scholarly journals Influence of Genetic Strain Background on the Magnitude of Behavioral Recovery Observed in Weaver Mutant Mice Following Bilateral Intrastriatal Grafting of Mesencephalic Cell Suspensions

1992 ◽  
Vol 3 (4) ◽  
pp. 253-254
Author(s):  
L. C. Triarhou ◽  
J. Norton ◽  
B. Ghetti ◽  
J. N. Hingtgen
Keyword(s):  
Cell Suspensions ◽  
Mutant Mice ◽  
Behavioral Recovery ◽  
Genetic Strain ◽  
Strain Background

scholarly journals ThemecAHomologmecCConfers Resistance against β-Lactams in Staphylococcus aureus Irrespective of the Genetic Strain Background

2014 ◽  
Vol 58 (7) ◽  
pp. 3791-3798 ◽  
Author(s):  
Britta Ballhausen ◽  
André Kriegeskorte ◽  
Nina Schleimer ◽  
Georg Peters ◽  
Karsten Becker
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Regulatory System ◽  
Wild Type ◽  
Beta Lactam ◽  
Beta Lactams ◽  
Content Type ◽  
Genetic Strain ◽  
Mrsa Strains ◽  
Strain Background

ABSTRACTIn staphylococci, methicillin resistance is mediated bymecA-encoded penicillin-binding protein 2a (PBP2a), which has a low affinity for beta-lactams. Recently, a novel PBP2a homolog was described as being encoded bymecC, which shares only 70% similarity tomecA. To prove thatmecCis the genetic determinant that confers methicillin resistance inStaphylococcus aureus, amecCknockout strain was generated. TheS. aureusΔmecCstrain showed considerably reduced oxacillin and cefoxitin MICs (0.25 and 4 μg/ml, respectively) compared to those of the corresponding wild-type methicillin-resistantS. aureus(MRSA) strain (8 and 16 μg/ml, respectively). Complementing the mutant intranswith wild-typemecCrestored the resistance to oxacillin and cefoxitin. By expressingmecCandmecAin differentS. aureusclonal lineages, we found thatmecCmediates resistance irrespective of the genetic strain background, yielding oxacillin and cefoxitin MIC values comparable to those withmecA. In addition, we showed thatmecCexpression is inducible by oxacillin, which supports the assumption that a functional beta-lactam-dependent regulatory system is active in MRSA strains possessing staphylococcal cassette chromosomemec(SCCmec) type XI. In summary, we showed thatmecCis inducible by oxacillin and mediates beta-lactam resistance in SCCmectype XI-carrying strains as well as in differentS. aureusgenetic backgrounds. Furthermore, our results could explain the comparatively low MICs for clinicalmecC-harboringS. aureusisolates.

Serotonin fiber innervation of cerebellar cell suspensions intraparenchymally grafted to the cerebellum ofpcd mutant mice

1992 ◽  
Vol 17 (5) ◽  
pp. 475-482 ◽  
Author(s):  
Lazaros C. Triarhou ◽  
Walter C. Low ◽  
Bernardino Ghetti
Keyword(s):  
Cell Suspensions ◽  
Mutant Mice ◽  
Cerebellar Cell

Developmental expression of polypeptide PEP-19 in cerebellar cell suspensions transplanted into the cerebellum of pcd mutant mice

1989 ◽  
Vol 76 (3) ◽  
pp. 639-645 ◽  
Author(s):  
A. C. Chang ◽  
L. C. Triarhou ◽  
C. J. Alyea ◽  
W. C. Low ◽  
B. Ghetti
Keyword(s):  
Cell Suspensions ◽  
Mutant Mice ◽  
Developmental Expression ◽  
Cerebellar Cell

GENETIC STRAIN BACKGROUND INFLUENCES THE PHENOTYPIC EFFECTS OF THE MOUSE WEAVER GENE

1997 ◽  
Vol 56 (5) ◽  
pp. 587
Author(s):  
S. R. Dlouhy ◽  
T. Verinn ◽  
J. J. Wei ◽  
J. Richter ◽  
C. Stauss ◽  
...  
Keyword(s):  
Genetic Strain ◽  
Strain Background

A pre-embedding, protein a-gold immunolabelling technique for cytocentrifuged cell monolayers for lm and tem

1986 ◽  
Vol 44 ◽  
pp. 220-221
Author(s):  
K. Chien ◽  
I.P. Shintaku ◽  
A.F. Sassoon ◽  
R.L. Van de Velde ◽  
R. Heusser
Keyword(s):  
Cell Surface ◽  
Staining Method ◽  
Protein A ◽  
Surface Antigens ◽  
Cell Suspensions ◽  
Cellular Morphology ◽  
Cellular Phenotype ◽  
Cell Surface Antigens ◽  
Cell Monolayers ◽  
Diagnostic Histopathology

Identification of cellular phenotype by cell surface antigens in conjunction with ultrastructural analysis of cellular morphology can be a useful tool in the study of biologic processes as well as in diagnostic histopathology. In this abstract, we describe a simple pre-embedding, protein A-gold staining method which is designed for cell suspensions combining the handling convenience of slide-mounted cell monolayers and the ability to evaluate specimen staining specificity prior to EM embedding.

New insights into heparan sulphate biosynthesis from the study of mutant mice

2002 ◽  
Vol 69 ◽  
pp. 47-57 ◽  
Author(s):  
Catherine L. R. Merry ◽  
John T. Gallagher
Keyword(s):  
Growth Factors ◽  
Biosynthetic Pathway ◽  
Heparan Sulphate ◽  
Mutant Mice ◽  
Gene Products ◽  
Multiple Gene ◽  
Adhesion Proteins ◽  
Ligand Interactions

Heparan sulphate (HS) is an essential co-receptor for a number of growth factors, morphogens and adhesion proteins. The biosynthetic modifications involved in the generation of a mature HS chain may determine the strength and outcome of HS–ligand interactions. These modifications are catalysed by a complex family of enzymes, some of which occur as multiple gene products. Various mutant mice have now been generated, which lack the function of isolated components of the HS biosynthetic pathway. In this discussion, we outline the key findings of these studies, and use them to put into context our own work concerning the structure of the HS generated by the Hs2st-/- mice.

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