scholarly journals Stimulation of Codonopsis pilosula Polysaccharide on Bifidobacterium of Human Gut Bacteria In Vitro

2021 ◽  
Vol 2021 ◽  
pp. 1-4
Author(s):  
Jiankuan Li ◽  
Lina Dong ◽  
Yue Liu ◽  
Jianping Gao
Keyword(s):  
16S Rrna ◽  
Human Health ◽  
Gut Bacteria ◽  
16S Rrna Sequencing ◽  
Human Gut ◽  
In Vitro Methods ◽  
Codonopsis Pilosula ◽  
Rrna Sequencing ◽  
Stimulation Of

Objective. To evaluate the prebiotic effects of Codonopsis pilosula polysaccharide (CPP) on human gut bacteria in vitro. Methods. Codonopsis Radix was extracted with water at 100°C, and the extract was precipitated by 80% ethanol to obtain CPP. Human fresh fecal samples were collected from three healthy adults and used to ferment CPP. The fermented samples were collected to be analyzed by 16S rRNA sequencing. Results. The results showed that CPP exhibited significantly the stimulation on the growth of genus Bifidobacterium of human gut bacteria (Padj < 0.05). Although CPP also exhibited regulative trends on the genera including Acidaminococcus, Bilophila, Dorea, and Eggerthella, no significant differences were observed (Padj > 0.05), which was likely associated with the limited samples (n = 3). Conclusion. CPP has the potential to stimulate the growth of Bifidobacterium of the human gut bacteria and to be benefit to human health.

scholarly journals LogMPIE, pan-India profiling of the human gut microbiome using 16S rRNA sequencing

2018 ◽  
Vol 5 (1) ◽  
Author(s):  
Ashok Kumar Dubey ◽  
Niyati Uppadhyaya ◽  
Pravin Nilawe ◽  
Neeraj Chauhan ◽  
Santosh Kumar ◽  
...  
Keyword(s):  
16S Rrna ◽  
Gut Microbiome ◽  
16S Rrna Sequencing ◽  
Human Gut ◽  
Human Gut Microbiome ◽  
Rrna Sequencing

scholarly journals The Pervasive Effects of an Antibiotic on the Human Gut Microbiota, as Revealed by Deep 16S rRNA Sequencing

PLoS Biology ◽  
2008 ◽  
Vol 6 (11) ◽  
pp. e280 ◽  
Author(s):  
Les Dethlefsen ◽  
Sue Huse ◽  
Mitchell L Sogin ◽  
David A Relman
Keyword(s):  
16S Rrna ◽  
Gut Microbiota ◽  
16S Rrna Sequencing ◽  
Human Gut ◽  
Human Gut Microbiota ◽  
Rrna Sequencing

scholarly journals Effect of Oregano Oil and Cobalt Lactate on Sheep In Vitro Digestibility, Fermentation Characteristics and Rumen Microbial Community

Animals ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 118
Author(s):  
Zhengwen Wang ◽  
Xiongxiong Li ◽  
Lingyun Zhang ◽  
Jianping Wu ◽  
Shengguo Zhao ◽  
...  
Keyword(s):  
16S Rrna ◽  
Relative Abundance ◽  
Rumen Fermentation ◽  
Gas Production ◽  
16S Rrna Sequencing ◽  
Molar Ratio ◽  
Fiber Digestibility ◽  
Rrna Sequencing ◽  
Fermentation Parameters

The objective of this experiment was to evaluate the effect of different EOC (0.1425% cobalt lactate + 1.13% oregano essential oil + 98.7275% carrier) levels on in vitro rumen fermentation and microbial changes. Six EOC levels (treatments: 0 mg·L−1, CON; 50 mg·L−1, EOC1; 100 mg·L−1, EOC2; 400 mg·L−1, EOC3; 800 mg·L−1, EOC4 and 1500 mg·L−1, EOC5) were selected to be used to in vitro incubation. The in vitro dry matter digestibility (IVDMD), in vitro neutral detergent fiber digestibility (IVNDFD), in vitro acid detergent fiber digestibility (IVNDFD), pH, ammonia-nitrogen (NH3-N) concentration, total volatile fatty acid (TVFA) concentration and microbial protein (MCP) concentration were measured after 48 h incubation, after which the groups with significant nutrient digestibility and fermentation parameters were subjected to 16S rRNA sequencing. The results showed that the total gas production (GP) of the EOC5 group was higher than that of the other groups after 12 h of in vitro incubation. TVFA, NH3-N and MCP concentrations were also shown to be higher in group EOC5 than those in other groups (p < 0.05), while NH3-N and MCP concentrations in the EOC2 group were lower than those in other groups significantly (p < 0.05). The molar ratio of acetic acid decreased while the molar ratio of propionic acid increased after the addition of EOC. 16S rRNA sequencing revealed that the rumen microbiota was altered in response to adding EOC, especially for the EOC5 treatment, with firmicutes shown to be the most abundant (43.1%). The relative abundance of Rikenellaceae_RC9_gut_group was significantly lower, while the relative abundance of uncultured_bacterium_f_Muribaculaceae and Succiniclasticum was significantly higher in the EOC5 group than those in other groups (p < 0.05). Comprehensive analysis showed that EOC (1500 mg·L−1) could significantly increase gas production, alter sheep rumen fermentation parameters and microbiota composition.

scholarly journals Echinacea purpurea Extract Affects the Immune System, Global Metabolome, and Gut Microbiome in Wistar Rats

2017 ◽  
Vol 9 (4) ◽  
pp. 1
Author(s):  
Chongchong Wang ◽  
Yuanlong Hou ◽  
Yingfang Lv ◽  
Shaoqiu Chen ◽  
Xueying Zhou ◽  
...  
Keyword(s):  
16S Rrna ◽  
Echinacea Purpurea ◽  
16S Rrna Sequencing ◽  
Young Rats ◽  
Treatment And Prevention ◽  
Rrna Sequencing ◽  
Immunologic Factors ◽  
The Status ◽  
Echinacea Purpurea Extract

Echinacea purpurea extract, a traditional herbal food additive with dual-purpose of medicine and edible material, has been widely used for the treatment and prevention of various infectious diseases, especially for children, old aged and immunocompromised patients. Although there were numerous reports suggested E. purpurea possessed immunostimulatory and antibacterial effects in vitro, the mechanisms underlying remained to be elucidated. This study employed immunologic factors analysis, GC-TOFMS based metabolomics and 16S-rRNA-sequencing microbiome profiling technologies to explore the effects of E. purpurea on young rats, a physiological insufficient immunity status, by compared with pidotimod treatment on young rats and adult animals. E. purpurea treatment significantly increased IL-2, decreased IL-6 and affect immunoglobulins in the spleen of young rats, indicating its promotion of cellular immunity. Both the immunologic factors and the global metabolome of E. purpurea treated young rats were close to the status of mature individuals. Results of 16S-rRNA-sequencing of ileum content together with co-metabolism metabolites demonstrated that E. purpurea changed gut microbiota structure characteristically as a reducing Firmicutes phylum, especially Lactobacillus, and a rising Actinobacteria phylum including Bifidobacterium. The results were concluded that E. purpurea could potentially promote the maturation of immune and metabolism of immature rats, and also affect gut flora structure.

Haemophilus parainfluenzaeInfective Endocarditis Diagnosed by Direct 16S rRNA Sequencing of Vegetation

2012 ◽  
Vol 2 (2) ◽  
pp. 111
Author(s):  
Sung-Hee Oh ◽  
Min-Chul Cho ◽  
Jae-Wook Kim ◽  
Dongheui An ◽  
Mun-Hui Jeong ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Sequencing ◽  
Rrna Sequencing

Full-length versus short amplicon 16S rRNA sequencing: benefits, drawbacks and risks

2020 ◽  
Author(s):  
Isabel Abellan-Schneyder ◽  
Andrea Janina Bayer ◽  
Sandra Reitmeier ◽  
Klaus Neuhaus
Keyword(s):  
16S Rrna ◽  
Full Length ◽  
16S Rrna Sequencing ◽  
Rrna Sequencing

Full-length versus short amplicon 16S rRNA sequencing: benefits, drawbacks and risks

2020 ◽  
Author(s):  
Andrea Janina Bayer ◽  
Sandra Reitmeier ◽  
Klaus Neuhaus ◽  
Isabel Abellan-Schneyder
Keyword(s):  
16S Rrna ◽  
Full Length ◽  
16S Rrna Sequencing ◽  
Rrna Sequencing
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