scholarly journals Quantitative proteomic analysis of differentially expressed proteins in pancreatic cancer stem cells

2013 ◽  
Vol 21 (2) ◽  
pp. 145
Author(s):  
Jian-Xin Jiang ◽  
Shan Gao ◽  
Yao-Zhen Pan ◽  
Cheng-Yi Sun
Keyword(s):  
Pancreatic Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Proteomic Analysis ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Quantitative Proteomic Analysis ◽  
Pancreatic Cancer Stem Cells

Proteomic analysis of pancreatic cancer stem cells: Functional role of fatty acid synthesis and mevalonate pathways

2017 ◽  
Vol 150 ◽  
pp. 310-322 ◽  
Author(s):  
Jessica Brandi ◽  
Ilaria Dando ◽  
Elisa Dalla Pozza ◽  
Giulia Biondani ◽  
Rosalind Jenkins ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Stem Cells ◽  
Fatty Acid ◽  
Cancer Stem Cells ◽  
Fatty Acid Synthesis ◽  
Proteomic Analysis ◽  
Functional Role ◽  
Acid Synthesis ◽  
Pancreatic Cancer Stem Cells

scholarly journals Identification of differentially expressed microRNAs in pancreatic cancer stem cells

2012 ◽  
Vol 20 (34) ◽  
pp. 3354
Author(s):  
Jian-Xin Jiang ◽  
Shan Gao ◽  
Min Wang ◽  
Xu Li ◽  
Cheng-Yi Sun
Keyword(s):  
Pancreatic Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Differentially Expressed ◽  
Pancreatic Cancer Stem Cells

Differentially Expressed MicroRNAs in Pancreatic Cancer Stem Cells

Pancreas ◽  
2011 ◽  
Vol 40 (8) ◽  
pp. 1180-1187 ◽  
Author(s):  
Dawoon E. Jung ◽  
Jing Wen ◽  
Taeyun Oh ◽  
Si Young Song
Keyword(s):  
Pancreatic Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Differentially Expressed ◽  
Pancreatic Cancer Stem Cells

scholarly journals Label-free quantitative proteomic analysis of the inhibition effect of Lactobacillus rhamnosus GG on Escherichia coli biofilm formation in co-culture

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Huiyi Song ◽  
Ni Lou ◽  
Jianjun Liu ◽  
Hong Xiang ◽  
Dong Shang
Keyword(s):  
Escherichia Coli ◽  
Proteomic Analysis ◽  
Biofilm Formation ◽  
Lactobacillus Rhamnosus ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Label Free ◽  
Quantitative Proteomic Analysis ◽  
E Coli ◽  
Protein Ubiquitination

Abstract Background Escherichia coli (E. coli) is the principal pathogen that causes biofilm formation. Biofilms are associated with infectious diseases and antibiotic resistance. This study employed proteomic analysis to identify differentially expressed proteins after coculture of E. coli with Lactobacillus rhamnosus GG (LGG) microcapsules. Methods To explore the relevant protein abundance changes after E. coli and LGG coculture, label-free quantitative proteomic analysis and qRT-PCR were applied to E. coli and LGG microcapsule groups before and after coculture, respectively. Results The proteomic analysis characterised a total of 1655 proteins in E. coli K12MG1655 and 1431 proteins in the LGG. After coculture treatment, there were 262 differentially expressed proteins in E. coli and 291 in LGG. Gene ontology analysis showed that the differentially expressed proteins were mainly related to cellular metabolism, the stress response, transcription and the cell membrane. A protein interaction network and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analysis indicated that the differentiated proteins were mainly involved in the protein ubiquitination pathway and mitochondrial dysfunction. Conclusions These findings indicated that LGG microcapsules may inhibit E. coli biofilm formation by disrupting metabolic processes, particularly in relation to energy metabolism and stimulus responses, both of which are critical for the growth of LGG. Together, these findings increase our understanding of the interactions between bacteria under coculture conditions.

scholarly journals Integrated lipidomics and proteomics reveal cardiolipin alterations, upregulation of HADHA and long chain fatty acids in pancreatic cancer stem cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Claudia Di Carlo ◽  
Bebiana C. Sousa ◽  
Marcello Manfredi ◽  
Jessica Brandi ◽  
Elisa Dalla Pozza ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Stem Cells ◽  
Fatty Acid ◽  
Cancer Stem Cells ◽  
Acyl Chain ◽  
Fatty Acid Elongase ◽  
Acyl Chains ◽  
A Chain ◽  
Pancreatic Cancer Stem Cells ◽  
Long Chain

AbstractPancreatic cancer stem cells (PCSCs) play a key role in the aggressiveness of pancreatic ductal adenocarcinomas (PDAC); however, little is known about their signaling and metabolic pathways. Here we show that PCSCs have specific and common proteome and lipidome modulations. PCSCs displayed downregulation of lactate dehydrogenase A chain, and upregulation of trifunctional enzyme subunit alpha. The upregulated proteins of PCSCs are mainly involved in fatty acid (FA) elongation and biosynthesis of unsaturated FAs. Accordingly, lipidomics reveals an increase in long and very long-chain unsaturated FAs, which are products of fatty acid elongase-5 predicted as a key gene. Moreover, lipidomics showed the induction in PCSCs of molecular species of cardiolipin with mixed incorporation of 16:0, 18:1, and 18:2 acyl chains. Our data indicate a crucial role of FA elongation and alteration in cardiolipin acyl chain composition in PCSCs, representing attractive therapeutic targets in PDAC.

206 Identification and Whole Transcriptome Characterization of Pancreatic Cancer Stem Cells

2012 ◽  
Vol 142 (5) ◽  
pp. S-50-S-51
Author(s):  
Christina Vorvis ◽  
George A. Poultsides ◽  
Jeffrey A. Norton ◽  
Maria Hatziapostolou ◽  
Dimitrios Iliopoulos
Keyword(s):  
Pancreatic Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Whole Transcriptome ◽  
Pancreatic Cancer Stem Cells

Targeting pancreatic cancer stem cells for cancer therapy

2012 ◽  
Vol 1826 (2) ◽  
pp. 385-399 ◽  
Author(s):  
Jun Xia ◽  
Changjie Chen ◽  
Zhiwen Chen ◽  
Lucio Miele ◽  
Fazlul H. Sarkar ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Stem Cells ◽  
Cancer Therapy ◽  
Cancer Stem Cells ◽  
Pancreatic Cancer Stem Cells

scholarly journals Oncolytic Viruses Effectively Target and Kill Pancreatic Cancer Stem Cells

2011 ◽  
Vol 140 (5) ◽  
pp. S-1000-S-1001
Author(s):  
Joyce Wong ◽  
Allison Schulman ◽  
Arjun Mittra ◽  
Yuman Fong
Keyword(s):  
Pancreatic Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Oncolytic Viruses ◽  
Pancreatic Cancer Stem Cells
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