scholarly journals Aspergillus nidulans: A Potential Resource of the Production of the Native and Heterologous Enzymes for Industrial Applications

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Amit Kumar
Keyword(s):  
Aspergillus Nidulans ◽  
Low Cost ◽  
Model Organism ◽  
Alcohol Oxidase ◽  
Industrial Applications ◽  
Genetically Engineered ◽  
Cell Factory ◽  
Industrial Enzymes ◽  
Fungal Cell ◽  
Potential Resource

Aspergillus nidulans is a filamentous fungus that is a potential resource for industrial enzymes. It is a versatile fungal cell factory that can synthesize various industrial enzymes such as cellulases, β-glucosidases, hemicellulases, laccases, lipases, proteases, β-galactosidases, tannases, keratinase, cutinases, and aryl alcohol oxidase. A. nidulans has shown the potential to utilize low-cost substrates such as wheat bran, rice straw, sugarcane bagasse, rice bran, coir pith, black gram residue, and chicken feathers to produce enzymes cost-effectively. A. nidulans has also been known as a model organism for the production of heterologous enzymes. Several studies reported genetically engineered strains of A. nidulans for the production of different enzymes. Native as well as heterologous enzymes of A. nidulans have been employed for various industrial processes.

scholarly journals Redesigning the Aspergillus nidulans xylanase regulatory pathway to enhance cellulase production with xylose as the carbon and inducer source

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Patrick Ballmann ◽  
Jorge Lightfoot ◽  
Michael Müller ◽  
Stephan Dröge ◽  
Rolf Prade
Keyword(s):  
Aspergillus Nidulans ◽  
Enzyme Production ◽  
Enzymatic Digestion ◽  
Cellulase Production ◽  
Genetically Engineered ◽  
Production Costs ◽  
Fungal Cell ◽  
Cell Factories ◽  
Genetic Interventions ◽  
Reducing Costs

Abstract Background Biomass contains cellulose (C6-sugars), hemicellulose (C5-sugars) and lignin. Biomass ranks amongst the most abundant hydrocarbon resources on earth. However, biomass is recalcitrant to enzymatic digestion by cellulases. Physicochemical pretreatment methods make cellulose accessible but partially destroy hemicellulose, producing a C5-sugar-rich liquor. Typically, digestion of pretreated LCB is performed with commercial cellulase preparations, but C5-sugars could in principle be used for “on site” production of cellulases by genetically engineered microorganism, thereby reducing costs. Results Here we report a succession of genetic interventions in Aspergillus nidulans that redesign the natural regulatory circuitry of cellulase genes in such a way that recombinant strains use C5-sugar liquors (xylose) to grow a vegetative tissue and simultaneously accumulate large amounts of cellulases. Overexpression of XlnR showed that under xylose-induction conditions only xylanase C was produced. XlnR overexpression strains were constructed that use the xynCp promoter to drive the production of cellobiohydrolases, endoglucanases and β-glucosidase. All five cellulases accumulated at high levels when grown on xylose. Production of cellulases in the presence of pretreated-biomass C5-sugar liquors was investigated, and cellulases accumulated to much higher enzyme titers than those obtained for traditional fungal cell factories with cellulase-inducing substrates. Conclusions By replacing expensive substrates with a cheap by-product carbon source, the use of C5-sugar liquors directly derived from LCB pretreatment processes not only reduces enzyme production costs, but also lowers operational costs by eliminating the need for off-site enzyme production, purification, concentration, transport and dilution.

Prevention of melanin formation during aryl alcohol oxidase production under growth-limited conditions using an Aspergillus nidulans cell factory

2017 ◽  
Vol 243 ◽  
pp. 874-882 ◽  
Author(s):  
Oscar Pardo-Planas ◽  
Rolf A. Prade ◽  
Michael Müller ◽  
Hasan K. Atiyeh ◽  
Mark R. Wilkins
Keyword(s):  
Aspergillus Nidulans ◽  
Alcohol Oxidase ◽  
Cell Factory ◽  
Melanin Formation

scholarly journals Remote Reflectivity Sensor for Industrial Applications

Sensors ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 1301
Author(s):  
Federico Cavedo ◽  
Parisa Esmaili ◽  
Michele Norgia
Keyword(s):  
Low Cost ◽  
Cost Effective ◽  
Industrial Applications ◽  
Interference Immunity ◽  
Surface Reflection ◽  
Measurement Systems ◽  
Digital Detector ◽  
Reflected Light ◽  
Surface Optical ◽  
Diffused Light

A low-cost optical reflectivity sensor is proposed in this paper, able to detect the presence of objects or surface optical properties variations, at a distance of up to 20 m. A collimated laser beam is pulsed at 10 kHz, and a synchronous digital detector coherently measures the back-diffused light collected through a 1-inch biconvex lens. The sensor is a cost-effective solution for punctual measurement of the surface reflection at different distances. To enhance the interference immunity, an algorithm based on a double-side digital baseline restorer is proposed and implemented to accurately detect the amplitude of the reflected light. As results show, the sensor is robust against ambient light and shows a strong sensitivity on a wide reflection range. The capability of the proposed sensor was evaluated experimentally for object detection and recognition, in addition to dedicated measurement systems, like remote encoders or keyphasors, realized far from the object to be measured.

scholarly journals Deconstruction of Lignin: From Enzymes to Microorganisms

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2299
Author(s):  
Jéssica P. Silva ◽  
Alonso R. P. Ticona ◽  
Pedro R. V. Hamann ◽  
Betania F. Quirino ◽  
Eliane F. Noronha
Keyword(s):  
Microbial Communities ◽  
Metabolic Pathways ◽  
Low Cost ◽  
Direct Analysis ◽  
Industrial Applications ◽  
Natural Environments ◽  
Lignocellulosic Residues ◽  
Complementary Techniques ◽  
Plant Polymer ◽  
Powerful Strategy

Lignocellulosic residues are low-cost abundant feedstocks that can be used for industrial applications. However, their recalcitrance currently makes lignocellulose use limited. In natural environments, microbial communities can completely deconstruct lignocellulose by synergistic action of a set of enzymes and proteins. Microbial degradation of lignin by fungi, important lignin degraders in nature, has been intensively studied. More recently, bacteria have also been described as able to break down lignin, and to have a central role in recycling this plant polymer. Nevertheless, bacterial deconstruction of lignin has not been fully elucidated yet. Direct analysis of environmental samples using metagenomics, metatranscriptomics, and metaproteomics approaches is a powerful strategy to describe/discover enzymes, metabolic pathways, and microorganisms involved in lignin breakdown. Indeed, the use of these complementary techniques leads to a better understanding of the composition, function, and dynamics of microbial communities involved in lignin deconstruction. We focus on omics approaches and their contribution to the discovery of new enzymes and reactions that impact the development of lignin-based bioprocesses.

scholarly journals Enhanced catalytic ozonation of ibuprofen using a 3D structured catalyst with MnO2 nanosheets on carbon microfibers

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Guhankumar Ponnusamy ◽  
Hajar Farzaneh ◽  
Yongfeng Tong ◽  
Jenny Lawler ◽  
Zhaoyang Liu ◽  
...  
Keyword(s):  
Removal Efficiency ◽  
Active Sites ◽  
Low Cost ◽  
Three Dimensional ◽  
Industrial Applications ◽  
Catalytic Ozonation ◽  
Heterogeneous Catalytic ◽  
Structured Catalyst ◽  
Dimensional Network ◽  
Carbon Microfibers

AbstractHeterogeneous catalytic ozonation is an effective approach to degrade refractory organic pollutants in water. However, ozonation catalysts with combined merits of high activity, good reusability and low cost for practical industrial applications are still rare. This study aims to develop an efficient, stable and economic ozonation catalyst for the degradation of Ibuprofen, a pharmaceutical compound frequently detected as a refractory pollutant in treated wastewaters. The novel three-dimensional network-structured catalyst, comprising of δ-MnO2 nanosheets grown on woven carbon microfibers (MnO2 nanosheets/carbon microfiber), was synthesized via a facile hydrothermal approach. Catalytic ozonation performance of Ibuprofen removal in water using the new catalyst proves a significant enhancement, where Ibuprofen removal efficiency of close to 90% was achieved with a catalyst loading of 1% (w/v). In contrast, conventional ozonation was only able to achieve 65% removal efficiency under the same operating condition. The enhanced performance with the new catalyst could be attributed to its significantly increased available surface active sites and improved mass transfer of reaction media, as a result of the special surface and structure properties of this new three-dimensional network-structured catalyst. Moreover, the new catalyst displays excellent stability and reusability for ibuprofen degradation over successive reaction cycles. The facile synthesis method and low-cost materials render the new catalyst high potential for industrial scaling up. With the combined advantages of high efficiency, high stability, and low cost, this study sheds new light for industrial applications of ozonation catalysts.

scholarly journals Redox initiation in semicontinuous polymerization to search for specific mechanical properties of copolymers

e-Polymers ◽  
2020 ◽  
Vol 20 (1) ◽  
pp. 613-623
Author(s):  
José Manuel Sandoval-Díaz ◽  
Francisco Javier Rivera-Gálvez ◽  
Marta Fernández-García ◽  
Carlos Federico Jasso-Gastinel
Keyword(s):  
Low Cost ◽  
Mechanical Analysis ◽  
Industrial Applications ◽  
Size Exclusion ◽  
Proton Nuclear Magnetic Resonance ◽  
Polymerization Reaction ◽  
Redox Initiation ◽  
Initiation System ◽  
Feeding Profile ◽  
Chain Composition

AbstractIn this work, for a semicontinuous emulsion polymerization reaction, it is shown that using a redox initiation system at 40°C, substantial modifications in copolymer chain composition with conversion can be easily obtained. To test controllable trajectories for comonomer feeding, linear and parabolic profiles were chosen to get different types of chain composition variations for the 50/50 w/w styrene/n-butyl acrylate system. For the “forced composition copolymers,” the molecular weight averages and distribution were obtained by size exclusion chromatography. The composition along conversion was followed by proton nuclear magnetic resonance to determine the weight composition distribution (WCD) of the copolymer chains. Mechanodynamic (dynamic-mechanical analysis), tensile, and hardness tests exhibited consistent results depending on the WCD that outcomes from the respective feeding profile. The results confirm that this methodology is of great potential for industrial applications when looking for synergy in copolymer properties, and low-cost processes.

A Mechatronic Approach to Compact Fluid Disc Valve Design

1995 ◽  
Vol 209 (2) ◽  
pp. 115-125
Author(s):  
G A Parker ◽  
Y B Sun
Keyword(s):  
Low Cost ◽  
Dynamic Performance ◽  
Operating Mode ◽  
Industrial Applications ◽  
Test Results ◽  
Valve Design ◽  
Electromagnetic Characteristics ◽  
High Control ◽  
Fail Safe ◽  
Pilot Valve

The work presented in this paper deals mainly with a mechatronic approach to compact disc valve design and concentrates on improvements to the disc valve electromagnetic characteristics, the diaphragm design and the dynamic performance. A novel diaphragm-disc force motor has been successfully developed incorporating a pair of permanent ring magnets. It has the advantages of low electric power consumption at the null position, dual-lane electrical structure for fail-safe operation, high control accuracy and should be competitive with existing torque motors due to its low cost and simple construction. The research involved designing and testing a prototype disc pilot valve with a dual-lane operating mode. The test results showed that the valve has satisfactory static and dynamic characteristics for industrial applications.

scholarly journals Mask R-CNN Based C. Elegans Detection with a DIY Microscope

Biosensors ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 257
Author(s):  
Sebastian Fudickar ◽  
Eike Jannik Nustede ◽  
Eike Dreyer ◽  
Julia Bornhorst
Keyword(s):  
Cell Biology ◽  
High Throughput Screening ◽  
Low Cost ◽  
Image Acquisition ◽  
Rapid Development ◽  
Model Organism ◽  
Large Data ◽  
Data Set ◽  
C Elegans ◽  
Do It Yourself

Caenorhabditis elegans (C. elegans) is an important model organism for studying molecular genetics, developmental biology, neuroscience, and cell biology. Advantages of the model organism include its rapid development and aging, easy cultivation, and genetic tractability. C. elegans has been proven to be a well-suited model to study toxicity with identified toxic compounds closely matching those observed in mammals. For phenotypic screening, especially the worm number and the locomotion are of central importance. Traditional methods such as human counting or analyzing high-resolution microscope images are time-consuming and rather low throughput. The article explores the feasibility of low-cost, low-resolution do-it-yourself microscopes for image acquisition and automated evaluation by deep learning methods to reduce cost and allow high-throughput screening strategies. An image acquisition system is proposed within these constraints and used to create a large data-set of whole Petri dishes containing C. elegans. By utilizing the object detection framework Mask R-CNN, the nematodes are located, classified, and their contours predicted. The system has a precision of 0.96 and a recall of 0.956, resulting in an F1-Score of 0.958. Considering only correctly located C. elegans with an [email protected] IoU, the system achieved an average precision of 0.902 and a corresponding F1 Score of 0.906.

Synthesis, Characterization, Applications, and Toxicity of Green Synthesized Nanoparticles

2021 ◽  
Vol 22 ◽  
Author(s):  
João Marcos Pereira Galúcio ◽  
Sorrel Godinho Barbosa de Souza ◽  
Arthur Abinader Vasconcelos ◽  
Alan Kelbis Oliveira Lima ◽  
Kauê Santana da Costa ◽  
...  
Keyword(s):  
Green Synthesis ◽  
Low Cost ◽  
Chemical Properties ◽  
Analytical Techniques ◽  
Industrial Applications ◽  
Synthesis Methods ◽  
Methods Of Synthesis ◽  
Food Applications ◽  
Living Organisms ◽  
Harmful Effects

: Nanotechnology is a cutting-edge area with numerous industrial applications. Nanoparticles are structures that have dimensions ranging from 1–100 nm which exhibit significantly different mechanical, optical, electrical, and chemical properties when compared with their larger counterparts. Synthetic routes that use natural sources, such as plant extracts, honey, and microorganisms are environmentally friendly and low-cost methods that can be used to obtain nanoparticles. These methods of synthesis generate products that are more stable and less toxic than those obtained using conventional methods. Nanoparticles formed by titanium dioxide, zinc oxide, silver, gold, and copper, as well as cellulose nanocrystals are among the nanostructures obtained by green synthesis that have shown interesting applications in several technological industries. Several analytical techniques have also been used to analyze the size, morphology, hydrodynamics, diameter, and chemical functional groups involved in the stabilization of the nanoparticles as well as to quantify and evaluate their formation. Despite their pharmaceutical, biotechnological, cosmetic, and food applications, studies have detected their harmful effects on human health and the environment; and thus, caution must be taken in uses involving living organisms. The present review aims to present an overview of the applications, the structural properties, and the green synthesis methods that are used to obtain nanoparticles, and special attention is given to those obtained from metal ions. The review also presents the analytical methods used to analyze, quantify, and characterize these nanostructures.

Measuring Optical Properties of Advanced Nano/Micro-Periodic Structures Fabricated by Multi-Exposure Interference Lithography

2020 ◽  
Author(s):  
Shuzo Masui ◽  
Masaki Michihata ◽  
Kiyoshi Takamasu ◽  
Satoru Takahashi
Keyword(s):  
Periodic Structures ◽  
Low Cost ◽  
Industrial Applications ◽  
Interference Lithography ◽  
High Dispersion ◽  
Optical Elements ◽  
Precise Control ◽  
Periodic Grating ◽  
Interference Fringes ◽  
Multiple Interference

Abstract Functional optical elements based on nano/micro-periodic structures have attracted much attention. Since the fabrication of these dual-periodic structures requires precise control of periodicity, the semiconductor process such as an electron beam lithography has been mainly employed. However, these techniques have problems with expensive and low throughput for industrial applications. Therefore, there remains a need for low cost and high throughput fabrication methods of dual-periodic structures. Then we developed a multi-exposure interference lithography (MEIL) system using rotational Lloyd’s mirror interferometer to overcome these problems. The advantages of interference lithography are a large processing area and low cost. Our developed rotational Lloyd’s mirror setup enables us to a highly precise superposition of multiple interference fringes by multi-exposure. Furthermore, we developed a measurement setup for reflective diffractive elements using a two axial rotating stage and measured the diffraction properties of the fabricated dual-periodic diffraction gratings. In this paper, as a demonstration, we succeeded in the fabrication of high-dispersion diffraction grating with an enhanced diffraction efficiency of the −3rd order light. The fabricated shapes have a periodicity of 1997 nm and 665 nm. Furthermore, it was confirmed that the intensity of the −3rd order light was enhanced by about 10 times compared to the single periodic grating.

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