scholarly journals A Practical Method for Determination of Nine Nucleosides in Tricholoma matsutake by UPLC/MS and Quantitative Analysis of Multicomponents Using Single Marker Method

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Li Yong ◽  
An-Qin Leng ◽  
Zhi-Xiang Yang ◽  
Ying Xue
Keyword(s):  
Quantitative Analysis ◽  
Standard Method ◽  
Practical Method ◽  
Ultra Performance Liquid Chromatography ◽  
Assessment Method ◽  
Tricholoma Matsutake ◽  
Internal Reference ◽  
External Standard Method ◽  
External Standard ◽  
Single Marker

Nucleosides can be used as quality evaluation indicators of Tricholoma matsutake. In this work, a new ultra-performance liquid chromatography-tandem mass spectrometry (UPLC/MS) strategy for quantitative analysis of multiple components using a single marker (QAMS) was proposed to determine nine nucleosides (adenosine, cytidine, guanosine, inosine, uridine, 2′-deoxyadenosine, 2′-deoxycytidine, 2′-deoxyguanosine, and 2′-deoxyuridine) in T. matsutake. Guanosine was set as the internal reference substance, whose content in T. matsutake was determined using the conventional external standard method. Relative correction factors (RCFs) between guanosine and eight other nucleosides were measured. The concentrations of the eight components were calculated with the obtained RCFs by QAMS. An ultrasonic extraction method is used for sample preparation. This method was validated to be sensitive, precise, and accurate with the LODs of 0.31–1.9 ng, the overall intraday and interday variations less than 4.08%, and the overall recovery over 89.0%. The correlation coefficients (r2) of the calibration curves were higher than 0.9918. The values of vector angle analysis were above 0.99845, which indicates no significant differences between the conventional external standard method and the present QAMS method. As far as we know, this is also the first report of UPLC/MS analysis of nucleoside compounds by QAMS, providing an efficient and feasible quality assessment method for other natural products containing nucleosides.

scholarly journals Quality Evaluation of Polar and Active Components in Crude and ProcessedFructus Corniby Quantitative Analysis of Multicomponents with Single Marker

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Yuhong Jiang ◽  
Hui Chen ◽  
Liling Wang ◽  
Jing Zou ◽  
Xiao Zheng ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Active Components ◽  
Internal Reference ◽  
External Standard Method ◽  
Significant Difference ◽  
External Standard ◽  
Single Marker ◽  
Quantitative Results ◽  
Processed Products

Objective. To develop a quantitative analysis of multicomponents by single-marker (QAMS) method for the simultaneous determination of polar active components inFructus Corni.Methods. Loganin was selected as the internal reference, and the relative correction factors (RCFs) of gallic acid, 5-hydroxymethyl-2-furfural, morroniside, sweroside, cornin, 7α-O-methylmorroniside, 7β-O-methylmorroniside, 7α-O-ethylmorroniside, 7β-O-ethylmorroniside, and cornuside were established. The contents of multicomponents were then calculated based on their RCFs, respectively. Contents of the 11 components were also calculated by external standard method and compared with those of the QAMS method.Results.The contents of the 11 components in 21 crude and 10 processedFructus Corniproducts were measured. No significant difference was found in the quantitative results of the QAMS and external standard methods.Conclusion. QAMS could serve as an accurate and convenient method in determining the polar and active components inFructus Corniand its processed products.

scholarly journals Analysis of the HPLC Fingerprint and QAMS for Sanhuang Gypsum Soup

2018 ◽  
Vol 2018 ◽  
pp. 1-14 ◽  
Author(s):  
Yi Peng ◽  
Minghui Dong ◽  
Jing Zou ◽  
Zhihui Liu
Keyword(s):  
Quantitative Analysis ◽  
Standard Method ◽  
Evaluation Method ◽  
Similarity Analysis ◽  
Hplc Fingerprint ◽  
External Standard Method ◽  
Significant Difference ◽  
External Standard ◽  
Single Marker

A valid and encyclopaedic evaluation method for assessing the quality of Sanhuang Gypsum Soup (SGS) has been set up based on analysis of high-performance liquid chromatography (HPLC) fingerprint combined with the quantitative analysis of multicomponents by single marker (QAMS) method, hierarchical cluster analysis (HCA), and similarity analysis. 20 peaks of the common model were obtained and used for the similarity analysis and HCA analysis. Berberine was selected as an internal reference, and the relative correction factors of mangiferin, geniposide, liquiritin, epiberberine, coptisine, baicalin, palmatine, harpagosid, wogonoside, cinnamic acid, cinnamic aldehyde, baicalein, glycyrrhizic acid, and wogonin were established. The accuracy of quantitative analysis of multicomponents by the single-marker method was verified by comparing the contents of the fourteen components calculated by the external standard method with those of the quantitative analysis of multicomponents by the single-marker method. No significant difference was found in the quantitative results of the established quantitative analysis of multicomponents by a single-marker method and an external standard method. In summary, these methods were applied to evaluate the quality of SGS successfully. As a result, these evaluation methods have great potential to be widely used in the quality control of traditional Chinese medicines (TCM).

scholarly journals Simultaneous Determination of Six Active Components in Oviductus Ranae via Quantitative Analysis of Multicomponents by Single Marker

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Shihan Wang ◽  
Yang Xu ◽  
Yanwei Wang ◽  
Huailei Yang ◽  
Zuying Lv ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Standard Method ◽  
Internal Standard ◽  
Active Components ◽  
External Standard Method ◽  
Significant Difference ◽  
External Standard ◽  
Single Marker ◽  
Quantitative Results ◽  
Oviductus Ranae

A method, quantitative analysis of multicomponents by single marker (QAMS), was established in this article to investigate the quality control of a traditional Chinese medicine, Oviductus Ranae. 7-Hydroxycholesterol, 7-ketocholesterol, 4-cholesten-3-one, stigmasterol, 7-dehydrocholesterol, and cholesterol were selected as the indexes of quality evaluation of Oviductus Rana. The determination was achieved on an Agilent HC-C18 column (4.6 mm × 250 mm, 5 μm) using methanol with water (87 : 13 v/v) as mobile phase at the flow rate of 2.0 mL/min. Cholesterol was used as an internal standard to determine the relative correction factors between cholesterol and other steroidal constituents in Oviductus Ranae. The contents of those steroidal constituents were calculated at the same time. To evaluate the QAMS method, an external standard method was used to determine the contents of six steroidal constituents. No significant difference was observed when comparing the quantitative results of QAMS with the results of external standard method. The proposed QAMS method was proved to be accurate and feasible based on methodological experiments. QAMS provided a simple, efficient, economical, and accurate way to control the quality of Oviductus Ranae.

scholarly journals Quality Evaluation of Gastrodia Elata Tubers Based on HPLC Fingerprint Analyses and Quantitative Analysis of Multi-Components by Single Marker

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1521 ◽  
Author(s):  
Yehong Li ◽  
Yiming Zhang ◽  
Zejun Zhang ◽  
Yupiao Hu ◽  
Xiuming Cui ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Evaluation Method ◽  
Herbal Medicines ◽  
Assessment Method ◽  
Gastrodia Elata ◽  
Clinical Safety ◽  
Hplc Fingerprint ◽  
External Standard Method ◽  
Significant Difference ◽  
Single Marker

Gastrodia elata (G. elata) tuber is a valuable herbal medicine used to treat many diseases. The procedure of establishing a reasonable and feasible quality assessment method for G. elata tuber is important to ensure its clinical safety and efficacy. In this research, an effective and comprehensive evaluation method for assessing the quality of G. elata has been developed, based on the analysis of high performance liquid chromatography (HPLC) fingerprint, combined with the quantitative analysis of multi-components by single marker (QAMS) method. The contents of the seven components, including gastrodin, p-hydroxybenzyl alcohol, p-hydroxy benzaldehyde, parishin A, parishin B, parishin C, and parishin E were determined, simultaneously, using gastrodin as the reference standard. The results demonstrated that there was no significant difference between the QAMS method and the traditional external standard method (ESM) (p > 0.05, RSD < 4.79%), suggesting that QAMS was a reliable and convenient method for the content determination of multiple components, especially when there is a shortage of reference substances. In conclusion, this strategy could be beneficial for simplifying the processes in the quality control of G. elata tuber and giving references to promote the quality standards of herbal medicines.

scholarly journals Systematic Study on a Quantitative Analysis of Multicomponents by Single Marker (QAMS) Method for Simultaneous Determination of Eight Constituents in Pneumonia Mixture by UPLC-MS/MS

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Haibo Zhang ◽  
Weina Xie ◽  
Jiangyun Liu ◽  
Xiaoqiang Xiang ◽  
Shilei Zhang ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Quality Evaluation ◽  
Acute Bronchitis ◽  
Internal Reference ◽  
Relative Retention ◽  
External Standard Method ◽  
New Strategy ◽  
Single Marker ◽  
Good Repeatability ◽  
And Control

Pneumonia mixture was formulated and is available to treat children acute pneumonia and acute bronchitis in our hospital for nearly forty years, but there are few studies of its quality evaluation or control. In this paper, a new strategy for quality evaluation of pneumonia mixture was explored and verified through qualitative and quantitative analyses of multicomponents by single marker (QAMS) by UPLC-MS/MS. Baicalein was selected as an internal reference, and the relative correction factors (RCFs) and the relative retention time (RRT) of (R, S)-goitrin, amygdalin, chlorogenic acid, pseudoephedrine hydrochloride, ephedrine hydrochloride, ammonium glycyrrhizinate, and baicalin were established. The robustness and durability of the QAMS method were investigated. RCF values calculated by the average (AVG) method and linear regression (LRG) method had good repeatability and were acceptable for quantitative analysis, and the RTT combined with the exact masses of precursor and fragment ions and their abundance could be adopted for accurately positioning the chromatographic peak of the eight constituents. The consistency and feasibility of the QAMS method were verified by comparing the contents of the seven components calculated by a classic and validated external standard method (ESM) with those of the QAMS method, which reduces analytical cost and time of detection and avoids the problem of the diversity and large quantity of reference standards. The results demonstrated that the QAMS method developed in this paper could provide a new, alternative, and promising method to comprehensively and effectively determine multicomponents and control the quality of pneumonia mixture or even a group of similar medicines.

scholarly journals Simultaneous Determination of Four Ingredients in Plantago Depressa by Single Marker

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Yang Xu ◽  
Yuejie Wang ◽  
Shengnan Bi ◽  
Yanxue Jia ◽  
Huiwei Bao
Keyword(s):  
Simultaneous Determination ◽  
Standard Method ◽  
Protocatechuic Acid ◽  
Correction Factors ◽  
Active Components ◽  
Relative Correction ◽  
External Standard Method ◽  
External Standard ◽  
Single Marker

Objective. To establish a quantitative analysis of multicomponents by single marker (QAMS) method for the simultaneous determination of 4 active components such as protocatechuic acid, catechin, quercetin, and luteolin in Plantago depressa. Method. 4 active components in Plantago depressa were studied. Quercetin was used as an internal reference to establish the relative correction factors among protocatechuic acid, catechin, and luteolin and calculate the contents of each component; the results were compared with those measured by the external standard method. Results. 4 components showed a good linear relationship in their respective concentration ranges (r > 0.9995). The relative correction factors (fs/k) of protocatechuic acid, catechin, and luteolin were 1.1992, 0.8613, and 1.6069, respectively. The method had good durability. The contents of protocatechuic acid, catechin, and luteolin calculated by QAMS were not significantly different from those measured by the external standard method. Conclusion. QAMS can be used to determine the content of 4 components in Plantago depressa at the same time, and the method is simple, accurate, and can be used for quality control.

scholarly journals Simultaneous Determination of Six Active Components in Danggui Kushen Pills via Quantitative Analysis of Multicomponents by Single Marker

2019 ◽  
Vol 2019 ◽  
pp. 1-11
Author(s):  
Huiwei Bao ◽  
Jihong Chi ◽  
Huailei Yang ◽  
Fangxin Liu ◽  
Kuo Fang ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Gallic Acid ◽  
Quality Evaluation ◽  
Evaluation Method ◽  
Internal Standard ◽  
Active Components ◽  
External Standard Method ◽  
Significant Difference ◽  
External Standard ◽  
Single Marker

In this paper, a valid evaluation method for the quality control of Danggui Kushen pills (DKP) has been established based on quantitative analysis of multicomponents by single marker (QAMS). Gallic acid, matrine, oxymatrine, catechin, ferulic acid, and rutin were selected as the indexes for quality evaluation of DKP. The analysis was achieved on an Agilent ZORBAX SB-C18 column (250  mm × 4.6  mm, 5 μm) via gradient elution. Gallic acid was used as internal standard to determine the relative correction factors (RCF) between gallic acid and other five constituents in DKP. The contents of those components were calculated at the same time. The accuracy of QAMS method was verified by comparing the contents of six components calculated by external standard (ES) method with those of the QAMS method. It turned out that there was no significant difference between the quantitative results of QAMS method and external standard method. The proposed QAMS method was proved to be accurate and feasible according to methodological experiments, which provided an accurate, efficient, and economical approach for quality evaluation of DKP.

scholarly journals Application of fingerprint combined with quantitative analysis and multivariate chemometric methods in quality evaluation of dandelion ( Taraxacum mongolicum )

2021 ◽  
Vol 8 (10) ◽  
Author(s):  
Chunying Li ◽  
Yao Tian ◽  
Chunjian Zhao ◽  
Shen Li ◽  
Tingting Wang ◽  
...  
Keyword(s):  
Quality Control ◽  
Quantitative Analysis ◽  
Herbal Medicines ◽  
Hierarchical Cluster ◽  
Assessment Method ◽  
Chemometric Methods ◽  
External Standard Method ◽  
Significant Difference ◽  
Quality Assessment Method ◽  
Single Marker

A quality assessment method based on quantitative analysis of multi-components by single marker (QAMS) and fingerprint was constructed from 15 batches of dandelion ( Taraxacum mongolicum ), using multivariate chemometric methods (MCM). MCM were established by hierarchical cluster analysis (HCA) and factor analysis (FA). HCA was especially performed using the R language and SPSS 22.0 software. The relative correction factors of chlorogenic acid, caffeic acid, p-coumaric acid, luteolin and apigenin were calculated with cichoric acid as a reference, and their contents were determined. The differences between external standard method (ESM) and QAMS were compared. There was no significant difference ( t -test, p > 0.05) in quantitative determination, proving the consistency of the two methods (QAMS and ESM). Dandelion material from Yuncheng, Shandong was used as a reference chromatogram. The fingerprints in 15 batches of dandelion were established by HPLC analysis. The similarity of the fingerprints in different batches of dandelion material was greater than or equal to 0.82. A total of 10 common peaks were identified. This strategy is simple, rapid and efficient in multiple component detection of dandelion. It is beneficial in simplifying dandelion's quality control processes and providing references to enhance quality control for other herbal medicines.

scholarly journals Carotenoid Contents of Lycium barbarum: A Novel QAMS Analyses, Geographical Origins Discriminant Evaluation, and Storage Stability Assessment

Molecules ◽  
2021 ◽  
Vol 26 (17) ◽  
pp. 5374
Author(s):  
Ruru Ren ◽  
Yanting Li ◽  
Huan Chen ◽  
Yingli Wang ◽  
Lingling Yang ◽  
...  
Keyword(s):  
Standard Method ◽  
Geographical Location ◽  
Storage Condition ◽  
Lycium Barbarum ◽  
External Standard Method ◽  
External Standard ◽  
Single Marker ◽  
And Storage ◽  
Zeaxanthin Dipalmitate ◽  
Β Carotene

Given the standard substances of zeaxanthin and its homologues obtained from Lycium barbarum L. (LB) are extremely scarce and unstable, a novel quantitative analysis of carotenoids by single marker method, named QAMS, was established. Four carotenoids including lutein, zeaxanthin, β-carotene, and zeaxanthin dipalmitate were determined simultaneously by employing trans-β-apo-8′-carotenal, a carotenoid component which did not exist in LB, as standard reference. Meanwhile, β-carotene, another carotenoid constituent which existed in LB, was determined as contrast. The QAMS methods were fully verified and exhibited low standard method difference with the external standard method (ESM), evidenced by the contents of four carotenoids in 34 batches of LB samples determined using ESM and QAMS methods, respectively. HCA, PCA, and OPLS-DA analysis disclosed that LB samples could be clearly differentiated into two groups: one contained LB samples collected from Ningxia and Gansu; the other was from Qinghai, which was directly related to the different geographical location. Once exposed under high humidity (RH 75 ± 5%) at a high temperature (45 ± 5 °C) as compared with ambient temperature (25 ± 5 °C), from day 0 to day 28, zeaxanthin dipalmitate content was significantly decreased, and ultimately, all the decrease rates reached about 80%, regardless of the storage condition. Our results provide a good basis for improving the quality control of LB.

scholarly journals A Fast and Validated HPLC Method for the Simultaneous Analysis of Five 5-HT3 Receptor Antagonists via the Quantitative Analysis of Multicomponents by a Single Marker

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Fuchao Chen ◽  
Baoxia Fang ◽  
Peng Li ◽  
Sicen Wang
Keyword(s):  
Quantitative Analysis ◽  
High Performance ◽  
Dosage Form ◽  
Potassium Dihydrogen Phosphate ◽  
Dihydrogen Phosphate ◽  
Receptor Antagonists ◽  
Internal Reference ◽  
External Standard Method ◽  
Significant Difference ◽  
Single Marker

In this study, a new strategy for the simultaneous quantization of five serotonin 5-hydroxytryptamine receptor antagonists—ondansetron, azasetron, ramosetron, granisetron, and tropisetron—either in infusion samples or in injection dosage form was first established based on high-performance liquid chromatography combined with a quantitative analysis of multiple components by a single marker. The quantitative analysis of multicomponents by a single marker method was conducted with ondansetron as an internal reference substance and performed using relative retention time and ultraviolet spectral similarity as the double indicator. The quantitative analysis of the 5-HT3 receptor antagonists was calculated and investigated based on the relative correction factors. Chromatographic separation was achieved using a C18 column (150 mm × 4.6 mm, 5.0 μm), and the mobile phase was composed of acetonitrile-0.05 mol·L−1 potassium dihydrogen phosphate (pH 4.0) (25 : 75) at a flow rate of 1.0 mL·min−1 and detection wavelengths of 307 nm (ondansetron, azasetron, ramosetron), 302 nm (granisetron), and 285 nm (tropisetron). In addition, the accuracy of the quantitative analysis of multicomponents by a single marker method was compared with an external standard method, and no significant difference was observed between the two methods. The established method is rapid, is easy, and does not require many reference substances, and it can been successfully applied as part of the quality control of the five 5-HT3 receptor antagonists in their injection dosage form and infusion sample drugs in hospitals.

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