scholarly journals Discovery of a Novel Long Noncoding RNA Lx8-SINE B2 as a Marker of Pluripotency

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Fuquan Chen ◽  
Miao Zhang ◽  
Xiao Feng ◽  
Xiaomin Li ◽  
Haotian Sun ◽  
...  
Keyword(s):  
Stem Cells ◽  
Long Noncoding Rna ◽  
Noncoding Rna ◽  
Embryonic Stem ◽  
Specific Expression ◽  
Self Renewal ◽  
Transcription Regulators ◽  
Full Length Sequence ◽  
Rapid Amplification ◽  
Important Marker

Pluripotency and self-renewal of embryonic stem cells (ESCs) are marked by core transcription regulators such as Oct4, Sox2, and Nanog. Another important marker of pluripotency is the long noncoding RNA (lncRNA). Here, we ind that a novel long noncoding RNA (lncRNA) Lx8-SINE B2 is a marker of pluripotency. LncRNA Lx8-SINE B2 is enriched in ESCs and downregulated during ESC differentiation. By rapid amplification of cDNA ends, we identified the full-length sequence of lncRNA Lx8-SINE B2. We further showed that transposable elements at upstream of lncRNA Lx8-SINE B2 could drive the expression of lncRNA Lx8-SINE B2. Furthermore, ESC-specific expression of lncRNA Lx8-SINE B2 was driven by Oct4 and Sox2. In summary, we identified a novel marker lncRNA of ESCs, which is driven by core pluripotency regulators.

scholarly journals Long Noncoding RNA Moderates MicroRNA Activity to Maintain Self-Renewal in Embryonic Stem Cells

2017 ◽  
Vol 9 (1) ◽  
pp. 108-121 ◽  
Author(s):  
Keriayn N. Smith ◽  
Joshua Starmer ◽  
Sarah C. Miller ◽  
Praveen Sethupathy ◽  
Terry Magnuson
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Long Noncoding Rna ◽  
Noncoding Rna ◽  
Embryonic Stem ◽  
Self Renewal

scholarly journals Divergent transcription of long noncoding RNA/mRNA gene pairs in embryonic stem cells

2013 ◽  
Vol 110 (8) ◽  
pp. 2876-2881 ◽  
Author(s):  
A. A. Sigova ◽  
A. C. Mullen ◽  
B. Molinie ◽  
S. Gupta ◽  
D. A. Orlando ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Long Noncoding Rna ◽  
Noncoding Rna ◽  
Embryonic Stem ◽  
Gene Pairs ◽  
Mrna Gene ◽  
Divergent Transcription

scholarly journals The long noncoding RNA lncR492 inhibits neural differentiation of murine embryonic stem cells

PLoS ONE ◽  
2018 ◽  
Vol 13 (1) ◽  
pp. e0191682 ◽  
Author(s):  
Maria Winzi ◽  
Nuria Casas Vila ◽  
Maciej Paszkowski-Rogacz ◽  
Li Ding ◽  
Svenja Noack ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Long Noncoding Rna ◽  
Neural Differentiation ◽  
Noncoding Rna ◽  
Embryonic Stem ◽  
Murine Embryonic Stem Cells

Long noncoding RNA HOTAIR promotes the self-renewal of leukemia stem cells through epigenetic silencing of p15

2018 ◽  
Vol 67 ◽  
pp. 32-40.e3 ◽  
Author(s):  
Shenmeng Gao ◽  
Bin Zhou ◽  
Haiying Li ◽  
Xingzhou Huang ◽  
Yanfei Wu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Long Noncoding Rna ◽  
Noncoding Rna ◽  
Epigenetic Silencing ◽  
The Self ◽  
Leukemia Stem Cells ◽  
Self Renewal

scholarly journals Long noncoding RNA LYPLAL1-AS1 regulates adipogenic differentiation of human mesenchymal stem cells by targeting desmoplakin and inhibiting the Wnt/β-catenin pathway

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Yanlei Yang ◽  
Junfen Fan ◽  
Haoying Xu ◽  
Linyuan Fan ◽  
Luchan Deng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Long Noncoding Rna ◽  
Noncoding Rna ◽  
Adipogenic Differentiation ◽  
Noncoding Rnas ◽  
Human Mesenchymal Stem Cells ◽  
Human Mscs ◽  
Direct Target ◽  
Rapid Amplification

AbstractLong noncoding RNAs are crucial factors for modulating adipogenic differentiation, but only a few have been identified in humans. In the current study, we identified a previously unknown human long noncoding RNA, LYPLAL1-antisense RNA1 (LYPLAL1-AS1), which was dramatically upregulated during the adipogenic differentiation of human adipose-derived mesenchymal stem cells (hAMSCs). Based on 5′ and 3′ rapid amplification of cDNA ends assays, full-length LYPLAL1-AS1 was 523 nt. Knockdown of LYPLAL1-AS1 decreased the adipogenic differentiation of hAMSCs, whereas overexpression of LYPLAL1-AS1 enhanced this process. Desmoplakin (DSP) was identified as a direct target of LYPLAL1-AS1. Knockdown of DSP enhanced adipogenic differentiation and rescued the LYPLAL1-AS1 depletion-induced defect in adipogenic differentiation of hAMSCs. Further experiments showed that LYPLAL1-AS1 modulated DSP protein stability possibly via proteasome degradation, and the Wnt/β-catenin pathway was inhibited during adipogenic differentiation regulated by the LYPLAL1-AS1/DSP complex. Together, our work provides a new mechanism by which long noncoding RNA regulates adipogenic differentiation of human MSCs and suggests that LYPLAL1-AS1 may serve as a novel therapeutic target for preventing and combating diseases related to abnormal adipogenesis, such as obesity.

scholarly journals Telomeric noncoding RNA promotes mouse embryonic stem cell self-renewal through inhibition of TCF3 activity

2018 ◽  
Vol 314 (6) ◽  
pp. C712-C720 ◽  
Author(s):  
Xiaojuan Xu ◽  
Mengmeng Guo ◽  
Na Zhang ◽  
Shoudong Ye
Keyword(s):  
Stem Cells ◽  
Noncoding Rna ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Promoting Effect ◽  
Self Renewal ◽  
Downstream Target ◽  
Undifferentiated State ◽  
Fate Decision

Although long noncoding RNAs (lncRNAs) are emerging as new modulators in the fate decision of pluripotent stem cells, the functions of specific lncRNAs remain unclear. Here, we found that telomeric RNA (TERRA or TelRNA), one type of lncRNAs, is highly expressed in mouse embryonic stem cells (mESCs) but declines significantly upon differentiation. TERRA is induced by the Wnt/β-catenin signaling pathway and can reproduce its self-renewal-promoting effect when overexpressed. Further studies revealed that T cell factor 3 ( TCF3) is a potential downstream target of TERRA and mediates the effect of TERRA in mESC maintenance. TERRA inhibits TCF3 transcription, while enforced TCF3 expression abrogates the undifferentiated state of mESCs supported by TERRA. Accordingly, the transcripts of the pluripotency genes Esrrb, Tfcp2l1, and Klf2, repressed by TCF3 in mESCs, are increased in TERRA-overexpressing cells. Our study therefore highlights the important role of TERRA in mESC maintenance and also uncovers a mechanism by which TERRA promotes self-renewal. These data will expand our understanding of the pluripotent regulatory network of ESCs.

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