scholarly journals Comparison between the Antioxidant and Antidiabetic Activity of Fenugreek and Buckthorn in Streptozotocin-Induced Diabetic Male Rats

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Mohammed A. Alsieni ◽  
Haddad A. El Rabey ◽  
Abdulbasit I. Al-Sieni ◽  
Madeha N. Al-Seeni
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Density Lipoprotein ◽  
Negative Control ◽  
Seed Extract ◽  
Male Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Albino Rats ◽  
Leaf Aqueous Extract

This study is aimed at comparing the antidiabetic and antioxidant potential of fenugreek and buckthorn which are commonly used in modulating diabetes in the Middle East. In this study, the antioxidant and antidiabetic activity of the aqueous extracts of the leaf and seed of fenugreek and buckthorn was tested in streptozotocin-induced diabetic male rats fed with a fat-rich diet for 8 weeks. Thirty-six male albino rats were divided into 6 groups ( n = 6 ); the 1st group was the negative control. Diabetes was induced in the other 30 rats using streptozotocin, which were then divided into 5 groups; the 2nd was the untreated positive diabetic group, the 3rd was treated with fenugreek leaf aqueous extract, the 4th was treated with the fenugreek seed aqueous extract, the 5th was treated with buckthorn leaf aqueous extract, and the 6th was treated with buckthorn seed aqueous extract. The positive control group showed an increase in blood sugar, glycated hemoglobin, liver function enzymes, lactate dehydrogenase, kidney indices, total cholesterol, triglycerides, low- and very-low-density lipoprotein, immunoglobulins, and lipid peroxidation and a decrease in high-density lipoprotein, albumin, and antioxidant activity. The histology of the liver and testes showed severe histopathological alterations. Rats of groups 4-6 that were treated with the aqueous extract of the leaf and seed extract of fenugreek and buckthorn showed improvement of all biochemical and histopathological parameters. The seed extract of fenugreek and buckthorn showed more antioxidant activity than their leaves.

scholarly journals NORMOGLYCAEMIC EFFECTS OF AQUEOUS EXTRACT OF Parkia biglobosa LEAVES IN ALLOXAN-INDUCED DIABETIC RATS

2020 ◽  
Vol 5 (3) ◽  
pp. 23-39
Author(s):  
I. R. HASSAN, ◽  
A.A Adesokan ◽  
O.T. Odeyemi
Keyword(s):  
Aqueous Extract ◽  
Serum Proteins ◽  
Negative Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Albino Rats ◽  
Phytochemical Analysis ◽  
Parkia Biglobosa ◽  
Traditional Use

Background: Diabetes mellitus is a global health problem leading to an increase in the search for herbal normoglycaemic agents as alternative to the synthetic ones. Aqueous extract of Parkia biglobosa leaves was assessed for normoglycaemic effects in alloxan-induced diabetic rats. The study aim at providing scientific evidence to authenticate the traditional use of Parkia biglobosa leaves in the treatment of diabetes. Methodology: The plant was extracted using aqueous to obtain Parkia biglobosa Leaf Extract (PbLE), qualitative phytochemical analysis was determined using standard methods. Diabetes was induced in albino rats by intraperitoneal injection of 5% solution of alloxan (150 mg/kg bw). The rats were grouped into 5 groups (A, B, C, D and E) of 5 animals each. Group A consisted of non-diabetic rats which served as the control, Group B consisted of diabetic rats that were left untreated and served as negative control, Group C were given glucophage (reference) at a dose level of 7 mg/kg bw, Groups D and E were administered PbLE at the doses of 250 and 500 mg/kg bw respectively. Results: The glucose levels in the blood of rats were checked with a glucometer using the blood from the tail of the rats. Serum (proteins, lipid profiles, urea and creatinine), ALT, AST and ALP were all determined using standard procedures. The extract and the glucophage reduced the blood glucose level significantly (p < 0.05) from day 3 till the termination of the experiment.                                                                                                                                                Conclusion: Aqueous extract of Parkia biglobosa leaves possess antidiabetic activity and also the extract is relatively safe.  Hence the leaves of Parkia biglobosa can be explored in producing alternative antidiabetic drugs.

scholarly journals EFEK ANTIDIABETES EKSTRAK AIR KULIT BUAH PISANG AMBON (Musa paradisiaca L.) TERHADAP MENCIT (Mus musculus) MODEL HIPERGLIKEMIA

2015 ◽  
Vol 1 (2) ◽  
pp. 133-140
Author(s):  
Sri Indrawati ◽  
Yuliet Yuliet ◽  
Ihwan Ihwan
Keyword(s):  
Antioxidant Activity ◽  
Effective Dose ◽  
Aqueous Extract ◽  
Positive Control ◽  
Negative Control ◽  
Antidiabetic Activity ◽  
Glucose Levels ◽  
Musa Paradisiaca ◽  
Blood Glucose Levels ◽  
Before And After

Pisang Ambon (Musa paradisiaca L.) is one type of bananas usually consumed by Indonesian people. Besides its flesh which has high nutrition, its peels also has antioxidant activity. Antioxidants has the ability to reduce oxidative damage in people’s body with diabetes mellitus. Therefore, this study aimed to determine the antioxidant activity of the aqueous  extract of Pisang Ambon peels and to determine it’s effective dose as an antidiabetic agent in hyperglycemic mice. This study used male mice which all have been intravenously induced with alloxan at a dose of 50 mg/kgBW. They were then divided into five groups. The first two groups got Na CMC 0.5% (negative control) and glibenclamide 0.65 mg/kgBW (positive control), while the other three got  the aqueous  extract of Pisang Ambon peels successively at doses of 400, 800, and 1200 mg/kgBW. The data were statistically analyzed using ANOVA (Analysis of Variance) at 95% confidence interval with parameter of blood glucose levels difference between before and after treatment. The results showed that the aqueous extract of Pisang Ambon peels had antidiabetic activity at an effective dose of 400 mg/kgBW in hyperglycemic mice which was comparable to glibenclamide

scholarly journals PROTECTIVE ROLE AND ANTIOXIDANT ACTIVITY OF AQUEOUS EXTRACT OF ROSMARINUS OFFICINALIS AGAINST TRICHLOROACETATE-INDUCED TOXICITY IN LIVER OF MALE RATS

2018 ◽  
Vol 11 (6) ◽  
pp. 420
Author(s):  
Medhat Mostafa Abozid ◽  
Hoda Ea Farid
Keyword(s):  
Aqueous Extract ◽  
Liver Damage ◽  
Protective Role ◽  
Rosmarinus Officinalis ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Gamma Glutamyl Transferase ◽  
Group I ◽  
Glutamyl Transferase

 Objective: The current study was designed to estimate the potential protective role of the aqueous extract of rosemary (AER) (Rosmarinus officinalis) against trichloroacetic acid (TCA)-created hepatotoxicity in male albino rats.Methods: Forty male albino rats were separated into four groups of ten: Group I served as control; Group II was given AER (200 mg/kg/day) by gavage; Group III received TCA at the dose 50 mg/kg/day, and Group V was treated with AER (200 mg/kg/day) and received TCA (50 mg/kg/day). The experiment was carried out for 2 months.Results: The toxicity of TCA for rats was revealed by an elevation in liver marker enzymes activities (gamma-glutamyl transferase [GGT], alkaline phosphatase [ALP], aspartate transaminase [AST], alanine aminotransferase [ALT]) and conjugated bilirubin (CB) level, and a decrease in albumin and total protein (TP) levels. The TCA administration also caused a significant increase in the activities of catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD), and also malondialdehyde (MDA) level in liver tissues. These biochemical effects were accompanied by histological indicators of liver damage. Treatment with ARE recovered the liver damage instigated by TCA, as showed by perfection of liver enzyme markers (GGT, ALT, AST, ALP), CB, TP and albumin; as well as antioxidant parameters (CAT, SOD, GPx) and lipid peroxidation (MDA) and amelioration of histopathology changes in the liver tissues.Conclusion: It could be concluded that AER supplementation for 2 months in TCA-induced toxicity in rats benefited hepatic antioxidant status and improved liver injury and damage in male albino rats exposed to TCA.

The Effect of Different Extracts of Beetroots as Antioxidant and Anti-Anaemia On Phenylhydrazine-Induced Rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Ni Putu Ermi Hikmawanti ◽  
Lusi Putri Dwita ◽  
Dimas W. Wisnunanda ◽  
Fanny Farista
Keyword(s):  
Antioxidant Activity ◽  
Normal Control ◽  
Antioxidant Activities ◽  
Negative Control Group ◽  
Negative Control ◽  
Pharmaceutical Product ◽  
Normal Control Group ◽  
Male Rats ◽  
Control Group ◽  
Hydroethanolic Extract

Abstract Aim evaluate antioxidant and anti-anaemia activity of dichloromethane, hydroethanolic, and alkaloids-free hydroethanolic extracts of beetroot (Beta vulgaris (L.) subsp. vulgaris) on phenylhydrazine-induced rats. Methods Male rats were divided into five groups: normal control group, negative control group, dichloromethane extract group, hydroethanolic extract group, and alkaloids-free hydroethanolic extract group. All groups were induced with phenylhydrazine (30 mg.Kg−1 BW) for three days, except for the normal control group. After induction, each treatment group received each extract (200 mg.Kg−1 BW) for 21 days. The haematology parameters (haemoglobin levels, the number of erythrocytes, and haematocrit levels) were measured using Haematology Analyzer, and the antioxidant activity was measured through MDA level parameters in rats. Data were analysed using one-way ANOVA and then continued with the Tukey test. Results The results showed that the hydroethanolic extract of beetroot increased the percentage of erythrocytes (33.5%), haemoglobin (25%), and haematocrit (24.4%) to the negative control group, which was comparable to the normal control group (p > 0.05). In addition, the best antioxidant activity was shown in the hydroethanolic extract of beetroot, which is comparable to the normal group (p > 0.05). Conclusion The beetroot hydroethanolic crude extract could be potentially produced in a natural pharmaceutical product as a beneficial resource within anti-anaemia and antioxidant activities.

scholarly journals Aqueous Extract ofPhyllanthus niruriLeaves DisplaysIn VitroAntioxidant Activity and Prevents the Elevation of Oxidative Stress in the Kidney of Streptozotocin-Induced Diabetic Male Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Nelli Giribabu ◽  
Pasupuleti Visweswara Rao ◽  
Korla Praveen Kumar ◽  
Sekaran Muniandy ◽  
Somesula Swapna Rekha ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Rat Kidney ◽  
Male Rats ◽  
Diabetic Rat ◽  
Activity Levels ◽  
Leaf Aqueous Extract ◽  
Gpx Activity

P. nirurihas been reported to possess antidiabetic and kidney protective effects. In the present study, the phytochemical constituents andin vitroantioxidant activity ofP. nirurileaf aqueous extract were investigated together with its effect on oxidative stress and antioxidant enzymes levels in diabetic rat kidney.Results. Treatment of diabetic male rats withP. nirurileaf aqueous extract (200 and 400 mg/kg) for 28 consecutive days prevents the increase in the amount of lipid peroxidation (LPO) product, malondialdehyde (MDA), and the diminution of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activity levels in the kidney of diabetic rats. The amount of LPO showed strong negative correlation with SOD, CAT, and GPx activity levels.P. nirurileaf aqueous extract exhibitsin vitroantioxidant activity with IC50slightly lower than ascorbic acid. Phytochemical screening of plant extract indicates the presence of polyphenols.Conclusion.P. nirurileaf extract protects the kidney from oxidative stress induced by diabetes.

scholarly journals Antidiabetic and Antioxidant Activities of the Twigs of Andrograhis paniculata on Streptozotocin-Induced Diabetic Male Rats

BioChem ◽  
2021 ◽  
Vol 1 (3) ◽  
pp. 238-249
Author(s):  
Olubanke O. Ogunlana ◽  
Babatunde O. Adetuyi ◽  
Elohor F. Esalomi ◽  
Miracle I. Rotimi ◽  
Jacob O. Popoola ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Antioxidant Activities ◽  
Fasting Blood Glucose ◽  
Male Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Albino Rats ◽  
Oral Glucose ◽  
Distilled Water ◽  
Group 2

Background: Diabetes is associated with chronic hyperglycaemia, long-term damage, dysfunction, and organ failure. This study aims to evaluate the antidiabetic activity of the twigs of Andrographis paniculata and its toxicological markers on Streptozotocin (STZ)-induced diabetic Albino rats. Methods: A total of thirty rats were randomly divided into five groups of six animals each. Non-diabetic animals were treated with distilled water as non-diabetic sham control group 1, while diabetic animals (group 2, 3, 4 and 5) were treated with 60 mg/kg bw STZ intravenous (iv) and 100 mg/kg body weight (bwt) of metformin orally for group 2, distilled water for group 3, and 250 and 500 mg/kg bwt of Andrograhis paniculata (A. paniculata) for groups 4 and 5, respectively. The animals were dosed for 28 days, after which they were sacrificed. Liver and kidney function tests as well as livid profile tests were used as the biomarkers of toxicological assessment. Fasting blood glucose was carried out weekly. Oral Glucose Tolerance Test (OGTT) was conducted on the 28th day of the antidiabetic assessment. Results: A. paniculata groups 4 and 5 were significant at different doses (p < 0.05) in reducing the blood glucose level in comparison with metformin. There were significant changes in total and direct bilirubin, total protein, potassium, triglyceride and inorganic phosphorus in 500 mg/kg bwt of the treated group in comparison with the metformin and diabetic group groups. A. paniculata at 500 mg/kg bwt is most effective for its antidiabetic and organ protecting effects.

scholarly journals The Antidiabetic Activity ofNigella sativaand Propolis on Streptozotocin-Induced Diabetes and Diabetic Nephropathy in Male Rats

2017 ◽  
Vol 2017 ◽  
pp. 1-14 ◽  
Author(s):  
Haddad A. El Rabey ◽  
Madeha N. Al-Seeni ◽  
Amal S. Bakhashwain
Keyword(s):  
Diabetic Nephropathy ◽  
Methanol Extract ◽  
Nigella Sativa ◽  
Lipid Peroxide ◽  
Negative Control ◽  
Male Rats ◽  
Antidiabetic Activity ◽  
Albino Rats ◽  
Standard Diet ◽  
The Third

This study was conducted to compare the ameliorative effect ofNigella sativaand propolis methanol extract onstreptozotocin-induced diabetic male rats and treating diabetic nephropathy. Forty male Albino rats were divided into four groups; the first group was the negative control fed standard diet. The other 30 rats were injected withstreptozotocin to inducediabetes by a single intravenous injection and then divided equally into three groups; the second group was the positive diabetic control; the third and the fourth groups were treated orally with 20% w/wNigella sativaseeds methanol extract and propolis methanol extract (20% w/w), respectively. The rats of the second group showed increased glucose levels and lipid peroxide accompanied with reduction in superoxide dismutase, catalase, and glutathione-S-transferase enzyme activities compared with the negative control. Carboxymethyl lysine, interleukin-6, and immunoglobulins were also increased as a result of diabetes. Kidney function parameters were also elevated, while potassium and sodium levels were decreased. Moreover, tissues of kidney and pancreas showed severe histopathological changes. Treating the diabetic rats withNigella sativaand propolis methanol extract in the third and fourth groups, respectively, ameliorated all altered biochemical and pathological examinations approaching the negative control. Propolis was more effective thanNigella sativa.

scholarly journals ANTIDIABETIC ACTIVITY OF SENNA SURATTENSIS IN ALLOXAN-INDUCED DIABETIC RATS

2018 ◽  
Vol 11 (4) ◽  
pp. 160 ◽  
Author(s):  
Ellappan Thilagam ◽  
Kumarappan Chidambaram ◽  
Subhash Chandra Mandal
Keyword(s):  
Total Protein ◽  
Density Lipoprotein ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Serum Biomarkers ◽  
Lipoprotein Cholesterol ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Albino Rats ◽  
Present System

 Objective: Senna surattensis is a shrub plant which has been known for its diverse biological and pharmacological properties. This study is aimed to evaluate the antidiabetic activity of ethanolic extracts of S. surattensis (EESS) leaves in alloxan-induced diabetic rats.Methods: Experimental diabetes was induced by injection of a single dose of alloxan (120 mg/kg, intraperitoneal). Adult male Wistar albino rats were divided into five groups; normal control, diabetic control, diabetic EESS (200 mg/kg body weight (bw), diabetic EESS (400 mg/kg bw), and diabetic glibenclamide (5 mg/kg bw). Extracts were treated concurrently for 21 days. Blood samples were collected and centrifuged for estimation of fasting blood glucose (FBG), bw, serum biomarkers, lipid profile, total protein, albumin, and glycosylated hemoglobin (HbA1C) contents.Results: The increase in FBG, bw, liver biomarkers serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, alkaline phosphatase, free fatty acid, phospholipids (PL), triglycerides, total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, total protein, albumin, and HbA1C content were recorded in diabetic control rats. Daily oral administration of EESS treatment significantly (p<0.01) reverted the levels of serum biomarkers and enzymes activities to near normal values. A similar reduction was produced in FBG after 21 days of extract administration which compared significantly (p<0.01) with the control group and glibenclamide treated groups.Conclusion: The results suggest that EESS has anti-diabetic activity in diabetic rats, thereby justifying its traditional claim and augmenting it into the present system of medicine.

scholarly journals Comparison between the Hypolipidemic Activity of Parsley and Carob in Hypercholesterolemic Male Rats

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Haddad A. El Rabey ◽  
Madeha N. Al-Seeni ◽  
Habibah B. Al-Ghamdi
Keyword(s):  
Methanol Extract ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Male Rats ◽  
High Density Lipoproteins ◽  
Control Group ◽  
Albino Rats ◽  
Low Density ◽  
Creatine Kinase Mb ◽  
Liver Tissues

Hypercholesterolemia is commonly associated with obesity that leads to heart diseases and diabetes. The hepatocardioprotective activity of parsley and carob methanol extract was tested in hypercholesterolemic male rats. Twenty-four male albino rats were divided into four groups (n=6). Group 1 was the negative control group fed with fat rich diet, group 2 (G2) was hypercholesterolemic rats fed with fat rich diet with 2% cholesterol, and group 3 and group 4 (G3 and G4) were hypercholesterolemic rats supplemented with 2% cholesterol and cotreated with 20% w/w parsley seed methanol extract and 20% w/w carob legume methanol extract, respectively. The experiment was conducted for eight weeks. The positive hypercholesterolemic rats showed significant increase in serum levels of total cholesterol, triglycerides, low density lipoprotein (LDL), very low density lipoprotein (VLDL), lactate dehydrogenase (LDH), creatine kinase-mb, liver function enzymes, and decrease in the high density lipoproteins (HDL). Moreover, heart and liver tissues were ameliorated and nearly restored their normal appearance. It could be concluded that both parsley and carob extracts supplementations have a protective effect against hyperlipidemia and improved the histological alteration in heart and liver tissues. The methanol extract of parsley appeared to be more efficient than that of carob in lowering hypercholesterolemia.

scholarly journals Green coffee methanolic extract and silymarin protect against CCl4-induced hepatotoxicity in albino male rats

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Haddad A. El Rabey ◽  
Samar M. Rezk ◽  
Mohamed I. Sakran ◽  
Ghena M. Mohammed ◽  
Omar Bahattab ◽  
...  
Keyword(s):  
Olive Oil ◽  
Liver Toxicity ◽  
Methanolic Extract ◽  
Density Lipoprotein ◽  
Positive Control ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Green Coffee ◽  
Sprague Dawley

Abstract Background During the last few decades, patients worldwide have been interested in using alternative medicine in treating diseases to avoid the increased side effects of chemical medications. Green coffee is unroasted coffee seeds that have higher amounts of chlorogenic acid compared to roasted coffee. Green coffee was successfully used to protect against obesity, Alzheimer disease, high blood pressure and bacterial infection. Methods This study aimed to investigate the probable protective activity of the green coffee methanolic extract, silymarin and their combination on CCl4-induced liver toxicity in male rats. Thirty Sprague – Dawley male albino rats were divided into 5 groups; control negative (G1) just got the vehicle (olive oil) and the other four groups received CCl4 dissolved in olive oil through an intraperitoneal injection and were divided into untreated control positive group (G2), the third group (G3) was treated with green coffee methanolic extract, the fourth group (G4) was treated with silymarin, and the fifth group (G5) was treated with a combination of green coffee methanolic extract and silymarin. Results In the positive control group treated with CCl4 (G2), the CCl4-induced toxicity increased lipid peroxidation, IL-6, kidney function parameters, liver function enzymes, total cholesterol, triglycerides and low-density lipoproteins, and decreased irisin, antioxidants, CYP450 and high-density lipoprotein levels. Hepatic tissues were also injured. However, treating the injured rats in G3, G4 and G5 significantly improved the altered parameters and hepatic tissues. Conclusions Green coffee methanolic extract, silymarin, and their combination succeeded in protecting the male rats against CCl4 hepatotoxicity due to their antioxidant activity. Effect of green coffee methanolic extract mixed with silymarin in G5 was more efficient than that of green coffee methanolic extract in G3 or silymarin in G4.

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