genes Encoding ◽  
Human And Mouse

Genomic structure, chromosome location, and alternative splicing of the humanNKG2A gene

1996 ◽  
Vol 44 (4) ◽  
pp. 286-291 ◽  
Author(s):  
Béatrice Plougastel ◽  
Tania Jones ◽  
John Trowsdale
Keyword(s):  
Alternative Splicing ◽  
Genomic Structure ◽  
Chromosome Location

scholarly journals The sperm nucleus: chromatin, RNA, and the nuclear matrix

Reproduction ◽  
2011 ◽  
Vol 141 (1) ◽  
pp. 21-36 ◽  
Author(s):  
Graham D Johnson ◽  
Claudia Lalancette ◽  
Amelia K Linnemann ◽  
Frédéric Leduc ◽  
Guylain Boissonneault ◽  
...  
Keyword(s):  
Nuclear Matrix ◽  
Genomic Structure ◽  
Sperm Nucleus ◽  
Nuclear Volume ◽  
Clinical Markers ◽  
Paternal Genome ◽  
Epigenetic Effects ◽  
History Of ◽  
Low Levels ◽  
Human And Mouse

Within the sperm nucleus, the paternal genome remains functionally inert and protected following protamination. This is marked by a structural morphogenesis that is heralded by a striking reduction in nuclear volume. Despite these changes, both human and mouse spermatozoa maintain low levels of nucleosomes that appear non-randomly distributed throughout the genome. These regions may be necessary for organizing higher order genomic structure through interactions with the nuclear matrix. The promoters of this transcriptionally quiescent genome are differentially marked by modified histones that may poise downstream epigenetic effects. This notion is supported by increasing evidence that the embryo inherits these differing levels of chromatin organization. In concert with the suite of RNAs retained in the mature sperm, they may synergistically interact to direct early embryonic gene expression. Irrespective, these features reflect the transcriptional history of spermatogenic differentiation. As such, they may soon be utilized as clinical markers of male fertility. In this review, we explore and discuss how this may be orchestrated.

Human and Mouse ISLR (Immunoglobulin Superfamily Containing Leucine-Rich Repeat) Genes: Genomic Structure and Tissue Expression

Genomics ◽  
1999 ◽  
Vol 61 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Akemi Nagasawa ◽  
Jun Kudoh ◽  
Setsuko Noda ◽  
Yukihiko Mashima ◽  
Alan Wright ◽  
...  
Keyword(s):  
Genomic Structure ◽  
Tissue Expression ◽  
Immunoglobulin Superfamily ◽  
Leucine Rich Repeat ◽  
Human And Mouse

scholarly journals The 40S Ribosomal Protein S6 Response to Blue Light by Interaction with SjAUREO in Saccharina japonica

2019 ◽  
Vol 20 (10) ◽  
pp. 2414 ◽  
Author(s):  
Hexiang Luan ◽  
Jianting Yao ◽  
Zhihang Chen ◽  
Delin Duan
Keyword(s):  
Ribosomal Protein ◽  
Cdna Library ◽  
Blue Light ◽  
Cell Cycle Progression ◽  
Saccharina Japonica ◽  
Cdna Libraries ◽  
Cellular Division ◽  
Cycle Progression ◽  
Ribosomal Protein S6 ◽  
Genes Encoding

Blue light (BL) plays an important role in regulation of the growth and development of aquatic plants and land plants. Aureochrome (AUREO), the recent BL photoreceptor identified in photosynthetic stramenopile algae, is involved in the photomorphogenesis and early development of Saccharina japonica porophytes (kelp). However the factors that interact with the SjAUREO under BL conditions specifically are not clear. Here in our study, three high quality cDNA libraries with CFU over 5 × 106 and a recombination rate of 100% were constructed respectively through white light (WL), BL and darkness (DK) treatments to the juvenile sporophytes. Based on the constructed cDNA libraries, the interactors of SjAUREO were screened and analyzed. There are eighty-four genes encoding the sixteen predicted proteins from the BL cDNA library, sixty-eight genes encoding eighteen predicted proteins from the DK cDNA library, and seventy-four genes encoding nineteen proteins from the WL cDNA library. All the predicted proteins are presumed to interact with SjAUREO when co-expressed with SjAUREO seperately. The 40S ribosomal protein S6 (RPS6), which only exists in the BL treated cDNA library except for two other libraries, and which is essential for cell proliferation and is involved in cell cycle progression, was selected for detailed analysis. We showed that its transcription was up-regulated by BL, and was highly transcribed in the basal blade (meristem region) of juvenile sporophytes but less in the distal part. Taken together, our results indicated that RPS6 was highly involved in BL-mediated kelp cellular division and photomorphogenesis by interacting with SjAUREO.

Molecular and Functional Analyses of the Human and Mouse Genes Encoding AFG3L1, a Mitochondrial Metalloprotease Homologous to the Human Spastic Paraplegia Protein

Genomics ◽  
2001 ◽  
Vol 76 (1-3) ◽  
pp. 58-65 ◽  
Author(s):  
Gabriel Kremmidiotis ◽  
Alison E. Gardner ◽  
Chatri Settasatian ◽  
Anna Savoia ◽  
Grant R. Sutherland ◽  
...  
Keyword(s):  
Spastic Paraplegia ◽  
Genes Encoding ◽  
Functional Analyses ◽  
Human And Mouse

Genomic Structure and Chromosome Location of the Human mutT Homologue Gene MTH1 Encoding 8-Oxo-dGTPase for Prevention of A:T to C:G Transversion

Genomics ◽  
1994 ◽  
Vol 24 (3) ◽  
pp. 485-490 ◽  
Author(s):  
Masato Furuichi ◽  
Michihiro C. Yoshida ◽  
Hisanobu Oda ◽  
Tatsurou Tajiri ◽  
Yusaku Nakabeppu ◽  
...  
Keyword(s):  
Genomic Structure ◽  
Chromosome Location

Human and mouse RAD17 genes: identification, localization, genomic structure and histological expression pattern in normal testis and seminoma

1999 ◽  
Vol 105 (1-2) ◽  
pp. 17-27 ◽  
Author(s):  
F. von Deimling ◽  
J.M. Scharf ◽  
T. Liehr ◽  
M. Rothe ◽  
A.-R. Kelter ◽  
...  
Keyword(s):  
Expression Pattern ◽  
Genomic Structure ◽  
Normal Testis ◽  
Human And Mouse

scholarly journals A splice variant acquiring an extra transcript leader region decreases the translation of glutamine synthetase gene

2003 ◽  
Vol 374 (1) ◽  
pp. 175-184 ◽  
Author(s):  
Daesung SHIN ◽  
Sangjin PARK ◽  
Chankyu PARK
Keyword(s):  
Alternative Splicing ◽  
Glutamine Synthetase ◽  
Genomic Structure ◽  
Cell Types ◽  
Alternative Transcript ◽  
Exon Trapping ◽  
Exon 1 ◽  
Rapid Amplification ◽  
Altered Form ◽  
Human And Mouse

The expression of glutamine synthetase (GS), catalysing the ATP-dependent conversion of glutamate and ammonia into glutamine, is transcriptionally and post-transcriptionally regulated. The genomic structure of dog GS shown in the present study is basically similar to that of other mammals in that it is composed of seven exons and six introns. Using 5′-cRACE (where cRACE stands for circular rapid amplification of cDNA ends) and reverse transcriptase–PCR, we identified an additional exon (120 bp) in the first intron, designated in the present study as exon 1′. By means of alternative splicing, the GS gene produces an altered form of GS transcript with 5′-untranslated region (UTR) containing the exon 1′. This alternative transcript is abundantly expressed in brain, whereas it is found at lower levels in other tissues. In the human and mouse GS genes, extra exons are also found at the corresponding site of the intron 1 but with different sizes. An exon-trapping experiment for the GS gene in COS-7, Madin–Darby canine kidney and SK-N-SH cells revealed that the pattern of alternative splicing is variable in different cell types. The propensity of forming a secondary structure is predicted to be considerably higher in the presence of extra 5′-UTR, suggesting the possibility of a translational effect. To test this, we performed a reporter assay for fusions with different 5′-UTRs, demonstrating that the long form with extra 5′-UTR was translated 20- and 10-fold less than the short one in SK-N-SH and Neuro-2A cells respectively. Similarly, translations of human and mouse transcripts with extra 5′-UTRs were less efficient, showing 6–8-fold reductions in SK-N-SH cells. Furthermore, when we mutated an ATG sequence contained in the exon 1′, the suppression of translation was partially relieved, suggesting that the negative regulation by an extra 5′-UTR is, to some extent, due to an abortive translation from the upstream ATG.

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