Human and mouse RAD17 genes: identification, localization, genomic structure and histological expression pattern in normal testis and seminoma

1999 ◽  
Vol 105 (1-2) ◽  
pp. 17-27 ◽  
Author(s):  
F. von Deimling ◽  
J.M. Scharf ◽  
T. Liehr ◽  
M. Rothe ◽  
A.-R. Kelter ◽  
...  
Keyword(s):  
Expression Pattern ◽  
Genomic Structure ◽  
Normal Testis ◽  
Human And Mouse

Human and mouse RAD17 genes: identification, localization, genomic structure and histological expression pattern in normal testis and seminoma

1999 ◽  
Vol 105 (1-2) ◽  
pp. 17-27 ◽  
Author(s):  
F. von Deimling ◽  
J.M. Scharf ◽  
T. Liehr ◽  
M. Rothe ◽  
A.-R. Kelter ◽  
...  
Keyword(s):  
Expression Pattern ◽  
Genomic Structure ◽  
Normal Testis ◽  
Human And Mouse

scholarly journals The sperm nucleus: chromatin, RNA, and the nuclear matrix

Reproduction ◽  
2011 ◽  
Vol 141 (1) ◽  
pp. 21-36 ◽  
Author(s):  
Graham D Johnson ◽  
Claudia Lalancette ◽  
Amelia K Linnemann ◽  
Frédéric Leduc ◽  
Guylain Boissonneault ◽  
...  
Keyword(s):  
Nuclear Matrix ◽  
Genomic Structure ◽  
Sperm Nucleus ◽  
Nuclear Volume ◽  
Clinical Markers ◽  
Paternal Genome ◽  
Epigenetic Effects ◽  
History Of ◽  
Low Levels ◽  
Human And Mouse

Within the sperm nucleus, the paternal genome remains functionally inert and protected following protamination. This is marked by a structural morphogenesis that is heralded by a striking reduction in nuclear volume. Despite these changes, both human and mouse spermatozoa maintain low levels of nucleosomes that appear non-randomly distributed throughout the genome. These regions may be necessary for organizing higher order genomic structure through interactions with the nuclear matrix. The promoters of this transcriptionally quiescent genome are differentially marked by modified histones that may poise downstream epigenetic effects. This notion is supported by increasing evidence that the embryo inherits these differing levels of chromatin organization. In concert with the suite of RNAs retained in the mature sperm, they may synergistically interact to direct early embryonic gene expression. Irrespective, these features reflect the transcriptional history of spermatogenic differentiation. As such, they may soon be utilized as clinical markers of male fertility. In this review, we explore and discuss how this may be orchestrated.

Novel conserved elements upstream of theH19gene are transcribed and act as mesodermal enhancers

Development ◽  
2002 ◽  
Vol 129 (5) ◽  
pp. 1205-1213
Author(s):  
Robert A. Drewell ◽  
Katharine L. Arney ◽  
Takahiro Arima ◽  
Sheila C. Barton ◽  
James D. Brenton ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Pattern ◽  
Reporter Gene ◽  
Differentially Methylated Region ◽  
Genomic Sequencing ◽  
Strong Homology ◽  
Imprinting Control Region ◽  
Enhancer Function ◽  
Human And Mouse ◽  
Mouse Genomic

The reciprocally imprinted H19 and Igf2 genes form a co-ordinately regulated 130 kb unit in the mouse controlled by widely dispersed enhancers, epigenetically modified silencers and an imprinting control region (ICR). Comparative human and mouse genomic sequencing between H19 and Igf2 revealed two novel regions of strong homology upstream of the ICR termed H19 upstream conserved regions (HUCs). Mouse HUC1 and HUC2 act as potent enhancers capable of driving expression of an H19 reporter gene in a range of mesodermal tissues. Intriguingly, the HUC sequences are also transcribed bi-allelically in mouse and human, but their expression pattern in neural and endodermal tissues in day 13.5 embryos is distinct from their enhancer function. The location of the HUC mesodermal enhancers upstream of the ICR and H19, and their capacity for interaction with both H19 and Igf2 requires critical re-evaluation of the cis-regulation of imprinted gene expression of H19 and Igf2 in a range of mesodermal tissues. We propose that these novel sequences interact with the ICR at H19 and the epigenetically regulated silencer at differentially methylated region 1 (DMR1) of Igf2.

Human and Mouse ISLR (Immunoglobulin Superfamily Containing Leucine-Rich Repeat) Genes: Genomic Structure and Tissue Expression

Genomics ◽  
1999 ◽  
Vol 61 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Akemi Nagasawa ◽  
Jun Kudoh ◽  
Setsuko Noda ◽  
Yukihiko Mashima ◽  
Alan Wright ◽  
...  
Keyword(s):  
Genomic Structure ◽  
Tissue Expression ◽  
Immunoglobulin Superfamily ◽  
Leucine Rich Repeat ◽  
Human And Mouse

scholarly journals A splice variant acquiring an extra transcript leader region decreases the translation of glutamine synthetase gene

2003 ◽  
Vol 374 (1) ◽  
pp. 175-184 ◽  
Author(s):  
Daesung SHIN ◽  
Sangjin PARK ◽  
Chankyu PARK
Keyword(s):  
Alternative Splicing ◽  
Glutamine Synthetase ◽  
Genomic Structure ◽  
Cell Types ◽  
Alternative Transcript ◽  
Exon Trapping ◽  
Exon 1 ◽  
Rapid Amplification ◽  
Altered Form ◽  
Human And Mouse

The expression of glutamine synthetase (GS), catalysing the ATP-dependent conversion of glutamate and ammonia into glutamine, is transcriptionally and post-transcriptionally regulated. The genomic structure of dog GS shown in the present study is basically similar to that of other mammals in that it is composed of seven exons and six introns. Using 5′-cRACE (where cRACE stands for circular rapid amplification of cDNA ends) and reverse transcriptase–PCR, we identified an additional exon (120 bp) in the first intron, designated in the present study as exon 1′. By means of alternative splicing, the GS gene produces an altered form of GS transcript with 5′-untranslated region (UTR) containing the exon 1′. This alternative transcript is abundantly expressed in brain, whereas it is found at lower levels in other tissues. In the human and mouse GS genes, extra exons are also found at the corresponding site of the intron 1 but with different sizes. An exon-trapping experiment for the GS gene in COS-7, Madin–Darby canine kidney and SK-N-SH cells revealed that the pattern of alternative splicing is variable in different cell types. The propensity of forming a secondary structure is predicted to be considerably higher in the presence of extra 5′-UTR, suggesting the possibility of a translational effect. To test this, we performed a reporter assay for fusions with different 5′-UTRs, demonstrating that the long form with extra 5′-UTR was translated 20- and 10-fold less than the short one in SK-N-SH and Neuro-2A cells respectively. Similarly, translations of human and mouse transcripts with extra 5′-UTRs were less efficient, showing 6–8-fold reductions in SK-N-SH cells. Furthermore, when we mutated an ATG sequence contained in the exon 1′, the suppression of translation was partially relieved, suggesting that the negative regulation by an extra 5′-UTR is, to some extent, due to an abortive translation from the upstream ATG.

Human TIP49b/RUVBL2 gene: genomic structure, expression pattern, physical link to the human CGB/LHB gene cluster on chromosome 19q13.3

2000 ◽  
Vol 43 (2) ◽  
pp. 69-74 ◽  
Author(s):  
Béatrice Parfait ◽  
Yves Giovangrandi ◽  
Muriel Asheuer ◽  
Ingrid Laurendeau ◽  
Martine Olivi ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Expression Pattern ◽  
Genomic Structure
Sign in / Sign up

Export Citation Format

Share Document