scholarly journals Magnitude of Aflatoxigenic Aspergillus Species, Level of Aflatoxin B1, and Associated Factors in Stored Feed at Poultry Farms in Dire Dawa, Ethiopia

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Ambachew Motbaynor ◽  
Dawit Kassaye ◽  
Migbaru Keffale ◽  
Pawlos Wasihun
Keyword(s):  
Aspergillus Flavus ◽  
Aflatoxin B1 ◽  
Sampling Technique ◽  
Aspergillus Species ◽  
Poultry Feed ◽  
Toxic Metabolite ◽  
Fungal Contamination ◽  
High Moisture ◽  
Cross Sectional ◽  
Dire Dawa

Aflatoxin, the secondary toxic metabolite of Aspergillus species, particularly aflatoxigenic Aspergillus flavus and parasiticus, has a detrimental effect on poultry health and production. There exists some information gap about the magnitudes of aflatoxigenic Aspergillus species and aflatoxin in poultry feeds in the study area. Thus, the study was conducted to estimate the magnitude and assess the related potential factors of aflatoxigenic Aspergillus species with evaluations of the level of aflatoxin B1 in stored poultry feed at selected farms in Dire Dawa, Ethiopia. A cross-sectional study design was carried out on 374 poultry feed samples recruited by using a stratified simple random sampling technique. A pretested structured questionnaire was used to assess the level of knowledge and prevention practices associated with aflatoxin in poultry feed. The isolation of aflatoxigenic Aspergillus species was made by Aspergillus flavus parasiticus media, and aflatoxin B1 was estimated by aflatoxin B1 enzyme-linked immune sorbent assay. Results showed that the magnitude of aflatoxigenic Aspergillus species was 72.5% (95% CI: 67.6–76.9). The odds at which the species isolated were higher ( p < 0.05 ) in feeds stored more than two months (AOR = 2.69), the presence of rodents in the storing room (AOR = 2.67), feeds having high moisture content (AOR = 1.5), and feed ingredient types (AOR = 4.3) compared to their counter parts. Only 34.4 and 32.8 percent of the respondents have better knowledge and apply prevention practice about fungal contamination and aflatoxin production in poultry feed, respectively. The occurrence of aflatoxigenic Aspergillus species in poultry feed was associated with the presence of rodents in the feed storing room with long storing period and high moisture contents of the feed. The knowledge and prevention practices employed by farm managers and workers about fungal contamination and aflatoxin in poultry feed are found low.

Availability of Adequately Iodized Salt at Household Level and Associated Factors in Dire Dawa, Eastern Ethiopia

2016 ◽  
Vol 5 (4) ◽  
pp. 392
Author(s):  
Anteneh Berhane Yaye ◽  
Negga Baraki ◽  
Birhanu Seyum Endale
Keyword(s):  
Iodine Deficiency ◽  
Associated Factors ◽  
Public Health Problem ◽  
Sampling Technique ◽  
Iodized Salt ◽  
School Age Children ◽  
Cross Sectional ◽  
Eastern Ethiopia ◽  
Household Level ◽  
Dire Dawa

In Ethiopia, Iodine Deficiency Disorder has been recognized as a serious public health problem for the past six decades. In 2011, an estimated <br /> 12 million school-age children were living with inadequate iodine, and <br /> 66 million people were at risk of iodine deficiency. One out of every <br /> 1000 people is a cretin mentally handicapped, due to a congenital thyroid deficiency, and about 50000 prenatal deaths are occurring annually due to iodine deficiency disorders. Only 5.7% of the households were using iodized salt in Dire Dawa city Administration, which is below the legal requirement.This study assessed availability of adequately iodized salt at household level and associated factors in Dire Dawa town, East Ethiopia. Community based cross-sectional study was carried out among households in Dire Dawa town during March 16-26, 2015. Multistage sampling technique was used. Data were collected using a pretested and structured questionnaire by a face-to-face interview technique. Bivariate and multivariate analyses were performed to check associations and control confounding. A total of 694 participants were participated. The availability of adequately iodized salt (≥15 parts per million) in the study area was 7.5% (95% CI; 5.6-9.5). Multivariate result showed that health information about iodized salt (AOR=8.96, 95% CI; 4.68-17.16) (p=0.03), good knowledge about iodized salt (AOR=9.23, 95% CI; 3.34-25.5) (p=0.01) and using packed salt (AOR=3.99, 95% CI; 1.48-10.73) (p=0.006) were associated with availability of adequately iodized salt at household level. Availability of adequately iodized salt at household level was very low.  Hence, households should be sensitized about importance of iodized salt and its proper handling at the household level.

scholarly journals Assessment of Knowledge and Attitude among Pharmacists toward Pharmaceutical Care in Eastern Ethiopia

2020 ◽  
Vol 2020 ◽  
pp. 1-7 ◽  
Author(s):  
Kirubel Minsamo Mishore ◽  
Abraham Nigussie Mekuria ◽  
Assefa Tola ◽  
Yohanes Ayele
Keyword(s):  
Pharmaceutical Care ◽  
Care Service ◽  
Development Program ◽  
Community Pharmacists ◽  
Sampling Technique ◽  
Community Pharmacies ◽  
Cross Sectional ◽  
Eastern Ethiopia ◽  
Dire Dawa ◽  
Knowledge And Attitude

Objective. To assess knowledge and attitudes toward pharmaceutical care service among hospital and community pharmacists working in Harar and Dire Dawa town, Eastern Ethiopia. Method. A descriptive cross-sectional study was conducted among pharmacists working in hospital and community pharmacies, 2018. A total of 43 health settings (6 hospital and 37 community pharmacies) were involved in this study. All pharmacists who met the inclusion criteria were selected using a purposive sampling technique to take part in the study. The pretested structured self-administered questionnaires were used to collect data. The collected data was coded, entered, and analyzed using Statistical Package for Social Sciences (SPSS) version 21.0. The findings were presented by frequencies and percentages, and summary measures were displayed using tables. Chi-Square test and Fisher’s exact test were performed to determine the association between sociodemographic characteristics and the level of knowledge and attitude about pharmaceutical care. The study protocol was approved by the Harar Health Sciences College Research Ethics Review Committee. Results. A total of seventy-eight pharmacists were included in the study with a response rate of 97.5%. The mean age (±Standard Deviation (SD)) of the study participants was 32.47 ± 7.42 years, and the majority (88.3%) of the respondents were males. 56.4% of the respondents were working in the hospitals while 43.6% were working in community pharmacy. Overall, 85.9% of the respondents had good knowledge of pharmaceutical care. The types of training curriculum of the participants showed an association with the attitude of pharmacists (P value = 0.022). Similarly, pharmacists’ knowledge was associated with their practice setting (P value = 0.008). Conclusion. The majority of pharmacists are knowledgeable about PC. However, nearly half of the pharmacists had an unfavorable attitude toward pharmaceutical care. Harari Regional and Dire Dawa City Health Bureaus should organize and provide in-service training on pharmaceutical care to pharmacists working in community and hospital pharmacies. Furthermore, the bureaus should advocate pharmaceutical care as one area in a continuous professional development program.

scholarly journals Harmful Effects and Control Strategies of Aflatoxin B1 Produced by Aspergillus flavus and Aspergillus parasiticus Strains on Poultry: Review

Toxins ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 176 ◽  
Author(s):  
Ahmed Fouad ◽  
Dong Ruan ◽  
HebatAllah El-Senousey ◽  
Wei Chen ◽  
Shouqun Jiang ◽  
...  
Keyword(s):  
Aspergillus Flavus ◽  
Aflatoxin B1 ◽  
Egg Production ◽  
Egg Quality ◽  
Aspergillus Parasiticus ◽  
Control Strategies ◽  
Antioxidant Defense System ◽  
Poultry Feed ◽  
Feed Conversion ◽  
Poultry Diets

The presence of aflatoxin B1 (AFB1) in poultry diets decreases the hatchability, hatchling weight, growth rate, meat and egg production, meat and egg quality, vaccination efficiency, as well as impairing the feed conversion ratio and increasing the susceptibility of birds to disease and mortality. AFB1 is transferred from poultry feed to eggs, meat, and other edible parts, representing a threat to the health of consumers because AFB1 is carcinogenic and implicated in human liver cancer. This review considers how AFB1 produced by Aspergillus flavus and Aspergillus parasiticus strains can affect the immune system, antioxidant defense system, digestive system, and reproductive system in poultry, as well as its effects on productivity and reproductive performance. Nutritional factors can offset the effects of AFB1 in poultry and, thus, it is necessary to identify and select suitable additives to address the problems caused by AFB1 in poultry.

Occurrence of Aflatoxin and Aflatoxigenic Molds in Foods and Feed in Spain

1986 ◽  
Vol 49 (6) ◽  
pp. 445-448 ◽  
Author(s):  
V. SANCHIS ◽  
N. SALA ◽  
A. PALOMES ◽  
P. SANTAMARINA ◽  
P. A. BURDASPAL
Keyword(s):  
Aspergillus Flavus ◽  
Aflatoxin B1 ◽  
Aflatoxin Contamination ◽  
Cereal Grains ◽  
Maize Flour ◽  
Fungal Contamination ◽  
Bread Making ◽  
Low Incidence ◽  
Aflatoxin B2 ◽  
Mixed Feeds

A survey was carried out to obtain data on the occurrence of aflatoxin and aflatoxigenic mold contamination of foods in Spain. A variety of commodities amounting to 338 samples were analyzed, comprising cereal grains, mixed feeds, edible nuts, wheat flour for bread-making, biscuits, sliced bread, soya beans and breakfast cereals. The results reveal a rather low incidence of aflatoxin contamination in samples tested. Aflatoxins were detected in 4 of 27 samples of mixed feeds at levels below 5 μg/kg; one sample of peanuts was contaminated with 120 μg aflatoxin B1/kg and 22 μg aflatoxin B2/kg. Aflatoxins B1 and B2 were also detected in a lot of whole maize flour, averaging 8 μg/kg and 3 μg/kg, respectively. Of a total of 288 samples tested, 100% showed variable incidences of fungal contamination. Maize samples were the ones most frequently contaminated with Aspergillus flavus (54.5%). Strains of A. flavus isolated from maize samples also showed the highest proportion of aflatoxigenic molds (17.2%) compared with those isolated from other sources.

scholarly journals Availability of Adequately Iodized Salt at Household Level and Associated Factors in Dire Dawa, Eastern Ethiopia

2016 ◽  
Vol 5 (4) ◽  
pp. 392
Author(s):  
Anteneh Berhane Yaye ◽  
Negga Baraki ◽  
Birhanu Seyum Endale
Keyword(s):  
Iodine Deficiency ◽  
Associated Factors ◽  
Public Health Problem ◽  
Sampling Technique ◽  
Iodized Salt ◽  
School Age Children ◽  
Cross Sectional ◽  
Eastern Ethiopia ◽  
Household Level ◽  
Dire Dawa

In Ethiopia, Iodine Deficiency Disorder has been recognized as a serious public health problem for the past six decades. In 2011, an estimated 12 million school-age children were living with inadequate iodine, and 66 million people were at risk of iodine deficiency. One out of every 1000 people is a cretin mentally handicapped, due to a congenital thyroid deficiency, and about 50000 prenatal deaths are occurring annually due to iodine deficiency disorders. Only 5.7% of the households were using iodized salt in Dire Dawa city Administration, which is below the legal requirement.This study assessed availability of adequately iodized salt at household level and associated factors in Dire Dawa town, East Ethiopia. Community based cross-sectional study was carried out among households in Dire Dawa town during March 16-26, 2015. Multistage sampling technique was used. Data were collected using a pretested and structured questionnaire by a face-to-face interview technique. Bivariate and multivariate analyses were performed to check associations and control confounding. A total of 694 participants were participated. The availability of adequately iodized salt (≥15 parts per million) in the study area was 7.5% (95% CI; 5.6-9.5). Multivariate result showed that health information about iodized salt (AOR=8.96, 95% CI; 4.68-17.16) (p=0.03), good knowledge about iodized salt (AOR=9.23, 95% CI; 3.34-25.5) (p=0.01) and using packed salt (AOR=3.99, 95% CI; 1.48-10.73) (p=0.006) were associated with availability of adequately iodized salt at household level. Availability of adequately iodized salt at household level was very low.  Hence, households should be sensitized about importance of iodized salt and its proper handling at the household level.

scholarly journals Study of some risk factors for fungal contamination of dental unit waterlines in Alexandria, Egypt

2021 ◽  
Vol 15 (08) ◽  
pp. 1197-1204
Author(s):  
Eman Abdel Hamid Omran ◽  
Aleya Abdel Gawad Abbass ◽  
Amani Farouk Abaza ◽  
Eman Moftah Elzouki
Keyword(s):  
Risk Factors ◽  
Aspergillus Flavus ◽  
Candida Dubliniensis ◽  
Control Measures ◽  
Potential Hazard ◽  
Sample Collection ◽  
Fungal Contamination ◽  
Candida Spp ◽  
Cross Sectional ◽  
Dental Unit Waterlines

Introduction: Monitoring the microbial quality of water in dental unit waterlines is an important part of infection control measures carried out in dental clinics. Fungal contamination of such waterlines has not been extensively studied, compared with bacterial contamination. This study aimed at assessing the magnitude and risk factors for fungal contamination of dental unit waterlines. Methodology: This cross-sectional study included 82 dental units, randomly collected from 3 private clinics and 8 governmental hospitals in Alexandria, Egypt. A total of 204 water samples from dental unit waterlines output were membrane-filtered and cultured for fungal enumeration and species identification. The biofilm forming-ability was assessed for the most prevalent fungal species. The acceptability of samples was determined according to the Swedish drinking water guidelines. Results: The acceptability of samples was 89.7%. The most common mould was Aspergillus flavus, while Candida spp. was the most common yeast (10 isolates), with unusual predominance of Candida dubliniensis (9 isolates). All isolates of Aspergillus flavus and Candida dubliniensis were biofilm-formers. The risk factors for fungal contamination of dental unit waterlines included: dental specialty (p = 0.042), time of sample collection (p < 0.001), older age of dental unit (p < 0.001) and use of 5-15% of sodium hypochlorite. Conclusions: The presence of biofilm-forming fungi in dental unit waterlines is a potential hazard, even when samples have acceptable levels of fungal counts. Risk factors for contamination are numerous and should be addressed.

scholarly journals Mycobiota and aflatoxin B1 in poultry feeds

2019 ◽  
Vol 35 (1) ◽  
pp. 61-69
Author(s):  
Vesna Krnjaja ◽  
Tanja Petrovic ◽  
Slavica Stankovic ◽  
Milos Lukic ◽  
Zdenka Skrbic ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Laying Hens ◽  
Aflatoxin Contamination ◽  
Poultry Feed ◽  
Assay Method ◽  
Plate Count ◽  
Natural Occurrence ◽  
Fungal Contamination ◽  
Penicillium Species ◽  
Feed Samples

In this study, a total of 30 poultry (chicken and laying hens) feed samples collected from different poultry farms in Serbia in 2016 were tested for fungal and aflatoxin contamination. Using the plate count and standard mycological methods, total fungal counts and potentially toxigenic fungal genera were determined. Natural occurrence of aflatoxin B1 (AFB1) was detected by ELISA (enzyme-linked immune sorbent assay) method. The total fungal count was in the range from 1 x 102 (2 log CFU g-1) to 1.83 x 105 CFU g-1 (5.26 log CFU g-1). The majority of the chicken feeds (78.57%) had the total fungal count in the ranged from 1 x 102 to 4.8 x 104 CFU g-1, whereas in 68.75% of the laying hens feeds it was ranged from 5.3 x 104 to 1.83 x 105 CFU g-1. In 21.43% of the chicken feeds fungal contamination reached the level above the regulation limits. Three potentially toxigenic fungal genera, Aspergillus, Fusarium, and Penicillium, have been identified. In the tested poultry feed samples, more samples contaminated with Aspergillus were determined compared to samples contaminated by Fusarium and Penicillium species. The AFB1 was detected in concentrations from 1.34 to 18.29?g kg-1, with an average of 4.47 and 4.56?g kg-1 in the chicken and laying hens feed samples, respectively. In 14.29% of the chicken feeds, the level of AFB1 was above the regulation limits. The obtained results confirmed the importance of continuous mycological and mycotoxicological control of poultry feed, as well as need to improve risk assessments of such contaminants along the food chain.

PERCEIVED EFFECTS OF STREET HAWKING ON THE WELL-BEING OF CHILDREN IN ANYIGBA, DEKINA LOCAL GOVERNMENT AREA OF KOGI STATE, NIGERIA

2018 ◽  
Vol 8 (1) ◽  
pp. 01-07
Author(s):  
Alfred Eboh
Keyword(s):  
Local Government ◽  
Survey Design ◽  
Sampling Technique ◽  
Well Being ◽  
Local Government Area ◽  
Negative Consequences ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Perceived Effects ◽  
The Government

Background: The hawking of wares by children has been a serious issue confronting the Nigerian society. Children hawk in some of the most horrible conditions conceivable, where they face a serious risk of injury, chronic illness, kidnapping, rape or death. Objective: The focus of this study was to assess the perceived effects of street hawking on the well-being of children in Anyigba, Dekina Local Government Area of Kogi State. Methods: The population of this study consists of parents of the street hawkers in Anyigba while cross-sectional survey design was used through the purposive sampling technique to choose the sample size of one hundred and sixty-two (162) respondents. The validated structured questionnaire and In-Depth Interviews (IDIs) served as the instruments for the data collection respectively. The hypotheses were tested using Chi-Square at a predetermined 0.05 level of significance. The quantitative data were analysed with the aid of the SPSS (version 20). Results: The results indicated among others that street hawking had significant social implications and physical consequences on children's moral behaviour as well as health status in the study area. Conclusion: The study, therefore, concluded that the government of Kogi State should carry out an enlightenment campaign through the media and religious institutions on the negative consequences of street hawking are recommended as panacea. Also, the child right act instrument and its implementation should be strengthened in order to curb street hawking in the study area.

Nurse’s knowledge and Awareness Regarding Cervical Cancer and its Prevention in Different Hospitals of Quetta, Pakistan

2017 ◽  
Vol 4 (1) ◽  
pp. 436-441
Author(s):  
Saba Haider ◽  
Noman Ul Haq ◽  
Sohail Riaz ◽  
Aqeel Nasim ◽  
Muhammad Saood ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Family History ◽  
Sampling Technique ◽  
Knowledge Score ◽  
Validity And Reliability ◽  
Contributing Factors ◽  
Cross Sectional ◽  
Adequate Knowledge ◽  
Current Area ◽  
One Year

Objective: This study aimed to assess the knowledge and awareness regarding cervical cancer and its prevention among nurses working in different hospitals of Quetta, Pakistan. Methodology: The cross sectional, descriptive study was conducted by using structured questionnaire in different hospitals of Quetta from January to September 2016. Convenient sampling technique was applied by targeting all the nurses working in different hospitals of Quetta city. Study questionnaire was developed and tested for validity and reliability. Descriptive and inferential statistics (Mann Whitney U test and Kruskal Wallis tests, p<0.05) were used to assess the significance among study variables and were performed by using IBM SPSS v.20. Results: Out of 415 distributed questionnaires 324 were returned (response rate of 78%). The mean Age of respondents was 28.18 ±9.5 years. Majority (n=127, 43.3%) of participants were interns and had no or less than one year of experience (n=128, 43.7%) with negative family history of any cancer (n=275, 93.9%). Mean knowledge score was 18.52±4.84with majority (n=258, 88.1%) had adequate knowledge regarding cervical cancer. Respondent sage, current area of practice, qualification, Institute of degree and past family history were contributing factors (p > 0.05) in adequate knowledge in this study. The results also reviled that not only 68.3% (n=200) and 65.5% (n=192) respondents knew that cervical cancer is vaccine preventable and availability of the vaccine for it. Conclusions: Nurses working in different hospitals of Quetta city had better understanding of the disease cervical cancer and its prevention. Yet many of the respondent are not aware of it vaccine and its availability.

ADOLESCENT BEHAVIOR IN UNWANTED PREGNANCY PREVENTION

2019 ◽  
Vol 1 (1) ◽  
pp. 41-46
Author(s):  
Arip Ambulan Panjaitan
Keyword(s):  
Sexual Behavior ◽  
Reproductive Health ◽  
Risky Sexual Behavior ◽  
Parental Education ◽  
Unwanted Pregnancy ◽  
Sampling Technique ◽  
Adolescent Behavior ◽  
Cluster Sampling ◽  
Chi Square ◽  
Cross Sectional

Introduction: Adolescent are at high risk of unwanted pragnency, including abortion, STIs, HIV/AIDS. Risky sexual behavior is one of the entrance transmissions of unwanted pregnancy. Such behavior can be influenced by various factors, beyond and within the individual factors. Teens need the support and motivation in deciding not to do risky sexual behavior. The purpose of this study was to investigate the determinants of adolescent behavior in the prevention of unwanted pregnancy.Methods: This research used cross-sectional design. The populations were students of senior high school in District Sintang. The were 123 respondents chosen by cluster sampling technique for this study. All data were collected using questionnaires and then analysed using bivariate (chi square) and multivariate analysis (logistic regression).Results: The results showed that the majority of respondents did not have good knowledge about the prevention of unwanted pregnancy. Related variable is the level of parental education (p=0.001), the support of parents/guardians (p=0.009), support teachers (p=0.005), peer support (p=0.039), residency (p=0.009), a pastime activity (p=0.000), knowledge of adolescents about reproductive health (p=0.016), perception ability of adolescents (p=0.006) and attitude of adolescents (p=0.049). Adolescent self-perception abilities are variables that most influence on the behavior of adolescents in the prevention of unwanted pregnancy.Conclusions: Efforts to improve reproductive health programs should be early and adolescent have responsibilities as well as healthy behaviors.

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