scholarly journals Peritoneal Sarcoidosis Mimicking Peritoneal Tuberculosis and Advanced Ovarian Carcinoma

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Brittany N. Robles ◽  
Catherine Shea ◽  
Ghadir Salame
Keyword(s):  
Local Community ◽  
Periodic Acid ◽  
Granulomatous Inflammation ◽  
Unknown Origin ◽  
Cyst Formation ◽  
Giant Cells ◽  
African American Female ◽  
Peritoneal Tuberculosis ◽  
Periodic Acid Schiff ◽  
Antituberculosis Treatment

Sarcoidosis is an inflammatory disease that affects one or multiple organs, most commonly the lungs and lymph nodes. This disease can present in a variety of ways which often makes diagnosis difficult. A 54-year-old postmenopausal African American female with a history of omental carcinomatosis of unknown origin was referred to the gynecology-oncology service at a local community hospital following a laparoscopic incarcerated hernia repair where multiple abdominal lesions suspicious of ovarian carcinomatosis were visualized. She was brought to the operating room for a diagnostic laparoscopy at which point the intra-abdominal survey revealed white tubercle-like lesions that were consistent with peritoneal tuberculosis. The lesions were excised and sent to pathology. The omentum biopsy was originally reported as adipose tissue showing focal fibrosis, focal mild acute inflammation, few cyst formation, and multiple granulomatous chronic inflammation, with multinucleated giant cells. Periodic acid-Schiff stain and acid fast bacilli stain were negative, and a diagnosis of peritoneal tuberculosis was made. The patient was started on an antituberculosis treatment regimen; however, she was not improving. The pathology slides were reexamined and revealed nonnecrotizing granulomatous inflammation consistent with sarcoidosis. The patient was immediately referred to the department of pulmonology and rheumatology, at which point she was started on corticosteroids and had an improvement in her condition.

scholarly journals Incidental Mycobacterium-induced granulomatous inflammation of the follicular pharyngeal tonsils in a South African farmed ostrich (Struthio camelus)

2013 ◽  
Vol 84 (1) ◽  
Author(s):  
Martina R. Crole ◽  
John T. Soley ◽  
Sarah J. Clift
Keyword(s):  
Periodic Acid ◽  
Granulomatous Inflammation ◽  
Giant Cells ◽  
Lymphoid Cells ◽  
Presumptive Diagnosis ◽  
Periodic Acid Schiff ◽  
Tissue Mass ◽  
Caseous Necrosis ◽  
Mycobacterium Spp ◽  
Apparently Healthy

Avian mycobacteriosis (AM) is a zoonotic disease caused by Mycobacterium aviumcomplex (MAC), which can spread from avians to other farmed animals such as cattle and pigs as well as to humans. This study is the first report of granulomatous inflammation, as a result of avian mycobacteriosis, in the follicular pharyngeal tonsils of a farmed ostrich. The head of an apparently healthy farmed adult ostrich was obtained after slaughter. Each pharyngeal fold displayed a large tissue mass. This tissue was routinely prepared for light microscopy and stained with haematoxylin and eosin, periodic acid Schiff, Grocott methenamine silver, Gram and Ziehl-Neelsen. Immunohistochemistry (IHC) and polymerase chain reaction (PCR) were performed to identify Mycobacterium spp. and Mycobacterium tuberculosis complex, respectively. Histologically, the tissue masses consisted of confluent mature micro-granulomata that were characterised by central caseous necrosis surrounded by multinucleated giant cells, macrophages and lymphoid cells and an outer mature fibrous connective tissue capsule. Within some foci of caseous necrosis were variably sized colonies of small, Gram-negative, acid-fast bacilli, which showed positive IHC labelling for Mycobacterium spp., leading to a presumptive diagnosis of AM. PCR thus proved useful in excluding the presence of notifiable Mycobacteriumspp. The significance and role of the pharyngeal tonsils of ratites in diseases such as AM warrant specific attention. Moreover, as ratites are known to present with AM infections with apparently no visible loss in body condition, as presumably occurred in the present case, it is imperative that unusual masses in apparently healthy ratites be thoroughly investigated.

scholarly journals Pathology of cutaneous blastomycosis in a cat

2020 ◽  
Vol 4 (1) ◽  
pp. 3-4
Author(s):  
Vetnizah Juniantito ◽  
Eva Harlina ◽  
. Jumari ◽  
Vici Eko Handayani ◽  
Iis Ismawati
Keyword(s):  
Infectious Disease ◽  
Skin Biopsy ◽  
Periodic Acid ◽  
Granulomatous Inflammation ◽  
Giant Cells ◽  
Periodic Acid Schiff ◽  
Pet Owners ◽  
Granulomatous Dermatitis ◽  
Type Giant ◽  
Epidermal Necrosis

Cats are common as pet animals in Indonesia and live in close vicinity of human neighborhood, which make them potential for transmitting diseases to human. Blastomycosis is infectious disease caused by Blastomyces spp yeast. Here we describe morphopathology of blastomycosis found in skin of a Persian Cat. Grossly, the lesion were characterized by ulcerated wounds and multiple subcutaneous small nodules (2-5 mm in diameter) in the base of tail. Skin biopsy was made and further processed for histopathology.  Microscopically, the lesion consist of epidermal necrosis, dermatitis, with coalescing dermal granulomatous inflammation, characterized by epitheloid and foreign type giant cells infiltrates. Numerous spherical-shaped structures and pseudohyphae which are Periodic Acid Schiff (PAS)-positive consistent with yeast morphology were found within the granulomas; morphology is also consistent with Blastomyces spp yeasts. Conclusively, multiple granulomatous dermatitis with evidence of Blastomyces yeasts is a hallmark of cutaneous blastomycosis. Exposure to Blastomyces spp yeast may possess infection threat to pet owners.

scholarly journals Severe Disseminated Necrotizing and Granulomatous Lymphadenitis and Encephalitis in a Dog Due to Sporotrichum pruinosum (Teleomorph: Phanerochaete chrysosporium)

2017 ◽  
Vol 55 (2) ◽  
pp. 298-302 ◽  
Author(s):  
Drew R. Magstadt ◽  
Amanda J. Fales-Williams ◽  
Jean-Sébastien Palerme ◽  
Heather Flaherty ◽  
Tracy Lindquist ◽  
...  
Keyword(s):  
Phanerochaete Chrysosporium ◽  
Natural Infection ◽  
Periodic Acid ◽  
Granulomatous Inflammation ◽  
Acute Onset ◽  
Common Finding ◽  
Initial Examination ◽  
Periodic Acid Schiff ◽  
Fungal Organisms ◽  
Sporotrichum Pruinosum

A 9-year-old female mixed breed dog presented for an acute onset of anorexia, vomiting, and cough. Initial examination and diagnostics revealed a large multilobular cranial mediastinal mass with unidentified fungal organisms on cytology. The disease progressed in spite of therapy until the dog was euthanized 8 months later. Gross necropsy findings were a large multilobular intrathoracic mass, mild pleuritis, and generalized lymphadenopathy. Histologic evaluation showed granulomatous inflammation and necrosis with numerous 20- to 70-micron, periodic acid–Schiff- and Gomori methenamine silver-positive spherules effacing lymph node parenchyma, as well as severe inflammation within the midbrain. Endosporulation was a common finding, and large numbers of fungal hyphae were also present in affected areas. Ribosomal RNA gene sequencing found 100% identity to published sequences of Phanerochaete chrysosporium, the teleomorph form of Sporotrichum pruinosum. This is the first published report of disease caused by natural infection with this basidiomycete organism in animals.

scholarly journals Drug-related talaromycosis: A case report

2020 ◽  
Vol 34 ◽  
pp. 205873842093461
Author(s):  
Yongsheng Lu ◽  
Quan Shi ◽  
Jing Yu
Keyword(s):  
Drug Abuse ◽  
Periodic Acid ◽  
Fungal Spores ◽  
Public Health Problem ◽  
Giant Cells ◽  
Pathological Examination ◽  
Human Immune System ◽  
Periodic Acid Schiff ◽  
History Of ◽  
Anti Fungal

Talaromycosis is a rare deep fungal infection caused by Talaromyces marneffei. Currently, methamphetamine has become the second-largest drug abuse category in the world after cannabis and has become a serious public health problem. Methamphetamine can inhibit human immune system and increase the probability of pathogenic microorganism infection. On 8 October 2016, a 20-year-old man with a fever history of 2 months was admitted to our hospital. He had bloody stools and abdominal pain during hospitalization. There was no significant abnormality in physical examination. Because of the misdiagnosis, he underwent improper treatment. Periodic acid-Schiff stain (PAS) staining showed that the mucosa of distal ileum, ascending colon, transverse colon, and sigmoid colon were infiltrated by a large number of tissue cells, which contained a large number of blue purple particles. In addition, a large number of histiocytes and multinucleated giant cells can be seen in the lamina propria of ileum mucosa, and fungal spores can be seen in histiocytes. Finally, he was diagnosed as talaromycosis and took itraconazole 0.2 g twice a day. After 5 days, the temperature dropped to normal and the inflammation disappeared, and he continued to take itraconazole for 6 months. Due to the neglect of the history of drug abuse and the concealment, drug-related talaromycosis is often misdiagnosed. Pathological examination is warranted for diagnosis talaromycosis. This condition requires a long-term anti-fungal therapy.

scholarly journals  A case of systemic mycosis in a Hovawart dog due to Candida albicans

2011 ◽  
Vol 56 (No. 5) ◽  
pp. 260-264 ◽  
Author(s):  
M. Skoric ◽  
P. Fictum ◽  
I. Slana ◽  
P. Kriz ◽  
I. Pavlik
Keyword(s):  
Candida Albicans ◽  
Histopathological Examination ◽  
Periodic Acid ◽  
Abdominal Cavity ◽  
Giant Cells ◽  
Health Condition ◽  
Periodic Acid Schiff ◽  
Tissue Samples ◽  
Generalized Lymphadenopathy ◽  
Systemic Infections

Candida albicans is reported as the etiological agent of multi-systemic infections in dogs. A two-year-old female Hovawart dog was presented with marked alteration in its health condition characterised by weakness, fever, anorexia, abdominal pain, cachexy and generalized lymphadenopathy. A radiograph of the abdominal cavity showed several non-specific nodular lesions in the mesentery, ranging in size up to 10 cm in diameter. At necropsy, extensive enlargement of lymph nodes and the presence of numerous whitish to grey nodules of different sizes in several organs were evident. Histopathological examination revealed pyogranulomatous inflammation characterized by large areas of necrosis surrounded by neutrophilic granulocytes, macrophages, multinucleated giant cells, and a variable admixture of lymphocytes and fungi-like organismsin in all affected organs. Numerous branching hyphae, subsequently identified by mycological cultivation as Candida albicans, were observed. A periodic acid Schiff (PAS) reaction to prove the presence of fungi in tissues was positive. Examination of tissue samples of affected organs using polymerase chain reaction (quantitative Real-Time PCR) and cultivation was negative for the presence of all members of the Mycobacterium tuberculosis complex, M. avium subsp. avium and M. avium subsp. hominissuis.

A 62-Year-Old African American Woman With Stable Lymphadenopathy Over 5 Years

2019 ◽  
Vol 152 (Supplement_1) ◽  
pp. S110-S110
Author(s):  
Hanadi El Achi ◽  
William Glass ◽  
Miriam Velazquez ◽  
Zhihong Hu
Keyword(s):  
African American ◽  
Plasma Cells ◽  
African American Woman ◽  
American Woman ◽  
Granulomatous Inflammation ◽  
Chronic Fatigue ◽  
Giant Cells ◽  
Castleman Disease ◽  
African American Female ◽  
Mantle Zone

Abstract The patient is a 62-year-old African American female with medical history of Plummer Vinson syndrome and CT scan in 2014 showing lymphadenopathy of the porta hepatis. Needle core biopsy of enlarged lymph nodes revealed sheets of polytypic plasma cells with no aberrant B or T cells or granulomas or hyaline-like depositions identified. The findings were suspicious for unicentric Castleman disease. In 2019, the patient presented with chronic fatigue without other systemic symptoms. Contrast CT revealed persistent retroperitoneal lymphadenopathy and calcified granulomas in the liver, spleen, and lung. Excision of portal and celiac nodes showed preserved nodal architecture with mild mantle zone expansion and marked hyaline-like deposition in the follicles. Sheets of mature plasma cells and focally increased vasculatures were seen in the interfollicular areas. Focally frequent nonnecrotizing granulomas were noted. Occasional giant cells and rare asteroid bodies were identified in the granulomas. Immunohistochemistry stain workup revealed normal distribution of the B and T lymphocytes. CD138 stain highlighted marked increased plasma cells and HHV8 was negative. Immunofluorescence microscopy for kappa and lambda did not show monoclonality; IgA was positive in <10% of plasma cells; special stain for Congo red and serum amyloid associated protein (SSA) IHC stain were strongly positive in the follicles. The overall findings are consistent with reactive lymphadenopathy with interfollicular polytypic plasmacytosis, noncaseating granuloma formation, and reactive amyloidosis. The features of mantle zone hyperplasia and polytypic plasmacytosis are suggestive of HHV8-negative/idiopathic unicentric Castleman disease. This case possibly represents an example of concurrent amyloidosis, sarcoidosis, and idiopathic unicentric Castleman disease. Given that SSA was recently reported to have a pathogenic role in granulomatous inflammation of sarcoidosis through sustained inflammatory reaction, the possibility of increased SSA triggering sarcoidoisis in this patient can be considered. However, the exact pathogenesis of these concurrent findings needs further investigation and clarification.

Malakoplakia of the Bladder in a 4-Month-Old Puppy

2019 ◽  
Vol 55 (5) ◽  
pp. 261-265
Author(s):  
Caroline Benzimra ◽  
Chloé Job ◽  
Quentin Pascal ◽  
Stéphane Bureau ◽  
Anaïs Combes ◽  
...  
Keyword(s):  
Histological Examination ◽  
Periodic Acid ◽  
Granulomatous Inflammation ◽  
Abdominal Ultrasound ◽  
Wall Thickening ◽  
Periodic Acid Schiff ◽  
E Coli ◽  
Mass Lesions ◽  
Lost To Follow Up

ABSTRACT A 4 mo old female Staffordshire bull terrier puppy was presented with chronic Escherichia coli cystitis. Ultrasound and cystoscopic examination revealed innumerable, intraluminal, finger-like proliferations arising from the dorsal urinary bladder (UB) wall. Histological examination of mucosal biopsies obtained by cystoscopy was suggestive of granulomatous cystitis. The proliferative lesions were removed surgically and submitted for histological examination. The UB submucosa was heavily infiltrated by macrophages with periodic acid-Schiff–positive cytoplasm exhibiting rare Michaelis-Gutmann bodies, leading to the diagnosis of malakoplakia. The puppy was prescribed with sulfamethoxazole-trimethoprim. The urinary signs disappeared despite the persistent UB wall thickening revealed by abdominal ultrasound. Urine culture performed during the ninth week of treatment showed a persistent infection by E coli resistant to sulfamethoxazole-trimethoprim. The dog was switched to doxycycline but was then lost to follow-up. Malakoplakia is a chronic granulomatous inflammation well documented in humans. Its pathophysiology is not fully understood, but bacterial infection, immunodepression, and a defective lysosomal function may lead to the intracytoplasmic accumulation of partially degraded bacteria that can subsequently mineralize to form the Michaelis-Gutmann bodies. Malakoplakia should be suspected when UB mass lesions are identified in a young dog with bacterial cystitis.

scholarly journals Periodic Acid–Schiff Positive Giant Cells in the Mouse Thymus Cortex

Nature ◽  
1961 ◽  
Vol 191 (4785) ◽  
pp. 305-305 ◽  
Author(s):  
D. METCALF ◽  
M. ISHIDATE
Keyword(s):  
Periodic Acid ◽  
Giant Cells ◽  
Periodic Acid Schiff ◽  
Mouse Thymus

scholarly journals Toxoplasmosis Presenting as Nonhealing Cutaneous Ulcer

2020 ◽  
Vol 2020 ◽  
pp. 1-3
Author(s):  
M. Adhikari ◽  
S. Dhakal ◽  
S. Bhattarai ◽  
U. Rai
Keyword(s):  
Inflammatory Cell ◽  
Plasma Cells ◽  
Periodic Acid ◽  
Giant Cells ◽  
Incisional Biopsy ◽  
Periodic Acid Schiff ◽  
Left Hand ◽  
Cutaneous Ulcer ◽  
One Year ◽  
Immunocompetent Patients

Introduction. Systemic manifestation of toxoplasmosis is commonly seen in immune-compromised individuals. Skin manifestations are seen commonly in conjunction with systemic features. Isolated cutaneous toxoplasmosis is extraordinarily rare in immunocompetent patients. Case Description. A 64-year-old female presented to the Dermatology Outpatient Department (OPD), with a nonhealing ulcer over dorsum of the left hand for one year. The patient did not have any systemic diseases. Serology tests were negative. An incisional biopsy of the lesion revealed dense inflammatory cell infiltrates comprising predominantly of plasma cells and lymphocytes, multinucleated giant cells, and focal abscess formation in the dermis. Periodic Acid Schiff (PAS) stain showed organisms in the dermis with morphological resemblance to tachyzoites of Toxoplasma gondii. Conclusion. Though rare, a possibility of primary cutaneous toxoplasmosis should always be considered and looked for, even in immunocompetent patients presenting with chronic nonhealing ulcers.

Conjunctival Candidiasis Mimicking Ocular Surface Squamous Neoplasia

2021 ◽  
Author(s):  
Zhiyu Peng ◽  
Jiang Qian ◽  
Yinan Han
Keyword(s):  
Candida Albicans ◽  
Ocular Surface ◽  
Maxillary Sinusitis ◽  
Squamous Metaplasia ◽  
Periodic Acid ◽  
Granulomatous Inflammation ◽  
Excisional Biopsy ◽  
Ocular Surface Squamous Neoplasia ◽  
Silver Stain ◽  
Periodic Acid Schiff

Abstract PurposeTo report a case of conjunctival candidiasis mimicking ocular surface squamous neoplasia. Case presentationA 71-year-old man presented with a history of persistent redness, swelling and watering in the left eye accompanying an enlarging mass in the conjunctiva. He underwent excisional biopsy which showed granulomatous inflammation accompanied by irregular and atypical squamous epithelium hyperplasia. Periodic acid-Schiff stain and methenamine silver stain revealed a fungi infection. Further secretion smear was performed to clarify the pathogen as Candida albicans and a chronic fungal maxillary sinusitis was found through imaging test. Thus a diagnosis of conjunctival candidiasis was made.ConclusionsConjunctivitis caused by fungi is rare and a trigger such as agriculture trauma, immunocompromise state, contact history to fungal environment or contaminated water or infection of adjacent organs occurs in most cases. We report the case not only to share diagnostic and treatment experience, but also describe the unique histopathological manifestation leading to a speculation that chronic fungal or candida albicans infection might induce squamous metaplasia.

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