scholarly journals Corrigendum to “Development and Characterization of an Electroless Plated Silver/Cysteine Sensor Platform for the Electrochemical Determination of Aflatoxin B1”

2020 ◽  
Vol 2020 ◽  
pp. 1-2
Author(s):  
Alex Paul Wacoo ◽  
Mathew Ocheng ◽  
Deborah Wendiro ◽  
Peter California Vuzi ◽  
Joseph F. Hawumba
Keyword(s):  
Aflatoxin B1 ◽  
Electrochemical Determination ◽  
Sensor Platform

scholarly journals Development and Characterization of an Electroless Plated Silver/Cysteine Sensor Platform for the Electrochemical Determination of Aflatoxin B1

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Alex Paul Wacoo ◽  
Mathew Ocheng ◽  
Deborah Wendiro ◽  
Peter California Vuzi ◽  
Joseph F. Hawumba
Keyword(s):  
Cyclic Voltammetry ◽  
Horseradish Peroxidase ◽  
Electroless Deposition ◽  
Limit Of Detection ◽  
Electrochemical Determination ◽  
Sensor Platform ◽  
Ft Ir ◽  
Aflatoxin B

An electroless plated silver/cysteine sensor platform [Glass|silver|cysteine|aflatoxin B1|horseradish peroxidase] for the Electrochemical detection of aflatoxin B1was developed and characterized. This involved four major steps: (1) an electroless deposition of silver (plating) onto a glass slide, (2) immobilization of cysteine; (3) conjugation of aflatoxin B1to cysteine groups; and (4) blocking of free cysteine groups with horseradish peroxidase (HRP). The binding of cysteine to the silver was demonstrated by the disappearance of thiol (S-H) groups at 2500 cm−1using Fourier transmittance infrared spectra (FT-IR), while the subsequent steps in the assembly of sensor platform were monitored using both FT-IR and cyclic voltammetry, respectively. The sensor platform exhibited a broadened nonsymmetrical redox couple as indicated by cyclic voltammetry. The platform was further characterized for sensitivity and limit of detection. The indirect competitive immunoassay format, whereby free and immobilized aflatoxin B1on the sensor competed for the binding site of free anti-aflatoxin B1antibody, was used at various concentrations of aflatoxin B1. The sensor generated differential staircase voltammogram that was inversely proportional to the concentration of aflatoxin B1and aflatoxin B1in the range of 0.06–1.1 ng/mL with a detection limit of 0.08 ng/mL could be detected.

Sensitive electrochemical determination of rhodamine B based on cyclodextrin-functionalized nanogold/hollow carbon nanospheres

2015 ◽  
Vol 7 (12) ◽  
pp. 4965-4970 ◽  
Author(s):  
Yinhui Yi ◽  
Heng Sun ◽  
Gangbing Zhu ◽  
Zhen Zhang ◽  
Xiangyang Wu
Keyword(s):  
Electrochemical Sensor ◽  
Rhodamine B ◽  
Electrochemical Determination ◽  
Carbon Nanospheres ◽  
Sensor Platform ◽  
Hollow Carbon

A sensitive electrochemical sensor platform for rhodamine B was developed based on cyclodextrin-functionalized nanogold/hollow carbon nanospheres.

scholarly journals Electrochemical Determination of the “Furanic Index” in Honey

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4115
Author(s):  
Severyn Salis ◽  
Nadia Spano ◽  
Marco Ciulu ◽  
Ignazio Floris ◽  
Maria I. Pilo ◽  
...  
Keyword(s):  
High Performance ◽  
Saturated Calomel Electrode ◽  
Electrochemical Determination ◽  
Chemical Marker ◽  
Simultaneous Reduction ◽  
Furoic Acid ◽  
Furanic Compounds

5-(hydroxymethyl)furan-2-carbaldehyde, better known as hydroxymethylfurfural (HMF), is a well-known freshness parameter of honey: although mostly absent in fresh samples, its concentration tends to increase naturally with aging. However, high quantities of HMF are also found in fresh but adulterated samples or honey subjected to thermal or photochemical stresses. In addition, HMF deserves further consideration due to its potential toxic effects on human health. The processes at the origin of HMF formation in honey and in other foods, containing saccharides and proteins—mainly non-enzymatic browning reactions—can also produce other furanic compounds. Among others, 2-furaldehyde (2F) and 2-furoic acid (2FA) are the most abundant in honey, but also their isomers (i.e., 3-furaldehyde, 3F, and 3-furoic acid, 3FA) have been found in it, although in small quantities. A preliminary characterization of HMF, 2F, 2FA, 3F, and 3FA by cyclic voltammetry (CV) led to hypothesizing the possibility of a comprehensive quantitative determination of all these compounds using a simple and accurate square wave voltammetry (SWV) method. Therefore, a new parameter able to provide indications on quality of honey, named “Furanic Index” (FI), was proposed in this contribution, which is based on the simultaneous reduction of all analytes on an Hg electrode to ca. −1.50 V vs. Saturated Calomel Electrode (SCE). The proposed method, validated, and tested on 10 samples of honeys of different botanical origin and age, is fast and accurate, and, in the case of strawberry tree honey (Arbutus unedo), it highlighted the contribution to the FI of the homogentisic acid (HA), i.e., the chemical marker of the floral origin of this honey, which was quantitatively reduced in the working conditions. Excellent agreement between the SWV and Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) data was observed in all samples considered.

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