scholarly journals The Imbalance Between Metalloproteinases and Their Tissue Inhibitors Is Involved in the Pathogenesis of Dermatitis Herpetiformis

2005 ◽  
Vol 2005 (6) ◽  
pp. 373-379 ◽  
Author(s):  
Agnieszka Zebrowska ◽  
Joanna Narbutt ◽  
Anna Sysa-Jedrzejowska ◽  
Jozef Kobos ◽  
Elzbieta Waszczykowska
Keyword(s):  
Autoimmune Disease ◽  
Coeliac Disease ◽  
Healthy Subjects ◽  
Dermatitis Herpetiformis ◽  
Skin Lesions ◽  
Blister Fluid ◽  
Exact Role ◽  
Basal Keratinocytes ◽  
Intestinal Lesions ◽  
Skin Biopsies

Dermatitis herpetiformis (DH) is a subepidermal autoimmune disease characterized by skin and intestinal lesions consistent with coeliac disease. There are also some data that metalloproteinases (MMPs) are involved in the development of skin lesions in DH, however their exact role in this process is not fully understood. The aim of the study was to investigate whether MMPs and their inhibitors are involved in pathogenesis of DH. Skin biopsies were taken from 13 patients with active DH and from 10 healthy subjects. The localization and expression of MMPs and TIMPs were examined by immunohistochemistry. MMPs expression was detected in basal keratinocytes and in the whole epidermis in all of the DH subjects. Neutrophils in microabscesses and in blister fluid were also positive for MMPs. Expression of TIMPs was moderate or weak in all examined biopsies. Our results allow us to conclude that imbalance between these enzymes takes an important role in the pathogenesis of DH.

scholarly journals Mediators of Mast Cells in Bullous Pemphigoid and Dermatitis Herpetiformis

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Agnieszka Zebrowska ◽  
Malgorzata Wagrowska-Danilewicz ◽  
Marian Danilewicz ◽  
Olga Stasikowska-Kanicka ◽  
Lilianna Kulczycka-Siennicka ◽  
...  
Keyword(s):  
Mast Cells ◽  
Bullous Pemphigoid ◽  
Skin Diseases ◽  
Dermatitis Herpetiformis ◽  
Skin Lesions ◽  
Autoimmune Blistering Disease ◽  
Mmp9 Expression ◽  
Skin Biopsies ◽  
Cell Expression ◽  
Perilesional Skin

Bullous pemphigoid (BP) and dermatitis herpetiformis (DH) are skin diseases associated with inflammation. However, few findings exist concerning the role of mast cells in autoimmune blistering disease. Skin biopsies were taken from 27 BP and 14 DH patients, as well as 20 healthy individuals. Immunohistochemistry was used to identify the localization and mast cell expression of TNFα and MMP9 in skin lesions and perilesional skin. The serum concentrations of TNFα, MMP9, chymase, tryptase, PAF, and IL-4 were measured by immunoassay. TNFα and MMP9 expression in the epidermis and in inflammatory influxed cells in the dermis was detected in skin biopsies from patients. Although these mediators were found to be expressed in the perilesional skin of all patients, the level was much lower than that in lesional skin. Increased serum PAF levels were observed in BP patients. Mast cells may play an essential role in activating inflammation, which ultimately contributes to the tissue damage observed in BP and DH. Our findings suggest that differences in the pattern of cytokine expression directly contribute to variations in cellular infiltration in DH and BP.

scholarly journals Expression of Selected Integrins and Selectins in Bullous Pemphigoid

2007 ◽  
Vol 2007 ◽  
pp. 1-7 ◽  
Author(s):  
Agnieszka Żebrowska ◽  
Anna Sysa-Jędrzejowska ◽  
Małgorzata Wągrowska-Danilewicz ◽  
Ewa Joss-Wichman ◽  
Anna Erkiert-Polguj ◽  
...  
Keyword(s):  
Bullous Pemphigoid ◽  
Healthy Subjects ◽  
Skin Lesions ◽  
Control Group ◽  
Dermoepidermal Junction ◽  
Basal Keratinocytes ◽  
Membrane Components ◽  
Inflammatory Infiltrates ◽  
Basement Membrane Components

Blister development in bullous pemphigoid (BP) results from destruction of hemidesmosomes and basement membrane components within the dermoepidermal junction by autoantibodies. Adhesion molecules can take part in pathogenesis of this disease. The aim of the study was to determine the localization and expression of L- and E-selectins andβ1,β3, andβ4 integrins by immunohistochemistry in skin lesions of 21 patients with BP, compared with 10 healthy subjects. Expression of L and E selectins andβ1,β3 integrins was detected mainly in basal keratinocytes and in inflammatory infiltrates in the dermis, expression ofβ4 integrin was irregular and was detected mainly in dermal part of the blister, while in the control group only weak and single expression of the examined molecules was detected in basal keratinocytes and endothelium cells. The obtained results reveal the important role of selected selectins and integrins in development of skin lesions in BP.

scholarly journals IL-17 Expression in Dermatitis Herpetiformis and Bullous Pemphigoid

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Agnieszka Zebrowska ◽  
Malgorzata Wagrowska-Danilewicz ◽  
Marian Danilewicz ◽  
Olga Stasikowska-Kanicka ◽  
Anna Cynkier ◽  
...  
Keyword(s):  
Bullous Pemphigoid ◽  
Healthy Subjects ◽  
Inflammatory Process ◽  
Essential Role ◽  
Skin Diseases ◽  
Dermatitis Herpetiformis ◽  
Control Group ◽  
Bullous Diseases ◽  
Skin Biopsies ◽  
Perilesional Skin

Dermatitis herpetiformis (DH) and bullous pemphigoid (BP) are skin diseases associated with eosinophilic and neutrophilic infiltrations. Although cytokines are critical for the inflammatory process, there are single findings concerning concentration of IL-17 in bullous diseases. The goal of this study was to assess IL-17 expression in DH and BP patients. Skin biopsies were taken from 10 DH, 14 BP patients and from 10 healthy subjects. The localization and expression of IL-17 was studied by immunohistochemistry and the serum concentration was measured by immunoassays. Expression of IL-17 in the epidermis and in influxed cells in dermis was detected in skin biopsies. Expression of IL-17 was statistically higher in epidermis and infiltration cells in specimens from BP than from DH patients. Examined interleukin expression was detected in perilesional skin of all patients but it was much lower than in lesional skin. The expression of IL-17 was not observed in biopsies from healthy people. Serum level of IL-17 was statistically higher in BP and DH groups as compared to control group. Our results provide the evidence that IL-17 may play an essential role in activating and recruiting eosinophils and neutrophils, which ultimately contribute to the tissue damage in DH and BP.

scholarly journals Dermatitis Herpetiformis: From the Genetics to the Development of Skin Lesions

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Diletta Bonciani ◽  
Alice Verdelli ◽  
Veronica Bonciolini ◽  
Antonietta D'Errico ◽  
Emiliano Antiga ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Autoimmune Disease ◽  
Environmental Factors ◽  
Genetic Predisposition ◽  
Dermatitis Herpetiformis ◽  
Skin Lesions ◽  
Cutaneous Manifestation ◽  
Gluten Sensitivity

Dermatitis herpetiformis (DH) is a rare autoimmune disease linked to gluten sensitivity with a chronic-relapsing course. It is currently considered to be the specific cutaneous manifestation of celiac disease (CD). Both conditions are mediated by the IgA class of autoantibodies, and the diagnosis of DH is dependent on the detection of granular deposits of IgA in the skin. There is an underlying genetic predisposition to the development of DH, but environmental factors are also important. This paper describes these different factors and discusses the known mechanism that lead to the development of skin lesions.

scholarly journals The Expression of Selected Proapoptotic Molecules in Dermatitis Herpetiformis

2012 ◽  
Vol 2012 ◽  
pp. 1-6
Author(s):  
Zebrowska Agnieszka ◽  
Erkiert-Polguj Anna ◽  
Wagrowska-Danilewicz Malgorzata ◽  
Danilewicz Marian ◽  
Sysa-Jedrzejowska Anna ◽  
...  
Keyword(s):  
Fas Ligand ◽  
Dermatitis Herpetiformis ◽  
Single Cells ◽  
Skin Lesions ◽  
Control Group ◽  
Trail Expression ◽  
Skin Biopsies ◽  
And Control ◽  
Perilesional Skin

The role of the process of apoptosis is investigated in the pathogenesis of many autoimmune diseases; however at present, there is not much information about its role in dermatitis herpetiformis. Skin biopsies were taken from 18 DH patients and from 10 healthy subjects. The localization and expression of Bax, Fas, FasL, TRAIL, TRAIL-R in skin lesions, and perilesional skin were studied by immunohistochemistry. Expression of Bax, Fas, and Fas ligand was detected in the keratinocytes in skin biopsies from DH patients. Expression of TRAIL and TRAIL receptor was confirmed in epidermis, infiltration cells, and some fibroblasts. The expression of examined molecules in biopsies from healthy people was observed only in single cells. There were statistically significant differences between lesional, perilesional, and healthy skin of control group in Bax expression analysis and between lesional skin and control group in Fas, FasL, and TRAIL expression. There were statistically significant differences between control group and perilesional skin in Bax and FasL expression. Our results show that selected proapoptotic molecules may take part in pathogenesis of dermatitis herpetiformis, but the role of apoptosis in this process is not clear.

Photosensitization Induced by Heterophyllaea pustulata in Goats: Sequential Development of Skin Lesions

2021 ◽  
Vol 58 (3) ◽  
pp. 568-573
Author(s):  
Juan F. Micheloud ◽  
Lluís Luján ◽  
Luis A. Colque-Caro ◽  
Susana C. Núñez-Montoya ◽  
Claudio G. Barbeito ◽  
...  
Keyword(s):  
Cell Death ◽  
Oral Dose ◽  
Skin Lesions ◽  
Crust Formation ◽  
Cutaneous Lesions ◽  
Protein Ratio ◽  
Dermal Fibrosis ◽  
Sequential Development ◽  
Treatment Changes ◽  
Skin Biopsies

Five adult Saanen goats received a single oral dose of Heterophyllaea pustulata containing 42.25 μg/kg rubiadin (anthraquinone) and 3 adult goats were untreated controls. All goats were exposed to sunlight and sequential ear skin biopsies were collected before treatment and at 32 hours, 3 days, 8 days, and 15 days after treatment. Changes at 32 hours after dosing included epidermal spongiosis, single cell death and acantholysis, an increased BAX/BCL-2 protein ratio, and dermal edema. Lesions at day 3 included epidermal and adnexal necrosis, crust formation, and acanthosis. Acanthosis, hyperkeratosis, and dermal fibrosis and neovascularization were present at day 15. The pro-apoptotic (BAX)/anti-apoptotic (BCL-2) protein ratio increased at 32 hours, whereas epidermal and dermal PCNA immunolabeling increased between days 8 and 15 after treatment. The cutaneous lesions were consistent with sunlight-induced damage, and the occurrence in treated but not control goats indicates photosensitization.

Coeliac disease presenting atypically: A much wider spectrum

2021 ◽  
pp. 004947552199134
Author(s):  
Avinash Lomash ◽  
Abhinaya Venkatakrishnan ◽  
Meenakshi Bothra ◽  
Bhavna Dhingra ◽  
Praveen Kumar ◽  
...  
Keyword(s):  
Coeliac Disease ◽  
Gestational Age ◽  
Dermatitis Herpetiformis ◽  
Mode Of Delivery ◽  
Gastrointestinal Symptoms ◽  
Stunted Growth ◽  
Significant Difference ◽  
High Index Of Suspicion ◽  
Gestational Age At Birth ◽  
Age At Birth

Atypical coeliac disease in young children is frequently missed when it presents atypically as non-gastrointestinal presentations to different specialties. There was a greater delay (54 months) in establishing the diagnosis in those with atypical coeliac disease (p < 0.001). No difference was observed in the mode of delivery or duration of breast feeding, but significant difference was observed between gestational age at birth (p < 0.001). Most cases showed stunted growth and underweight. Irritability, anaemia, rickets, dermatitis herpetiformis, alopecia and intussusception were other common predictors of atypical coeliac disease. Because of a myriad spectrum of non-gastrointestinal symptoms, at any age with diverse presentation, a high index of suspicion is therefore required.

scholarly journals MCPIP1/Regnase-1 Expression in Keratinocytes of Patients with Hidradenitis Suppurativa: Preliminary Results

2021 ◽  
Vol 22 (14) ◽  
pp. 7241
Author(s):  
Piotr K. Krajewski ◽  
Weronika Szukała ◽  
Agata Lichawska-Cieślar ◽  
Łukasz Matusiak ◽  
Jolanta Jura ◽  
...  
Keyword(s):  
Western Blot ◽  
Mrna Expression ◽  
Hidradenitis Suppurativa ◽  
Skin Lesions ◽  
Healthy Controls ◽  
Lesional Skin ◽  
Chemotactic Protein ◽  
Healthy Control ◽  
Mrna And Protein Expression ◽  
Skin Biopsies

The pathogenesis of hidradenitis suppurativa (HS) is yet to be fully understood. However, inflammation is a key element in the development of skin lesions. The aim of this study was to evaluate the expression of monocyte chemotactic protein-1-induced protein-1 (MCPIP1) in the skin of patients suffering from HS. Skin biopsies of 15 patients with HS and 15 healthy controls were obtained and processed for immunohistochemistry, western blot, and real time PCR. The highest mean MCPIP1 mRNA expression was found in the inflammatory lesional skin of HS patients. It was significantly higher than MCPIP1 mRNA expression in the biopsies from both healthy controls and non-lesional skin of HS patients. Western blot analysis indicated that expression of MCPIP1 was elevated within both lesional and non-lesional skin compared to the healthy control. The increased MCPIP1 mRNA and protein expression level in HS lesions may indicate its possible role in the disease pathogenesis.

Histopathology of the soft parts in 50 patients with malignant external otitis

1989 ◽  
Vol 103 (4) ◽  
pp. 366-368 ◽  
Author(s):  
J. Bernheim ◽  
J. Sade
Keyword(s):  
Skin Lesions ◽  
External Otitis ◽  
Pathological Feature ◽  
External Ear ◽  
Ear Canal ◽  
Malignant External Otitis ◽  
External Ear Canal ◽  
External Canal ◽  
Skin Biopsies ◽  
Skin Coverage

AbstractReports concerning the lesions of the skin coverage of the external ear canal in malignant external otitis (M.E.O.) are very few. To evaluate this problem, we studied the skin lesions of 45 skin biopsies from 40 M.E.O. patients, 23 from regions covering the osseous part of the ear structure, seven from the cartilaginous part of the external canal and 15 from both parts. The epidermis was normal in nine, thickened in 16, with acanthotic thickening and pseudoepitheliomatous hyperplasia in 20. In the dermis the amount of collagen was normal, but it was infiltrated by the inflammatory process. Acute inflammation was observed in 16 biopsies, subacute in 23, chronic in six. A mixture of acute and chronic changes was present in 18 biopsies. No major abnormalities of the vasculature could be detected. The distinguishing pathological feature of M.E.O. concerns the typical topographic distribution of the inflammation in the osseous part of the external ear canal.

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