scholarly journals Nocardia thailandica Brain Abscess in an Immunocompromised Patient

2021 ◽  
Vol 2021 ◽  
pp. 1-4
Author(s):  
Muhammad Effendi ◽  
Samad Tirmizi ◽  
Dayna McManus ◽  
Anita J. Huttner ◽  
David R. Peaper ◽  
...  
Keyword(s):  
Brain Abscess ◽  
Successful Treatment ◽  
Medical Literature ◽  
Immunocompromised Patient ◽  
Treatment Success ◽  
Cns Infection ◽  
16S Rdna Sequencing ◽  
Rdna Sequencing ◽  
Cns Penetration ◽  
Initiation Of Therapy

Objectives. Successful treatment for Nocardia thailandica is not well elucidated in the literature. To the best of our knowledge, N. thailandica has not yet been described in the medical literature to cause central nervous system (CNS) infection from brain abscess. We report the case of an immunocompromised patient who underwent successful treatment to treat his brain abscess caused by N. thailandica. Methods. After failing medical therapy, the patient underwent a craniotomy, and tissue was sent for culture. Upon identification by 16S rDNA sequencing, the organism causing infection was identified to be N. thailandica. Results. Based on susceptibilities, the patient was treated with IV ceftriaxone 2 grams daily for five months. The patient demonstrated clinical and radiological improvement which persisted to 7 months after initiation of therapy. Conclusions. To the best of our knowledge, this is the first documented case of a brain abscess due to N. thailandica which was successfully treated. Due to the location of the infection, ceftriaxone was chosen because of optimal CNS penetration. Ceftriaxone monotherapy demonstrated clinical and radiographic treatment success resulting in the successful treatment of this infection.

Successful treatment of brain abscess caused byNocardia in an immunocompromised patient after failure of co-trimoxazole

Infection ◽  
1992 ◽  
Vol 20 (6) ◽  
pp. 365-366 ◽  
Author(s):  
D. Overkamp ◽  
B. Waldmann ◽  
T. Lins ◽  
Th. Lingenfelser ◽  
M. Eggstein ◽  
...  
Keyword(s):  
Brain Abscess ◽  
Successful Treatment ◽  
Immunocompromised Patient

Metabolite fingerprinting of novel Streptomyces UK-238 from the Himalayan forest

2020 ◽  
Vol 16 ◽  
Author(s):  
Nidhi Srivastava ◽  
Indira P. Sarethy
Keyword(s):  
Ethyl Acetate ◽  
16S Rdna ◽  
Retention Index ◽  
Ethyl Acetate Extract ◽  
Similarity Index ◽  
Antimicrobial Drug ◽  
16S Rdna Sequencing ◽  
Metabolite Fingerprinting ◽  
Streptomyces Sp ◽  
Rdna Sequencing

Aims: Characterization of antimicrobial metabolites of novel Streptomyces sp. UK-238. Background: Novel antimicrobial drug discovery is urgently needed due to emerging multi antimicrobial drug resistance among pathogens. Since many years, natural products have provided the basic skeletons for many therapeutic compounds including antibiotics. Bioprospection of un/under explored habitats and focussing on selective isolation of actinobacteria as major reservoir of bio and chemodiversity has yielded good results. Objective: The main objectives of the study were the identification of UK-238 by 16S rDNA sequencing and antimicrobial metabolite fingerprinting of culture extracts. Method: In the present study, a promising isolate, UK-238, has been screened for antimicrobial activity and metabolite fingerprinting from the Himalayan Thano Reserve forest. It was identified by 16S rDNA sequencing. Ethyl acetate extract was partially purified by column chromatography. The pooled active fractions were fingerprinted by GC-MS and compounds were tentatively identified by collated data analysis based on Similarity Index, observed Retention Index from Databases and calculated Retention Index. Results: UK-238 was identified as Streptomyces sp. with 98.4% similarity to S. niveiscabiei. It exhibited broad-spectrum antibacterial and antifungal activity. GC-MS analysis of active fractions of ethyl acetate extract showed the presence of eighteen novel antimicrobial compounds belonging to four major categories- alcohols, alkaloid, esters and peptide. Conclusion: The study confirms that bioprospection of underexplored habitats can elaborate novel bio and chemodiversity.

PSVII-9 Influence of lactose and milk oligosaccharides in whey permeate on jejunal mucosa-associated microbiota in nursery pigs during 7 to 11 kg BW

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 407-407
Author(s):  
Ki Beom Jang ◽  
Sung Woo Kim
Keyword(s):  
Alpha Diversity ◽  
Jejunal Mucosa ◽  
16S Rdna Sequencing ◽  
Sequencing Data ◽  
Milk Oligosaccharides ◽  
Whey Permeate ◽  
Nursery Pigs ◽  
Rdna Sequencing ◽  
Acid Producing Bacteria ◽  
Diversity Estimates

Abstract This study aimed to evaluate supplemental effects of milk carbohydrates in whey permeate on jejunal mucosa-associated microbiota in nursery pigs during 7 to 11 kg BW. A total of 720 pigs at 7.5 kg BW were allotted to 6 treatments (6 pens/treatment and 20 pigs/pen). Treatments were 6 levels of whey permeate supplementation (0, 3.75, 7.50, 11.25, 15.00, and 18.75%) and fed to pigs for 11 d. On d 11, 36 pigs representing median BW of each pen were euthanized to collect the jejunal mucosa to evaluate microbiota in the jejunum by 16S rDNA sequencing. Data were analyzed using contrasts in MIXED procedure of SAS. Whey permeate contained 76.3% lactose and 0.4% milk oligosaccharides. Increasing whey permeate supplementation from 0 to 18.75% did not affect the alpha-diversity estimates of microbiota. Whey permeate supplementation tended to decrease (P = 0.073, 1.59 to 1.22) Firmicutes:Bacteroidetes compared with no addition of whey permeate. Increasing whey permeate supplementation tended to linearly increase Bifidobacteriaceae (P = 0.089, 0.73 to 1.11), decrease Enterobacteriaceae (P = 0.091, 1.04 to 0.52), decrease Stretococcaceae (P = 0.094, 1.50 to 0.71), and caused quadratic changes (P < 0.05) on Lactobacillaceae (maximum: 9.14% at 12.91% whey permeate). Increasing whey permeate supplementation caused a quadratic change (P < 0.05) on Lactobacillus_Salivarius (maximum: 0.92% at 7.35% whey permeate) and tended to cause quadratic changes on Lactobacillus_Rogosae (P = 0.083; maximum: 0.53% at 8.45% whey permeate) and Lactobacillus_Mucosae (P = 0.092; maximum: 0.70% at 6.98% whey permeate). In conclusion, supplementation of whey permeate as sources of lactose and milk oligosaccharides at a range from 7 to 13% seems to be beneficial to nursery pigs by increasing the abundance of lactic acid-producing bacteria in the jejunal mucosa.

scholarly journals 2-Way k-Means as a Model for Microbiome Samples

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Weston J. Jackson ◽  
Ipsita Agarwal ◽  
Itsik Pe’er
Keyword(s):  
Unsupervised Learning ◽  
16S Rdna ◽  
Mixture Model ◽  
Vaginal Microbiome ◽  
16S Rdna Sequencing ◽  
Sequencing Data ◽  
Cluster Assignment ◽  
Rdna Sequencing

Motivation. Microbiome sequencing allows defining clusters of samples with shared composition. However, this paradigm poorly accounts for samples whose composition is a mixture of cluster-characterizing ones and which therefore lie in between them in the cluster space. This paper addresses unsupervised learning of 2-way clusters. It defines a mixture model that allows 2-way cluster assignment and describes a variant of generalized k-means for learning such a model. We demonstrate applicability to microbial 16S rDNA sequencing data from the Human Vaginal Microbiome Project.

Analysis of the bacterial floral structure and diversity of Xuanwei ham by 16S rDNA sequencing

2020 ◽  
Vol 40 (4) ◽  
Author(s):  
Luying Shan ◽  
Yinjiao Li ◽  
Shi Zheng ◽  
Yuanmiao Wei ◽  
Ying Shang
Keyword(s):  
16S Rdna ◽  
16S Rdna Sequencing ◽  
Floral Structure ◽  
Rdna Sequencing

scholarly journals ISOLATION, PURIFICATION, AND CHARACTERIZATION OF LIPASE FROM BACILLUS SP. FROM KITCHEN GREASE

2018 ◽  
Vol 11 (6) ◽  
pp. 224
Author(s):  
Jaiganesh R ◽  
Jaganathan Mk
Keyword(s):  
16S Rdna ◽  
Solvent Tolerance ◽  
16S Rdna Sequencing ◽  
Bacillus Sp ◽  
Purification And Characterization ◽  
Lipase Enzyme ◽  
Sequencing Method ◽  
Rdna Sequencing ◽  
Solvent Tolerant

Objective: The objective of this work was to isolation, purification and characterization of solvent tolerant lipase from Bacillus sp. The objective of this work was to isolation, purification and characterization of solvent tolerant lipase from Bacillus sp. from kitchen grease for a variety of applications including organic synthetic reactions and preparation of enantiomerically pure pharmaceuticals.Methods: Lipase producing isolates were screened from kitchen grease on a selective medium rhodamine B olive oil agar, and tributyrin agar was used to screen the lipase and esterase producing an organism, respectively. The isolate identified using 16S rDNA sequencing method and enzyme activity was quantitatively assayed. Lipase production was characterized in different conditions.Results: The isolate showed highest lipase activity was which later was identified as Bacillus sp. using 16S rDNA sequencing method. The lipase was purified using ammonium sulfate precipitation. The isolate showed excellent tolerance to methanol, ethanol, acetonitrile, and moderate tolerance to butanol. The increased biomass concentration, maximum production, and activity were achieved at 37°C in 24 h incubation, then gradual reduction in production was observed. The maximum activity of lipase enzyme was obtained at pH between 6 and 9.Conclusion: The isolate produce solvent tolerance lipase enzyme and it can be a promising candidate of solvent tolerance lipase enzyme for variety of industrial applications.

Gut microbiota community adaption during young children fecal microbiota transplantation by 16s rDNA sequencing

2016 ◽  
Vol 206 ◽  
pp. 66-72 ◽  
Author(s):  
Jian-Lei Gu ◽  
Yi-Zhong Wang ◽  
Shi-Yi Liu ◽  
Guang-Jun Yu ◽  
Ting Zhang ◽  
...  
Keyword(s):  
Young Children ◽  
Gut Microbiota ◽  
16S Rdna ◽  
Fecal Microbiota Transplantation ◽  
Fecal Microbiota ◽  
16S Rdna Sequencing ◽  
Rdna Sequencing

scholarly journals Determination of uterine bacterial community in postpartum dairy cows with metritis based on 16S rDNA sequencing

2020 ◽  
Vol 10 ◽  
pp. 100102
Author(s):  
Hao Chen ◽  
Kaiqiang Fu ◽  
Binbin Pang ◽  
Jifang Wang ◽  
Huatao Li ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Dairy Cows ◽  
16S Rdna ◽  
16S Rdna Sequencing ◽  
Rdna Sequencing

scholarly journals Detection of Arcobacter spp. in Mytilus galloprovincialis samples collected from Apulia region

2015 ◽  
Vol 4 (1) ◽  
Author(s):  
Elisabetta Bonerba ◽  
Anna Mottola ◽  
Antonio Parisi ◽  
Angela Di Pinto ◽  
Andrea Serraino ◽  
...  
Keyword(s):  
Mytilus Galloprovincialis ◽  
Disease Outbreak ◽  
16S Rdna Sequencing ◽  
Emerging Pathogen ◽  
Chain Reaction ◽  
Selective Enrichment ◽  
Apulia Region ◽  
Rdna Sequencing ◽  
Arcobacter Butzleri ◽  
Polymerase Chain

The aim of the study was to evaluate the occurrence of <em>Arcobacter</em> spp. in 20 samples of <em>Mytilus galloprovincialis</em> purchased at fish markets in Apulia region. The detection of <em>Arcobacter</em> spp. was performed, after selective enrichment, on modified charcoal cefoperazone deoxycholate (mCCD) agar supplemented with Cefoperazone, Amphotericin B and Teicoplanin (CAT). In 6 out of the 20 tested samples the presence of <em>Arcobacter</em> spp. was found and confirmed by genus-based polymerase chain reaction. All the isolates were identified as belonging to the species<em> Arcobacter butzleri</em> using 16S rDNA sequencing and BLAST online. The results represent the first report in Italy of <em>A. butzleri</em> detection in marketed <em>Mytilus galloprovincialis</em>. The survey underlines the epidemiological importance of <em>A. butzleri</em> as an emerging pathogen, and highlights that mussels should be considered as a potential cause of foodborne disease outbreak.

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