scholarly journals Protective Effect of Nano-Vitamin C on Infertility due to Oxidative Stress Induced by Lead and Arsenic in Male Rats

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Mahdieh Raeeszadeh ◽  
Behzad Karimfar ◽  
Ali Akbar Amiri ◽  
Abolfazl Akbari
Keyword(s):  
Oxidative Stress ◽  
Vitamin C ◽  
Oxidative Damage ◽  
Protein Carbonyl ◽  
Sperm Parameters ◽  
Male Rats ◽  
Histological Evaluation ◽  
Hormonal Changes ◽  
Testosterone Levels ◽  
Carbonyl Protein

Occupational and environmental exposure to heavy metals such as arsenic (As) and lead (Pb) by inducing oxidative damage may impair male fertility. However, there is a new view that shows that the nano form of vitamins such as vitamin C, which have antioxidant activity, can be effective in improving this disorder. Therefore, this study aimed to evaluate the effect of NVC (NVC) on reproductive toxicity caused by the combination of Pb and As on testicular histology, sperm morphology, oxidative stress parameters, and hormonal changes in male rats. In this experimental study, forty-two male Wistar rats were randomly divided into six groups: control, NVC (200 mg/kg), As (50 ppm sodium arsenate), Pb (500 ppm Pb acetate), As + NVC, and Pb + NVC. FSH, LH, and testosterone levels were measured in serum. The activity of glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), carbonyl protein, malondialdehyde (MDA), and total antioxidant capacity (TAC) was measured in testis. Histological examination and sperm parameters were also evaluated. FSH, LH, and testosterone levels and sperm parameters significantly decreased, and levels of protein carbonyl, MDA, and DNA fragmentation increased in the As and Pb groups, while treatment with NVC could improve them. Histological evaluation and sperm parameters in As and Pb groups showed damage in the process of spermatogenesis and sperm parameters. The treatment with NVC could significantly improve these parameters. The activity of GPx, SOD, and CAT in testis decreased in As and Pb groups, while treatment with NVC could enhance them. It can be concluded that NVC by inhibiting oxidative damage and improving serum level of testosterone, LH, and FSH could overcome As- and Pb-induced reproductive dysfunction.

scholarly journals Potential Protective Effect of Vitamin C on Qunalphos-Induced Cardiac Toxicity: Histological and Tissue Biomarker Assay

Biomedicines ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 39
Author(s):  
Ayed A. Shati ◽  
Mohamed Samir A. Zaki ◽  
Youssef A. Alqahtani ◽  
Mohamed A. Haidara ◽  
Mubarak Al-Shraim ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin C ◽  
Protective Effect ◽  
Cardiac Tissue ◽  
Electron Microscopic Examination ◽  
Male Rats ◽  
Cardiac Damage ◽  
Electron Microscopic ◽  
Necrosis Factor Alpha ◽  
Vit C

Insecticides and toxicants abound in nature, posing a health risk to humans. Concurrent exposure to many environmental contaminants has been demonstrated to harm myocardial performance and reduce cardiac oxidative stress. The purpose of this research was to study the protective effect of vitamin C (Vit C) on quinalphos (QP)-induced cardiac tissue damage in rats. Eighteen albino male rats were randomly categorised into three groups (n = 6). Control, QP group: rats received distilled water. QP insecticide treatment: an oral administration of QP incorporated in drinking water. QP + Vit C group: rats received QP and Vit C. All the experiments were conducted for ten days. Decline of cardiac antioxidant biomarkers catalase (CAT) and reduced glutathione (GPx) along with increased proinflammatory markers tumour necrosis factor-alpha (TNF-α) and interleukin 6 (IL-6) indicated oxidative and inflammatory damage to the heart following administration of QP when compared to control rats. The light microscopic and ultrastructure appearance of QP-treated cardiomyocytes exhibited cardiac damage. Administration of Vit C showed decreased oxidative and inflammatory biomarkers, confirmed with histological and electron microscopic examination. In conclusion, Vit C protected the heart from QP-induced cardiac damage due to decreased inflammation and oxidative stress.

The effects of oral glutamine on cyclophosphamide-induced nephrotoxicity in rats

2010 ◽  
Vol 30 (7) ◽  
pp. 616-623 ◽  
Author(s):  
Premila Abraham ◽  
Bina Isaac
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Body Weight ◽  
Renal Damage ◽  
Neutrophil Infiltration ◽  
Protein Carbonyl ◽  
Male Rats ◽  
Glutathione Depletion ◽  
Myeloperoxidase Activity ◽  
Protein Carbonyl Content

Nephrotoxicity is one of the adverse side effects of cyclophosphamide (CP) chemotherapy. In a recent study, we have demonstrated that oxidative stress and glutathione depletion play important roles in CP-induced renal damage. The aim of the study was to verify whether glutamine, the precursor for glutathione synthesis, prevents CP-induced oxidative stress and renal damage using a rat model. Adult male rats were administered a single dose of 150 mg/ kg body weight of CP intraperitoneally. The glutamine-pretreated rats were administered 1 gm/kg body weight of glutamine orally 2 h before the administration of CP. Vehicle/glutaminetreated rats served as controls. All the rats were killed 16 h after the dose of CP/vehicle. The kidneys were removed and used for light microscopic and biochemical studies. The markers of oxidative stress including malondialdehyde content, protein carbonyl content, protein thiol, reduced glutathione and myeloperoxidase activity, a marker of neutrophil infiltration, were measured in kidney homogenates. CP treatment-induced damage to kidney involved the glomeruli and the tubules. Pretreatment with glutamine reduced CP-induced glutathione depletion and increased myeloperoxidase activity. However, it did not prevent CP-induced lipid peroxidation, protein carbonylation and renal damage. The results of the present study suggest that glutamine pretreatment does not prevent CP-induced lipid peroxidation and renal damage, although it prevents CP-induced glutathione depletion and neutrophil infiltration significantly. It is suggested that mechanisms other than oxidative stress may also be involved and/or oxidative stress may be consequence and not the cause of CP induced renal damage.

scholarly journals Toxic Effects of Trazodone on Male Reproductive System via Disrupting Hypothalamic-Pituitary-Testicular Axis and Inducing Testicular Oxidative Stress

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Sinem Ilgın ◽  
Gözde Aydoğan-Kılıç ◽  
Merve Baysal ◽  
Volkan Kılıç ◽  
Mina Ardıç ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Reproductive Toxicity ◽  
Sperm Concentration ◽  
Serum Testosterone ◽  
Toxic Effects ◽  
Male Rats ◽  
Hormonal Changes ◽  
Depression And Anxiety ◽  
Sperm Dna Damage

Depression and anxiety are recognized as public health problems. Epidemiological studies have shown that depression and anxiety often occur during reproductive ages between 20 and 60 years of age in males. Trazodone is one of the most frequently prescribed drugs in the treatment of depression and anxiety. Drugs used in repeated doses also play a role in the etiology of infertility. In our study, it was aimed to identify the possible toxic effects of trazodone on male rats and elucidate the underlying mechanisms. Vehicle or trazodone (5, 10, and 20 mg/kg/day) was administered to rats for 28 consecutive days (n=8 per group). At the end of that period, sperm concentration, motility, morphology, and DNA damage were determined and testicular morphology was assessed histopathologically in rats. Additionally, we investigated hormonal status by determining serum testosterone, FSH, and LH levels and oxidative stress by determining glutathione and malondialdehyde levels in testicular tissue to elucidate mechanisms of possible reproductive toxicity. According to our results, sperm concentration, sperm motility, and normal sperm morphology were decreased; sperm DNA damage was increased in trazodone-administered groups. Degenerative findings on the testicular structure were observed after trazodone administration in rats. Additionally, serum FSH, LH, and testosterone levels were elevated in the trazodone-administered groups. Increased MDA levels were the signs of enhanced oxidative stress after trazodone administration in testis tissues. Thus, we concluded that trazodone induced reproductive toxicity in male rats; this reproductive toxicity was accompanied by oxidative stress and hormonal changes, which are considered as important causes of reproductive disorders.

scholarly journals Effect of Running Exercise on Oxidative Stress Biomarkers: A Systematic Review

2021 ◽  
Vol 11 ◽  
Author(s):  
Anand Thirupathi ◽  
Ricardo A. Pinho ◽  
Ukadike C. Ugbolue ◽  
Yuhuan He ◽  
Yao Meng ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Oxidative Damage ◽  
Thiobarbituric Acid ◽  
Protein Carbonyl ◽  
Enzymatic Antioxidants ◽  
Oxidative Stress Biomarkers ◽  
Running Exercise ◽  
Exercise Induced ◽  
The Individual ◽  
Meta Analyses

Background: Exercise induced health benefits are limited by the overaccumulation of reactive oxygen species (ROS). ROS and further oxidative stress could potentially induce muscle damage which could result in poor exercise performance. However, predicting ROS induced oxidative stress in response to endurance training has several limitations in terms of selecting biomarkers that are used to measure oxidative stress.Objective: The purpose of this study was to systematically investigate the suitable biomarkers that predict oxidative stress status among runners.Methods: According to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement, a search for relevant articles was carried out on PubMed/Medline, ISI Web of Science, and Google Scholar using related search terms such as oxidative damage, ROS, exercise, physical training, running, marathon, and ultramarathon.Results: Outcomes included (1) running programs like a half-marathon, ultramarathon, and iron-man race, (2) measuring biochemical assessment of oxidative damage markers such as malondialdehyde (MDA), protein carbonyl (PC), total antioxidant capacity (TAC), thiobarbituric acid reactive substances (TBARS), 8-Oxo-2'-deoxyguanosine (8-OH-dG), 4-hydroxynonenal (HNE), and F1-isoprostones, and enzymatic and non-enzymatic antioxidants level.Conclusions: This study concluded that a running exercise does not elicit a response to specific biomarkers of oxidative stress, instead, oxidative damage markers of lipids, proteins, and various enzymatic and non-enzymatic antioxidants are expressed according to the training status of the individual.

scholarly journals In vivo evidences of Curcuma longa on oxidative stress in STZ-induced diabetes on sperm parameters in male wistar rats

2017 ◽  
Vol 6 (3) ◽  
pp. 1045-1051
Author(s):  
A.J. Olanrewaju ◽  
S.Y. Olatunji ◽  
J.O. Owolabi ◽  
A.T. Oluwatosin ◽  
W.C. Amaechi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Sperm Quality ◽  
Curcuma Longa ◽  
Diabetic Control ◽  
Positive Control ◽  
Sperm Parameters ◽  
Male Rats ◽  
Control Group ◽  
Alternative Source

Several conditions such as chemotherapy and toxins can interfere with spermatogenesis and reduce sperm quality and production. In the case where natural antioxidant response cannot manage oxidative stress and free radical overload, oxidative damage occurs and this begins the genesis of many diseases of which diabetes is one of them. This has awakened the interest of researchers to the use of an alternative source of medicine and herbal medicine. Medicinal use of Curcuma longa dates back to ancient China and India; its constituents are stated to have anti-hepatotoxic, anti-inflammatory, stimulant, and antioxidant and used since ancient time as medicinal and nutritive origins knowing to possess androgenic activities and have well effect in diseases treatment in more countries world-wide. As an antioxidant Curcuma longa possible has a useful effect on spermatogenesis and sperm parameters. Wistar male rats (n=24) were allocated into six groups, positive control (n=4), diabetic control (n=4) and experimental groups (n=20), that subdivided into groups of 4 that received treatment of Curcuma longa rhizome powder with or without STZ-induced diabetes in the dosages (25 and 100mg/kg/day) for 21 consecutive days. In twenty-second day, the testes were removed and semen was collected from epididymis and prepared for analysis. The percentage of sperm viability and motility in the treatment groups increased mildly with a significance of: (p<0.05) in comparison to control group and with the diabetic group being critically lower than those in control group. This suggested that Curcuma longa may be promising in enhancing sperm health parameters.Keywords: Turmeric, oxidative stress, semen, STZ-induced diabetes, antioxidant

Dietary vitamin C and E modulates oxidative stress induced-kidney and lens injury in diabetic aged male rats through modulating glucose homeostasis and antioxidant systems

2011 ◽  
Vol 29 (4) ◽  
pp. 287-293 ◽  
Author(s):  
Dilek Özkaya ◽  
Mustafa Nazıroğlu ◽  
Abdullah Armağan ◽  
Alpay Demirel ◽  
Banu Kale Köroglu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin C ◽  
Glucose Homeostasis ◽  
Male Rats ◽  
Dietary Vitamin ◽  
Antioxidant Systems

scholarly journals Acetaldehyde Content and Oxidative Stress in the Deleterious Effects of Alcohol Drinking on Rat Uterine Horn

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Lara Romina Buthet ◽  
María Eugenia Maciel ◽  
Leandro Néstor Quintans ◽  
Carmen Rodríguez de Castro ◽  
Martín Hernán Costantini ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Alcohol Exposure ◽  
Protein Carbonyl ◽  
Ethanol Exposure ◽  
Uterine Horn ◽  
Significant Finding ◽  
Hormonal Changes ◽  
Protein Carbonyl Content ◽  
Acetaldehyde Production ◽  
And Oxidative Stress

After alcohol exposure through a standard Lieber and De Carli diet for 28 days, a severe atrophy in the rat uteirne horn was observed, accompanied by significant alterations in its epithelial cells. Microsomal pathway of acetaldehyde production was slightly increased. Hydroxyl radicals were detected in the cytosolic fraction, and this was attributed to participation of xanthine oxidoreductase. They were also observed in the microsomal fraction in the presence of NADPH generating system. No generation of 1-hydroxyethyl was evidenced. Thet-butylhydroperoxide-induced chemiluminescence analysis of uterine horn homogenates revealed a significant increase in the chemiluminiscence emission due to ethanol exposure. In the animals repeatedly exposed to alcohol, sulfhydryl content from uterine horn proteins was decreased, but no significant changes were observed in the protein carbonyl content from the same samples. Minor but significant decreasing changes were observed in the GSH content accompanied by a tendency to decrease in the GSH/GSSG ratio. A highly significant finding was the diminished activity content of glutathione peroxidase. Results suggest that acetaldehyde accumulation plus the oxidative stress may play an additional effect to the alcohol-promoted hormonal changes in the uterus reported by others after chronic exposure to alcohol.

Estrogen administration, postexercise tissue oxidative stress and vitamin C status in male rats

1998 ◽  
Vol 76 (10-11) ◽  
pp. 952-960 ◽  
Author(s):  
Peter M Tiidus ◽  
Eric Bombardier ◽  
Nick Hidiroglou ◽  
Rene Madere
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Vitamin E ◽  
Vitamin C ◽  
Glutathione Peroxidase ◽  
Antioxidant Status ◽  
Male Rats ◽  
Glutathione Status ◽  
Estrogen Administration ◽  
Tissue Vitamin

Estrogen can putatively act as an antioxidant and protect tissues from exercise-induced oxidative stress. To test the in vivo efficacy of estrogen, the effects of 2 weeks of daily estrogen (40 µg·kg-1 body weight beta-estradiol 3-benzoate) injection on indices of immediate postexercise oxidative stress and antioxidant status were determined in adult male rats, with and without 8 weeks of prior dietary vitamin E deprivation. The treadmill running protocol (60 min at 21 m·min-1, 12% grade) induced significant oxidative stress as indicated by muscle glutathione status. Estrogen administration had little effect on postexercise tissue glutathione status, superoxide dismutase and glutathione peroxidase activity, and vitamin E levels. Estrogen administration induced significant reductions in muscle, liver, and heart vitamin C concentrations following exercise, as well as in unexercised male rats. Tissue vitamin C loss was not directly mediated through liver glycogen or glutathione status. Thus, estrogen administration generally did not appear to influence postexercise tissue indices of oxidative stress or antioxidant status and may have contributed to a decline in overall antioxidant protection by inducing losses in tissue vitamin C content.Key words: glutathione, vitamin E, muscle, superoxide dismutase, glutathione peroxidase.

scholarly journals Study the Effect of Vitamins (C and E) on Oxidative Stress and Antioxidants Changes Induced by VCM in Male Rats

2020 ◽  
Vol 11 (03) ◽  
pp. 430-434
Author(s):  
Shaymaa J. Shamran ◽  
Haider S. Jaffat
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Vitamin C ◽  
Male Rats ◽  
Control Group ◽  
Protective Effects ◽  
Vancomycin Group ◽  
Animal House ◽  
Antioxidant Effects ◽  
Oxidative Effect

The current study was designed to determine the antioxidant effects of vitamin C and vitamin E against oxidative stress induced by vancomycin in some antioxidants changes in the male rats. The study was conducted in the animal house of the Faculty of Science/University of Kufa for the period from April, 2018 to May, 2018 on 119 animals of male rats aged 2.5–3 months and the weight of 150-200 gm. Two experiments designed in this study addressed the first and two experiments to study the oxidative effect of vancomycin in addition to the protective effects of vitamin C and vitamin E to reduce these effects in the treatment of animals for one week and three weeks with vancomycin and vancomycin plus vitamins. The results indicated a significant increase (p less than 0.05) in the MDA, CAT, and significant decrease (p less than 0.05) in SOD, and GPX. In the animals treated with vancomycin 40,60 mg/kg only compared to the control group for the two periods of administration at the same time occur a significant decrease(p less than 0.05) in the MDA, CAT and a significant increase (p less than 0.05) in the SOD and GPX after treated animals with vancomycin 40,60 mg/kg with vitamin C and vitamin E for a period of one and three weeks compared with vancomycin group.

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