scholarly journals Green Synthesis of Selenium Nanoparticles by Cyanobacterium Spirulina platensis (abdf2224): Cultivation Condition Quality Controls

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Shohreh Alipour ◽  
Sara Kalari ◽  
Mohammad Hossein Morowvat ◽  
Zahra Sabahi ◽  
Ali Dehshahri
Keyword(s):  
Green Synthesis ◽  
Spirulina Platensis ◽  
Sodium Selenite ◽  
Antioxidant Activities ◽  
Light Exposure ◽  
Scale Up ◽  
Continuous Light ◽  
Selenium Nanoparticles ◽  
Cultivation Condition ◽  
Ft Ir

Selenium nanoparticles (SeNPs) are well-known bioactive compounds. Various chemical and biological methods have been applied to SeNP synthesis. Spirulina platensis is a widely used blue-green microalgae in various industries. In this study, the biosynthesis of SeNPs using sodium selenite and Spirulina platens has been developed. The SeNP synthesis was performed at different cultivation condition including pH and illumination schedule variation. The SeNPs were characterized by FT-IR, XRD, size, and zeta potential measurements, and the antioxidant activities of selected SeNPs were evaluated by DPPH and FRAP assays. FT-IR analysis showed the production of SeNPs. The 12 h dark/12 h light cycles and continuous light exposure at pH 5 led to the production of stable SeNPs with sizes of 145 ± 6 and 171 ± 13  nm, respectively. Antioxidant activity of selected SeNPs was higher than sodium selenite. It seems that green synthesis is a safe method to produce SeNPs as well as a convenient method to scale-up this production.

scholarly journals Production of exopolysaccharide by strains of Lactobacillus plantarum YO175 and OF101 isolated from traditional fermented cereal beverage

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5326 ◽  
Author(s):  
Adekemi Titilayo Adesulu-Dahunsi ◽  
Kumaraswamy Jeyaram ◽  
Abiodun Isiaka Sanni ◽  
Kolawole Banwo
Keyword(s):  
Lactobacillus Plantarum ◽  
Antioxidant Activities ◽  
Scale Up ◽  
Food Industries ◽  
Ft Ir ◽  
Experimental Values ◽  
Central Composite ◽  
Ft Ir Spectroscopy ◽  
Significant Factors

Lactobacillus plantarum YO175 and OF101 isolates from Nigerian traditional fermented cereal gruel ‘ogi’, were investigated on the basis of their capability to produce exopolysaccharide (EPS) on sucrose modified deMan Rogosa Sharpe medium (mMRS). Functional groups analysis of the EPSs produced (EPS-YO175 and EPS-OF101) by Fourier-transform infrared (FT-IR) spectroscopy revealed the presence of –OH, C=O and C–H groups. The chemical composition of EPS-YO175 and EPS-OF101 showed the presence of 87.1% and 80.62% carbohydrates and 1.21% and 1.47% protein. For maximum EPS yield, three significant factors were optimized using central composite design and response surface methodology, the predicted maximum EPS produced was 1.38 g/L and 2.19 g/L, while the experimental values were 1.36 g/L and 2.18 g/L for EPS-YO175 and EPS-OF101. The EPS samples showed strong antioxidant activities in-vitro. The scale-up of the production process of the EPS will find its potential application in food industries.

scholarly journals Production of exopolysaccharide by strains of Lactobacillus plantarum YO175 and OF101 isolated from traditional fermented cereal beverage

2018 ◽  
Author(s):  
Adekemi Titilayo Adesulu-Dahunsi ◽  
Kumaraswamy Jeyaram ◽  
Abiodun Isiaka Sanni
Keyword(s):  
Lactobacillus Plantarum ◽  
Antioxidant Activities ◽  
Scale Up ◽  
Food Industries ◽  
Ft Ir ◽  
Experimental Values ◽  
Modified Media ◽  
Central Composite ◽  
Ft Ir Spectroscopy

Lactobacillus plantarum YO175 and OF101 isolates from Nigerian traditional fermented cereal gruel ‘ogi’, were investigated on the basis of their capability to produce an exopolysaccharide (EPS) on sucrose modified media. Functional groups analysis of the EPSs produced (EPS-YO175 and EPS-OF101) by Fourier-transform infrared (FT-IR) spectroscopy revealed the presence of –OH, C=O and C-H groups. The chemical composition of EPS-YO175 and EPS-OF101 showed the presence of 87.1% and 80.62% carbohydrates and 1.21% and 1.47% protein. For maximum EPS yield, three significant factors were optimized using central composite design and response surface methodology, the predicted maximum EPS produced was 1.38 g/L and 2.19 g/L, while the experimental values were 1.36g/L and 2.18g/L for EPS-YO175 and EPS-OF101. The EPS samples showed strong antioxidant activities in-vitro. The scale-up of the production process of the EPS will find its potential application in food industries.

scholarly journals Antioxidant activity and green synthesis of selenium nanoparticles using allium sativum extract

2017 ◽  
Vol 9 (4) ◽  
pp. 634 ◽  
Author(s):  
Jay Vyas ◽  
Shafkat Rana
Keyword(s):  
Green Synthesis ◽  
Cost Effective ◽  
Selenium Nanoparticles ◽  
Spherical Particles ◽  
Frap Assay ◽  
Environment Friendly ◽  
Synthesis Of Nanoparticles ◽  
Transmission Electron ◽  
Ft Ir ◽  
Uv Visible

<p><strong>Abstract</strong></p><p>The green synthesis of nanoparticles has provided cost effective, environment friendly procedure and raising safe strategies for synthesis of non-materials. In this study we investigated the clove of <em>Allium sataivum</em>, which is used for the synthesis of Selenium nanoparticles. The biosynthesized selenium nanoparticles were characterized by using UV-Visible (UV-VIS) spectrophotometer, Transmission electron microscopy (TEM), Fourier transform spectroscopy (FT-IR) and Energy dispersive X-Ray spectroscopy (EDAX). The selenium nanoparticles synthesized by garlic were observed as hollow and spherical particles in size ranging 7-45nm which is found more stable more than two months. The present study also reports the efficiency of ABTS, DPPH and FRAP assay to estimate the antioxidant potential of selenium nanoparticles which synthesized by garlic extract.</p><p> </p>

scholarly journals Production of exopolysaccharide by strains of Lactobacillus plantarum YO175 and OF101 isolated from traditional fermented cereal beverage

2018 ◽  
Author(s):  
Adekemi Titilayo Adesulu-Dahunsi ◽  
Kumaraswamy Jeyaram ◽  
Abiodun Isiaka Sanni
Keyword(s):  
Lactobacillus Plantarum ◽  
Antioxidant Activities ◽  
Scale Up ◽  
Food Industries ◽  
Ft Ir ◽  
Experimental Values ◽  
Modified Media ◽  
Central Composite ◽  
Ft Ir Spectroscopy

Lactobacillus plantarum YO175 and OF101 isolates from Nigerian traditional fermented cereal gruel ‘ogi’, were investigated on the basis of their capability to produce an exopolysaccharide (EPS) on sucrose modified media. Functional groups analysis of the EPSs produced (EPS-YO175 and EPS-OF101) by Fourier-transform infrared (FT-IR) spectroscopy revealed the presence of –OH, C=O and C-H groups. The chemical composition of EPS-YO175 and EPS-OF101 showed the presence of 87.1% and 80.62% carbohydrates and 1.21% and 1.47% protein. For maximum EPS yield, three significant factors were optimized using central composite design and response surface methodology, the predicted maximum EPS produced was 1.38 g/L and 2.19 g/L, while the experimental values were 1.36g/L and 2.18g/L for EPS-YO175 and EPS-OF101. The EPS samples showed strong antioxidant activities in-vitro. The scale-up of the production process of the EPS will find its potential application in food industries.

Recent Advances on the Green Synthesis and Antioxidant Activities of Pyrazoles

2015 ◽  
Vol 14 (13) ◽  
pp. 1078-1092 ◽  
Author(s):  
Daiane Dias ◽  
Bruna Pacheco ◽  
Wilson Cunico ◽  
Lucas Pizzuti ◽  
Claudio Pereira
Keyword(s):  
Green Synthesis ◽  
Antioxidant Activities ◽  
Recent Advances

scholarly journals 111 Artificial Light-Triggered Sleep Deprivation May Lead to Allodynia in Rodent Model

SLEEP ◽  
2021 ◽  
Vol 44 (Supplement_2) ◽  
pp. A45-A46
Author(s):  
Skyler Kanegi ◽  
Armen Akopian
Keyword(s):  
Sleep Deprivation ◽  
Light Exposure ◽  
Light Stimulus ◽  
Continuous Light ◽  
Artificial Light ◽  
Light Cycle ◽  
Tissue Samples ◽  
Mechanical Threshold ◽  
Dim Light ◽  
Von Frey Filaments

Abstract Introduction The combination of artificial light and lack of exposure to natural light can delay the circadian clock, dysregulate the circadian cycle, and decrease alertness upon waking. This effect has been especially significant during the COVID-19 pandemic, where overexposure to artificial light at improper hours has contributed to increased rates of clinical insomnia. Artificial light may also contribute to concomitant neurological conditions such as primary headache, but the mechanisms by which light triggers sleep deprivation-induced headache are not well-understood. Methods To measure pain sensitivity, we habituated 13 wild-type male mice to von Frey filaments applied to the periorbital area until there was no response to 0.6g stimulus. We then applied 5 lux of continuous dim light to mice during their usual 12-hour dark cycle. The 12-hour light cycle remained unchanged with 200 lux continuous light. Three groups of mice experienced the dim light stimulus for one, three, or five consecutive days. Ambulation and rest activity were measured using SOF-812 Activity Monitor machines. After the experiment concluded, we waited 24 hours and measured mechanical threshold using von Frey filaments at 1, 3, 5, 8, and every 3 days subsequently until mice no longer responded to 0.6g stimulus. Results Artificial light triggered changes in circadian behavior including increased number of rest periods during 12-hour dark (dim light) cycle and shortened sleep duration during 12-hour light cycle. Following the artificial light stimulus, there was a significant decrease in mechanical threshold (P&lt;0.05), representing allodynia. The one-day group displayed one day of significant allodynia. The three-day group displayed three days of significant allodynia. The five-day group displayed five days of significant allodynia. Conclusion Artificial light may trigger circadian dysregulation, and the duration of artificial light exposure seemed to be directly correlated to the duration of allodynia up to one week after the stimulus was removed. We will repeat these experiments and analyze CNS and PNS tissue samples to understand the underlying physiological and biochemical bases of how artificial light triggers sleep deprivation-induced headache. This knowledge could increase our understanding of the pathophysiology and comorbidity of sleep deprivation and headache. Support (if any) Funding was received from the National Institute of Health (NS104200).

scholarly journals Green Synthesis, Characterization, and Antibacterial Properties of Silver Nanoparticles Obtained by Using Diverse Varieties of Cannabis sativa Leaf Extracts

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4041
Author(s):  
Adriana Cecilia Csakvari ◽  
Cristian Moisa ◽  
Dana G. Radu ◽  
Leonard M. Olariu ◽  
Andreea I. Lupitu ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Antioxidant Capacity ◽  
Cannabis Sativa ◽  
Antibacterial Properties ◽  
Green Building ◽  
Human Pathogens ◽  
Leaf Extracts ◽  
Vis Spectroscopy ◽  
Ft Ir

Cannabis sativa L. (hemp) is a plant used in the textile industry and green building material industry, as well as for the phytoremediation of soil, medical treatments, and supplementary food products. The synergistic effect of terpenes, flavonoids, and cannabinoids in hemp extracts may mediate the biogenic synthesis of metal nanoparticles. In this study, the chemical composition of aqueous leaf extracts of three varieties of Romanian hemp (two monoecious, and one dioecious) have been determined by Fourier-Transformed Infrared spectroscopy (FT-IR), high-performance liquid chromatography, and mass spectrometry (UHPLC-DAD-MS). Then, their capability to mediate the green synthesis of silver nanoparticles (AgNPs) and their pottential antibacterial applications were evaluated. The average antioxidant capacity of the extracts had 18.4 ± 3.9% inhibition determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 78.2 ± 4.1% determined by 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS™) assays. The total polyphenolic content of the extracts was 1642 ± 32 mg gallic acid equivalent (GAE) L−1. After this, these extracts were reacted with an aqueous solution of AgNO3 resulting in AgNPs, which were characterized by UV−VIS spectroscopy, FT-IR, scanning electron microscopy (SEM-EDX), and dynamic light scattering (DLS). The results demonstrated obtaining spherical, stable AgNPs with a diameter of less than 69 nm and an absorbance peak at 435 nm. The mixture of extracts and AgNPs showed a superior antioxidant capacity of 2.3 ± 0.4% inhibition determined by the DPPH• assay, 88.5 ± 0.9% inhibition as determined by the ABTS•+ assay, and a good antibacterial activity against several human pathogens: Escherichia coli, Klebsiella pneumoniae, Pseudomonas fluorescens, and Staphylococcus aureus.

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