scholarly journals Stereological Study of Changes of GABA-Immunoreactive Neurons in Spinal Dorsal Horn of SNI Rats

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Hong Liu ◽  
Weidong Li ◽  
Binbing Xu ◽  
Jiduan Jiang ◽  
Yuanyuan Zhang ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Nerve Trunk ◽  
Gabaergic Neurons ◽  
Withdrawal Threshold ◽  
Spinal Dorsal ◽  
Sd Rats ◽  
Significant Difference ◽  
Group D

Objective. To observe the changes in the mechanical withdrawal threshold (MWT) and the proportion of GABA-immunoreactive neurons in spinal dorsal horn (SDH) of the spared nerve injury (SNI) rat model. Methods. Thirty-six healthy male SD rats were randomly divided into a sham-operated group (group D, n = 18 ) and an SNI group (group S, n = 18 ). The left sciatic nerve trunk and three branches were exposed, two of which, known as tibial and the peroneal nerve, were ligated and cut off. The sural nerve was preserved to build the SNI model in group S. The left sciatic nerve trunk and three branches were only exposed in group D. MWT tests were performed on the medial and lateral sides of the rats’ left hindpaw 1 day before surgery and at 7th, 14th, and 28th day after surgery. Results. In group S, compared with the baseline measured 1 day before surgery, MWT on the medial and lateral sides of the rats’ left hindpaw decreased significantly on the 7th, 14th, and 28th days after surgery ( P < 0.05 ), while in group D, there was no statistically significant difference ( P > 0.05 ). Compared with right SDH, there were not statistically significant reductions in the proportions of GABAergic neurons of left SDH on 7th and 28th day after SNI ( P > 0.05 ); however, the proportion of GABAergic neurons in left SDH significantly decreased, compared with that in right side on 14th day after SNI ( P < 0.05 ). On the same way, the proportions of GABAergic neurons on 7th, 14th, and 28th day after surgery were not statistically different ( P > 0.05 ) in group D. Conclusion. The SNI model could reduce the proportion of GABA-immunoreactive neurons in the rat’s spinal dorsal horn on the nerve-injured side, and this change was lasting, which might be related to the transformation of the GABA-immunoreactive neurons.

scholarly journals Preproenkephalin mRNA is Expressed in a Subpopulation of GABAergic Neurons in the Spinal Dorsal Horn of the GAD67-GFP Knock-In Mouse

2008 ◽  
Vol 291 (10) ◽  
pp. 1334-1341 ◽  
Author(s):  
Jing Huang ◽  
Yayun Wang ◽  
Wei Wang ◽  
Yanyan Wei ◽  
Yunqing Li ◽  
...  
Keyword(s):  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Gabaergic Neurons ◽  
Spinal Dorsal

scholarly journals Metabotropic glutamate receptor 5 regulates excitability and Kv4.2-containing K+ channels primarily in excitatory neurons of the spinal dorsal horn

2011 ◽  
Vol 105 (6) ◽  
pp. 3010-3021 ◽  
Author(s):  
Hui-Juan Hu ◽  
Robert W. Gereau
Keyword(s):  
Spinal Cord ◽  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Neuronal Excitability ◽  
Gabaergic Neurons ◽  
Erk Signaling ◽  
Metabotropic Glutamate ◽  
Dorsal Horn Neurons ◽  
Spinal Dorsal ◽  
Excitatory Neurons

Metabotropic glutamate (mGlu) receptors play important roles in the modulation of nociception. Previous studies demonstrated that mGlu5 modulates nociceptive plasticity via activation of ERK signaling. We have reported recently that the Kv4.2 K+ channel subunit underlies A-type currents in spinal cord dorsal horn neurons and that this channel is modulated by mGlu5-ERK signaling. In the present study, we tested the hypothesis that modulation of Kv4.2 by mGlu5 occurs in excitatory spinal dorsal horn neurons. With the use of a transgenic mouse strain expressing enhanced green fluorescent protein (GFP) under control of the promoter for the γ-amino butyric acid (GABA)-synthesizing enzyme, glutamic acid decarboxylase 67 (GAD67), we found that these GABAergic neurons express less Kv4.2-mediated A-type current than non-GAD67-GFP neurons. Furthermore, the mGlu1/5 agonist, (R,S)-3,5-dihydroxyphenylglycine, had no modulatory effects on A-type currents or neuronal excitability in this subgroup of GABAergic neurons but robustly modulated A-type currents and neuronal excitability in non-GFP-expressing neurons. Immunofluorescence studies revealed that Kv4.2 was highly colocalized with markers of excitatory neurons, such as vesicular glutamate transporter 1/2, PKCγ, and neurokinin 1, in cultured dorsal horn neurons. These results indicate that mGlu5-Kv4.2 signaling is associated with excitatory dorsal horn neurons and suggest that the pronociceptive effects of mGlu5 activation in the spinal cord likely involve enhanced excitability of excitatory neurons.

scholarly journals Early Changes in Homer1 Proteins in the Spinal Dorsal Horn Are Associated with Loose Ligation of the Rat Sciatic Nerve

2009 ◽  
Vol 109 (6) ◽  
pp. 2000-2007 ◽  
Author(s):  
Gordana Miletic ◽  
Ashley M. Driver ◽  
Takako Miyabe-Nishiwaki ◽  
Vjekoslav Miletic
Keyword(s):  
Sciatic Nerve ◽  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Spinal Dorsal ◽  
Rat Sciatic Nerve

scholarly journals Loose ligation of the rat sciatic nerve elicits early accumulation of Shank1 protein in the post-synaptic density of spinal dorsal horn neurons

Pain ◽  
2010 ◽  
Vol 149 (1) ◽  
pp. 152-159 ◽  
Author(s):  
Gordana Miletic ◽  
Catalina I. Dumitrascu ◽  
Christopher E. Honstad ◽  
Daniela Micic ◽  
Vjekoslav Miletic
Keyword(s):  
Sciatic Nerve ◽  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Synaptic Density ◽  
Dorsal Horn Neurons ◽  
Spinal Dorsal ◽  
Rat Sciatic Nerve ◽  
Spinal Dorsal Horn Neurons ◽  
Post Synaptic Density

Melatonin Reduce Morphine-Induced Hyperalgesia and Tolerance of Rats via melatonin-MT1-PKCγ Pathway.

2020 ◽  
Author(s):  
Yuchao Fan ◽  
Xiao Liang ◽  
Bixin Zheng ◽  
Li Song
Keyword(s):  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Morphine Tolerance ◽  
Melatonin Level ◽  
Chronic Morphine ◽  
Lower Serum ◽  
Regulation Of Expression ◽  
Spinal Dorsal ◽  
Sd Rats ◽  
Morphine Administration

Abstract Background: Morphine is commonly used for treating acute and chronic pain. However, its using is complicated with tolerance and hyperalgesia. Endogenous melatoninergic system is involved in development of tolerance and hyperalgesia induced by chronic morphine administration, but the precise mechanism remains unknown. Methods: 18 male SD rats were randomly divided into Saline(Sal), Morphine(Mor) and Morphine+Chelerythrine (Mor+Che) group (n = 6, respectively). Each group were received twice-daily intrathecal injections of saline (10μl), morphine (15μg/10μl) or morphine (15μg/5μl)+ chelerythrine (5μg/5μl) for consecutive 9 days, respectively. MWT and TWL of all animals were recorded on day 1, 3, 5, 7, 9. The morphine tolerance was depicted as MPAE on day 1, 3, 5, 7, 9 and on day 10 which can be used to calculate for ED50 of each group. Then we determined the serum level of melatonin by ELISA and expression of MOR , MT1, MT2 and PKCγ by RT-qPCR and WB in spinal dorsal horn of three group rats.Results: Comparing with Sal group, the Mor group exhibited a significant lower serum melatonin level and up-regulation of expression of the MT1, MT2 and PKCγ in the spinal dorsal horn. Co-administration of chelerythrine with morphine not only attenuates morphine-induced hyperalgesia and tolerance, but also increases the down-regulation of serum melatonin level) and reduces the up-regulation of expression of MT1 and PKCγ in spinal dorsal horn compared with Mor group. Conclusion：Melatonin can reduce morphine-induced hyperalgesia and tolerance of rats via melatonin-MT1-PKCγ pathway.

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