scholarly journals Effect of Magnesium on Dentinogenesis of Human Dental Pulp Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Rania M. Salem ◽  
Chang Zhang ◽  
Laisheng Chou
Keyword(s):  
Cell Proliferation ◽  
Dental Pulp ◽  
Cell Attachment ◽  
Proliferation Rate ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Pulp Capping ◽  
Alp Activity ◽  
Human Dental Pulp ◽  
Pulp Cells

Introducing therapeutic ions into pulp capping materials has been considered a new approach for enhancing regeneration of dental tissues. However, no studies have been reported on its dentinogenic effects on human dental pulp cells (HDPCs). This study was designed to investigate the effects of magnesium (Mg2+) on cell attachment efficiency, proliferation, differentiation, and mineralization of HDPCs. HDPCs were cultured with 0.5 mM, 1 mM, 2 mM, 4 mM, and 8 mM concentrations of supplemental Mg2+ and 0 mM (control). Cell attachment was measured at 4, 8, 12, 16, and 20 hours. Cell proliferation rate was evaluated at 3, 7, 10, 14, and 21 days. Crystal violet staining was used to determine cell attachment and proliferation rate. Alkaline phosphatase (ALP) activity was assessed using the fluorometric assay at 7, 10, and 14 days. Mineralization of cultures was measured by Alizarin red staining. Statistical analysis was done using multiway analysis of variance (multiway ANOVA) with Wilks’ lambda test. Higher cell attachment was shown with 0.5 mM and 1 mM at 16 hours compared to control ( P < 0.0001 ). Cells with 0.5 mM and 1 mM supplemental Mg2+ showed significantly higher proliferation rates than control at 7, 10, 14, and 21 days ( P < 0.0001 ). However, cell proliferation rates decreased significantly with 4 mM and 8 mM supplemental Mg2+ at 14 and 21 days ( P < 0.0001 ). Significantly higher levels of ALP activity and mineralization were observed in 0.5 mM, 1 mM, and 2 mM supplemental Mg2+ at 10 and 14 days ( P < 0.0001 ). However, 8 mM supplemental Mg2+ showed lower ALP activity compared to control at 14 days ( P < 0.0001 ), while 4 mM and 8 mM supplemental Mg2+showed less mineralization compared to control ( P < 0.0001 ). The study indicated that the optimal (0.5–2 mM) supplemental Mg2+ concentrations significantly upregulated HDPCs by enhancing cell attachment, proliferation rate, ALP activity, and mineralization. Magnesium-containing biomaterials could be considered for a future novel dental pulp-capping additive in regenerative endodontics.

scholarly journals Effects of different aperture-sized type I collagen/silk fibroin scaffolds on the proliferation and differentiation of human dental pulp cells

2021 ◽  
Vol 8 (4) ◽  
Author(s):  
Shihui Jiang ◽  
Zhaoxia Yu ◽  
Lanrui Zhang ◽  
Guanhua Wang ◽  
Xiaohua Dai ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Dental Pulp ◽  
Type I Collagen ◽  
Type I ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Alp Activity ◽  
Proliferation And Differentiation ◽  
Human Dental Pulp ◽  
Pulp Cells

Abstract This study aimed at evaluate the effects of different aperture-sized type I collagen/silk fibroin (CSF) scaffolds on the proliferation and differentiation of human dental pulp cells (HDPCs). The CSF scaffolds were designed with 3D mapping software Solidworks. Three different aperture-sized scaffolds (CSF1–CSF3) were prepared by low-temperature deposition 3D printing technology. The morphology was observed by scanning electron microscope (SEM) and optical coherence tomography. The porosity, hydrophilicity and mechanical capacity of the scaffold were detected, respectively. HDPCs (third passage, 1 × 105 cells) were seeded into each scaffold and investigated by SEM, CCK-8, alkaline phosphatase (ALP) activity and HE staining. The CSF scaffolds had porous structures with macropores and micropores. The macropore size of CSF1 to CSF3 was 421 ± 27 μm, 579 ± 36 μm and 707 ± 43 μm, respectively. The porosity was 69.8 ± 2.2%, 80.1 ± 2.8% and 86.5 ± 3.3%, respectively. All these scaffolds enhanced the adhesion and proliferation of HDPCs. The ALP activity in the CSF1 group was higher than that in the CSF3 groups (P &lt; 0.01). HE staining showed HDPCs grew in multilayer within the scaffolds. CSF scaffolds significantly improved the adhesion and ALP activity of HDPCs. CSF scaffolds were promising candidates in dentine-pulp complex regeneration.

17β-Estradiol induces odontoblastic differentiation via activation of the c-Src/MAPK pathway in human dental pulp cells

2015 ◽  
Vol 93 (6) ◽  
pp. 587-595 ◽  
Author(s):  
Su Mi Woo ◽  
Kyung Joo Seong ◽  
Sang Jin Oh ◽  
Hong Ju Park ◽  
Sun Hun Kim ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Mapk Pathway ◽  
Mapk Signaling ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Odontoblastic Differentiation ◽  
Alp Activity ◽  
Human Dental Pulp ◽  
Pulp Cells ◽  
17Β Estradiol

The present study is aimed at investigating the effects of the exogenous estrogen 17β-estradiol (E2) on odontoblastic differentiation in human dental pulp cells (HDPCs) immotalized with hTERT gene and their molecular mechanism. Proliferation was detected by BrdU assay, and odontoblast differentiation induction was evaluated by the expression of dentin sialophosphoprotein (DSPP), dentin sialoprotein (DSP) and dentin matrix protein1 (DMP1), and alkaline phosphatase (ALP) activity and mineralization. Estrogen receptor-α (ER-α), c-Src, and mitogen-activated protein kinases (MAPKs) were examined and their inhibitors were used to determine the roles on odontogenic induction. E2 significantly promoted the HDPC proliferation, which was mediated by extracellular signal-related kinase 1/2. E2 upregulated DSPP, DSP, and DMP1 as the odontogenic differentiation markers and enhanced ALP activity and mineralization. E2 increased phosphorylation of ER-α and fulvestrant, an ER downregulator, significantly downregulated DSPP, DMP1, and DSP induced by E2. Moreover, E2 treatment activated c-Src and MAPKs upon odontogenic induction, whereas chemical inhibition of c-Src and MAPKs decreased expression of DSPP, DMP1, and DSP and mineralization augmented by E2. Moreover, fulvestrant reduced E2-induced phosphorylation of c-Src and MAPK and inhibition of c-Src by PP2 attenuated activation of MAPKs during E2-induced odontoblastic differentiation. Taken together, these results indicated that E2 stimulates odontoblastic differentiation of HDPCs via coordinated regulation of ER-α, c-Src, and MAPK signaling pathways, which may play a key role in the regeneration of dentin.

scholarly journals Response of human dental pulp cells to a silver-containing PLGA/TCP-nanofabric as a potential antibacterial regenerative pulp-capping material

2017 ◽  
Vol 17 (1) ◽  
Author(s):  
Barbara Cvikl ◽  
Samuel C. Hess ◽  
Richard J. Miron ◽  
Hermann Agis ◽  
Dieter Bosshardt ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Pulp Capping ◽  
Human Dental Pulp ◽  
Pulp Cells

scholarly journals TOXICITY ANALYSIS OF CRAB SHELL CHITOSAN ARGINYLGLYCYLASPARTIC ACID SCAFFOLD MEMBRANE AND ITS EFFECT ON HUMAN DENTAL PULP CELL VIABILITY

2018 ◽  
Vol 9 ◽  
pp. 147
Author(s):  
Tri Kurnia Dewi ◽  
Mauldina Shabrina ◽  
Dewi Fatmasuniarti ◽  
Lisa Amir ◽  
Erik Idrus
Keyword(s):  
Dental Pulp ◽  
Cell Attachment ◽  
Dental Pulp Cells ◽  
Crab Shell ◽  
Human Dental Pulp Cells ◽  
Enhance Cell Attachment ◽  
Toxicity Analysis ◽  
Dental Pulp Cell ◽  
Human Dental Pulp ◽  
Pulp Cells

Objective: Crab shell chitosan is a biomaterial used for scaffolding. In Indonesia, Badan Tenaga Nuklir Nasional has made a crab shell chitosan arginylglycylaspartic acid (RGD) scaffold membrane. The purpose of adding RGD was to enhance cell attachment to the scaffold. The objective of this research is to analyze the toxicity of crab shell chitosan RGD scaffold membrane on human dental pulp cells and its effect on their viabilityMethods: Human dental pulp cells were cultured for 5 days in Minimum Essential Medium Alpha (α-MEM) complete containing amphotericin B, penicillin, streptomycin, and fetal bovine serum. Then, the treatment group was exposed to crab shell chitosan RGD scaffold membrane and crab shell chitosan scaffold membrane incubated for 24 h. The toxicity of the crab shell chitosan RGD scaffold membrane was analyzed with a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.Result: The result of this research is that crab shell chitosan RGD scaffold membrane did not decrease the percentage of viability of human dental pulp cells.Conclusion: It is concluded that crab shell chitosan RGD scaffold membrane does not have toxic effects on human dental pulp cells.

scholarly journals Cytotoxic Effect of Chitosan Nanoparticles on Normal Human Dental Pulp Cells

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Rami Alhomrany ◽  
Chang Zhang ◽  
Laisheng Chou
Keyword(s):  
Cell Viability ◽  
Dental Pulp ◽  
Cell Attachment ◽  
Chitosan Nanoparticles ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Attachment Efficiency ◽  
Human Dental Pulp ◽  
Pulp Cells ◽  
Normal Human

 Introduction: Recent in vitro studies have shown that chitosan nanoparticles could enhance the antimicrobial activity of several dental materials. However, the biocompatibility of these nanoparticles with normal human cells is still controversial. The aim of this study was to evaluate the potential toxicity of various sizes and concentrations of chitosan nanoparticles cultured with normal human dental pulp cells. Methods: Normal human dental pulp cells were derived from human dental pulp tissues and cultured with (50-67) nm and (318-350) nm chitosan nanoparticles in concentrations: 0.2 mg/mL, 0.5 mg/mL, 1 mg/mL, and 2 mg/mL as study groups, and 0 mg/mL as a control. The cell attachment efficiency for each group was assessed at 16 hours. The proliferation rate and cell viability were evaluated at days 7 and 14. Both, attachment efficiency and proliferation rate were assessed by measuring the optical density of crystal violet stained cells. The cell viability was determined by the activity of the mitochondrial dehydrogenase enzyme. Statistical analysis was performed using One-Way ANOVA and post hoc Tukey test. Results: All concentrations of the (50-67) nm group significantly reduced cell attachment efficiency in comparison with the control (p<0.01) and with the (318-350) nm group (p<0.01). All concentrations of both groups, (50-67) nm and (318-350) nm, significantly reduced cell proliferation and cell viability compared to the control in dose-dependent and size-associated manners. (p<0.01).    Conclusion: Chitosan nanoparticles exhibit a cytotoxic effect on normal human dental pulp cells

Odontogenic differentiation of human dental pulp cells induced by silicate-based bioceramics via activation of P38/MEPE pathway

RSC Advances ◽  
2015 ◽  
Vol 5 (89) ◽  
pp. 72536-72543 ◽  
Author(s):  
Xiaomeng Zhang ◽  
Chengtie Wu ◽  
Jiang Chang ◽  
Jiao Sun
Keyword(s):  
Protein Expression ◽  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Alp Activity ◽  
Odontogenic Differentiation ◽  
Human Dental Pulp ◽  
Pulp Cells

Ca7Si2P2O16 bioceramic significantly enhanced odontogenic protein expression (ALP activity and staining) of hDPCs.

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