scholarly journals Preproenkephalin mRNA is Expressed in a Subpopulation of GABAergic Neurons in the Spinal Dorsal Horn of the GAD67-GFP Knock-In Mouse

2008 ◽  
Vol 291 (10) ◽  
pp. 1334-1341 ◽  
Author(s):  
Jing Huang ◽  
Yayun Wang ◽  
Wei Wang ◽  
Yanyan Wei ◽  
Yunqing Li ◽  
...  
Keyword(s):  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Gabaergic Neurons ◽  
Spinal Dorsal

scholarly journals Metabotropic glutamate receptor 5 regulates excitability and Kv4.2-containing K+ channels primarily in excitatory neurons of the spinal dorsal horn

2011 ◽  
Vol 105 (6) ◽  
pp. 3010-3021 ◽  
Author(s):  
Hui-Juan Hu ◽  
Robert W. Gereau
Keyword(s):  
Spinal Cord ◽  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Neuronal Excitability ◽  
Gabaergic Neurons ◽  
Erk Signaling ◽  
Metabotropic Glutamate ◽  
Dorsal Horn Neurons ◽  
Spinal Dorsal ◽  
Excitatory Neurons

Metabotropic glutamate (mGlu) receptors play important roles in the modulation of nociception. Previous studies demonstrated that mGlu5 modulates nociceptive plasticity via activation of ERK signaling. We have reported recently that the Kv4.2 K+ channel subunit underlies A-type currents in spinal cord dorsal horn neurons and that this channel is modulated by mGlu5-ERK signaling. In the present study, we tested the hypothesis that modulation of Kv4.2 by mGlu5 occurs in excitatory spinal dorsal horn neurons. With the use of a transgenic mouse strain expressing enhanced green fluorescent protein (GFP) under control of the promoter for the γ-amino butyric acid (GABA)-synthesizing enzyme, glutamic acid decarboxylase 67 (GAD67), we found that these GABAergic neurons express less Kv4.2-mediated A-type current than non-GAD67-GFP neurons. Furthermore, the mGlu1/5 agonist, (R,S)-3,5-dihydroxyphenylglycine, had no modulatory effects on A-type currents or neuronal excitability in this subgroup of GABAergic neurons but robustly modulated A-type currents and neuronal excitability in non-GFP-expressing neurons. Immunofluorescence studies revealed that Kv4.2 was highly colocalized with markers of excitatory neurons, such as vesicular glutamate transporter 1/2, PKCγ, and neurokinin 1, in cultured dorsal horn neurons. These results indicate that mGlu5-Kv4.2 signaling is associated with excitatory dorsal horn neurons and suggest that the pronociceptive effects of mGlu5 activation in the spinal cord likely involve enhanced excitability of excitatory neurons.

Quantitative study of NPY-expressing GABAergic neurons and axons in rat spinal dorsal horn

2011 ◽  
Vol 519 (6) ◽  
pp. spc1-spc1
Author(s):  
Erika Polgár ◽  
Thomas C. P. Sardella ◽  
Masahiko Watanabe ◽  
Andrew J. Todd
Keyword(s):  
Dorsal Horn ◽  
Quantitative Study ◽  
Spinal Dorsal Horn ◽  
Gabaergic Neurons ◽  
Spinal Dorsal

Connections between EM2- and SP-containing terminals and GABAergic neurons in the mouse spinal dorsal horn

2014 ◽  
Vol 35 (9) ◽  
pp. 1421-1427 ◽  
Author(s):  
Dao-Shu Luo ◽  
Jing Huang ◽  
Yu-Lin Dong ◽  
Zhen-Yu Wu ◽  
Yan-Yan Wei ◽  
...  
Keyword(s):  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Gabaergic Neurons ◽  
Spinal Dorsal

Facilitatory Actions of Serotonin Type 3 Receptors on GABAergic Inhibitory Synaptic Transmission in the Spinal Superficial Dorsal Horn

2009 ◽  
Vol 102 (3) ◽  
pp. 1459-1471 ◽  
Author(s):  
Teruyuki Fukushima ◽  
Toshiki Ohtsubo ◽  
Masayuki Tsuda ◽  
Yuchio Yanagawa ◽  
Yuuichi Hori
Keyword(s):  
Synaptic Transmission ◽  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Gabaergic Neurons ◽  
Pcr Analysis ◽  
Superficial Dorsal Horn ◽  
Inhibitory Synaptic Transmission ◽  
Spinal Dorsal ◽  
Presynaptic Mechanisms ◽  
Type 3

Analgesic effects of serotonin (5-hydroxytryptamine [5-HT]) type 3 (5-HT3) receptors may involve the release of γ-aminobutyric acid (GABA) in the spinal dorsal horn. However, the precise synaptic mechanisms for 5-HT3 receptor-mediated spinal analgesia are not clear. In this study, we investigated whether GABAergic neurons in the superficial dorsal horn (SDH) express functional 5-HT3 receptors and how these 5-HT3 receptors affect GABAergic inhibitory synaptic transmission in the SDH, by using slice preparations from adult glutamate decarboxylase 67–green fluorescent protein (GAD67-GFP) knock-in mice. Tight-seal whole cell recordings from GFP-positive and -negative neurons showed that 5-HT3 receptor-specific agonist 2-methyl-serotonin (2-Me-5-HT) induced inward currents in a substantial population of both GFP-positive and -negative neurons. Additionally, we confirmed expression of 5-HT3 receptors in both types of neurons by single-cell reverse transcription–polymerase chain reaction (RT-PCR) analysis. Further, GABAA receptor-mediated inhibitory postsynaptic currents (IPSCs)—both those evoked by electrical stimulation and those occurring spontaneously in tetrodotoxin (i.e., miniature IPSCs [mIPSCs])—were recorded from GFP-negative neurons. 2-Me-5-HT increased the amplitude of the evoked IPSCs and the frequency of mIPSCs. The amplitude of mIPSCs was not affected by 2-Me-5-HT, suggesting that 5-HT augments GABAergic synaptic transmission via presynaptic mechanisms. The present observations indicate that 5-HT3 receptors are expressed on both somadendritic regions and presynaptic terminals of GABAergic neurons and regulate GABAA receptor-mediated inhibitory synaptic transmission in the SDH. Taken together, these results provide clues for the underlying mechanisms of the antinociceptive actions of 5-HT3 receptors in the spinal dorsal horn.

scholarly journals Quantitative study of NPY‐expressing GABAergic neurons and axons in rat spinal dorsal horn

2011 ◽  
Vol 519 (6) ◽  
pp. 1007-1023 ◽  
Author(s):  
Erika Polgár ◽  
Thomas C.P. Sardella ◽  
Masahiko Watanabe ◽  
Andrew J. Todd
Keyword(s):  
Dorsal Horn ◽  
Quantitative Study ◽  
Spinal Dorsal Horn ◽  
Gabaergic Neurons ◽  
Spinal Dorsal
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