scholarly journals Expression of Selected Integrins and Selectins in Bullous Pemphigoid

2007 ◽  
Vol 2007 ◽  
pp. 1-7 ◽  
Author(s):  
Agnieszka Żebrowska ◽  
Anna Sysa-Jędrzejowska ◽  
Małgorzata Wągrowska-Danilewicz ◽  
Ewa Joss-Wichman ◽  
Anna Erkiert-Polguj ◽  
...  
Keyword(s):  
Bullous Pemphigoid ◽  
Healthy Subjects ◽  
Skin Lesions ◽  
Control Group ◽  
Dermoepidermal Junction ◽  
Basal Keratinocytes ◽  
Membrane Components ◽  
Inflammatory Infiltrates ◽  
Basement Membrane Components

Blister development in bullous pemphigoid (BP) results from destruction of hemidesmosomes and basement membrane components within the dermoepidermal junction by autoantibodies. Adhesion molecules can take part in pathogenesis of this disease. The aim of the study was to determine the localization and expression of L- and E-selectins andβ1,β3, andβ4 integrins by immunohistochemistry in skin lesions of 21 patients with BP, compared with 10 healthy subjects. Expression of L and E selectins andβ1,β3 integrins was detected mainly in basal keratinocytes and in inflammatory infiltrates in the dermis, expression ofβ4 integrin was irregular and was detected mainly in dermal part of the blister, while in the control group only weak and single expression of the examined molecules was detected in basal keratinocytes and endothelium cells. The obtained results reveal the important role of selected selectins and integrins in development of skin lesions in BP.

Predictive value of the neutrophil-to-lymphocyte ratio, monocyte-to-lymphocyte ratio, platelet-to-neutrophil ratio, and neutrophil-to-monocyte ratio in lupus nephritis

Lupus ◽  
2020 ◽  
Vol 29 (9) ◽  
pp. 1031-1039
Author(s):  
Peng Liu ◽  
Peiyuan Li ◽  
Zhong Peng ◽  
Yazhou Xiang ◽  
Chenqi Xia ◽  
...  
Keyword(s):  
Lupus Nephritis ◽  
Healthy Subjects ◽  
Area Under The Curve ◽  
Neutrophil To Lymphocyte Ratio ◽  
Control Group ◽  
Receiver Operating Characteristic Curves ◽  
Lymphocyte Ratio ◽  
And Control ◽  
First Time

Objective To evaluate the role of neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), monocyte-to-lymphocyte ratio (MLR), platelet-to-neutrophil ratio (PNR), platelet-to-monocyte ratio (PMR), and neutrophil-to-monocyte ratio (NMR) as predictors for lupus nephritis (LN) patients without infection or as biomarkers for distinguishing between infection or flare with LN patients. Methods LN patients were divided into three groups: LN without infection, LN with infection, and LN with flare. A total of 57 healthy subjects were enrolled as controls. The differentiation was analyzed between LN without infection and control group, and LN with infection and LN with flare. Correlations among variables were assessed in the LN group without infection. Receiver operating characteristic curves were constructed in two comparable groups. Results NLR, PLR, and MLR were increased significantly in the LN group without infection as compared with those in healthy controls. NLR (area under the curve (AUC): 0.75) and MLR (AUC: 0.79) were useful for distinguishing between LN patients without infection and healthy subjects. In differentiating LN patients without infection from the controls, optimal cutoffs of NLR and MLR were 3.43 (sensitivity: 45.6%, specificity: 96.5%, and overall accuracy: 68.8%) and 0.24 (sensitivity: 75.0%, specificity: 73.7%, and overall accuracy: 73.6%), respectively. In addition, NLR ( r = 0.322, p = 0.011) and PLR ( r = 0.283, p = 0.026) were positively correlated with CRP. Importantly, NLR and NMR were increased while PNR was decreased in the LN group with infection in comparison with those in the LN group with flare. NLR (AUC: 0.80), NMR (AUC: 0.78), and PNR (AUC: 0.74) were useful in differentiating LN patients with infection and flare, and their optimal cutoffs were 4.02 (sensitivity: 82.6%, specificity: 69.6%, and overall accuracy: 75.5%), 12.19 (sensitivity: 80.4%, specificity: 73.9%, and overall accuracy: 77.5%), and 28.26 (sensitivity: 65.2%, specificity: 76.8%, and overall accuracy: 71.6%), respectively. Conclusions We demonstrated, for the first time, that MLR or NMR had the best accuracy in differentiating LN patients without infection from healthy subjects, or differentiating infection from flare in LN patients, respectively. Our results implied that NLR, MLR, PNR, and NMR may be useful biomarkers in predicting LN.

scholarly journals Detection of Salivary Interleukin 2 and Interleukin 6 in Patients With Burning Mouth Syndrome

2006 ◽  
Vol 2006 ◽  
pp. 1-4 ◽  
Author(s):  
Daria Simcic ◽  
Sonja Pezelj-Ribaric ◽  
Renata Gržic ◽  
Jelena Horvat ◽  
Gordana Brumini ◽  
...  
Keyword(s):  
Healthy Subjects ◽  
Interleukin 2 ◽  
Severity Of Illness ◽  
Burning Mouth Syndrome ◽  
Control Group ◽  
Whole Saliva ◽  
Burning Mouth ◽  
Age Range ◽  
Healthy Participants

The etiology of BMS remains unknown. Role of various cytokines has been implicated in the development of BMS. The aim of this study was to evaluate levels of salivary IL-2 and IL-6 in patients with BMS, compared with age-matched healthy volunteers (control group). Whole saliva from 30 patients with BMS, age range 55–65, was tested for the presence of IL-6 and IL-2 by enzyme immunoassay. Control group consisted of 30 healthy participants, aged 55–65 years. Saliva IL-2 concentrations in BMS were significantly increased in patients compared to healthy subjects: mean34.1±9.7versus7.3±3.0pg/mL;P<.001. Patients with BMS had significantly higher concentrations of IL-6 compared to control: mean30.8±5.6versus5.2±2.8pg/mL;P<.001. In patients with BMS, IL-2 and IL-6 levels in saliva are elevated, correlating with the severity of illness.

scholarly journals The Role of Soluble L-Selectin with Polymorphism in Iraqi Arabs Patients with Diabetes Mellitus Type 2

2018 ◽  
Vol 9 (01) ◽  
Author(s):  
Asmaa M. Salih Almohaidi ◽  
Kebaa Ahmed Saeed
Keyword(s):  
Diabetes Mellitus ◽  
Serum Level ◽  
Healthy Subjects ◽  
Diabetes Mellitus Type 2 ◽  
The Body ◽  
Diabetes Mellitus Type ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group

Diabetes mellitus type 2 [DMT2] is a disturbance of metabolism and complex diseases influenced by environmental, genetic agents, and linked with inflammation, happens when the pancreas either does not use the insulin as it should or the body does not make enough insulin, lead to insulin resistance [IR] alongside with gradual loss of ß-cell secretory ability. The aim of this study was to investigate the role of soluble L-selectin (sL-selectin) in diabetes mellitus type 2 patients in Iraqi Arabs patient. Study includes seventy six Iraqi Arabs patients (male and female) having newly diagnosed type 2 diabetes mellitus (T2DM), with Fifty three Iraqi Arabs healthy subjects matched in age, sex and ethnic group. Patients and healthy subjects were genotyped, by PCR-RFLP analysis, and mesure serum level of L-selectin by enzyme-linked immunosorbent assay (sandwich ELISA) test include 65 patients and 23 controls. The statistical analysis of serum level of sL-selectin in study groups showed that the mean of sL-selectin level high significantly increased in patients group (10.708±1.1007) compared to control group (7.055±0.767) respectively. Thus, our results suggest soluble L-selectin play a role in the development of DMT2 in Iraqi Arabs patients. Present results showed that genotype PS associated with increase the susceptibility of DMT2.

IMPORTANT ROLE OF TRACE ELEMENTS (MG AND ZN) IN THE SERUM OF PATIENTS SUFFERING FROM NON-ALCOHOLIC LIVER DISEASE AND COMPARE THEIR LEVEL WITH HEALTHY SUBJECTS OF RAJASTHAN

2021 ◽  
pp. 29-31
Author(s):  
halini Vyas ◽  
RK Vyas ◽  
Hemlata Sharma
Keyword(s):  
Trace Elements ◽  
Liver Disease ◽  
Alcoholic Liver Disease ◽  
Healthy Subjects ◽  
Control Group ◽  
P Value ◽  
Wide Range ◽  
Range Of Functions ◽  
Serum Trace Elements

Liver is the largest major organ of our body. liver having a wide range of functions so, it is prone to many diseases which are very commonly seen in India. Any disturbance of liver function that causes illness is called liver disease. It is also known as hepatic disease. It is major leading cause of morbidity and mortality worldwide. Aim of this study was to nd out the role of Trace Elements (Mg & Zn) levels in diagnosis of Non-alcoholic liver disease. Material and methods: 200 subjects were selected out of them 100 were selected as study group (Non-alcoholic (NALD) and 100 were selected as control group and distributed according to their age and sex. Trace elements were estimated by Atomic adsorption spectrophotometer (AAS). Result: the serum trace elements level was decreased in non-alcoholic liver disease patients. P-value was found to be signicant when compared with healthy subjects. (P≤0.001). Conclusion: We came to the conclusion that serum level trace elements (Mg, Zn) activity looks specic and can be used for prognostic evolution of non-alcoholic liver disease.

scholarly journals Expression of the JAK/STAT Signaling Pathway in Bullous Pemphigoid and Dermatitis Herpetiformis

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
K. Juczynska ◽  
A. Wozniacka ◽  
E. Waszczykowska ◽  
M. Danilewicz ◽  
M. Wagrowska-Danilewicz ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Bullous Pemphigoid ◽  
Target Genes ◽  
Dermatitis Herpetiformis ◽  
Skin Lesions ◽  
Control Group ◽  
Cytokine Network ◽  
Activation Of Transcription ◽  
Autoimmune Bullous Diseases ◽  
Stat Signaling

A family of eleven proteins comprises the Janus kinases (JAK) and signal transducers and activators of transcription (STAT) signaling pathway, which enables transduction of signal from cytokine receptor to the nucleus and activation of transcription of target genes. Irregular functioning of the cascade may contribute to pathogenesis of autoimmune diseases; however, there are no reports concerning autoimmune bullous diseases yet to be published. The aim of this study was to evaluate the expression of proteins constituting the JAK/STAT signaling pathway in skin lesions and perilesional area in dermatitis herpetiformis (DH) and bullous pemphigoid (BP), as well as in the control group. Skin biopsies were collected from 21 DH patients, from 20 BP patients, and from 10 healthy volunteers. The localization and expression of selected STAT and JAK proteins were examined by immunohistochemistry and immunoblotting. We found significantly higher expression of JAK/STAT proteins in skin lesions in patients with BP and DH, in comparison to perilesional skin and the control group, which may be related to proinflammatory cytokine network and induction of inflammatory infiltrate in tissues. Our findings suggest that differences in the JAK and STAT expression may be related to distinct cytokines activating them and mediating neutrophilic and/or eosinophilic infiltrate.

scholarly journals The Effect of Different Attentional Focus Instructions during Finger Movement Tasks in Healthy Subjects: An Exploratory Study

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Giacomo Rossettini ◽  
Marco Testa ◽  
Marco Vicentini ◽  
Paolo Manganotti
Keyword(s):  
Healthy Subjects ◽  
Motor Performance ◽  
Attentional Focus ◽  
Focus Of Attention ◽  
Control Group ◽  
Finger Movement ◽  
External Focus ◽  
Internal Focus ◽  
And Control

External focus of attention (EFA) and internal focus of attention (IFA) represent commonly used strategies to instruct subjects during exercise. Several studies showed EFA to be more effective than IFA to improve motor performance and learning. To date the role of these strategies on motor performance during finger movement was less studied. The objective of the study was to investigate motor performance, patient’s preference induced by IFA and EFA, and the focus during control condition. Ten healthy right-handed participants performed a finger movement task in control, EFA, and IFA conditions (counterbalanced). Errors, patient’s preference, and type of attentional focus spontaneously adopted during the control condition were recorded. EFA determined less error (p<0.01) compared to control and IFA. Participants preferred EFA against IFA and control condition. In the control group 10% of subjects adopted a purely EFA, 70% of subjects adopted a purely IFA, and 20% of subjects adopted a mixture of the two foci. Our results confirm that EFA is more effective than IFA and control in finger movement task. Due its clinical relevance, the interaction between attention and finger movement should be further investigated.

scholarly journals The Antioxidant Profiles, Lysosomal and Membrane Enzymes Activity in Patients with Acute Pancreatitis

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Halina Milnerowicz ◽  
Radosław Bukowski ◽  
Monika Jabłonowska ◽  
Milena Ściskalska ◽  
Stanisław Milnerowicz
Keyword(s):  
Oxidative Stress ◽  
Acute Pancreatitis ◽  
Healthy Subjects ◽  
Control Group ◽  
Activity Levels ◽  
First Line ◽  
Course Of Disease ◽  
Antioxidative Status ◽  
Gpx Activity

Oxidative stress and inflammatory mediators, such as IL-6, play an important role in the pathophysiology of acute pancreatitis. The study was aimed to assess the degree of the pro/antioxidative imbalance and estimate which antioxidant plays a role in the maintenance of pro/antioxidative balance during acute pancreatitis. The study was investigated in the blood of 32 patients with acute pancreatitis and 37 healthy subjects. IL-6 concentration as early marker of inflammation was determinated. The intensity of oxidative stress was assessed by TBARS concentration. To investigate antioxidative status, the GPx and Cu/Zn SOD activities and the levels of GSH, MT, SH groups, and TRAP were measured. The concentrations of Cu and Zn as ions participating in the maintenance of antioxidant enzymes stability and playing a role in the course of disease were determinated. The activities of GGT, AAP, NAG, andβ-GD as markers of tissue damage were also measured. An increase in IL-6 concentration, which correlated with Ranson criteria, and an increase in GPx activity, levels of MT, TBARS, or GGT, and NAG activities in patients group compared to healthy subjects were demonstrated. A decrease in GSH level in patients group compared to control group was noted. The studies suggest that GPx/GSH and MT play the role of the first line of defence against oxidative stress and pro/antioxidant imbalance in the course of acute pancreatitis.

scholarly journals Colonic inflammation induces changes in glucose levels through modulation of incretin system

2021 ◽  
Author(s):  
Hubert Zatorski ◽  
Maciej Salaga ◽  
Marta Zielińska ◽  
Anna Mokrowiecka ◽  
Damian Jacenik ◽  
...  
Keyword(s):  
Glucose Level ◽  
Healthy Subjects ◽  
Fasting Glucose ◽  
Control Group ◽  
Trinitrobenzenesulfonic Acid ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Dpp Iv ◽  
Prohormone Convertase 1

Abstract Background The role of the incretin hormone, glucagon-like peptide (GLP-1), in Crohn’s disease (CD), is still poorly understood. The aim of this study was to investigate whether colitis is associated with changes in blood glucose levels and the possible involvement of the incretin system as an underlaying factor. Methods We used a murine model of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS). Macroscopic and microscopic score and expression of inflammatory cytokines were measured. The effect of colitis on glucose level was studied by measurement of fasting glucose and GLP-1, dipeptidyl peptidase IV (DPP IV) levels, prohormone convertase 1/3 (PC 1/3) and GLP-1 receptor (GLP-1R) expression in mice. We also measured the level of GLP-1, DPP IV and expression of glucagon (GCG) and PC 1/3 mRNA in serum and colon samples from healthy controls and CD patients. Results Fasting glucose levels were increased in animals with colitis compared to controls. GLP-1 was decreased in both serum and colon of mice with colitis in comparison to the control group. DPP IV levels were significantly increased in serum, but not in the colon of mice with colitis as compared to healthy animals. Furthermore, PC 1/3 and GLP-1R expression levels were increased in mice with colitis as compared to controls. In humans, no differences were observed in fasting glucose level between healthy subjects and CD patients. GLP-1 levels were significantly decreased in the serum. Interestingly, GLP-1 level was significantly increased in colon samples of CD patients compared to healthy subjects. No significant differences in DPP IV levels in serum and colon samples were observed between groups. Conclusions Changes in the incretin system during colitis seem to contribute to the impaired glucose levels. Differences in incretin levels seem to be modulated by degrading enzyme DPP-IV and PC 1/3. Obtained results suggest that the incretin system may become a novel therapeutic approach in the treatment of CD.

scholarly journals Expression of MMP-2 and MMP-13 in Bullous Pemphigoid

2019 ◽  
Vol 4 (1) ◽  
Author(s):  
Haixiang Zhang ◽  
Xiaoxu Shi ◽  
Lanying Qin ◽  
Zishen Zhao ◽  
Guojun Fu ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Bullous Pemphigoid ◽  
Normal Control ◽  
Normal Skin ◽  
Skin Lesions ◽  
Normal Control Group ◽  
High Expression ◽  
Matrix Metalloproteinase 2 ◽  
Control Group ◽  
Case Group

Objective: To investigate the expression and significance of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-13 (MMP-13) in bullous pemphigoid (BP) skin lesions. Methods: Immunohistochemical SP method was used to detect the expression of MMP-2 and MMP-13 in 32 BP skin lesions, and compared with 15 normal skin tissues. Results: The expression of MMP-2 in the case group was significantly increased (38.56 ± 10.06) compared to the normal control group (21.20 ± 5.98); the expression of MMP-13 in the case group was significantly augmented (18.62 ± 5.90) compared to the normal control group (11.47 ± 8.484). The expressions of MMP-2 and MMP-13 in the skin lesions of patients with bullous pemphigoid were statistically different from those of normal people (both P < 0.05). Compared with the expression of MMP-2 and MMP-13 in bullous pemphigoid, the expression of MMP-2 and MMP-13 was moderately correlated (correlation coefficient was 0.523). Conclusion: The expression of MMP-2 and MMP-13 is significantly increased in bullous pemphigoid skin lesions, suggesting that they may play an important role in the pathogenesis of BP. There is a certain correlation between the expression of MMP-2 and MMP-13, suggesting that the high expression of MMP-13 may play a role in the mechanism that further leads to the high expression of MMP-2.

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