scholarly journals Toxoplasmosis Presenting as Nonhealing Cutaneous Ulcer

2020 ◽  
Vol 2020 ◽  
pp. 1-3
Author(s):  
M. Adhikari ◽  
S. Dhakal ◽  
S. Bhattarai ◽  
U. Rai
Keyword(s):  
Inflammatory Cell ◽  
Plasma Cells ◽  
Periodic Acid ◽  
Giant Cells ◽  
Incisional Biopsy ◽  
Periodic Acid Schiff ◽  
Left Hand ◽  
Cutaneous Ulcer ◽  
One Year ◽  
Immunocompetent Patients

Introduction. Systemic manifestation of toxoplasmosis is commonly seen in immune-compromised individuals. Skin manifestations are seen commonly in conjunction with systemic features. Isolated cutaneous toxoplasmosis is extraordinarily rare in immunocompetent patients. Case Description. A 64-year-old female presented to the Dermatology Outpatient Department (OPD), with a nonhealing ulcer over dorsum of the left hand for one year. The patient did not have any systemic diseases. Serology tests were negative. An incisional biopsy of the lesion revealed dense inflammatory cell infiltrates comprising predominantly of plasma cells and lymphocytes, multinucleated giant cells, and focal abscess formation in the dermis. Periodic Acid Schiff (PAS) stain showed organisms in the dermis with morphological resemblance to tachyzoites of Toxoplasma gondii. Conclusion. Though rare, a possibility of primary cutaneous toxoplasmosis should always be considered and looked for, even in immunocompetent patients presenting with chronic nonhealing ulcers.

scholarly journals  Histological and histochemical studies on the Harderian gland in native chickens

2012 ◽  
Vol 57 (No. 8) ◽  
pp. 404-409 ◽  
Author(s):  
B. Mobini
Keyword(s):  
Epithelial Cells ◽  
Plasma Cells ◽  
Periodic Acid ◽  
Harderian Gland ◽  
Histochemical Staining ◽  
Alveolar Type ◽  
Periodic Acid Schiff ◽  
Parasympathetic Ganglia ◽  
Tissue Sections ◽  
Native Chickens

  The objective of this investigation was to study the histological and histochemical structure of the Harderian gland in native chickens. Samples were obtained from 10 male and 10 female adult healthy native chickens. Tissue sections were stained with haematoxylin eosin, Verhoeff’s, Masson’s trichrome, alcian blue (pH 2.5), periodic acid-Schiff and Gomori’s method for reticulum. The multilobular Harderian gland of native chickens was covered by a thin connective tissue which consisted of adipose tissue, parasympathetic ganglia, nerve bundles, collagen, elastic and reticular fibres. Plasma cells were present in interlobular areas. The Harderian gland was compound tubulo-alveolar type. The Harderian duct was lined by columnar epithelial cells of varying height. Goblet cells were not found in Harderian duct. Histochemical staining revealed that the all epithelial cells of both corpus glandulae and ducts contained both neutral and acidic mucins. No significant sex-based differences were found. It is concluded that the general histological and histochemical structure of the Harderian gland in native chickens is similar to that of domestic geese, but that there are also some differences.  

Plasma Cell Leukemia: A Report on Three Patients

1983 ◽  
Vol 69 (6) ◽  
pp. 589-591 ◽  
Author(s):  
Leonardo Pacilli ◽  
Paolo Ferraro ◽  
Silvia Cochi ◽  
Antonio De Laurenzi
Keyword(s):  
Plasma Cell ◽  
Peripheral Blood ◽  
Plasma Cells ◽  
Periodic Acid ◽  
Previous History ◽  
Bone Marrow Aspiration ◽  
Plasma Cell Leukemia ◽  
Cell Leukemia ◽  
Periodic Acid Schiff ◽  
The Third

Three patients with plasma cell leukemia are reported. Two of them had a previous history of myeloma; the third one started with a plasma cell leukemia. Diagnosis was made from the required presence of 20% plasma cells in the peripheral blood. In all 3 cases, bone marrow aspiration and peripheral blood showed plasma cells strongly positive for acid phosphatase and alpha-naphthyl acetate esterase, and negative for periodic acid-Schiff. The first patient was treated with a polychemotherapy regimen that included vincristine, cyclophosphamide, chlorambucil and prednisone, and the second patient with melphalan and prednisone; the third one, who started with plasma cell leukemia, received total body irradiation at the dose of 600 rad. The results of the therapy and survival time, which was never more than 3 months, are in accord with other reports in the literature.

scholarly journals Developmental Changes of Lymphoid Tissue in the Harderian Gland of Laying Hens

2014 ◽  
Vol 37 (1) ◽  
pp. 83-88 ◽  
Author(s):  
Pamela Bejdić ◽  
Rizah Avdić ◽  
Ljiljana Amidžić ◽  
Velida Ćutahija ◽  
Faruk Tandir ◽  
...  
Keyword(s):  
Lymphoid Tissue ◽  
Plasma Cells ◽  
Laying Hens ◽  
Periodic Acid ◽  
Harderian Gland ◽  
Cell Types ◽  
Methyl Green ◽  
Periodic Acid Schiff ◽  
Tissue Sections ◽  
Lymphoid Nodules

Abstract The Harderian gland of 110 laying hens was histologically investigated from the time of hatching to the period of 10 months of age. Tissue sections were stained with haematoxylin and eosin, periodic acid-schiff (PAS) and methyl green-pyronin technique. The research shows that lymphoid tissue is colonised by three types of cells: heterophils, lymphocytes and plasma cells. The number of these cells is directly dependent on the bird’s age. During the lifetime of the hens there gradually comes a shift in the dominance of these three cell types. Lymphoid nodules are detected only in 40-day-old chickens, while later in adult birds the Harderian gland is the organ which contains the largest number of mature plasma cells. Some plasma cells contain Russell bodies with different size and shape.

scholarly journals Drug-related talaromycosis: A case report

2020 ◽  
Vol 34 ◽  
pp. 205873842093461
Author(s):  
Yongsheng Lu ◽  
Quan Shi ◽  
Jing Yu
Keyword(s):  
Drug Abuse ◽  
Periodic Acid ◽  
Fungal Spores ◽  
Public Health Problem ◽  
Giant Cells ◽  
Pathological Examination ◽  
Human Immune System ◽  
Periodic Acid Schiff ◽  
History Of ◽  
Anti Fungal

Talaromycosis is a rare deep fungal infection caused by Talaromyces marneffei. Currently, methamphetamine has become the second-largest drug abuse category in the world after cannabis and has become a serious public health problem. Methamphetamine can inhibit human immune system and increase the probability of pathogenic microorganism infection. On 8 October 2016, a 20-year-old man with a fever history of 2 months was admitted to our hospital. He had bloody stools and abdominal pain during hospitalization. There was no significant abnormality in physical examination. Because of the misdiagnosis, he underwent improper treatment. Periodic acid-Schiff stain (PAS) staining showed that the mucosa of distal ileum, ascending colon, transverse colon, and sigmoid colon were infiltrated by a large number of tissue cells, which contained a large number of blue purple particles. In addition, a large number of histiocytes and multinucleated giant cells can be seen in the lamina propria of ileum mucosa, and fungal spores can be seen in histiocytes. Finally, he was diagnosed as talaromycosis and took itraconazole 0.2 g twice a day. After 5 days, the temperature dropped to normal and the inflammation disappeared, and he continued to take itraconazole for 6 months. Due to the neglect of the history of drug abuse and the concealment, drug-related talaromycosis is often misdiagnosed. Pathological examination is warranted for diagnosis talaromycosis. This condition requires a long-term anti-fungal therapy.

Crystal-Storing Histiocytosis as a Cause of Symptomatic Cardiac Mass

2009 ◽  
Vol 133 (11) ◽  
pp. 1861-1864 ◽  
Author(s):  
Charles J. Sailey ◽  
Borislav A. Alexiev ◽  
James S. Gammie ◽  
Paula Pinell-Salles ◽  
J. Lawrence Stafford ◽  
...  
Keyword(s):  
Monoclonal Gammopathy ◽  
Plasma Cells ◽  
Periodic Acid ◽  
Right Atrial ◽  
Prior History ◽  
Low Grade ◽  
Periodic Acid Schiff ◽  
Right Atrial Mass ◽  
History Of

Abstract Crystal-storing histiocytosis is a rare disorder that is typically associated with low-grade B-cell lymphomas and monoclonal gammopathy. We present a 64-year-old man with a prior history of weakness and weight loss and hematologic evaluation that had revealed immunoglobulin G κ monoclonal light chains in the serum and negative bone marrow biopsy. He presented with supraventricular tachyarrhythmia and a right atrial mass seen on echocardiogram and excised surgically. Histologically, the tumor was composed of sheets of macrophages infiltrating the atrial myocardium. The histiocytes were filled with multiple needle-shaped, periodic acid-Schiff–negative crystals. These cells and associated plasma cells failed to show clonal light chain restriction by in situ hybridization or immunohistochemistry, and there was no area of lymphoma in the tumor. Ultrastructural examination showed numerous sticklike, trapezoidal, or polygonal dense crystals in the cytoplasm of histiocytes corroborating the diagnosis of crystal-storing histiocytosis. Although rare, crystal-storing histiocytosis should be included in the differential diagnosis of heart masses in patients with hematologic conditions associated with monoclonal gammopathy.

scholarly journals  A case of systemic mycosis in a Hovawart dog due to Candida albicans

2011 ◽  
Vol 56 (No. 5) ◽  
pp. 260-264 ◽  
Author(s):  
M. Skoric ◽  
P. Fictum ◽  
I. Slana ◽  
P. Kriz ◽  
I. Pavlik
Keyword(s):  
Candida Albicans ◽  
Histopathological Examination ◽  
Periodic Acid ◽  
Abdominal Cavity ◽  
Giant Cells ◽  
Health Condition ◽  
Periodic Acid Schiff ◽  
Tissue Samples ◽  
Generalized Lymphadenopathy ◽  
Systemic Infections

Candida albicans is reported as the etiological agent of multi-systemic infections in dogs. A two-year-old female Hovawart dog was presented with marked alteration in its health condition characterised by weakness, fever, anorexia, abdominal pain, cachexy and generalized lymphadenopathy. A radiograph of the abdominal cavity showed several non-specific nodular lesions in the mesentery, ranging in size up to 10 cm in diameter. At necropsy, extensive enlargement of lymph nodes and the presence of numerous whitish to grey nodules of different sizes in several organs were evident. Histopathological examination revealed pyogranulomatous inflammation characterized by large areas of necrosis surrounded by neutrophilic granulocytes, macrophages, multinucleated giant cells, and a variable admixture of lymphocytes and fungi-like organismsin in all affected organs. Numerous branching hyphae, subsequently identified by mycological cultivation as Candida albicans, were observed. A periodic acid Schiff (PAS) reaction to prove the presence of fungi in tissues was positive. Examination of tissue samples of affected organs using polymerase chain reaction (quantitative Real-Time PCR) and cultivation was negative for the presence of all members of the Mycobacterium tuberculosis complex, M. avium subsp. avium and M. avium subsp. hominissuis.

scholarly journals Peritoneal Sarcoidosis Mimicking Peritoneal Tuberculosis and Advanced Ovarian Carcinoma

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Brittany N. Robles ◽  
Catherine Shea ◽  
Ghadir Salame
Keyword(s):  
Local Community ◽  
Periodic Acid ◽  
Granulomatous Inflammation ◽  
Unknown Origin ◽  
Cyst Formation ◽  
Giant Cells ◽  
African American Female ◽  
Peritoneal Tuberculosis ◽  
Periodic Acid Schiff ◽  
Antituberculosis Treatment

Sarcoidosis is an inflammatory disease that affects one or multiple organs, most commonly the lungs and lymph nodes. This disease can present in a variety of ways which often makes diagnosis difficult. A 54-year-old postmenopausal African American female with a history of omental carcinomatosis of unknown origin was referred to the gynecology-oncology service at a local community hospital following a laparoscopic incarcerated hernia repair where multiple abdominal lesions suspicious of ovarian carcinomatosis were visualized. She was brought to the operating room for a diagnostic laparoscopy at which point the intra-abdominal survey revealed white tubercle-like lesions that were consistent with peritoneal tuberculosis. The lesions were excised and sent to pathology. The omentum biopsy was originally reported as adipose tissue showing focal fibrosis, focal mild acute inflammation, few cyst formation, and multiple granulomatous chronic inflammation, with multinucleated giant cells. Periodic acid-Schiff stain and acid fast bacilli stain were negative, and a diagnosis of peritoneal tuberculosis was made. The patient was started on an antituberculosis treatment regimen; however, she was not improving. The pathology slides were reexamined and revealed nonnecrotizing granulomatous inflammation consistent with sarcoidosis. The patient was immediately referred to the department of pulmonology and rheumatology, at which point she was started on corticosteroids and had an improvement in her condition.

Extranodal Lymphoplasmacytoid Lymphoma (Immunocytoma) Presenting as Small Intestinal Obstruction

2001 ◽  
Vol 125 (5) ◽  
pp. 677-679 ◽  
Author(s):  
Monica A. Recine ◽  
Maria T. Perez ◽  
Beria Cabello-Inchausti ◽  
Rogerio C. Lilenbaum ◽  
Morton J. Robinson
Keyword(s):  
Intestinal Obstruction ◽  
Unusual Case ◽  
Plasma Cells ◽  
Periodic Acid ◽  
Exploratory Laparotomy ◽  
Intestinal Wall ◽  
Immunoglobulin M ◽  
Periodic Acid Schiff ◽  
Systemic Involvement ◽  
Small Intestinal

Abstract A 57-year-old woman presented with intermittent symptoms of intestinal obstruction. Workup provided nondiagnostic radiologic studies. An exploratory laparotomy revealed a segmental dilatation in the proximal ileum, which showed diffuse thickening of the intestinal wall. Microscopic examination of the affected area disclosed a diffuse transmural infiltrate composed of small lymphocytes, mature plasma cells, and lymphoplasmacytoid cells in different stages of maturation associated with extracellular periodic acid-Schiff–positive material. In addition, serum protein electrophoresis showed a monoclonal immunoglobulin M κ paraprotein. Postoperative workup did not demonstrate evidence of systemic involvement. The morphologic features and immunohistochemical and molecular analyses were consistent with lymphoplasmacytoid lymphoma (immunocytoma). We report an unusual case of primary extranodal immunocytoma involving the small intestine and discuss its clinicopathologic features.

scholarly journals Histoplasmosis of the Head and Neck in the Immunocompetent Patient: Report of 2 Cases

2018 ◽  
Vol 97 (9) ◽  
pp. E28-E31 ◽  
Author(s):  
Ashley P. O'Connell Ferster ◽  
Aaron Jaworek ◽  
Amanda Hu
Keyword(s):  
Head And Neck ◽  
Treatment Options ◽  
Periodic Acid ◽  
Immunocompetent Patient ◽  
Accurate Diagnosis ◽  
Antibody Detection ◽  
Common Disease ◽  
Periodic Acid Schiff ◽  
Microscopic Appearance ◽  
Immunocompetent Patients

Histoplasmosis of the head and neck is rarely seen in immunocompetent patients. We report 2 new cases of histoplasmosis of the head and neck in immunocompetent patients, one an 80-year-old man and the other a 57-year-old man. The older man presented with oral cavity histoplasmosis; his symptoms included pain, dysphagia, and ulcerative lesions. The younger man had laryngeal histoplasmosis, which resulted in hoarseness and dyspnea. We discuss the methods of diagnosis and the classic findings in histoplasmosis, including the microscopic appearance of caseating granulomas, the results of periodic acid–Schiff staining and Gomori staining, and antibody detection of histoplasmosis. We also review the treatment options with antifungals, including amphotericin B and the oral conazole drugs. With an accurate diagnosis and proper treatment, both of our patients recovered well and their symptoms resolved. Because their symptoms overlapped with those of other, more common disease processes, an accurate diagnosis of these patients was essential to treating their infection.

scholarly journals Incidental Mycobacterium-induced granulomatous inflammation of the follicular pharyngeal tonsils in a South African farmed ostrich (Struthio camelus)

2013 ◽  
Vol 84 (1) ◽  
Author(s):  
Martina R. Crole ◽  
John T. Soley ◽  
Sarah J. Clift
Keyword(s):  
Periodic Acid ◽  
Granulomatous Inflammation ◽  
Giant Cells ◽  
Lymphoid Cells ◽  
Presumptive Diagnosis ◽  
Periodic Acid Schiff ◽  
Tissue Mass ◽  
Caseous Necrosis ◽  
Mycobacterium Spp ◽  
Apparently Healthy

Avian mycobacteriosis (AM) is a zoonotic disease caused by Mycobacterium aviumcomplex (MAC), which can spread from avians to other farmed animals such as cattle and pigs as well as to humans. This study is the first report of granulomatous inflammation, as a result of avian mycobacteriosis, in the follicular pharyngeal tonsils of a farmed ostrich. The head of an apparently healthy farmed adult ostrich was obtained after slaughter. Each pharyngeal fold displayed a large tissue mass. This tissue was routinely prepared for light microscopy and stained with haematoxylin and eosin, periodic acid Schiff, Grocott methenamine silver, Gram and Ziehl-Neelsen. Immunohistochemistry (IHC) and polymerase chain reaction (PCR) were performed to identify Mycobacterium spp. and Mycobacterium tuberculosis complex, respectively. Histologically, the tissue masses consisted of confluent mature micro-granulomata that were characterised by central caseous necrosis surrounded by multinucleated giant cells, macrophages and lymphoid cells and an outer mature fibrous connective tissue capsule. Within some foci of caseous necrosis were variably sized colonies of small, Gram-negative, acid-fast bacilli, which showed positive IHC labelling for Mycobacterium spp., leading to a presumptive diagnosis of AM. PCR thus proved useful in excluding the presence of notifiable Mycobacteriumspp. The significance and role of the pharyngeal tonsils of ratites in diseases such as AM warrant specific attention. Moreover, as ratites are known to present with AM infections with apparently no visible loss in body condition, as presumably occurred in the present case, it is imperative that unusual masses in apparently healthy ratites be thoroughly investigated.

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