scholarly journals Effects of Pulsed Radiofrequency on Nerve Repair and Expressions of GFAP and GDNF in Rats with Neuropathic Pain

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Qing Zhu ◽  
Yi Yan ◽  
Daying Zhang ◽  
Qingtian Luo ◽  
Cuihua Jiang
Keyword(s):  
Neuropathic Pain ◽  
Sciatic Nerve ◽  
Western Blot ◽  
Pain Sensitivity ◽  
Treatment Time ◽  
Nerve Repair ◽  
Nerve Fibers ◽  
Nerve Tissue ◽  
Control Group ◽  
Pulsed Radiofrequency

Objective. To study the effect of pulsed radio frequency (PRF) on nerve repair and the expression of GFAP and GDNF in rats with neuropathic pain. Methods. Thirty SPF healthy SD rats were randomly divided into control group (Group C), PSNL group (partial ligation of sciatic nerve) + sham group (Group PS), and PSNL group (partial ligation of sciatic nerve) + PRF group (Group PR), with 10 rats in each group. In group C, the right sciatic nerve was exposed without ligation. In the PS group, the model of neuropathic pain was established by partial ligation of sciatic nerve. The mice in the PR group were treated with PRF after establishing the neuropathic pain model. The general behavior of rats during the treatment was observed. The mechanical and thermal hyperalgesia were measured before operation and 1, 3, 7, and 14 days after operation. The content of inflammatory factors in nerve tissue was detected by ELISA. The pathological condition of nerve tissue was observed by HE. The gene and protein changes of GFAP and GDNF in nerve tissue were determined by QRT PCR and Western blot. Results. Rats in the control group were free to move and in good condition. In the PS group, there were different degrees of claudication, weakness of the lower limbs, lateral toe valgus, nerve injury, and other behavioral changes. After the pulsed radiofrequency in the PR group, the above symptoms decreased gradually with the prolongation of the treatment time. The mechanical pain sensitivity and thermal allodynia of the PS group were reduced after the operation. The mechanical pain sensitivity and thermal pain sensitivity of the PR group gradually increased with the prolongation of the treatment time, and the 14 days were basically close to the control group. The levels of TNF-α and IL-6 in ELISA were significantly higher in the PS group than in the control group, and the content in the PR group was gradually reduced, which was close to the control group. HE staining showed that the sciatic nerve fibers disappeared, and the formation of nerve cavities was obvious in the 14-day PS group. The nerve fibers were found in the sciatic tissue of the PR group, and there was no obvious hemorrhagic edema and cell deformation. The expression of GFAP mRNA in the PS group was significantly higher than that in the control group and the PR group ( p < 0.05 ), and the expression of GDNF was opposite ( p < 0.05 ). The results of western blot showed that the expression of GFAP protein in the 14-day PS group was significantly higher than that in the control group. The expression of the PR group decreased compared with the control group, and the expression of GDNF was opposite ( p < 0.05 ). Conclusion. Pulsed radiofrequency ablation can promote neurological repair, promote GDNF, and reduce the expression of GFAP in rats with neuropathic pain.

scholarly journals Evaluation of the Effect of Duration on the Efficacy of Pulsed Radiofrequency in an Animal Model of Neuropathic Pain

2018 ◽  
Vol 1 (21;1) ◽  
pp. 191-198
Author(s):  
Khaled I. Khalil
Keyword(s):  
Animal Model ◽  
Neuropathic Pain ◽  
Sciatic Nerve ◽  
Tissue Injury ◽  
Statistical Significance ◽  
Analgesic Efficacy ◽  
Control Group ◽  
Pulsed Radiofrequency ◽  
Tnf Α ◽  
Histopathological Evaluation

Background: Pulsed radiofrequency (PRF) is increasingly used in clinical practice, especially in neuropathic pain disorders. Although PRF is not new to clinical use, there are significant gaps in knowledge regarding its effectiveness. Objectives: The current study was conducted to evaluate the effect of duration of application of PRF on its analgesic efficacy in improvement of neuropathic pain. Study Design: A randomized experimental trial. Setting: An animal research facility at the College of Veterinary Medicine at Mansoura University in Egypt. Methods: Chronic constriction of the sciatic nerve of 36 male Sprague-Dawley rats was performed to induce neuropathic pain. The rats were divided into 6 groups (6 rats each) in which PRF was applied for 2, 4, 6, or 8 minutes or not at all. In one group, RF cannula was applied without performing PRF intervention. The pain was assessed through observation of resting paw posture (RPP) at 3, 10, and 21 days. Nerve damage was assessed by histopathological evaluation of the sciatic nerve. Immunohistochemical localization of proinflammatory cytokines (interleukin 6 [IL-6] and tumor necrosis factor alpha [TNF-α]) was also done. Results: RPP was improved in rats treated with PRF. This improvement was significant only in rats treated for 8 minutes. Increased duration for PRF application was associated with a significant decrease in IL-6 and TNF-α contents in all groups when compared with the control group. Histopathological evaluation of the constricted sciatic nerve revealed no statistical significance among the different study groups. Limitations: The study was limited by the lack of measurement of other inflammatory markers that may help elucidate other relevant mechanisms. Conclusions: Increased duration of PRF application resulted in better analgesic efficacy without any increase in tissue injury in an animal model of neuropathic pain. This effect may be attributed to decreased production of pro-inflammatory cytokines. Key words: Pulsed radiofrequency, analgesic, rats, sciatic nerve, duration, neuropathic pain

EFFICACY OF P-COUMARIC ACID IN CHRONIC CONSTRICTION INJURY INDUCED NEUROPATHIC PAIN IN RATS

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (09) ◽  
pp. 52-58
Author(s):  
Akash Bharti ◽  
Jaspreet Kaur ◽  
Amit Kumar ◽  
Simranjit Singh ◽  
Deepak Kumar ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Sciatic Nerve ◽  
Pain Sensitivity ◽  
Chronic Constriction Injury ◽  
Tissue Sample ◽  
Research Work ◽  
Nerve Tissue ◽  
Coumaric Acid ◽  
Biochemical Tests ◽  
Standard Drug

The present research work has been designed to evaluate the effect of p-coumaric acid in chronic constriction injury (CCI) of sciatic nerve induced neuropathic pain in rats. In addition, biochemical tests such as thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH) and total protein were performed in sciatic nerve tissue sample. The neuropathic pain has been effi ciently and successfully induced in rat by the performance of CCI. The battery of behavioural test showed the development of neuropathic pain as an index of rising the paw and tail thermal and mechanical pain sensitivity. The treatment of p-coumaric acid at dose 50 and 100 mg kg-1 , p.o. for 15 consecutive days have been shown to produce signifi cant ameliorative effect on CCI of sciatic nerve induced neuropathic pain sensitivity. In addition, CCI of sciatic nerve also induces the oxidative stress in nervous system by rising TBARS, decrease GSH and proteins levels in sciatic nerve tissue and these effects are reversed via administration of p-coumaric acid and statistically equivalent to standard drug. Hence, it may be concluded that, p-coumaric acid can be useful in the management of neuropathic pain symptoms.

scholarly journals Conjugated linoleic acid attenuates neuropathic pain induced by sciatic nerve in mice

2021 ◽  
Vol 18 (9) ◽  
pp. 1895-1901
Author(s):  
Qingbin Shi ◽  
Xiuying Cai ◽  
Changchun Li ◽  
Zhen Wang ◽  
Xingle Lv
Keyword(s):  
Neuropathic Pain ◽  
Linoleic Acid ◽  
Sciatic Nerve ◽  
Conjugated Linoleic Acid ◽  
Nerve Fibers ◽  
Vehicle Group ◽  
Control Group ◽  
Sham Operation ◽  
Behavioral Tests ◽  
Myelin Thickness

Purpose: Conjugated linoleic acid (CLA) has been suggested to be necessary for human health, but there is limited research regarding its effect on neuropathic pain (NP). Here, we aim to investigate the potential effect of CLA administration on NP development and nerve recovery. Methods: Forty mice were divided into four equal groups randomly. The mice in control group underwent a sham operation to achieve a unilateral sciatic nerve cut. Other groups were subjected to partial sciatic nerve ligation (PSNL) surgery followed by 4 weeks of CLA treatment. Behavioral tests were performed shortly before mice were sacrificed. Blood, sciatic nerve and spinal cord tissues were collected after sacrifice. Electron microscopy was performed to determine myelin thickness and calculate myelin thickness/axon diameter ratio. Results: Mice that received daily oral CLA treatment for 4 weeks after PSNL surgery showed less mechanical and thermal allodynia than mice in PSNL surgery alone group. Behavioral tests showed that CLA treatment was associated with marked increases in both nerve conduction velocity (NCV) and force of gastrocnemius contraction. In addition, CLA reduced the levels of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α), sciatic myeloperoxidase (MPO) activity, and activating transcription factor-3 (ATF-3) expression. CLA also restored mitochondrial manganese superoxide dismutase (MnSOD) activity which was decreased in the sciatic nerves and spinal cords of the PSNL surgery group. Regeneration of myelins and axons in nerve fibers in CLA group was faster and more complete than that in the vehicle group. Conclusion: The current study demonstrates that CLA effectively attenuates NP and significantly inhibits neuro-inflammation and oxidative stress. This treatment improves sciatic nerve form and function after injury, suggesting that it can attenuate NP.

scholarly journals Ameliorative potential of Vernonia cinerea on chronic constriction injury of sciatic nerve induced neuropathic pain in rats

2014 ◽  
Vol 86 (3) ◽  
pp. 1435-1450 ◽  
Author(s):  
VENKATA R.K. THIAGARAJAN ◽  
PALANICHAMY SHANMUGAM ◽  
UMA M. KRISHNAN ◽  
ARUNACHALAM MUTHURAMAN
Keyword(s):  
Neuropathic Pain ◽  
Sciatic Nerve ◽  
Chronic Constriction Injury ◽  
Ethanolic Extract ◽  
Thiobarbituric Acid ◽  
Nerve Tissue ◽  
Control Group ◽  
Dependent Manner ◽  
Pretreated Group ◽  
Vernonia Cinerea

The aim of the present study is to investigate the ameliorative potential of ethanolic extract of whole plant of Vernonia cinerea in the chronic constriction injury (CCI) of sciatic nerve induced neuropathic pain in rats. Behavioral parameters such as a hot plate, acetone drop, paw pressure, Von Frey hair and tail immersion tests were performed to assess the degree of thermal, chemical and mechanical hyperalgesia and allodynia. Biochemical changes in sciatic nerve tissue were ruled out by estimating thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH) and total calcium levels. Ethanolic extract of Vernonia cinerea and pregabalin were administered for 14 consecutive days starting from the day of surgery. CCI of sciatic nerve has been shown to induce significant changes in behavioral, biochemical and histopathological assessments when compared to the sham control group. Vernonia cinerea attenuated in a dose dependent manner the above pathological changes induced by CCI of the sciatic nerve, which is similar to attenuation of the pregabalin pretreated group. The ameliorating effect of ethanolic extract of Vernonia cinerea against CCI of sciatic nerve induced neuropathic pain may be due to the presence of flavonoids and this effect is attributed to anti-oxidative, neuroprotective and calcium channel modulator actions of these compounds.

scholarly journals Preemptive analgesic effect of lidocaine in a chronic neuropathic pain model

2009 ◽  
Vol 67 (4) ◽  
pp. 1088-1092 ◽  
Author(s):  
Leonardo M. Batista ◽  
Igor M. Batista ◽  
João P. Almeida ◽  
Carlos H. Carvalho ◽  
Samuel B. de Castro-Costa ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Sciatic Nerve ◽  
Control Group ◽  
Thermal Stimulus ◽  
Lidocaine Group ◽  
Local Infiltration ◽  
Neuropathic Pain Model ◽  
Group 3 ◽  
Group 2 ◽  
The Right

Preemptive analgesia inhibits the progression of pain caused by surgical lesions. To analyze the effect of lidocaine on postoperative pain relief, we performed compression of the right sciatic nerve in Wistar rats and observed the differences on behavior between the group that received lidocaine and the group that was not treated with the local anesthetics pre-operatively. Group 1 was not operated (control); group 2 underwent the sciatic nerve ligature without lidocaine; group 3, underwent surgery with previous local infiltration of lidocaine. Group 2 showed significantly longer scratching times with a peak on day 14 post-operative (p=0.0005) and reduction in the latency to both noxious (p=0.003) and non-noxious (p=0.004) thermal stimulus. Group 3 presented significantly shorter scratching times (p=0.004) and longer latency times when compared to Group 2. Preemptive use of lidocaine 2% can potentially reduce the postoperative neuropathic pain associated with sciatic nerve compression.

scholarly journals Internal-Specific Morphological Analysis of Sciatic Nerve Fibers in a Radiofrequency-Induced Animal Neuropathic Pain Model

PLoS ONE ◽  
2013 ◽  
Vol 8 (9) ◽  
pp. e73913 ◽  
Author(s):  
Samjin Choi ◽  
Hyuk Jai Choi ◽  
Youjin Cheong ◽  
Young-Jin Lim ◽  
Hun-Kuk Park
Keyword(s):  
Neuropathic Pain ◽  
Sciatic Nerve ◽  
Morphological Analysis ◽  
Nerve Fibers ◽  
Pain Model ◽  
Neuropathic Pain Model

Timing of Applying Electrical Stimulation Is an Important Factor Deciding the Success Rate and Maturity of Regenerating Rat Sciatic Nerves

2010 ◽  
Vol 24 (8) ◽  
pp. 730-735 ◽  
Author(s):  
Chia-Chou Yeh ◽  
Yu-Ching Lin ◽  
Fuu-Jen Tsai ◽  
Chih-Yang Huang ◽  
Chun-Hsu Yao ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Repair ◽  
Control Group ◽  
Adult Rats ◽  
Short Delay ◽  
Mean Values ◽  
Compound Muscle Action Potentials ◽  
Group B

Background. The timing of electrical stimulation (ES) after peripheral nerve transection may enhance axonal regeneration and functional recovery. Objective. The authors examined whether percutaneous ES at 1 mA and 2 Hz affects regeneration between the proximal and distal nerve stumps. Methods. Four groups of adult rats were subjected to sciatic nerve section followed by repair using silicone rubber conduits across a 10-mm gap. All groups received ES for 15 minutes every other day for 2 weeks. Stimulation was initiated on day 1 following the nerve repair for group A, day 8 for group B, and day 15 for group C. The control group D received no ES. Results. At 6 weeks after surgery in groups B and C, histological evaluations showed a significantly higher number of regenerated myelinated fibers in the sciatic nerve, and the electrophysiological results showed higher levels of reinnervation with relatively larger mean values of amplitudes, durations, and areas of compound muscle action potentials compared with A and D. Conclusion. A short delay in the onset of ES may improve the recovery of a severe peripheral nerve injury, which should be considered as a way of augmenting rehabilitative approaches.

scholarly journals Nociceptive and Neuronal Evaluation of the Sciatic Nerve of Wistar Rats Subjected to Compression Injury and Treated with Resistive Exercise

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Juliana Sobral Antunes ◽  
Keli Lovison ◽  
Jhenifer Karvat ◽  
Ana Luiza Peretti ◽  
Lizyana Vieira ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Physical Exercise ◽  
Resistance Exercise ◽  
Wistar Rats ◽  
Treated Group ◽  
Nerve Fibers ◽  
Lesion Group ◽  
Control Group ◽  
Compression Injury ◽  
Inflammatory Infiltration

Background. To investigate the climb stairs resistance exercise on nociception and axonal regeneration in the sciatic nerve of rats.Methods. 24 Wistar rats were divided: control group (CG—no injury), exercise group (EG—no injury with physical exercise), lesion group (LG—injury, but without exercise), and treated group (LEG—injury and physical exercise). LG and LEG were subjected to sciatic nerve compression with hemostat. From the 3rd day after injury began treatment with exercise, and after 22 days occurs the removal of a nerve fragment for morphological analysis.Results. Regarding allodynia, CG obtained values less than EG(p=0.012)and larger than LG and LEG(p<0.001). Histological results showed that CG and EG had normal appearance, as LG and LEG showed up with large amounts of inflammatory infiltration, degeneration and disruption of nerve fibers, and reduction of the myelin sheath; however LEG presented some regenerated fibers. From the morphometric data there were significant differences, for nerve fiber diameter, comparing CG with LG and LEG and comparing axon diameter and the thickness of the myelin of the CG to others.Conclusion. Climb stairs resistance exercise was not effective to speed up the regenerative process of axons.

scholarly journals Electrospun polycaprolactone (PCL)-amnion nanofibrous membrane prevents adhesions and promotes nerve repair in a rat model of sciatic nerve compression

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0244301
Author(s):  
Ruiyi Dong ◽  
Chunjie Liu ◽  
Siyu Tian ◽  
Jiangbo Bai ◽  
Kunlun Yu ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Nerve Regeneration ◽  
Rat Model ◽  
Nerve Repair ◽  
Adhesion Formation ◽  
Nerve Compression ◽  
Nanofibrous Membrane ◽  
Control Group ◽  
Chitosan Hydrogel

Adhesion and scarring after neural surgery are detrimental to nerve regeneration and functional recovery. Amniotic membranes have been used in tissue repair due to their immunogenicity and richness in cytokines. In this study, an electrospun polycaprolactone (PCL)-amnion nanofibrous membrane was prepared for the treatment of sciatic nerve compression in a rat model. The effects of the PCL-amnion nanofibrous membrane on the prevention of adhesion formation and nerve regeneration were evaluated using electrophysiology and histological analyses. Compared with the medical chitosan hydrogel dressing, the PCL-amnion nanofibrous membrane significantly reduced peripheral nerve adhesion and promoted the rapid recovery of nerve conduction. Moreover, the immunohistochemical analysis identified more Schwann cells and less pro-inflammatory M1 macrophages in the PCL-amnion group. Western blot and RT-PCR results showed that the expression levels of type-Ⅰ and Ⅲ collagen in the PCL-treated rats were half of those in the control group after 12 weeks, while the expression level of nerve growth factor was approximately 3.5 times that found in the rats treated with medical chitosan hydrogel. In summary, electrospun PCL-amnion nanofibrous membranes can effectively reduce adhesion after neural surgery and promote nerve repair and regeneration. The long-term retention in vivo and sustained release of cytokines make PCL-amnion a promising biomaterial for clinical application.

scholarly journals Creation of an Ideal Microenvironment by Reverse Endto-side Coaptation for the Evaluation of Tissue Neurotropism: An Experimental Model

2021 ◽  
Vol 1 (4) ◽  
Author(s):  
Balik O
Keyword(s):  
Experimental Model ◽  
Peroneal Nerve ◽  
Nerve Fibers ◽  
Nerve Graft ◽  
The Other ◽  
Nerve Tissue ◽  
Control Group ◽  
Group 4 ◽  
Group 3 ◽  
Group 2

Most studies agree on the demonstration of the existence of nerve tissue specificity and that if a non-nervous target (e.g. a tendon) were sutured to one of the distal limbs of a Y-shaped nerve guide, independent of the type of guide, all nerve fibers would regenerate toward the nerve tissue. Recent studies suggest that optimum nerve regeneration may arise from the tissue environment in general. We developed an experimental model in order to eliminate all the microenvironment problems that we experienced in previous studies, and to be able to evaluate the neurotropic affinity of various tissues. Group 1 (n:8): The control group. The sciatic nerve was only explored, but certainly not touched. Group 2 (n:8): Both lower ends of the peroneal nerve graft, prepared by reverse end-to-side coaptation in the shape of a horseshoe, were coapted to tibial and peroneal nerves distally by end-to-end coaptation. Group 3 (n:8): As one end of the peroneal nerve graft, prepared by reverse end-to-side coaptation in the shape of a horseshoe, was coapted to the peroneal nerve distally, the other end was sutured directly by opening the fascia of the gastrocnemius muscle. Group 4 (n:8): As one end of the peroneal nerve graft, prepared by reverse end-to-side coaptation in the shape of a horseshoe, was coapted to peroneal nerve distally, the other end was ligated As seen in the histomorphometric analysis, axons were observed to sprout towards both ends of the horseshoe-shaped nerve graft in a free fashion without being affected by the negative influences of the microenvironment.

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