scholarly journals Nucleophilic Displacement Reaction on Tosyl Cellulose by L-Methionine to the Synthesis of Novel Water-Soluble Cellulose Derivative and Its Antibacterial Activity

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Lahcen El Hamdaoui ◽  
Ahmed Talbaoui ◽  
Mohammed El Moussaouiti
Keyword(s):  
Antibacterial Activity ◽  
Water Soluble ◽  
Cellulose Derivative ◽  
Proton Nuclear Magnetic Resonance ◽  
Diffusion Method ◽  
Esterification Reaction ◽  
Tosyl Chloride ◽  
Nucleophilic Displacement ◽  
Tosyl Group ◽  
Microbial Strains

A novel ampholytic cellulose derivative, cellulose-L-methionine, has been synthesized by means of an esterification reaction of microcrystalline cellulose with tosyl chloride (p-TsCl) in DMAc/LiCl (8%) at 8°C that was followed by nucleophilic displacement (SN) of the tosyl group by the L-methionine amino acid. The resulting structure of cellulose-L-methionine has been characterized by elemental analysis (CHNSO), Fourier-transform infrared spectroscopy (FTIR), proton nuclear magnetic resonance (1H-NMR), and scanning electron microscopy (SEM). The antibacterial activity of the synthesized product was screened against Gram-positive and Gram-negative microbial strains such as Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, by the agar well diffusion method, and compared with commercial antibiotics such as ampicillin and chloramphenicol. It was found that antibacterial experiment revealed excellent antibacterial activity of the cellulose-methionine with respect to a minimal inhibitory concentration (MIC) reference.

scholarly journals STUDY OF ANTIBACTERIAL ACTIVITY OF THE PREPARATIONS WITH DIOXIDINE AND LEVOFLOXACIN ON MAIN PURULENT-INFLAMMATORY PROCESSES PATHOGENS

2021 ◽  
Vol 74 (9) ◽  
pp. 2109-2111
Author(s):  
Evheniia A. Shtaniuk ◽  
Oleksandra O. Vovk ◽  
Larisa V. Krasnikova ◽  
Yuliia I. Polyvianna ◽  
Tetiana I. Kovalenko
Keyword(s):  
Antibacterial Activity ◽  
Water Soluble ◽  
Diffusion Method ◽  
E Coli ◽  
Mueller Hinton ◽  
Microbiological Methods ◽  
Pure Cultures ◽  
Inflammatory Processes ◽  
Definition Of

The aim: Study of antibacterial activity of the preparations, containing antiseptic dioxidine and antibiotic levofloxacin in vitro on standard strains of main optional-anaerobic pathogens of purulent-inflammatory processes of surgical wounds S. aureus, E. coli, P. aeruginosa and definition of more effective ones on them. Materials and methods: Solutions of dioxidine 1.2 %, dioxidine 1.2% with decamethaxin, Dioxisole, water soluble ointment with dioxidine 1.2% and levofloxacin 0.1% with decamethaxin were used in experiment. Antibacterial activity was studied on standard strains of S. aureus АТСС 25923, E. coli АТСС 25922, P. aeruginosa АТСС 27853. Distinguishing and identification of pure cultures of bacteria was done according to generally accepted microbiological methods. Determination of purulent-inflammatory processes pathogens sensitivity was done by disco-diffuse method on Mueller-Hinton medium. Antibacterial activity of solutions and ointments was studied with the help of agar diffusion method (“well” method) according to methodic recommendations. Each investigation was repeated 6 times. Method of variation statistics was used for the research results analysis. Results: All antibacterial preparations under study are effective and highly effective on S. aureus АТСС 25923, E. coli АТСС 25922, P. aeruginosa АТСС 27853. Solution with 1.2 % dioxidine with decamethaxin and ointment with 0.1 % levofloxacin and decamethaxin have larger growth retardation zones towards S. aureus and P. aeruginosa. E. coli strains are more sensitive to the solution of Dioxisole and ointment with 1.2 % dioxidine. Conclusions: All strains are sensitive, most of them are highly sensitive, up to 5 antibacterial preparations under study in vitro.

Synthesis of antibacterial silver–chitosan-modified bionanocomposites by RAFT polymerization and chemical reduction methods

2016 ◽  
Vol 49 (2) ◽  
pp. 173-193 ◽  
Author(s):  
Mojtaba Abbasian ◽  
Farideh Mahmoodzadeh
Keyword(s):  
Antibacterial Activity ◽  
Hybrid Materials ◽  
Chemical Reduction ◽  
Proton Nuclear Magnetic Resonance ◽  
Diffusion Method ◽  
Infrared Spectrometry ◽  
Resonance Spectroscopy ◽  
Ag Nps ◽  
Stabilizing Agent ◽  
X Ray

Chitosan (Cts) was first modified with 4-cyano,4-[(phenylcarbothioyl)sulfanyl]pentanoic acid to serve as reversible addition–fragmentation chain transfer (RAFT) agent, and then the controlled grafting polymerizations of acrylic acid (PAA) were performed. The resultant copolymers were used as a stabilizing agent for preparation of colloidal silver nanoparticles (Ag NPs) in the range of 2–10 nm. Afterwards, montmorillonites (MMTs) were added to the solution of colloidal Ag NPs for improving thermal stability. Proton nuclear magnetic resonance spectroscopy, Fourier transform infrared spectrometry, energy dispersive X-ray spectroscopy, X-ray diffraction analysis, and scanning electron microscopy images demonstrate the successful synthesis of the graft copolymer. Their thermal behavior was examined by differential scanning calorimetry and thermogravimetric analyses.The antibacterial activity of Ag/Cts-g-PAA and Ag/Cts-g-PAA/MMT was investigated against Staphylococcus aureus, Escherichia coli, and Candida albicans by the disc diffusion method using Mueller Hinton agar. Antimicrobial tests show that Ag/Cts-g-PAA has much higher antimicrobial activity than Ag/Cts-g-PAA/MMT. This method would enable a wide variety of molecular designs to afford novel types of tailored hybrid materials composed of natural polysaccharides and synthetic polymers. The new hybrid materials were used as a stabilizing agent for preparation of Ag bionanocomposites with good antibacterial activity.

scholarly journals ANTI BACTERIAL AND BIOFILM INHIBITORY ACTIVITIES OF AEGLE MARMELOS METHANOL LEAF EXTRACT

2021 ◽  
Vol 9 (09) ◽  
pp. 165-173
Author(s):  
Gujjari Sreehitha Pratap ◽  
Keyword(s):  
Antibacterial Activity ◽  
Biologically Active ◽  
Diffusion Method ◽  
Antibiofilm Activity ◽  
Phytochemical Analysis ◽  
Aegle Marmelos ◽  
Leaf Extracts ◽  
Biofilm Inhibition ◽  
Microbial Strains ◽  
Elisa Plate

The Aegle marmelos commonly known as BAEL belongs to family Rutaceae plays a role in traditional culture and medication from ancient periods. This plant lacks sufficient evidences regarding the values and components it has. Therefore, we framed out our studies to evaluate the phytochemical analysis, antibacterial activity, antibiofilm activity. These studies are evaluated using different solvents like methanol, acetone, chloroform, toluene leaf extracts of Aegle marmelos. We evaluated the potency of different solvents leaf extracts using Agar well diffusion method. Antibacterial activity was also evaluated using ELISA plate assay. The potency of different solvents extracts to inhibit biofilm of selected microbial strains. In accordance to results, the leaf extracts revealed the presence of several biologically active phytochemicals with highest quantities of carbohydrates, phenols, alkaloids, flavonoids, tannins, saponins, steroids, aminoacids etc. The antibacterial activity was found significant against microbial strains of both gram positive and gram negative bacteria. These strains showed susceptibility nature towards the different solvents extracts with zone of inhibitions (mm). On the other hand, the inhibition of biofilm was also significant at all tested concentrations. The biofilm inhibition of microbial strains was found significant at 1 XMIC, 2 XMIC, 3 XMIC. Based on our studies here we conclude that the different solvents leaf extracts possessed inhibitory activity against selected human pathogenic organisms.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN MELINJO (Gnetum gnemon L.) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus dan Streptococcus mutans TAHUN 2020

2020 ◽  
Vol 3 (1) ◽  
pp. 7-12
Author(s):  
Firdaus Fahdi ◽  
Herviani Sari
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Ash Content ◽  
Water Soluble ◽  
Diffusion Method ◽  
Soluble Extract ◽  
Gnetum Gnemon

The Gnetum gnemon can be used as a medicine that has the function of anti-bacterial. The chemical content inside which is anti-bacterial is flavonoids and tannins. This study aims to find out the antibacterial from the ethanol extract of melinjo leaves can inhibit the growth of Staphylococcus aureus and Streptococcus mutans with a concentration of 300 mg/ml, 400 mg/ml, and 500 mg/ml. The test of anti-bacterial activity for the growth Staphylococcus aureus and Streptococcus mutans is done by using the disc diffusion method. The results showed that melinjo leaf contains an alkaloid, flavonoid, tannin, steroid and saponin compounds. The results of the simplicia powder characteristics obtained a total ash content of 7.495%, ash content insoluble in acid 0.785%, ethanol-soluble extract 4.324%, water-soluble extract 17.690%. The results of the antibacterial activity test of the ethanol extract of melinjo leaves obtained can inhibit the growth of Staphylococcus aureus and Streptococcus mutans namely at a concentration of 300 mg/ml, 400 mg/ml, and 500 mg/ml into the medium inhibition zone category. Ethanol extract of melinjo leaves has antibacterial activity against the growth of Staphylococcus aureus and Streptococcus mutans with an average diameter of inhibition zone of 9.7 mm (medium category) against Staphylococcus aureus and 6.7 mm (medium category) against bacteria Streptococcus mutans.

Antimicrobial activity of Saponins rich fraction of Cassia auriculata Linn against various microbial strains

2013 ◽  
Vol 2 (4) ◽  
pp. 85-87
Author(s):  
Supriya Deshpande ◽  
Shailesh Kewatkar ◽  
Vivek Paithankar
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Infectious Diseases ◽  
Antimicrobial Effect ◽  
Pharmaceutical Journal ◽  
Diffusion Method ◽  
E Coli ◽  
Cassia Auriculata ◽  
Aeromonas Hydrophilia ◽  
Microbial Strains

In the present investigation, the saponins rich fraction of roots of Cassia auriculata L. was evaluated for antimicrobial activity against P. vesicularis, Streptococcus faecalis, Aeromonas hydrophilia, Salmonella typhae, Staphylococcus cohni, Serratia ficaria and E. coli at concentration of 12.5 mg/ml, 25 mg/ml, 37.5 mg/ml and 50 mg/ml. Antimicrobial activity of Cassia auriculata L. was carried out by well diffusion method. At maximum conc. i.e. 50mg/ml, antimicrobial effect of Saponin rich extract can be arranged in sequence of - P. vesicularis > Serratia ficaria > Streptococcus cohni > Aeromonas hydrophilic> Salmonella typhae > Sterptococcus faecalis > E. coli. The results indicate the saponins rich fraction of roots of Cassia auriculata L. might be exploited as natural drug for the treatment of several infectious diseases caused by these organisms. Cassia auriculata L. was observed to have antibacterial activity and can be used for medicinal purposes.DOI: http://dx.doi.org/10.3329/icpj.v2i4.14056 International Current Pharmaceutical Journal, March 2013, 2(4): 85-87 

scholarly journals Green Fabrication of Silver Nanoparticles by Gum Tragacanth (Astragalus gummifer): A Dual Functional Reductant and Stabilizer

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Aruna Jyothi Kora ◽  
Jayaraman Arunachalam
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Water Soluble ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
X Ray Diffraction ◽  
Gum Tragacanth ◽  
Synthesis Of Nanoparticles ◽  
Dual Functional ◽  
Transmission Electron

A simple and ecofriendly procedure have been devised for the green synthesis of silver nanoparticles using the aqueous extract of gum tragacanth (Astragalus gummifer), a renewable, nontoxic natural phyto-exudate. The water soluble components in the gum act as reductants and stabilizers. The generated nanoparticles were analyzed using UV-visible spectroscopy, transmission electron microscopy, X-ray diffraction, Fourier transform-infrared spectroscopy, and Raman spectroscopy. The role of gum concentration and reaction time on the synthesis of nanoparticles was studied. By regulating the reaction conditions, spherical nanoparticles of13.1±1.0 nm size were produced. Also, the possible functional groups involved in reduction and capping of nanoparticles has been elucidated. The antibacterial activity of the fabricated nanoparticles was tested on model Gram-negative and Gram-positive bacterial strains with well-diffusion method. These nanoparticles exhibited considerable antibacterial activity on both the Gram classes of bacteria, implying their potential biomedical applications.

scholarly journals Antibacterial Poly-Herbal Semisolid Formulations Containing Leaves Extracts of Tectona grandis, Mangifera indica and Anacardium occidentale

2016 ◽  
Vol 8 (05) ◽  
Author(s):  
K. Krishnananda Kamath ◽  
A. Shabaraya
Keyword(s):  
Antibacterial Activity ◽  
Mangifera Indica ◽  
Tectona Grandis ◽  
Dosage Forms ◽  
Water Soluble ◽  
Diffusion Method ◽  
E Coli ◽  
Zones Of Inhibition ◽  
Herbal Formulations ◽  
Agar Well Diffusion Method

Herbal medicine have become an item of global importance both has medicinal and economical value. Plants used for medicinal purposes long before recorded history. Many plant species have been proved to have antibacterial activity. Thus, the main objective of the study was to formulate and evaluate a poly-herbal semisolid dosage forms using ethanolic extracts of frontal leaves of T. grandis, M. indica and A. occidentale. Formulations were evaluated for its physicochemical properties like color, consistency, pH, spreadability, extrudability and the results were found satisfactory. Antibacterial activity of formulations was studied against S. aureus, E. coli and P. aeruginosa by agar well diffusion method. (E. coli, P. aeruginosa) bacteria. The order of activity was as follows: Water Soluble bases > Gel Bases > Hydrocarbon Bases. The zones of inhibition of poly herbal formulations were in between 23-28 mm which can be comparable with standard formulation 24-29 mm. Activity of the formulations may be due to the presence of alkaloids, flavanoids, phenols and tannins in the extracts. The formulations were found to be very efficacious in all the parameters.

Preliminary in Vitro Investigations on The Inhibitory Activity of The Original Dietary Supplement Oxidal® on Pathogenic Bacterial Strains

2020 ◽  
Vol 11 ◽  
pp. 37-43
Author(s):  
Prof. Teodora P. Popova ◽  
Toshka Petrova ◽  
Ignat Ignatov ◽  
Stoil Karadzhov
Keyword(s):  
Dietary Supplement ◽  
Broad Spectrum ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Clinical Effectiveness ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Microbial Strains

The antimicrobial action of the dietary supplement Oxidal® was tested using the classic Bauer and Kirby agar-gel diffusion method. Clinical and reference strains of Staphylococcus aureus and Escherichia coli were used in the studies. The tested dietary supplement showed a well-pronounced inhibitory effect against the microbial strains commensurable with that of the broad-spectrum chemotherapeutic agent Enrofloxacin and showed even higher activity than the broad spectrum antibiotic Thiamphenicol. The proven inhibitory effect of the tested dietary supplement against the examined pathogenic bacteria is in accordance with the established clinical effectiveness standards for antimicrobial agents.

In-vitro Antioxidant and Antibacterial Activity of Methanolic Extract of Shorea robusta Gaertn. F. Resin

2016 ◽  
Vol 6 (4) ◽  
pp. 68
Author(s):  
Sushma Vashisht ◽  
Manish Pal Singh ◽  
Viney Chawla
Keyword(s):  
Antibacterial Activity ◽  
Methanolic Extract ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Gram Positive ◽  
Gram Negative ◽  
Shorea Robusta ◽  
Dose Dependent ◽  
Resin Extract

The methanolic extract of the resin of Shorea robusta was subjected to investigate its antioxidant and antibacterial properties its utility in free radical mediated diseases including diabetic, cardiovascular, cancer etc. The methanol extract of the resin was tested for antioxidant activity using scavenging activity of DPPH (1,1-diphenyl-2-picrylhydrazil) radical method, reducing power by FeCl3 and antibacterial activity against gram positive and gram negative bacteria using disc diffusion method. The phytochemical screening considered the presence of triterpenoids, tannins and flavoniods. Overall, the plant extract is a source of natural antioxidants which might be helpful in preventing the progress of various oxidative stress mediated diseases including aging. The half inhibition concentration (IC50) of resin extract of Shorea robusta and ascorbic acid were 35.60 µg/ml and 31.91 µg/ml respectively. The resin extract exhibit a significant dose dependent inhibition of DPPH activity. Antibacterial activity was observed against gram positive and gram negative bacteria in dose dependent manner.Key Words: Shorea robusta, antioxidant, antibacterial, Disc-diffusion, DPPH.

Antibacterial Activity of Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa

2017 ◽  
Vol 8 (03) ◽  
Author(s):  
Kyoung- Sun Seo ◽  
Seong Woo Jin ◽  
Seongkyu Choi ◽  
Kyeong Won Yun
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Methanol Extract ◽  
The Other ◽  
Diffusion Method ◽  
E Coli ◽  
Agar Diffusion ◽  
Agar Diffusion Method ◽  
Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

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