scholarly journals Antioxidant Activities of Commiphora leptophloeos (Mart.) J. B. Gillett) (Burseraceae) Leaf Extracts Using In Vitro and In Vivo Assays

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Maria Lúcia da Silva Cordeiro ◽  
Ana Raquel Carneiro Ribeiro ◽  
Luciana Fentanes Moura de Melo ◽  
Lucas Felipe da Silva ◽  
Gabriel Pereira Fidelis ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging ◽  
In Vitro Assays ◽  
Leaf Extracts ◽  
In Vivo Assays

Commiphora leptophloeos is widely used in folk medicine without any scientific basis. Considering this, the aim of this study was to evaluate the chemical profile and the antioxidant activity of C. leptophloeos leaf extracts using in vitro and in vivo assays. Six extracts were obtained from fresh leaves using a serial extraction (nonpolar to polar solvents). These extracts were first evaluated with the presence of phytochemical compounds using the methods thin layer chromatography (TLC), ultrahigh performance liquid chromatography (UHPLC-DAD), and high performance liquid chromatography, both with diode array detection (HPLC-DAD). Based on the compounds identified, it was used some bioinformatics tools in order to identify possible pathway and gene targets. After that, the antioxidant capacity from these extracts was analysed by in vitro assays and in vivo assays using Caenorhabditis elegans model. Phytochemical analyses showed the presence of polyphenols, such as rutin, vitexin, and quercetin diglycosides in all extracts, especially in ethanol extract (EE) and methanol extract (EM). Bioinformatics analysis showed these polyphenols linked to antioxidant pathways. Furthermore, EE and EM displayed a high antioxidant capacity in DPPH and superoxide radical scavenging assays. They also had no effect on cell viability for 3T3 nontumour cell. However, for B16-F10 tumour cell lines, these extracts had toxicity effect. In vivo assays using C. elegans N2 showed that EE was not toxic, and it did not affect its viability nor its development. Besides, EE increased worm survival under oxidative stress and reduced intracellular reactive oxygen species (ROS) levels by 50%. Thus, C. leptophloeos EE displayed an important in vitro and in vivo antioxidant capacity. The EE extract has polyphenols, suggesting that these compounds may be responsible for a myriad of biological activities having this potential to be used in various biotechnological applications.

scholarly journals IN VITRO ANTIOXIDANT POTENTIALS AND HEPATOPROTECTIVE EFFECTS OF Asystasia vogeliana (BENTH) LEAF EXTRACTS AGAINST PARACETAMOL-INDUCED HEPATIC INJURY IN RATS

2020 ◽  
Vol 8 (6) ◽  
pp. 839-848
Author(s):  
Emeka Hillary Ugwuanyi ◽  
◽  
Chukwuneke Udem Samuel ◽  
Ifeanyi Innocent Madubuinyi ◽  
◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging ◽  
In Vitro Assays ◽  
Distilled Water ◽  
Leaf Extracts ◽  
Group V ◽  
Reference Drug ◽  
Group I

This study was aimed to investigate the antioxidant potentials of methanol and petroleum ether leaf extracts of Asystasia vogeliana against paracetamol-induced liver injury in rats. For estimation of antioxidant potentials, in vitro radical scavenging assays were carried out using DPPH, FRAP, and ABTS. For in vivo study, twenty-five male Wistar rats weighing 100-120 g were randomized and assigned into 5 groups (I-V, n=5). Further, Paracetamol (PCM) at 2 g/kg was used to induce acute hepatotoxicity orally. Rats in group I received distilled water (10 ml/kg) only. While, the rats of groups II, III, and IV received MLEAV (200 mg/kg), PLEAV (200 mg/kg), and a standard hepatoprotective reference drug silymarin (25 mg/kg) respectively for 5 days before PCM induction. Rats in group V received distilled water for 5 days before PCM induction. Blood and liver samples were collected for hematology, serum biochemistry, and histopathology analyses using standard procedures. In vitro assays revealed that MLEAV showed significant (P < 0.05) increases in antioxidant activity compared with PLEAV. Further, significant (P < 0.05) reductions in the activities of ALT and ALP while a significant (P < 0.05) increases in the activity of antioxidant enzymes (CAT, SOD, and GPx) were reported in the group II and III compared with group V. There were also no observable lesions in their hepatocytes. Results of the study can be concluded that MLEAV elicited more in vitro and in vivo antioxidant activities than PLEAV, thus it protects the liver of rat from PCM-induced hepatotoxicity. Therefore, MLEAV could be used as a hepatoprotective agent for the clinical management of liver damage.

scholarly journals Antioxidant Activities and Reduced Amyloid-β Toxicity of 7-Hydroxycalamenene Isolated from the Essential Oil of Zelkova serrata Heartwood

2016 ◽  
Vol 11 (9) ◽  
pp. 1934578X1601100
Author(s):  
Pei-Ling Yen ◽  
Sen-Sung Cheng ◽  
Chia-Cheng Wei ◽  
Huan-You Lin ◽  
Vivian Hsiu-Chuan Liao ◽  
...  
Keyword(s):  
Essential Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Amyloid Β ◽  
Total Phenolic ◽  
C Elegans ◽  
In Vivo Assays ◽  
Zelkova Serrata

The in vitro and in vivo antioxidant activities and its potential to protect against amyloid-β toxicity of essential oils from Zelkova serrata (Thunb.) Makino were investigated in the model organism Caenorhabditis elegans. The results revealed that the essential oil of Z. serrata heartwood exhibited great radical scavenging activities and high total phenolic content. In vivo assays showed significant inhibition of oxidative damage in wild-type C. elegans under juglone-induced oxidative stress and heat shock. Based on results from both in vitro and in vivo assays, the major compound in essential oil of heartwood, (-)-(1 S, 4 S)-7-hydroxycalamenene (1 S, 4 S-7HC), may contribute significantly to the observed antioxidant activity. Further evidence showed that 1 S, 4 S-7HC significantly delayed the paralysis phenotype in amyloid beta-expressing transgenic C. elegans. These findings suggest that 1 S, 4 S-7HC from the essential oil of Z. serrata heartwood has potential as a source for antioxidant or Alzheimer's disease treatment.

scholarly journals Physicochemical Characterization and Biological Activities of Black and White Garlic: In Vivo and In Vitro Assays

Foods ◽  
2019 ◽  
Vol 8 (6) ◽  
pp. 220 ◽  
Author(s):  
María Ángeles Toledano Medina ◽  
Tania Merinas-Amo ◽  
Zahira Fernández-Bedmar ◽  
Rafael Font ◽  
Mercedes del Río-Celestino ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Genetic Model ◽  
Biological Activities ◽  
Leukemia Cell ◽  
Physicochemical Characterization ◽  
Biological Data ◽  
In Vitro Assays ◽  
Age Related

White and three types of black garlic (13, 32, and 45 days of aging, named 0C1, 1C2, and 2C1, respectively) were selected to study possible differences in their nutraceutic potential. For this purpose, garlic were physicochemically characterized (Brix, pH, aW, L, polyphenol, and antioxidant capacity), and both in vivo and in vitro assays were carried out. Black garlic samples showed higher polyphenol content and antioxidant capacity than the white ones. The biological assays showed that none of the samples (neither raw nor black garlic) produced toxic effects in the Drosophila melanogaster animal genetic model, nor exerted protective effects against H2O2, with the exception of the 0C1 black garlic. Moreover, only white garlic was genotoxic at the highest concentration. On the other hand, 0C1 black garlic was the most antigenotoxic substance. The in vivo longevity assays showed significant extension of lifespan at some concentrations of white and 0C1and 1C2 black garlic. The in vitro experiments showed that all of the garlic samples induced a decrease in leukemia cell growth. However, no type of garlic was able to induce proapoptotic internucleosomal DNA fragmentation. Taking into account the physicochemical and biological data, black garlic could be considered a potential functional food and used in the preventive treatment of age-related diseases. In addition, our findings could be relevant for black-garlic-processing agrifood companies, as the economical and timing costs can significantly be shortened from 45 to 13 days of aging.

scholarly journals Potential In Vitro and In Vivo Antioxidant Activities from Piper Crocatum and Persea Americana Leaf Extracts

2019 ◽  
Vol 12 (2) ◽  
pp. 661-667
Author(s):  
Fatmawaty Fatmawaty ◽  
Ni G. M. Anggreni ◽  
Naufal Fadhil ◽  
Vivitri D. Prasasty
Keyword(s):  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Persea Americana ◽  
Health Claims ◽  
In Vitro Assays ◽  
Moderate Activity ◽  
Leaf Extracts ◽  
Water Fraction

Piper crocatum and Persea americana Mill leaves are commonly used in traditional medicinal remedies, such as antidiabetes, antitumors and Alzheimer treatment. However, the extensive use of plant extracts over worldwide becomes significant concerns including its safety, efficacy, and quality. Therefore, the accurate scientific evaluation has become a precondition for acceptance of herbal health claims. The aim of this study was to determine the antioxidant activities of Piper crocatum Ruiz & Pav and Persea americana Mill leaf extracts. Isolation of antioxidant fractions were conducted using organic solvent extraction techniques. Antioxidant assays were conducted by using in vitro and in vivo methods involving DPPH and MDA methods. In this study, in vitro assays of Persea americana Mill and Piper crocatum Ruiz & Pav leaf extracts showed the best activity in water fraction. Moreover, in vivo assays of both plant leaf extracts showed the best dose at 8 mg. Persea americana Mill and Piper crocatum Ruiz & Pav leaf extracts have been successfully determined in antioxidant actions in vitro and in vivo. Persea americana Mill in water and ethanol solvents exhibit strong antioxidant properties. Meanwile, Piper crocatum Ruiz & Pav exhibit moderate activity in water and weak antioxidant activity in ethanol. Both plant leaves showed that 8 mg dose was better than the dose of 4 mg and 16 mg in vivo.

scholarly journals Antioxidant ability of polyphenols from black rice, buckwheat and oats: In vitro and in vivo

2020 ◽  
Vol 38 (No. 4) ◽  
pp. 242-247
Author(s):  
Fengying Xie ◽  
Yuchen Lei ◽  
Xue Han ◽  
Yuying Zhao ◽  
Shuang Zhang
Keyword(s):  
Antioxidant Capacity ◽  
Intestinal Tract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antioxidant Ability ◽  
Black Rice ◽  
Total Antioxidant ◽  
Staple Foods

Grains (black rice, buckwheat and oats) contain polyphenols and have stronger antioxidant capacity than staple foods. Their polyphenols were identified and investigated for their antioxidant capacity. The black rice and buckwheat polyphenols were mainly flavonoids; those in oats were phenolic acids. In vitro, their radical-scavenging capacities were determined as black rice &gt; buckwheat &gt; oats. Similarly, in vivo, the increase in total antioxidant capacities and decline in malondialdehyde indicated the enhancement of radical-scavenging and repair abilities of all polyphenol extracts. Differences in superoxide dismutase, catalase activities, glutathione peroxidase activities and oxidase activities suggested that polyphenols from black rice and buckwheat have higher antioxidant activities, indicating that their antioxidant ability is related to polyphenol composition which depends on a polyphenol source. Thus, a combination of diets will make a complementary mixture of polyphenols that can enhance absorption in the intestinal tract and defence ability against oxidative stress.

scholarly journals Scrophularia Tenuipes Coss and Durieu: Phytochemical Composition and Biological Activities

Molecules ◽  
2020 ◽  
Vol 25 (7) ◽  
pp. 1647
Author(s):  
Zeyneb Chaibeddra ◽  
Salah Akkal ◽  
Houria Ouled-Haddar ◽  
Artur M. S. Silva ◽  
Ammar Zellagui ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Health Benefit ◽  
Total Phenolic ◽  
Phytochemical Composition ◽  
Rat Paw Edema ◽  
Anti Inflammatory

Scrophularia tenuipes is an Algerian-Tunisian endemic species, which has not been studied yet. Ethyl acetate (EA) and n-butanol (Bu) fractions obtained from Scrophularia tenuipes were investigated for their health benefit properties, in particular with respect to in vivo/in vitro anti-inflammatory and antioxidant activities, as well as their potential to inhibit key enzymes with impact in diabetes (α-glucosidase and α-amylase). The fractions had a distinct phytochemical composition, of which EA was richer in total phenolic compounds (225 mg GAE/g) and mostly composed of the phenylethanoid acetyl martynoside. Compared to EA, Bu had higher amounts of total flavonoids, and according to the result obtained from UHPLC-DAD-ESI-MSn analysis, harpagoside (iridoid) was its major phytochemical. EA fraction was quite promising with regard to the in vivo (at 200 mg/kg, po) anti-inflammatory effect (62% and 52% for carrageenan-induced rat paw edema and xylene-induced ear edema tests, respectively), while Bu fraction exhibited a stronger antioxidant capacity in all tests (IC50 = 68 µg/mL, IC50 = 18 µg/mL, IC50 = 18 µg/mL and A0.50 = 43 µg/mL for DPPH●, ABTS•+, O2•− scavenging assays and cupric-reducing antioxidant capacity method, respectively). Both fractions also showed a strong effect against α-amylase enzyme (IC50 = 8 µg/mL and 10 µg/mL for EA and Bu fraction, respectively).

scholarly journals Isolation of Strong Antioxidants from Paeonia Officinalis Roots and Leaves and Evaluation of Their Bioactivities

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 249 ◽  
Author(s):  
Lijana Dienaitė ◽  
Milda Pukalskienė ◽  
Audrius Pukalskas ◽  
Carolina V. Pereira ◽  
Ana A. Matias ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Hydroxyl Radical ◽  
Antioxidant Capacity ◽  
High Performance ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Water Extract ◽  
Dependent Manner ◽  
Leaf Extracts

Paeonia officinalis extracts from leaves and roots were tested for their antioxidant potential using in vitro chemical (Folin-Ciocalteu, 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), oxygen radical absorbance capacity (ORAC), hydroxyl radical antioxidant capacity (HORAC), hydroxyl radical scavenging capacity HOSC)) and cellular antioxidant activity (CAA) assays. Leaf extracts were stronger antioxidants than root extracts, while methanol was a more effective solvent than water in chemical assays. However, the selected water extract of leaves was a stronger antioxidant in CAA than the methanol extract (0.106 vs. 0.046 µmol quercetin equivalents/mg). Twenty compounds were identified by ultra performance liquid chromatography-quadrupole-time-of-flight (UPLC-Q-TOF) mass spectrometer, while on-line screening of their antioxidant capacity by high performance liquid chromatography (HPLC) with a DPPH•-scavenging detector revealed that gallic acid derivatives are the major peony antioxidants. Root water and leaf methanol extracts inhibited α-amylase in a dose dependent manner. The IC50 value for the strongest inhibitor, the methanol extract of leaves, was 1.67 mg/mL. In addition, the cytotoxicity assessment of extracts using human Caco-2 cells demonstrated that none of them possessed cytotoxic effects.

scholarly journals 2-Oxo-Imidazole-Containing Dipeptides Play a Key Role in the Antioxidant Capacity of Imidazole-Containing Dipeptides

Antioxidants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1434
Author(s):  
Shingo Kasamatsu ◽  
Somei Komae ◽  
Kana Matsukura ◽  
Yuki Kakihana ◽  
Koji Uchida ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
High Performance ◽  
Radical Scavenging ◽  
In Vitro Assays ◽  
Dpph Radical ◽  
Antioxidant Power ◽  
Mouse Tissues ◽  
Dpph Radical Scavenging

There is substantial evidence for the antioxidant functions of imidazole-containing dipeptides (IDPs), including carnosine and anserine, under physiological and pathological conditions in vivo. However, the detailed mechanism underlying the antioxidant functions is still poorly understood. Recently, we discovered the endogenous production of 2-oxo-imidazole-containing dipeptides (2-oxo-IDPs), such as 2-oxo-carnosine and 2-oxo-anserine, as novel derivatives of IDPs in mouse tissues and revealed that the antioxidant capacity of 2-oxo-carnosine was much greater than that of carnosine. However, the antioxidant capacity of 2-oxo-IDPs still remains unclear. In this study, we evaluated 2-oxo-carnosine and 2-oxo-anserine by multiple in vitro assays, such as 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing/antioxidant power, and oxygen radical absorbance capacity assays in comparison with the corresponding IDPs, carnosine and anserine. All the assays employed herein demonstrated that 2-oxo-carnosine and 2-oxo-anserine exhibited a greater antioxidant capacity than that of the corresponding IDPs. Quantitative high-performance liquid chromatography tandem mass spectrometry revealed that commercial IDPs standards were contaminated with a certain amount of 2-oxo-IDPs, which was correlated with the antioxidant capacity. DPPH radical scavenging assay revealed that the elimination of contaminated 2-oxo-IDPs from the IDPs standards caused a significant decrease in the antioxidant capacity compared to the original IDPs standards. These results suggest that the main driver of the antioxidant capacity of IDPs is 2-oxo-IDPs; accordingly, the conversion of IDPs to 2-oxo-IDPs may be a critical step in the antioxidant functions.

scholarly journals In Vitro Antioxidant and In Vivo Antidiabetic Properties of Citrus Maxima Leaf Extracts in Alloxan-Induced Swiss Albino Diabetic Mice

2021 ◽  
pp. 66-79
Author(s):  
Ariful Islam ◽  
Most. Naoshia Tasnin ◽  
Md. Wasim Bari ◽  
Md. Ismail Hossain ◽  
Mohammad Amirul Islam
Keyword(s):  
Free Radical ◽  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Diabetic Mellitus ◽  
Leaf Extracts ◽  
Citrus Maxima ◽  
Free Radical Scavenging Assay

Aims: The current study aimed to explore in vitro antioxidant capacity and in vivo antidiabetic property of Citrus maxima leaf. Methods: Citrus maxima leaf extracts were prepared using methanol (MECML) and ethanol (EECML) in this study. Antioxidant capacity of both extracts was evaluated using total antioxidant capacity (TAC) assay, ferric reducing antioxidant power (FRAP) assay, DPPH free radical scavenging assay, and ABTS free radical scavenging assay. Cytotoxicity of MECML and EECML was assessed by brine shrimp lethality bioassay. To explore the in vivo antidiabetic property of MECML and EECML, diabetic mellitus (DM) was induced in Swiss albino mice by a single intraperitoneal injection of alloxan. Then diabetic mice were treated with both extracts for 28 days. Effects of both extracts on serum levels of glucose, liver function enzymes, and parameters of lipid profile associated with DM were evaluated. Results: In TAC and FRAP assays, MECML and EECML represented gradually increased reducing capacity in a dose-dependent manner. In DPPH and ABTS assays, both extracts showed notable free radical scavenging activity with lower IC50 values. Additionally, MECML and EECML showed very low toxicity with LC50 values of 80.46 and 105.59 μg/mL, respectively in brine shrimp lethality assay. Moreover, both extracts significantly augmented altered levels of serum glucose, parameters of lipid profile, SGPT, SGOT, and C-reactive protein with the treatment of MECML and EECML.  Conclusion: This study suggests Citrus maxima leaf possesses significant antioxidant and antidiabetic properties and they might play a potential role to prevent diabetic mellitus and diabetic mellitus associated complications.

scholarly journals In Vitro and In Vivo Antioxidant Potential of Hydromethanolic Extract of Phoenix dactylifera Fruits

2009 ◽  
Vol 2 (1) ◽  
pp. 144-157 ◽  
Author(s):  
S. Naskar ◽  
A. Islam ◽  
U. K. Mazumder ◽  
P. Saha ◽  
P. K. Haldar ◽  
...  
Keyword(s):  
Vitamin C ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Phoenix Dactylifera ◽  
In Vivo Studies ◽  
In Vitro Assays ◽  
Total Phenolic ◽  
Serum Glutamate

The present study was aimed at investigating the antioxidant activities of the hydromethanolic extract of Phoenix dactylifera (HEPD) fruit (Arecaceae). The antioxidant activities of extract have been evaluated by using a range of in vitro assays and in vivo hepatoprotective model. In case of in vitro studies the IC50 values were found to be 160, 1400, 1115, 1050 μg/ml in DPPH, nitric oxide, superoxide, hydroxyl radical scavenging assays, respectively. In case of in vivo studies the levels of liver enzymatic, non-enzymatic systems [serum glutamate oxalo-acetate transaminases (SGOT), serum glutamate pyruvate transaminases (SGPT), alkaline phosphatase (ALP), total bilirubin, total protein, catalase (CAT), glutathione (GSH), superoxide dismutase (SOD)] and lipid peroxidation (LPO) were restored towards the normal value in HEPD treated carbon tetrachloride intoxicated rats. The free radical scavenging and antioxidant activities may be attributed to the presence of phenolic (pyrocatechol and gallic acid contents are 6.2 and 2.906 μg/mg, respectively), vitamin C (ascorbic acid content is 0.66 μg/mg) and flavonoid compounds (4.79 μg/mg) present in HEPD. The results obtained in the present study indicate that the Phoenix dactylifera fruit is a potential source of natural antioxidant.  Keywords: Phoenix dactylifera; Antioxidant, Flavonoid; Total phenolic content; Vitamin C. © 2010  JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.  DOI: 10.3329/jsr.v2i1.2643                   J. Sci. Res. 2 (1), 144-157 (2010) 

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