Odontogenic differentiation of human dental pulp cells induced by silicate-based bioceramics via activation of P38/MEPE pathway

RSC Advances ◽  
2015 ◽  
Vol 5 (89) ◽  
pp. 72536-72543 ◽  
Author(s):  
Xiaomeng Zhang ◽  
Chengtie Wu ◽  
Jiang Chang ◽  
Jiao Sun
Keyword(s):  
Protein Expression ◽  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Alp Activity ◽  
Odontogenic Differentiation ◽  
Human Dental Pulp ◽  
Pulp Cells

Ca7Si2P2O16 bioceramic significantly enhanced odontogenic protein expression (ALP activity and staining) of hDPCs.

scholarly journals Effects of different aperture-sized type I collagen/silk fibroin scaffolds on the proliferation and differentiation of human dental pulp cells

2021 ◽  
Vol 8 (4) ◽  
Author(s):  
Shihui Jiang ◽  
Zhaoxia Yu ◽  
Lanrui Zhang ◽  
Guanhua Wang ◽  
Xiaohua Dai ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Dental Pulp ◽  
Type I Collagen ◽  
Type I ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Alp Activity ◽  
Proliferation And Differentiation ◽  
Human Dental Pulp ◽  
Pulp Cells

Abstract This study aimed at evaluate the effects of different aperture-sized type I collagen/silk fibroin (CSF) scaffolds on the proliferation and differentiation of human dental pulp cells (HDPCs). The CSF scaffolds were designed with 3D mapping software Solidworks. Three different aperture-sized scaffolds (CSF1–CSF3) were prepared by low-temperature deposition 3D printing technology. The morphology was observed by scanning electron microscope (SEM) and optical coherence tomography. The porosity, hydrophilicity and mechanical capacity of the scaffold were detected, respectively. HDPCs (third passage, 1 × 105 cells) were seeded into each scaffold and investigated by SEM, CCK-8, alkaline phosphatase (ALP) activity and HE staining. The CSF scaffolds had porous structures with macropores and micropores. The macropore size of CSF1 to CSF3 was 421 ± 27 μm, 579 ± 36 μm and 707 ± 43 μm, respectively. The porosity was 69.8 ± 2.2%, 80.1 ± 2.8% and 86.5 ± 3.3%, respectively. All these scaffolds enhanced the adhesion and proliferation of HDPCs. The ALP activity in the CSF1 group was higher than that in the CSF3 groups (P < 0.01). HE staining showed HDPCs grew in multilayer within the scaffolds. CSF scaffolds significantly improved the adhesion and ALP activity of HDPCs. CSF scaffolds were promising candidates in dentine-pulp complex regeneration.

scholarly journals Melatonin-induced suppression of DNA methylation promotes odontogenic differentiation in human dental pulp cells

Bioengineered ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 829-840
Author(s):  
Jingzhou Li ◽  
Qianyi Deng ◽  
Wenguo Fan ◽  
Qi Zeng ◽  
Hongwen He ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Odontogenic Differentiation ◽  
Human Dental Pulp ◽  
Pulp Cells

17β-Estradiol induces odontoblastic differentiation via activation of the c-Src/MAPK pathway in human dental pulp cells

2015 ◽  
Vol 93 (6) ◽  
pp. 587-595 ◽  
Author(s):  
Su Mi Woo ◽  
Kyung Joo Seong ◽  
Sang Jin Oh ◽  
Hong Ju Park ◽  
Sun Hun Kim ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Mapk Pathway ◽  
Mapk Signaling ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Odontoblastic Differentiation ◽  
Alp Activity ◽  
Human Dental Pulp ◽  
Pulp Cells ◽  
17Β Estradiol

The present study is aimed at investigating the effects of the exogenous estrogen 17β-estradiol (E2) on odontoblastic differentiation in human dental pulp cells (HDPCs) immotalized with hTERT gene and their molecular mechanism. Proliferation was detected by BrdU assay, and odontoblast differentiation induction was evaluated by the expression of dentin sialophosphoprotein (DSPP), dentin sialoprotein (DSP) and dentin matrix protein1 (DMP1), and alkaline phosphatase (ALP) activity and mineralization. Estrogen receptor-α (ER-α), c-Src, and mitogen-activated protein kinases (MAPKs) were examined and their inhibitors were used to determine the roles on odontogenic induction. E2 significantly promoted the HDPC proliferation, which was mediated by extracellular signal-related kinase 1/2. E2 upregulated DSPP, DSP, and DMP1 as the odontogenic differentiation markers and enhanced ALP activity and mineralization. E2 increased phosphorylation of ER-α and fulvestrant, an ER downregulator, significantly downregulated DSPP, DMP1, and DSP induced by E2. Moreover, E2 treatment activated c-Src and MAPKs upon odontogenic induction, whereas chemical inhibition of c-Src and MAPKs decreased expression of DSPP, DMP1, and DSP and mineralization augmented by E2. Moreover, fulvestrant reduced E2-induced phosphorylation of c-Src and MAPK and inhibition of c-Src by PP2 attenuated activation of MAPKs during E2-induced odontoblastic differentiation. Taken together, these results indicated that E2 stimulates odontoblastic differentiation of HDPCs via coordinated regulation of ER-α, c-Src, and MAPK signaling pathways, which may play a key role in the regeneration of dentin.

Biosilica Porous Microspheres Promote the Osteogenic/Odontogenic Differentiation of Human Dental Pulp Cells

2018 ◽  
Vol 8 (2) ◽  
pp. 258-266 ◽  
Author(s):  
Wenhao Zhu ◽  
Xuejun Gao ◽  
Xiaoying Zou ◽  
Werner E. G. Müller ◽  
Shunfeng Wang ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Porous Microspheres ◽  
Odontogenic Differentiation ◽  
Human Dental Pulp ◽  
Pulp Cells

Effect of 5-Aza-2′-deoxycytidine on Odontogenic Differentiation of Human Dental Pulp Cells

2015 ◽  
Vol 41 (5) ◽  
pp. 640-645 ◽  
Author(s):  
Deqian Zhang ◽  
Qimeng Li ◽  
Lijia Rao ◽  
Baicheng Yi ◽  
Qiong Xu
Keyword(s):  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Odontogenic Differentiation ◽  
Human Dental Pulp ◽  
Pulp Cells

scholarly journals Effect of histone demethylase KDM5A on the odontogenic differentiation of human dental pulp cells

Bioengineered ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 449-462 ◽  
Author(s):  
Qi-Meng Li ◽  
Jin-Ling Li ◽  
Zhi-Hui Feng ◽  
Huan-Cai Lin ◽  
Qiong Xu
Keyword(s):  
Dental Pulp ◽  
Histone Demethylase ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Odontogenic Differentiation ◽  
Human Dental Pulp ◽  
Pulp Cells
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