scholarly journals lncRNA FGD5-AS1 and miR-130a Can Be Used for Prognosis Analysis of Patients with Chronic Periodontitis

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Miao Yu ◽  
Chunyuan Chi
Keyword(s):  
Risk Factors ◽  
Chronic Periodontitis ◽  
Prognostic Value ◽  
Periodontal Pocket ◽  
Prognostic Indicators ◽  
Control Group ◽  
Expression Levels ◽  
Patient Prognosis ◽  
One Year ◽  
Prognostic Risk Factors

Background. lncRNA and microRNA affect the occurrence and development of many diseases, so they are expected to become diagnostic or predictive indicators. But the relationship between lncRNA FGD5-AS1 and miR-130a and the prognosis of chronic periodontitis is still unclear. The purpose of this study is to explore the prognostic value of the two in chronic periodontitis. Objective. This study set out to investigate the prognostic value of lncRNA FGD5-AS1 and miR-130a in chronic periodontitis. Methods. Eighty-seven patients with chronic periodontitis who visited our hospital from March 2016 to August 2017 were collected as an observation group (OG), and 72 subjects with periodontal health who underwent physical examination at the same time were collected as a control group (CG). The FGD5-AS1 and miR-130a expression levels of subjects in the two groups were compared, and prognosis of 87 patients who were reviewed one year later was counted. The expression levels of patients with different prognoses were compared when they were admitted to our hospital. We drew the ROC curve and explored the prognostic value of FGD5-AS1 and miR-130a. The risk factors for adverse prognosis were analyzed through logistic regression. Results. FGD5-AS1 was lowly expressed in patients, while miR-130a was highly expressed. FGD5-AS1 and miR-130a had certain diagnostic and predictive value in chronic periodontitis and patient prognosis. The higher the periodontal pocket, the higher the attachment loss. Lower FGD5-AS1 and higher miR-130a levels were independent prognostic risk factors. Conclusion. lncRNA FGD5-AS1 is lowly expressed in patients with chronic periodontitis, while miR-130a is highly expressed. Both of them have certain diagnostic and prognostic value in chronic periodontitis and may be potential diagnostic and prognostic indicators.

scholarly journals Analysis on the Expression and Prognostic Value of LncRNA FAF in Patients with Coronary Heart Disease

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Hai Xu ◽  
Xiwen Zhang ◽  
Kun Yu ◽  
Gang Zhang ◽  
Yafei Shi ◽  
...  
Keyword(s):  
Risk Factors ◽  
Coronary Heart Disease ◽  
Heart Disease ◽  
Poor Prognosis ◽  
Prognostic Value ◽  
High Sensitivity ◽  
Control Group ◽  
Coronary Lesion ◽  
Tnf Α ◽  
Factor Α

Objective. To investigate the expression and prognostic value of LncRNA FAF in patients with coronary heart disease. Patients and Methods. 97 patients with coronary heart disease who came to our hospital were selected as the research group (RG), and 97 healthy people who came to our hospital for physical examination during the same period were selected as the control group (CG). The serum LncRNA FAF, plasma homocysteine (HCY), lipoprotein A (Lp-a), serum tumor necrosis factor α (TNF-α), and high-sensitivity C-reactive protein (hsCRP) in the two groups of patients were detected, and their correlations were analyzed. Then, the predictive value and risk factors of FAF for poor prognosis of patients with coronary heart disease were analyzed. Results. The expression of LncRNA FAF in the serum of patients in the RG was significantly lower than that in the CG, and the expressions of HCY, Lp-a, TNF-α, and hsCRP were significantly higher than those in the CG (p <0.05). The AUC of FAF in the diagnosis of coronary heart disease was more than 0.9. FAF was negatively correlated with the coronary lesion vessels, HCY, Lp-a, TNF-α, and hsCRP expressions in patients with coronary heart disease ( p < 0.05 ). The ROC of FAF for predicting poor prognosis in patients with coronary heart disease was greater than 0.9. Low expression of FAF; high expressions of HCY, Lp-a, and hsCRP; and increase of coronary lesion vessels were independent risk factors for poor prognosis in patients with coronary heart disease. Conclusions. LncRNA FAF was lowly expressed in the serum of patients with coronary heart disease, and it was of high value in the diagnosis and prediction of poor prognosis of coronary heart disease. It was also an independent risk factor for poor prognosis of patients with coronary heart disease and may be a potential target for diagnosis and treatment of coronary heart disease.

scholarly journals Effect of Non-Surgical Periodontal Therapy on Superoxide Dismutase Levels in Gingival Tissues of Chronic Periodontitis Patients: A Clinical and Spectophotometric Analysis

2013 ◽  
Vol 34 (5) ◽  
pp. 305-311 ◽  
Author(s):  
Laxmi Sukhtankar ◽  
Anita Kulloli ◽  
Rahul Kathariya ◽  
Sharad Shetty
Keyword(s):  
Superoxide Dismutase ◽  
Chronic Periodontitis ◽  
Inflammatory Cells ◽  
Periodontal Therapy ◽  
Periodontal Pocket ◽  
Gingival Tissue ◽  
Control Group ◽  
Clinical Parameters ◽  
Sod Activity ◽  
Tissue Samples

BACKGROUND: Superoxide dismutase (SOD), an antioxidant acting against superoxide (oxygen radical, O2.-), it is released in inflammatory pathways and causes connective tissue breakdown. Increased SOD activity in inflamed gingiva may indicate increased O2.-radical generation by neutrophils and other inflammatory cells at the diseased site. The aim of the study was to evaluate the effects of non-surgical periodontal therapy (NSPT) on SOD levels in gingival tissues of chronic periodontitis patients.METHODS: Forty subjects: 20 periodontally healthy (Control) and 20 chronic periodontitis (Test); age range 24–55 years were recruited. Gingival tissue samples were collected by excising the inner lining of the periodontal pocket at baseline (prior to non-surgical periodontal therapy) and 2 months post therapy. In controls, tissue samples were obtained immediately after tooth extraction scheduled for orthodontic reasons. Clinical parameters included probing depth, clinical attachment level, gingival index, bleeding index, plaque index. SOD activities were assessed spectrophotometrically at baseline and 2 months post NSPT, results were analysed statistically.RESULTS: At baseline, patients with chronic periodontitis had higher mean SOD activity (2.73 ± 1.36) than the control subjects (1.12 ± 1.13) withp= 0.00003 (p< 0.05). At 2 months post NSPT median SOD level (1.00) had come close to median SOD value of control group (0.85);p= 0.99 (p> 0.05). The resolution of inflammation with successful NSPT resulted in decreased SOD levels as in control group. Clinical parameters in patients with chronic periodontitis showed a significant improvement 2 months post NSPT (p< 0.05).CONCLUSION: Non-surgical periodontal therapy significantly improves the clinical parameters and restores previously increased SOD levels to normal in chronic periodontitis patients.

scholarly journals The Role of Prediabetes as a Predictive Factor for the Outcomes in Patients with STEMI. Which Is the Right Range of Glycated Hemoglobin to Adopt in This Setting?

2021 ◽  
Vol 11 (12) ◽  
pp. 5518
Author(s):  
Kyriazoula Chatzianagnostou ◽  
Letizia Guiducci ◽  
Umberto Paradossi ◽  
Alberto Ranieri De Caterina ◽  
Annamaria Mazzone ◽  
...  
Keyword(s):  
Risk Factors ◽  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Risk Factor ◽  
Prognostic Value ◽  
Glycated Hemoglobin ◽  
Who Guidelines ◽  
One Year ◽  
The Right

Background: Prediabetes (preT2D) is considered a subtle adverse cardiovascular (CV) risk factor after acute myocardial infarction. Glycated hemoglobin (HbA1c) ranges to identify preT2D are different between ADA and WHO guidelines (5.7–6.4 vs. 6.0–6.4%, respectively). Aim: To evaluate the prognostic value of HbA1c different preT2D-ranges and their correlation with demographic, instrumental, and laboratory parameters in STEMI. Methods: A total of 1681 patients (mean age 67 ± 13 years; 1217 males) were enrolled. Admission HbA1c was used to identify patients with no-T2D (<5.7%), HbA1c 5.70–5.99%, and WHO-preT2D with HbA1c 6–6.49%, and T2D (HbA1c ≥ 6.5). Results: HbA1c 5.7–5.99, WHO-preT2D, and T2D progressively correlated with an increasing number of CV risk factors. However, only T2D, but not preT2D, was significantly associated with adverse prognosis (in-hospital and one-year death). Conclusions: PreT2D is correlated with CV risk factors, but not with adverse prognosis as compared to no-T2D. Nonetheless, routine HbA1c testing in the STEMI population and HbA1c-5.7–5.99 patient inclusion in the preT2D category may help to identify those who may benefit from intervention and lifestyle strategies to early prevent preT2D progression.

scholarly journals The Prevalence of Hand Eczema and Its Determinants Among Female Hairdressers: A Cross-Sectional Survey

2018 ◽  
Vol 11 (1) ◽  
pp. 170-176
Author(s):  
Mohammad Hajaghazadeh ◽  
Abbas Jafari ◽  
Shole Jafari ◽  
Shirin Hekmatirad ◽  
Alireza Didarloo
Keyword(s):  
Risk Factors ◽  
Logistic Regression ◽  
General Population ◽  
Work Experience ◽  
Smoking Habit ◽  
Hand Eczema ◽  
Control Group ◽  
Cross Sectional ◽  
One Year ◽  
And Control

Introduction:Female hairdressers are exposed to various chemicals in their occupation which may lead to skin problems such as hand eczema.This study aimed to determine the one-year prevalence of hand eczema and its risk factors in a sample of Northwest Iranian female hairdressers in 2015.Methods:This study was a cross-sectional investigation in which 385 female hairdressers and 385 women from general population were selected as case and control, respectively.The prevalence of one-year hand eczema was obtained by Nordic occupationl skin questionnaireusing face-to-face interview. Data were analyzed using descriptive and inferential statistics (chi-square, independent t-test and logistic regression in SPSS software version 19).Results:The mean and standard deviation of age and work experience of hairdressers were 33.16 ± 7.2 and 8.72 ± 5.7 years, respectively.The prevalence of one-year hand eczema in hairdressers and control group were 27.8% (95%CI=23.22 -32.22) and 13.2% (95%CI=9.02-15.58), respectively. The Odds Ratio (OR) for hand eczema in the hairdressers was 2.52 (95%CI=1.72-3.64), compared to the control group as the reference. Using logistic regression analysis smoking habit [OR=3.44 (95%CI=1.73-6.85)],age less than 30 years [OR=1.76 (95%CI=1.04-2.96)] and working experience less than10 years [OR=3.14 (95%CI=1.63-6.04)]were independent risk factors for reporting one-year prevalence of hand eczemaConclusion:Female hairdressers were more likely to be at risk of developing hand eczema compared to general population. Smoking habit, younger age, and less work experience were the significant risk factors of hand eczema in hairdressers. Occupational health interventions should consider these factors in the control of hand eczema in hairdressers.

scholarly journals Salivary Interleukin-1 Levels in Chronic Periodontitis Patients after use of Nigella Sativa (Kalonji) Oil

2020 ◽  
Vol 29 (04) ◽  
pp. 205-210
Author(s):  
Ghazala Hassan ◽  
◽  
Sarah Ghafoor ◽  
Saima Chaudhry ◽  
Zubair Ahmed Khan
Keyword(s):  
Normal Saline ◽  
Chronic Periodontitis ◽  
Nigella Sativa ◽  
Interleukin 1 ◽  
Periodontal Pocket ◽  
Control Group ◽  
Statistical Interpretation ◽  
Pocket Depth ◽  
Nigella Sativa Oil ◽  
Periodontal Inflammation

OBJECTIVE: Periodontitis is the second most prevalent microbiome associated inflammatory disease posing a threat to oral health. Nigella sativa (Kalonji) has been used since ancient times as a remedy for oral inflammatory conditions. Interleukin1 (IL-1 ) is critical for periodontal inflammation, collagen degradation and bone turn over. The motive of our study was to determine the change in the levels of salivary IL-1 after the use of Nigella sativa oil to determine if it has any correlation with salivary IL-1 . METHODOLOGY: A parallel-arm triple-blind placebo-based randomized control trial was conducted on a total of ninety three patients. Out of these, fifty individuals with chronic periodontitis were included in the study as per the eligibility criteria. Baseline screening of the participants was done via clinical periodontal parameters such as periodontal pocket depth (PPD), clinical attachment loss (CAL), plaque index (PI) and bleeding on probing (BoP). These individuals were categorized into two groups; 1. Control Group (n=25), which was given normal saline as placebo; 2. Treatment Group, which was given Nigella sativa oil (n=25). All participants underwent scaling and root planing before the start of the trial. The intervention was given for two weeks. Salivary samples were collected on day 0 and day 15 and were evaluated for interleukin-1 levels using ELISA. The statistical interpretation was done using IBM SPSS (version 25.0, SPSS Inc.) on forty participants due to loss to follow up. RESULTS: Levels of salivary interleukin-1 came out to be statistically insignificant after two-week use of Nigella sativa oil. CONCLUSION: No correlation was found between the salivary IL-1 and the use of Nigella sativa oil in patients with chronic periodontitis in contrast to the patients using normal saline. KEYWORDS: Chronic periodontitis, Interleukin-1 , Nigella sativa, Kalonji, Salivary HOW TO CITE: Hassan G, Ghafoor S, Chaudhry S, Khan ZA. Salivary interleukin-1 Levels in chronic periodontitis patients after use of nigella sativa (Kalonji) oil. J Pak Dent Assoc 2020;29(4):205-210.

scholarly journals The effect of carbonate apatite membrane gelatin application on IL-1β level gingival crevicular fluid in chronic periodontitis patients

2019 ◽  
Vol 31 (3) ◽  
pp. 184
Author(s):  
Indra Mustika Setia Pribadi ◽  
Ina Hendiani ◽  
Reynaldy Sartiono
Keyword(s):  
Chronic Periodontitis ◽  
Gingival Crevicular Fluid ◽  
Test Group ◽  
Wilcoxon Test ◽  
Periodontal Pocket ◽  
Control Group ◽  
Carbonate Apatite ◽  
Scaling And Root Planing ◽  
Root Planing ◽  
Crevicular Fluid

Introduction: Apatite carbonate material plays an important role in bone tissue regeneration. The use of this membrane is expected to achieve better treatment success than those without additional therapy. The antimicrobial content in the apatite carbonate membrane can be used to support periodontal treatment of chronic periodontitis after scaling and root planing. The purpose of this research was to analyse the effect of carbonate apatite membrane gelatin application on the IL-1β level of the gingival crevicular fluid in chronic periodontitis patients. Methods: This research was a double-blind, randomised controlled trial method, with purposive sampling, and split-mouth design. The parameter was the IL-1β level in the gingival crevicular fluid before and after scaling and root planing. Carbonate apatite membrane gelatin was applied to the periodontal pocket on the test group after scaling and root planing. Data were analysed by the Wilcoxon test with a p-value < 0.05. Results: The reduction of IL-1β level gingival crevicular fluid on day-0 and day-30 on both groups showed significant value (p < 0.001). However, it showed no significant differences statistically, between the test group and the control group. Conclusion: Carbonate apatite membrane gelatin application might reduce the IL-1β level of the gingival crevicular fluid, but not showing a better reduction from the control group.Keyword: Carbonate apatite membrane gelatin, IL-1β, periodontal therapy

scholarly journals Diagnostic and Prognostic Value of TAT, PIC, TM, and t-PAIC in Malignant Tumor Patients With Venous Thrombosis

2020 ◽  
Vol 26 ◽  
pp. 107602962097104
Author(s):  
Kun Zhou ◽  
Jun Zhang ◽  
Zun-Rong Zheng ◽  
Yu-Zhen Zhou ◽  
Xun Zhou ◽  
...  
Keyword(s):  
Prognostic Value ◽  
Cox Regression ◽  
Malignant Tumors ◽  
Plasminogen Activator Inhibitor ◽  
Roc Curves ◽  
Prognostic Indicators ◽  
Control Group ◽  
Diagnostic Efficiency ◽  
Diagnostic And Prognostic Value ◽  
D Dimer

Background: Venous thromboembolism (VTE) is an important complication in patients with malignant tumors. Its exact diagnosis and treatment are still lacking. We used a high-sensitive chemiluminescence method to detect thrombin–antithrombin III complex (TAT), plasmin-α2-plasmininhibitor complex (PIC), thrombomodulin (TM), and tissue plasminogen activator–inhibitor complex(t-PAIC) in combination with D-dimer and fibrin degradation product (FDP) to analyze their diagnostic and prognostic value in patients with malignant tumors. Methods: In total, 870 patients with confirmed malignant tumors were included, 82 of whom had diagnosed VTE; 200 healthy individuals were classified as the control group. The TAT, PIC, TM, and t-PAIC were detected using Sysmex HISCL5000 automated analyzers, whereas FDP and D-dimer were detected using Sysmex CS5100 coagulation analyzer. Receiver operating characteristic (ROC) curves were used to evaluate the diagnostic efficiency. Survival probabilities were determined using Kaplan–Meier analysis, and multivariate analyses were performed using a Cox regression model. Results: Compared with healthy controls, patients with malignant tumors showed significantly elevated TAT, PIC, TM, t-PAIC, D-dimer, and FDP. Similarly, compared with patients in the non-thrombosis group, those in the thrombosis group showed significantly elevated levels of the above mentioned markers. Logistic regression analysis showed that TAT, PIC, TM, t-PAIC, D-Dimer, and FDP were all associated with VTE. ROC analysis showed that “TAT+PIC+TM+t-PAIC+D-dimer+FDP”showed the highest sensitivity and specificity. Patients with elevated TAT, PIC, TM, and t-PAIC had a significantly shorter survival. Multivariate Cox survival analysis showed that TM and t-PAIC were significantly associated with poor prognosis. In addition, the incidence of VTE was significantly lower in patients with malignant tumors who were treated with low-molecular-weight heparin (LMWH), and their survival period was significantly longer than that of patients with malignant tumors who were not treated with LMWH. Conclusion: TAT, PIC, TM, and t-PAIC combined with D-dimer and FDP were better than the application of a single marker in the diagnosis of VTE in patients with malignant tumors. TAT and PIC can be used as sensitive markers in the diagnosis of VTE but not as prognostic markers. TM and t-PAIC might be independent prognostic indicators in patients with malignant tumors, regardless of the state of thrombus.

scholarly journals Identification of Periopathogenes from Dental Plaque in Periodontal Patients with PCR Technique and Their Association with Composite Interleukin-1 Genotype

PRILOZI ◽  
2019 ◽  
Vol 40 (2) ◽  
pp. 89-97
Author(s):  
Aneta Atanasovska Stojanovska ◽  
Saska Todoroska ◽  
Mirjana Popovska ◽  
Ilijana Muratovska ◽  
Linda Zendeli Bedzeti
Keyword(s):  
Chronic Periodontitis ◽  
Dental Plaque ◽  
Bacterial Species ◽  
Interleukin 1 ◽  
Strong Association ◽  
Periodontal Pocket ◽  
Control Group ◽  
Pocket Depth ◽  
Significant Difference ◽  
Healthy Control

Abstract Introduction: The present study aimed to assess the presence of main types of microorganisms involved in the aetiopathogenesis of chronic periodontitis with PCR technique and determinates the presence of composite IL-1 genotype and their associations with founded bacteria. Material and method: The examined group was consisted from 20 subjects with diagnosed chronic periodontitis and 20 healthy control without periodontitis. Clinical parameters like gingival index (GI), plaque index (PI), bleeding on probing (BOP), periodontal pocket depth (PPD) and clinical attachment lost (CAL) were determinates. Subgingival dental plaque was collected using a sterilized paper point. We used Parodontose Plus test, reverse hybridization kit, for the detection of periodontal marker bacteria, as well as for the detection of composite Interleukin -1 Genotype Results: The most present bacterial species detected from subgingival dental plaque was Treponema denticola and Porfiromonas gingivalis which was present in 65% of examined patients. In relation to the presence of positive genotype in patients, there was no significant difference between the test and control group for p> 0.05 (p = 1.00). For χ2=8,17 (p=0,06, p<0,05) there is an association between Prevotella intermedia, and composite genotype. Between positive genotype and analyzed bacterial species A. actinomycetem comitans for p> 0.05 (p = 1.00), P. gingivalis for p> 0.05 (p = 0.16), T. Forsythia for p> 0.05 (p = 0.20), T. Denticola for p> 0.05 (p = 0.64) no association was found. Conclusion. This investigations confirmed the strong association of these five examined periopathogenes with periodontitis.

scholarly journals RISK FACTORS FOR MULTIPLE SCLEROSIS IN VOLYN REGION (UKRAINE)

2016 ◽  
Vol 6 ◽  
pp. 45-51
Author(s):  
Nataliia Bobryk ◽  
Larysa Sokolova
Keyword(s):  
Risk Factors ◽  
Multiple Sclerosis ◽  
Paternal Age ◽  
Control Group ◽  
Number Of Patients ◽  
History Of ◽  
One Year ◽  
Associated Risk Factors ◽  
Simplex Virus

The prevalence rate of multiple sclerosis (MS) in Volyn Region (101.0 per 100000) is the highest in Ukraine. To study MS risk factors in Volyn Region, special questionnaires were distributed among all MS patients residing in Volyn region. Results were obtained from 227 respondents including 154 women and 73 men of mean age 43±10.6 years. The control group included 105 healthy respondents inhabiting Volyn region. We found associated risk factors for MS to be: mother of Volyn origin, maternal age after 27 years old and paternal age after 29 years old at birth of respondent, subject's born as a third child, brestfeeding for less than one year, living in the zone of industrial pollution, near mobile, TV- and radio re-translators, full traffic automobile roads, time spending outdoors less than one hour in winter and less than eight hours in summer, consumption of fruit and vegetables less than 5 times a week, of beef less than 3 times a week, poultry meet less than 3, berries less than 3, cereals less than 4 times a week, chronic stressful situations in life. Patients with MS more frequently reported AVRI, hepatitis and herpes simplex virus. Among patients with MS there were fewer respondents with history of chickenpox, rubella and DPT (diphtheria, pertussis, tetanus), BCG immunizations. Here, we introduced a novel study of MS risk factors within Volyn Region. The Registry established in 2012 is being constantly updated and can be a database for a long-term retrospective study involving a large number of patients.

scholarly journals High Expression of VAV Gene Family Predicts Poor Prognosis of Acute Myeloid Leukemia

2021 ◽  
Vol 20 ◽  
pp. 153303382110658
Author(s):  
Dan Mu ◽  
Sili Long ◽  
Ling Guo ◽  
Wenjun Liu
Keyword(s):  
Acute Myeloid Leukemia ◽  
Western Blotting ◽  
Myeloid Leukemia ◽  
Poor Prognosis ◽  
Prognostic Value ◽  
High Expression ◽  
Control Group ◽  
Poor Overall Survival ◽  
Expression Levels ◽  
Acute Myeloid

Objectives: VAV family genes ( VAV1, VAV2, and VAV3) are associated with prognosis in various cancers; however, they have not been evaluated in acute myeloid leukemia (AML). In this study, the prognostic value of VAV expression in AML was evaluated by a single-center study in combination with bioinformatics analyses. Methods: The expression and prognostic value of VAVs in patients with AML were investigated using various databases, including GEPIA, CCLE, EMBL-EBI, UALCAN, cBioPortal, STRING, and DAVID. Blood samples from 35 patients with AML (non-M3 subtype) and 13 benigh individuals were collected at our center. VAV expression levels were detected by real-time quantitative PCR (RT-qPCR) and western blotting. Clinical data were derived from medical records. Results: Based on data from multiple databases, the expression levels of VAV1, VAV2, and VAV3 were significantly higher in AML than in control tissues ( P < 0.05). RT-qPCR and western blotting results showed that VAV expression in mRNA and protein levels were higher in patients with AML that in the control group ( P < 0.05). Complete remission rates were lower and risks were higher in patients with AML with high VAV1 expression than with low VAV1 expression ( P < 0.05). High levels of VAV2, VAV3, and VAV1 were related to a poor overall survival, and this relationship was significant for VAV1 ( P < 0.05). High expression levels of genes correlated with VAV1, such as SIPA1, SH2D3C, and HMHA1 were also related to a poor prognosis in AML. Functional and pathways enrichment analyses indicated that the contribution of the VAV family to AML may be mediated by the NF-κB, cAMP, and other pathways. Conclusion: VAVs were highly expressed in AML. In particular, VAV1 has prognostic value and is a promising therapeutic target for AML.

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