scholarly journals Association of Apolipoprotein E Gene Polymorphism with Lipid Profile and Ischemic Stroke Risk in Type 2 Diabetes Mellitus Patients

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Ni Putu Tesi Maratni ◽  
Made Ratna Saraswati ◽  
Ni Nyoman Ayu Dewi ◽  
I Wayan Putu Sutirta Yasa ◽  
I Putu Eka Widyadharma ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Gene Polymorphism ◽  
Apolipoprotein E ◽  
Density Lipoprotein ◽  
Apoe Gene ◽  
Apolipoprotein E Gene ◽  
Apoe Gene Polymorphism

Background. Altered lipid profiles have consistently been linked to cerebrovascular events. Ischemic stroke (IS) was a common comorbid condition established in type 2 diabetes mellitus (T2DM). The apolipoprotein E (ApoE) gene which has a notably critical function in lipoprotein metabolism is believed as one of the potential candidate genes susceptible to IS complications in T2DM. This research aimed to determine the association of apolipoprotein E gene polymorphism with lipid profile and IS risk in T2DM patients. Methods. This case-control study involved a total of 60 diabetic participants divided into two groups with and without IS. ApoE was genotyped using PCR and sequencing analysis. Results. The most predominant genotype observed in 27 participants (45%) was E3/E3. Lower levels of high-density lipoprotein cholesterol (HDL-C) were found in ε2 carriers ( p = 0.003 ; 95% CI −23.35–−4.89) and ε4 carriers ( p = 0.019 ; 95% CI 1.38–14.55) compared to ε3 homozygotes. Total cholesterol (TC), triglyceride, and low-density lipoprotein cholesterol (LDL-C) levels had no association with ApoE gene polymorphism in this study. ApoE gene polymorphism was not related to IS in T2DM ( p = 0.06 ; adjusted OR: 4.71; 95% CI 0.93–23.79). Conclusions. ApoE ε2 and ε4 carriers were associated with lower levels of HDL-C. No association was identified between ApoE gene polymorphism and IS in T2DM patients.

Relationship Between the ApoE Gene Polymorphism and Type 2 Diabetes Mellitus Complications

2021 ◽  
Vol 25 (2) ◽  
pp. 111-115
Author(s):  
Chang Gao ◽  
Xiaoling Fu ◽  
Qinying Chu ◽  
Jianhua Li ◽  
GangMing Shu
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Gene Polymorphism ◽  
Apoe Gene ◽  
Apoe Gene Polymorphism

Transcription Factor 7-Like-2 (TCF7L2) rs7903146 (C/T) Polymorphism in Patients with Type 2 Diabetes Mellitus

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Arig Aly Seif ◽  
Amira Ibrahim Hamed ◽  
Ahmed Mohamed Bahaaeldin ◽  
Walaa Ahmed Yousry Kabiel ◽  
Mohamed Ahmed Abo El Azm
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Transcription Factor ◽  
High Risk ◽  
Gene Polymorphism ◽  
Density Lipoprotein ◽  
Lipoprotein Cholesterol ◽  
Tcf7l2 Rs7903146

Abstract Background Type 2 diabetes mellitus (T2DM) is a heterogeneous group of metabolic disorders characterized by the incapability of pancreatic beta cells to increase insulin secretion to compensate for insulin resistance in the peripheral tissues. T2DM is a multi-factorial disease including several environmental factors with the presence of genetic predisposition. The transcription factor 7-like-2 gene (TCF7L2) rs7903146 (C/T) polymorphism is one of the most susceptible genes to T2DM discovered to date, with contribution to the disease through the Wnt/β –catenin signaling pathway affecting pancreatic islet development, expression of several genes involved in insulin granules exocytosis and the incretin glucagon-like peptide 1 (GLP-1) gene. Aim of the Work In this study we aimed to investigate the potential association of the transcription factor 7-like-2 (TCF7L2) rs7903146 (C/T) gene polymorphism in patients with type 2 diabetes mellitus. Patients and Methods The study was a case- control study conducted on 70 T2DM patients recruited from the endocrinology clinic at Ain Shams University Hospitals, and 30 non diabetic healthy controls matched with the patients in age and sex. All subjects underwent full history taking, thorough clinical examination, routine laboratory investigations including haemoglobin A1c (HbA1c), lipid profile; total cholesterol (TC), triglycerides (TG), high density lipoprotein-cholesterol (HDL-C) and low density lipoprotein-cholesterol (LDL-C) and determination of TCF7L2 gene polymorphism by real-time quantitative polymerase chain reaction (RT-PCR). Results The minor T allele of the rs7903146(C/T) SNP was associated with high risk of development of T2DM with an OR of 1.35 (95% CI: 0.68-2.6), the heterozygous genotype (CT) with an OR 1.16 (95% CI: 0.49-2.7) and the homozygous mutant genotype (TT) with OR of 3.16 (95% CI: 0.15-6.31), however, they were statistically insignificant (p-value >0.05). Conclusion Our study did not confirm the presence of significant association between the TCF7L2 rs7903146(C/T) polymorphism and T2DM, however, it pointed to the possibility of presence of high risk of development of T2DM in patients with TT genotype. Further studies with higher sample size are needed to clarify the association.

scholarly journals Transcription Factor 7-Like-2 (TCF7L2) rs7903146 (C/T) Polymorphism in Patients with Type 2 Diabetes Mellitus

2020 ◽  
Vol 26 (3) ◽  
pp. 112-118
Author(s):  
Ahmed Mohamed Bahaaeldin ◽  
Arig Aly Seif ◽  
Amira Ibrahim Hamed ◽  
Walaa Ahmed Yousry Kabiel
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Transcription Factor ◽  
High Risk ◽  
Gene Polymorphism ◽  
Density Lipoprotein ◽  
Lipoprotein Cholesterol ◽  
Peripheral Tissues

<b><i>Introduction:</i></b> Type 2 diabetes mellitus (T2DM) is a metabolic disorder characterized by the incapability of pancreas to increase insulin secretion to compensate for insulin resistance in the peripheral tissues. T2DM is a multifactorial disease including several environmental factors with the presence of genetic predisposition. The transcription factor 7-like-2 gene (<i>TCF7L2</i>) rs7903146 (C/T) polymorphism is one of the most susceptible genes to T2DM discovered to date, with contribution to the disease through the Wnt/β-catenin signaling pathway affecting pancreatic islet development, expression of several genes involved in insulin granules exocytosis, and the incretin glucagon-like peptide 1 (<i>GLP-1</i>) gene. Then, <i>TCF7L2</i> gene seems to affect diabetes susceptibility through B-cell dysfunction that is why we studied its association with T2DM in particular. <b><i>Objectives:</i></b> To investigate the potential association of the transcription factor 7-like-2 (<i>TCF7L2</i>) rs7903146 (C/T) gene polymorphism in patients with T2DM. <b><i>Methods:</i></b> A case-control study conducted on 70 T2DM patients recruited from the endocrinology clinic at Ain Shams University Hospitals, and 30 non-diabetic healthy controls age- and sex-matched with the patients. All subjects underwent full history taking; thorough clinical examination; routine laboratory investigations including hemoglobin A1c, total cholesterol, triglycerides, high-density lipoprotein-cholesterol, and low-density lipoprotein-cholesterol; and determination of <i>TCF7L2</i> gene polymorphism by qRT-PCR. <b><i>Results:</i></b> The minor T allele of the rs7903146(C/T) SNP was associated with high risk of development of T2DM with an OR of 1.35 (95% CI: 0.68–2.6) and the heterozygous genotype (CT) with an OR 1.16 (95% CI: 0.49–2.7); however, they were statistically insignificant (<i>p</i> value &#x3e;0.05). <b><i>Conclusion:</i></b> Our study did not confirm the presence of significant association between the <i>TCF7L2</i> rs7903146(C/T) polymorphism and T2DM; however, it pointed out the possibility of presence of high risk of development of T2DM in patients with TT genotype. Further studies with higher sample size are needed to clarify the association.

Correlation between TNF-α- serum level and TNF-α -308 gene polymorphism in Iraqi patients with type 2 diabetes mellitus

Gene Reports ◽  
2021 ◽  
Vol 23 ◽  
pp. 101136
Author(s):  
Sarah Rahman Rasool ◽  
Othman Taha Qasim ◽  
Salaam Khudhur Muslem ◽  
Muataz Mohammed Al-Taee
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Serum Level ◽  
Gene Polymorphism ◽  
Tnf Α
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