scholarly journals Simultaneous Determination of Six Active Components in Oviductus Ranae via Quantitative Analysis of Multicomponents by Single Marker

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Shihan Wang ◽  
Yang Xu ◽  
Yanwei Wang ◽  
Huailei Yang ◽  
Zuying Lv ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Standard Method ◽  
Internal Standard ◽  
Active Components ◽  
External Standard Method ◽  
Significant Difference ◽  
External Standard ◽  
Single Marker ◽  
Quantitative Results ◽  
Oviductus Ranae

A method, quantitative analysis of multicomponents by single marker (QAMS), was established in this article to investigate the quality control of a traditional Chinese medicine, Oviductus Ranae. 7-Hydroxycholesterol, 7-ketocholesterol, 4-cholesten-3-one, stigmasterol, 7-dehydrocholesterol, and cholesterol were selected as the indexes of quality evaluation of Oviductus Rana. The determination was achieved on an Agilent HC-C18 column (4.6 mm × 250 mm, 5 μm) using methanol with water (87 : 13 v/v) as mobile phase at the flow rate of 2.0 mL/min. Cholesterol was used as an internal standard to determine the relative correction factors between cholesterol and other steroidal constituents in Oviductus Ranae. The contents of those steroidal constituents were calculated at the same time. To evaluate the QAMS method, an external standard method was used to determine the contents of six steroidal constituents. No significant difference was observed when comparing the quantitative results of QAMS with the results of external standard method. The proposed QAMS method was proved to be accurate and feasible based on methodological experiments. QAMS provided a simple, efficient, economical, and accurate way to control the quality of Oviductus Ranae.

scholarly journals Quality Evaluation of Polar and Active Components in Crude and ProcessedFructus Corniby Quantitative Analysis of Multicomponents with Single Marker

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Yuhong Jiang ◽  
Hui Chen ◽  
Liling Wang ◽  
Jing Zou ◽  
Xiao Zheng ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Active Components ◽  
Internal Reference ◽  
External Standard Method ◽  
Significant Difference ◽  
External Standard ◽  
Single Marker ◽  
Quantitative Results ◽  
Processed Products

Objective. To develop a quantitative analysis of multicomponents by single-marker (QAMS) method for the simultaneous determination of polar active components inFructus Corni.Methods. Loganin was selected as the internal reference, and the relative correction factors (RCFs) of gallic acid, 5-hydroxymethyl-2-furfural, morroniside, sweroside, cornin, 7α-O-methylmorroniside, 7β-O-methylmorroniside, 7α-O-ethylmorroniside, 7β-O-ethylmorroniside, and cornuside were established. The contents of multicomponents were then calculated based on their RCFs, respectively. Contents of the 11 components were also calculated by external standard method and compared with those of the QAMS method.Results.The contents of the 11 components in 21 crude and 10 processedFructus Corniproducts were measured. No significant difference was found in the quantitative results of the QAMS and external standard methods.Conclusion. QAMS could serve as an accurate and convenient method in determining the polar and active components inFructus Corniand its processed products.

scholarly journals Simultaneous Determination of Six Active Components in Danggui Kushen Pills via Quantitative Analysis of Multicomponents by Single Marker

2019 ◽  
Vol 2019 ◽  
pp. 1-11
Author(s):  
Huiwei Bao ◽  
Jihong Chi ◽  
Huailei Yang ◽  
Fangxin Liu ◽  
Kuo Fang ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Gallic Acid ◽  
Quality Evaluation ◽  
Evaluation Method ◽  
Internal Standard ◽  
Active Components ◽  
External Standard Method ◽  
Significant Difference ◽  
External Standard ◽  
Single Marker

In this paper, a valid evaluation method for the quality control of Danggui Kushen pills (DKP) has been established based on quantitative analysis of multicomponents by single marker (QAMS). Gallic acid, matrine, oxymatrine, catechin, ferulic acid, and rutin were selected as the indexes for quality evaluation of DKP. The analysis was achieved on an Agilent ZORBAX SB-C18 column (250  mm × 4.6  mm, 5 μm) via gradient elution. Gallic acid was used as internal standard to determine the relative correction factors (RCF) between gallic acid and other five constituents in DKP. The contents of those components were calculated at the same time. The accuracy of QAMS method was verified by comparing the contents of six components calculated by external standard (ES) method with those of the QAMS method. It turned out that there was no significant difference between the quantitative results of QAMS method and external standard method. The proposed QAMS method was proved to be accurate and feasible according to methodological experiments, which provided an accurate, efficient, and economical approach for quality evaluation of DKP.

scholarly journals Exploitation of HPLC Analytical Method for Simultaneous Determination of Six Principal Unsaturated Fatty Acids in Oviductus Ranae Based on Quantitative Analysis of Multi-Components by Single-Marker (QAMS)

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 479
Author(s):  
Shihan Wang ◽  
Yuanshuai Gan ◽  
Hong Kan ◽  
Xinxin Mao ◽  
Yongsheng Wang
Keyword(s):  
Fatty Acids ◽  
Quantitative Analysis ◽  
Unsaturated Fatty Acids ◽  
Internal Standard ◽  
Active Components ◽  
Reliable Determination ◽  
External Standard Method ◽  
Single Marker ◽  
Oviductus Ranae

As one of the featured products in northeast China, Oviductus Ranae has been widely used as a nutritious food, which contains a variety of bioactive unsaturated fatty acids (UFAs). It is necessary to establish a scientific and reliable determination method of UFA contents in Oviductus Ranae. In this work, six principal UFAs in Oviductus Ranae, namely eicosapentaenoic acid (EPA), linolenic acid (ALA), docosahexaenoic acid (DHA), arachidonic acid (ARA), linoleic acid (LA) and oleic acid (OA), were identified using UPLC-MS/MS. The UFAs identified in Oviductus Ranae were further separated based on the optimized RP-HPLC conditions. Quantitative analysis of multi-components by single-marker (QAMS) method was implemented in content determination of EPA, ALA, DHA, ARA and OA, where LA was used as the internal standard. The experiments based on Taguchi design verified the robustness of the QAMS method on different HPLC instruments and chromatographic columns. The QAMS and external standard method (ESM) were used to calculate the UFA content of 15 batches of Oviductus Ranae samples from different regions. The relative error (r < 0.73%) and cosine coefficient showed that the two methods obtained similar contents, and the method validations met the requirements. The results showed that QAMS can comprehensively and effectively control the quality of UFAs in Oviductus Ranae which provides new ideas and solutions for studying the active components in Oviductus Ranae.

scholarly journals Analysis of the HPLC Fingerprint and QAMS for Sanhuang Gypsum Soup

2018 ◽  
Vol 2018 ◽  
pp. 1-14 ◽  
Author(s):  
Yi Peng ◽  
Minghui Dong ◽  
Jing Zou ◽  
Zhihui Liu
Keyword(s):  
Quantitative Analysis ◽  
Standard Method ◽  
Evaluation Method ◽  
Similarity Analysis ◽  
Hplc Fingerprint ◽  
External Standard Method ◽  
Significant Difference ◽  
External Standard ◽  
Single Marker

A valid and encyclopaedic evaluation method for assessing the quality of Sanhuang Gypsum Soup (SGS) has been set up based on analysis of high-performance liquid chromatography (HPLC) fingerprint combined with the quantitative analysis of multicomponents by single marker (QAMS) method, hierarchical cluster analysis (HCA), and similarity analysis. 20 peaks of the common model were obtained and used for the similarity analysis and HCA analysis. Berberine was selected as an internal reference, and the relative correction factors of mangiferin, geniposide, liquiritin, epiberberine, coptisine, baicalin, palmatine, harpagosid, wogonoside, cinnamic acid, cinnamic aldehyde, baicalein, glycyrrhizic acid, and wogonin were established. The accuracy of quantitative analysis of multicomponents by the single-marker method was verified by comparing the contents of the fourteen components calculated by the external standard method with those of the quantitative analysis of multicomponents by the single-marker method. No significant difference was found in the quantitative results of the established quantitative analysis of multicomponents by a single-marker method and an external standard method. In summary, these methods were applied to evaluate the quality of SGS successfully. As a result, these evaluation methods have great potential to be widely used in the quality control of traditional Chinese medicines (TCM).

scholarly journals Simultaneous Determination of Four Ingredients in Plantago Depressa by Single Marker

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Yang Xu ◽  
Yuejie Wang ◽  
Shengnan Bi ◽  
Yanxue Jia ◽  
Huiwei Bao
Keyword(s):  
Simultaneous Determination ◽  
Standard Method ◽  
Protocatechuic Acid ◽  
Correction Factors ◽  
Active Components ◽  
Relative Correction ◽  
External Standard Method ◽  
External Standard ◽  
Single Marker

Objective. To establish a quantitative analysis of multicomponents by single marker (QAMS) method for the simultaneous determination of 4 active components such as protocatechuic acid, catechin, quercetin, and luteolin in Plantago depressa. Method. 4 active components in Plantago depressa were studied. Quercetin was used as an internal reference to establish the relative correction factors among protocatechuic acid, catechin, and luteolin and calculate the contents of each component; the results were compared with those measured by the external standard method. Results. 4 components showed a good linear relationship in their respective concentration ranges (r > 0.9995). The relative correction factors (fs/k) of protocatechuic acid, catechin, and luteolin were 1.1992, 0.8613, and 1.6069, respectively. The method had good durability. The contents of protocatechuic acid, catechin, and luteolin calculated by QAMS were not significantly different from those measured by the external standard method. Conclusion. QAMS can be used to determine the content of 4 components in Plantago depressa at the same time, and the method is simple, accurate, and can be used for quality control.

scholarly journals Simultaneous Determination of Eight Chemical Components in Angelicae Sinensis Radix and Its Herbal Products by QAMS

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Yu Zhang ◽  
Qian Li ◽  
Yanmei Feng ◽  
Lan Yang ◽  
Qi Wang ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Ferulic Acid ◽  
Internal Standard ◽  
Chemical Components ◽  
Herbal Products ◽  
Standard Substance ◽  
External Standard Method ◽  
Significant Difference ◽  
Single Marker ◽  
Angelicae Sinensis

A HPLC method has been developed for simultaneously detecting chlorogenic acid, ferulic acid, senkyunolide I, senkyunolide H, coniferyl ferulate, senkyunolide A, ligustilide, and levistolide A in Angelicae Sinensis Radix through quantitative analysis of multicomponents by single-marker (QAMS) method with ferulic acid as internal standard substance. The relative analysis correction factors of each component in Angelicae Sinensis Radix have good reproducibility under different chromatography conditions. In addition, no significant difference of results was found between quantitative analysis of multicomponents by single-marker (QAMS) method and external standard method in determining content of these components of different Angelicae Sinensis Radix and its 12 kinds of preparations. As a result, the established QAMS method for Angelicae Sinensis Radix analysis with ferulic acid as internal standard substance is accurate and feasible, which could be used as an effective and economical method to control quality of Angelicae Sinensis Radix and its herbal products.

scholarly journals A Practical Method for Determination of Nine Nucleosides in Tricholoma matsutake by UPLC/MS and Quantitative Analysis of Multicomponents Using Single Marker Method

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Li Yong ◽  
An-Qin Leng ◽  
Zhi-Xiang Yang ◽  
Ying Xue
Keyword(s):  
Quantitative Analysis ◽  
Standard Method ◽  
Practical Method ◽  
Ultra Performance Liquid Chromatography ◽  
Assessment Method ◽  
Tricholoma Matsutake ◽  
Internal Reference ◽  
External Standard Method ◽  
External Standard ◽  
Single Marker

Nucleosides can be used as quality evaluation indicators of Tricholoma matsutake. In this work, a new ultra-performance liquid chromatography-tandem mass spectrometry (UPLC/MS) strategy for quantitative analysis of multiple components using a single marker (QAMS) was proposed to determine nine nucleosides (adenosine, cytidine, guanosine, inosine, uridine, 2′-deoxyadenosine, 2′-deoxycytidine, 2′-deoxyguanosine, and 2′-deoxyuridine) in T. matsutake. Guanosine was set as the internal reference substance, whose content in T. matsutake was determined using the conventional external standard method. Relative correction factors (RCFs) between guanosine and eight other nucleosides were measured. The concentrations of the eight components were calculated with the obtained RCFs by QAMS. An ultrasonic extraction method is used for sample preparation. This method was validated to be sensitive, precise, and accurate with the LODs of 0.31–1.9 ng, the overall intraday and interday variations less than 4.08%, and the overall recovery over 89.0%. The correlation coefficients (r2) of the calibration curves were higher than 0.9918. The values of vector angle analysis were above 0.99845, which indicates no significant differences between the conventional external standard method and the present QAMS method. As far as we know, this is also the first report of UPLC/MS analysis of nucleoside compounds by QAMS, providing an efficient and feasible quality assessment method for other natural products containing nucleosides.

scholarly journals Quality Evaluation of Gastrodia Elata Tubers Based on HPLC Fingerprint Analyses and Quantitative Analysis of Multi-Components by Single Marker

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1521 ◽  
Author(s):  
Yehong Li ◽  
Yiming Zhang ◽  
Zejun Zhang ◽  
Yupiao Hu ◽  
Xiuming Cui ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Evaluation Method ◽  
Herbal Medicines ◽  
Assessment Method ◽  
Gastrodia Elata ◽  
Clinical Safety ◽  
Hplc Fingerprint ◽  
External Standard Method ◽  
Significant Difference ◽  
Single Marker

Gastrodia elata (G. elata) tuber is a valuable herbal medicine used to treat many diseases. The procedure of establishing a reasonable and feasible quality assessment method for G. elata tuber is important to ensure its clinical safety and efficacy. In this research, an effective and comprehensive evaluation method for assessing the quality of G. elata has been developed, based on the analysis of high performance liquid chromatography (HPLC) fingerprint, combined with the quantitative analysis of multi-components by single marker (QAMS) method. The contents of the seven components, including gastrodin, p-hydroxybenzyl alcohol, p-hydroxy benzaldehyde, parishin A, parishin B, parishin C, and parishin E were determined, simultaneously, using gastrodin as the reference standard. The results demonstrated that there was no significant difference between the QAMS method and the traditional external standard method (ESM) (p > 0.05, RSD < 4.79%), suggesting that QAMS was a reliable and convenient method for the content determination of multiple components, especially when there is a shortage of reference substances. In conclusion, this strategy could be beneficial for simplifying the processes in the quality control of G. elata tuber and giving references to promote the quality standards of herbal medicines.

scholarly journals Analysis of the High-Performance Liquid Chromatography Fingerprints and Quantitative Analysis of Multicomponents by Single Marker of Products of Fermented Cordyceps sinensis

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Li-hua Chen ◽  
Yao Wu ◽  
Yong-mei Guan ◽  
Chen Jin ◽  
Wei-feng Zhu ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
High Performance ◽  
Cordyceps Sinensis ◽  
Array Detector ◽  
Hplc Fingerprint ◽  
External Standard Method ◽  
Significant Difference ◽  
Single Marker

Fermented Cordyceps sinensis, the succedaneum of Cordyceps sinensis which is extracted and separated from Cordyceps sinensis by artificial fermentation, is commonly used in eastern Asia in clinical treatments due to its health benefit. In this paper, a new strategy for differentiating and comprehensively evaluating the quality of products of fermented Cordyceps sinensis has been established, based on high-performance liquid chromatography (HPLC) fingerprint analysis combined with similar analysis (SA), hierarchical cluster analysis (HCA), and the quantitative analysis of multicomponents by single marker (QAMS). Ten common peaks were collected and analysed using SA, HCA, and QAMS. These methods indicated that 30 fermented Cordyceps sinensis samples could be categorized into two groups by HCA. Five peaks were identified as uracil, uridine, adenine, guanosine, and adenosine, and according to the results from the diode array detector, which can be used to confirm peak purity, the purities of these compounds were greater than 990. Adenosine was chosen as the internal reference substance. The relative correction factors (RCF) between adenosine and the other four nucleosides were calculated and investigated using the QAMS method. Meanwhile, the accuracy of the QAMS method was confirmed by comparing the results of that method with those of an external standard method with cosines of the angles between the groups. No significant difference between the two methods was observed. In conclusion, the method established herein was efficient, successful in identifying the products of fermented Cordyceps sinensis, and scientifically valid to be applicable in the systematic quality control of fermented Cordyceps sinensis products.

scholarly journals Preliminary Studies on Liquiritin, Deoxyschizandrin, and Tanshinone II A as Potential Anti-Neurodegenerative Disease Agent: Determination by Reverse-Phase Liquid Chromatography in Tianwang Buxin Pills

2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
Dan Xu ◽  
Sicen Wang ◽  
Xiaofang Hou ◽  
Changshan Sun
Keyword(s):  
Phosphoric Acid ◽  
Neurodegenerative Disease ◽  
Standard Method ◽  
Internal Standard ◽  
Acetonitrile Solution ◽  
Array Detector ◽  
Internal Standard Method ◽  
Tanshinone Ii A ◽  
External Standard Method ◽  
External Standard

Tianwang Buxin pill (TWBXP) is an ancient Chinese classic prescription. Liquiritin, deoxyschizandrin, and tanshinone II A are three bioactive components in TWBXP, which have been proven to be closely related to the therapy effect of neurodegenerative disease. Their contents are very low in TWBXP. In this study, we used a diode array detector (DAD) to perform a full wavelength scanning in order to choose a most suitable detection wavelength to establish an HPLC method for the simultaneous determination of these three components in TWBXP. Various chromatographic conditions were investigated to verify its applicability. Finally, a Kromasil C18 column (250 × 4.6 mm, 5 μm) thermostated at 30°C, mobile phase as 0.2% phosphoric acid solution (eluent A), and 0.1% phosphoric acid-acetonitrile solution (eluent B) were used. Both external standard method and internal standard method were used for quantification. The results showed that both methods were simple and convenient in operation without special pretreatment and exhibits excellent precision, repeatability (RSD < 3.0%), good linearity (R2 > 0.9990), and good recoveries (recovery value between 95% and 105%). Because of the low contents in samples, the internal standard method provided a better accurate result than the external standard method. The stability results showed the sample became stable within 24 hours at room temperature. The method provides a convenient and effective way for the quality control of TWBXP, and it can help the research about AD in the future.

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