scholarly journals Antibacterial Activity and Metabolomics Profiling of Torch Ginger (Etlingera elatior Jack) Flower Oil Extracted Using Subcritical Carbon Dioxide (CO2)

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Aliaa Anzian ◽  
Belal J. Muhialdin ◽  
Nameer Khairullah Mohammed ◽  
Hana Kadum ◽  
Anis Asyila Marzlan ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Antibacterial Activity ◽  
Bioactive Compound ◽  
Practical Applications ◽  
Food Industries ◽  
E Coli ◽  
H Nmr ◽  
Nmr Techniques ◽  
Pharmaceutical Industries ◽  
Carbon Dioxide Co2

The aim of this study was to identify the bioactive compound and evaluate the antibacterial activity of torch ginger flower oil extracted using subcritical carbon dioxide. The antibacterial activity was evaluated in agar diffusion assay, while MIC and MBC were determined using the microdilution broth assay. The essential oil was subjected to metabolomics profiling using GC-MS and 1H-NMR techniques. The results demonstrated strong antibacterial activity towards Salmonella typhimurium, Staphylococcus aureus, and Escherichia coli. The MIC values were 0.0625, 0.25, and 0.25 mg/mL, and the MBC values were 0.25, 0.5, and 1 mg/mL towards S. typhimurium, S. aureus, and E. coli, respectively. A total of 33 compounds were identified using GC-MS including 15 compounds (45%) known for their antimicrobial activity. In addition, sixteen metabolites were identified using NMR analysis and 8 out of the sixteen metabolites (50%) have antibacterial activity. The extracted oil demonstrated broad range for antibacterial activity and has high potential for applications in pharmaceutical and food industries. Practical Applications. The oil extracted from the torch ginger flower was found very stable and has promising applications as antibacterial agent for food and pharmaceutical industries.

scholarly journals SOLVENT FREE SYNTHESIS OF CHALCONE AS ANTIBACTERIAL SUBSTANCE

2017 ◽  
Vol 13 (1) ◽  
pp. 95
Author(s):  
Susy Yunita Prabawati ◽  
Arifah Khusnuryani ◽  
Khamidinal Khamidinal
Keyword(s):  
Antibacterial Activity ◽  
Solvent Free ◽  
Hydration Reaction ◽  
Antibacterial Substance ◽  
E Coli ◽  
H Nmr ◽  
Activity Test ◽  
Grinding Technique ◽  
Solvent Free Synthesis

<p>The aims of this study was to synthesize a compound of 3-methoxy-4-hydroxychalcone through Claisen-Schmidt condensation with grinding technique (solvent-free). Vanillin, acetophenone and 60 % NaOH catalyst used in this synthesis. Characterization of products was done with a spectrophotometer FTIR and <sup>1</sup>H-NMR spectrometer. The  product was obtained as a orange solid which has a melting point at    58 - 59 ˚C. Identification of the product by IR spectrophotometer showed the absorption of  C=C in the wavenumber of 1496.76 cm<sup>-1</sup> which confirmed that the hydration reaction of chalcone had occurred. Analysis using <sup>1</sup>H-NMR spectrometer also showed the proton of the CH=CH appearing on chemical shift (δ) 7.99 ppm. The antibacterial activity test showed that the compounds of 3-methoxy-4- hydroxychalcone has a potential as an antibacterial against bacteria <em>E. coli</em> and <em>B. subtilis</em>.</p>

scholarly journals Antibacterial activity of Escherichia coli from squid ink (Loligo sp.) n-Hexane extracts

2021 ◽  
Vol 869 (1) ◽  
pp. 012033
Author(s):  
D R Utami ◽  
I Irwan ◽  
S Agustina ◽  
S Karina ◽  
S Afriani
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Secondary Metabolite ◽  
Bioactive Compound ◽  
Inhibition Zone ◽  
Hexane Extract ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Hexane Extracts ◽  
Intermediate Concentration

Abstract Squid is one of the export commodities in Indonesia. In general, the use of squid meat, while the ink is only as waste. In fact, Squid ink contain bioactive compound that potential as anti-inflammatory, antihypertensive, anti-diabetic,anti-microbial and anti-malaria agents. The purpose of the study is to determine the types of secondary metabolite compounds contained in n-hexane extract of Loligo sp. ink using maceration method to determine its antibacterial activity against Escherichia coli. The results of secondary metabolite compounds obtained from the n-hexane extract of Loligo sp. ink are alkaloid, saponins, glycosides and phenol. The results of antibacterial test against E. coli using the disc method obtained the average of inhibition zone diameter at the concentration of 4% is 6.3 mm (intermediate), concentration of 8% is 7.83 mm (intermediate), concentration of 16% is 14.5 mm (susceptible) and concentration of 32% is 10.83 mm (intermediate). The antibacterial activity in n-hexane extract of Loligo sp. ink is optimal at the concentration of 16% against E. coli bacteria.

Reducing Pathogens by Using Zinc Oxide Nanoparticles and Acetic Acid in Sheep Meat

2014 ◽  
Vol 77 (9) ◽  
pp. 1599-1604 ◽  
Author(s):  
MAHBOUBEH MIRHOSSEINI ◽  
VAHID ARJMAND
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Acetic Acid ◽  
Zno Nanoparticles ◽  
Pathogenic Bacteria ◽  
Inhibitory Effect ◽  
Initial Growth ◽  
Practical Applications ◽  
E Coli ◽  
Sheep Meat

Practical applications of different concentrations (0, 1, 2, 4, 6, and 8 mM) of zinc oxide (ZnO) suspensions containing 1% acetic acid were investigated against the pathogenic bacteria Listeria monocytogenes, Escherichia coli, Staphylococcus aureus, and Bacillus cereus. ZnO suspensions (0, 1, 3, 6, and 8 mM) containing acetic acid had a significant inhibitory effect on the growth of L. monocytogenes, E. coli, and S. aureus during 12 h of incubation, and the 8 mM suspensions of ZnO were the most effective against all the strains. These data suggested that the antibacterial activity of ZnO was concentration dependent. Thus, 6 and 8 mM ZnO were selected for further studies in meat. ZnO nanoparticles reduced initial growth of all inoculated strains in meat. To our knowledge, this is the first report describing the antibacterial activity of ZnO nanoparticles in meat and indicates the potential of these nanoparticles as an antibacterial agent in the food industry.

scholarly journals Synthesis and Antibacterial Activity of Chalcones and Pyrimidine-2-ones

2005 ◽  
Vol 2 (2) ◽  
pp. 109-112
Author(s):  
A. K. Parekh ◽  
K. K. Desai
Keyword(s):  
Antibacterial Activity ◽  
Spectral Data ◽  
Elemental Analysis ◽  
Aromatic Aldehydes ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
H Nmr

Some new chalcones have been prepared by Claisen-schmidt condensation of ketone and different aromatic aldehydes. These chalcones on condensation with urea in presence of acid gave Pyrimidine-2-ones. The synthesized compounds have been characterized by elemental analysis, IR and1H NMR spectral data. They have been screened for their antibacterial activity against Gram positive bacteria B. subtillis & S. aureus and Gram negative bacteria E. coli & S. typhi.

scholarly journals Synthesis of Phthalyl Substituted Imidazolones and Schiff Bases as Antimicrobial Agents

2011 ◽  
Vol 8 (1) ◽  
pp. 427-434
Author(s):  
Pramilla Sah ◽  
Neha Saraswat ◽  
Manu Manu
Keyword(s):  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Schiff Bases ◽  
Phthalic Anhydride ◽  
Thionyl Chloride ◽  
Antimicrobial Agents ◽  
Spectral Techniques ◽  
E Coli ◽  
H Nmr ◽  
Ethanoic Acid

A new series of phthalyl substituted imidazolones (4a–g) and Schiff bases (5a–d) were synthesized from 2-methyl-(m-nitro-1,3-dioxo-1,3-dihydro-(2H)-isoindole-2-yl)-5-amino-1,3,4-thiadiazole (3a–b). Compounds (3a–b) were prepared by cyclisation of 2-(m-nitro-1,3-dioxo-1,3-dihydro-(2H)-isoindole-2-yl)methyl ethanoate (2) with thiosemicarbazide. 2-(m-nitro-1,3-dioxo-1,3-dihydro-(2H)-isoindole-2-yl)ethanoic acid (1) in presence of thionyl chloride and methanol gave the ester (2) while compound (1) was synthesized by aminolysis of phthalic anhydride with glycine. The compounds were characterized by spectral techniques of IR,1H NMR, Mass and elemental analysis. All the synthesized compounds (4a–g) and (5a–d) were screened for their antibacterial activity against the pathogenic strainsE. coli, P. aureus, C. freundiiwhile antifungal activity was evaluated againstA. niger, A. flavus, Penicillium sp. and C. albicans.

scholarly journals TRANSFORMATION OF ETHYL-P-METHOXYCINNAMATE TO P –METHOXYCINNAMIC ACID FROM KENCUR (Kaempheria galanga L.) AND THEIR ANTIBACTERIAL ACTIVITY

2017 ◽  
Vol 13 (2) ◽  
pp. 176
Author(s):  
Muhamad Salman Fareza ◽  
Rehana Rehana ◽  
Nuryanti Nuryanti ◽  
Didin Mujahidin
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Separation And Purification ◽  
E Coli ◽  
H Nmr ◽  
Alkaline Conditions ◽  
Methoxycinnamic Acid ◽  
Vacuum Liquid Chromatography ◽  
Hydrolysis Of ◽  
C Nmr

This study aimed to evaluate the antibacterial properties of ethyl-<em>p</em>-methoxycinnamate and <em>p</em>-methoxycinnamate acid from Kaempheria galanga L. Ethyl-<em>p</em>-methoxycinnamate was isolated from the <em>n</em>-hexane rhizome extract of <em>Kaempheria galanga L</em>. Separation and purification of this compound was carried out with vacuum liquid chromatography and column chromatography. Hydrolysis of ethyl-<em>p</em>-methoxycinnamic under alkaline conditions obtained <em>p</em>-methoxycinnamic acid with a good yield of 85 %. The structure of the compounds were charactrized with IR, NMR spectrophotometer (<sup>1</sup>H-NMR and <sup>13</sup>C-NMR) and mass spectrophotometer. The antibacterial properties of the compounds were evaluated using microdilution methods against <em>B. cereus</em> ATCC 11778, <em>L. monocytogenes</em> ATCC 7644, <em>E. coli</em> ATCC 25922, <em>S. enterica sv Typhimurium</em> ATCC 14028, and <em>E. aerogenes</em> ATCC 13048. The compounds showed weak antibacterial properties. Only ethyl <em>p</em>-methoxycinnamate showed the strongest antibacterial activity, especially against <em>B. cereus</em> ATCC 11778 bacteria with MIC values of 62.5 mg /mL. The change of the functional groups provided no significant impact on the antibacterial activity.

Synthesis of Silver Nanoparticle Using Bioactive Phenolic Compound Extracts of Leucas aspera and Leucas cephalotes and Evaluation of its Antibacterial Activity

2021 ◽  
Vol 6 (4) ◽  
pp. 310-314
Author(s):  
M. Salma Kausar ◽  
B.K. Manjunatha ◽  
C. Purvika ◽  
Mizba Farkana
Keyword(s):  
Antibacterial Activity ◽  
Silver Nanoparticle ◽  
Crude Extract ◽  
Bioactive Compound ◽  
Xrd Analysis ◽  
Klebsiella Pneumonia ◽  
Zone Of Inhibition ◽  
E Coli ◽  
Leucas Aspera ◽  
Phenolic Extract

The present study was carried out to investigate the antibacterial activity of the bioactive phenolic extract from Leucas aspera and Leucas cephalotes. The phenolic compounds were extracted using water: ethanol (1:3, v/v) by hydroethanolic extraction method. The hydroethanolic extracts were subjected to qualitative and FTIR analysis as a confirmatory step for the presence of phenolics. Synthesis of silver nanoparticle from both plants was carried out by acid hydrolysis method and subjected to UV-visible spectrophotometry, SEM, TEM and XRD analysis, for confirmation of tagged bioactive compound to AgNO3. The nanoparticle size distribution ranged between 50-94 nm in L. aspera and 40-67 nm in L. cephalotes. The antibacterial study was carried out using both crude phenolic extract and synthesized nanoparticles and tested against 5 pathogens namely Escherichia coli (ATCC® 8739™), Pseudomonas aeruginosa (ATCC® 25619™), Staphylococcus aureus (ATCC® 6538™), Bacillus subtilis (ATCC® 11774™) and Klebsiella pneumonia (ATCC® 13882™) for their antibacterial activity. From present study, the crude extract of L. cephalotes showed good antibacterial effect against test pathogen species wherein highest inhibition was observed in, P. aeruginosa, followed by B. subtilis and S. aureus with an average zone of inhibition of 23, 14 and 12 mm, E. coli and K. pneumonia measured 9 and 7 mm. The crude extract of L. aspera showed the highest inhibition in P. aeruginosa followed by S. aureus and E. coli with an average zone of inhibition of 12,11 and 10 mm B. subtilis and K. pneumonia measured 8 and 7 mm. Statistical analysis was calculated using One way ANOVA and was found to be statistically significant with p < 0.05.

scholarly journals Assembly of Carbon Dots into Frameworks with Enhanced Stability and Antibacterial Activity

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Pengfei Zhuang ◽  
Kuo Li ◽  
Daoyong Li ◽  
Haixia Qiao ◽  
Yifeng E ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Carbon Dots ◽  
Inhibitory Concentration ◽  
Hydrothermal Process ◽  
Active Surface ◽  
Practical Applications ◽  
E Coli ◽  
Antibacterial Performance ◽  
The Stability

AbstractCarbon dots (CDs) have been widely used as antimicrobials due to their active surface, but some CDs suffer instability. Therefore, the relative applications such as the antibacterial activity may not be reliable for long-term use. Herein, we synthesize CDs with blue fluorescence by a hydrothermal process. Thereafter, polyethylenimine was applied for the assembly of CDs into CDs-based frameworks (CDFs). The CDFs exhibited quenched fluorescence but showed more stable properties based on the scanning electron microscope and zeta potential investigations. Both CDs and CDFs show antibacterial activity toward Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus), but CDFs exhibited better antibacterial performance, and S. aureus could be completely inhibited with the minimum inhibitory concentration of 30 μg/mL. This reveals CDFs magnify both the stability and antibacterial activity, which would be more promising for practical applications. Graphic abstract

scholarly journals ANTIBACTERIAL ACTIVITY OF FOMITOPSIS BETULINA CULTURAL LIQUID

2019 ◽  
Vol 6 ◽  
pp. 10-16 ◽  
Author(s):  
Tetiana Krupodorova ◽  
Victor Barshteyn ◽  
Elena Pokas
Keyword(s):  
Antibacterial Activity ◽  
Antimicrobial Agents ◽  
Multidrug Resistant ◽  
Inhibitory Effect ◽  
Clinical Isolates ◽  
Bacterial Strains ◽  
Bacterial Diseases ◽  
E Coli ◽  
Pharmaceutical Industries ◽  
Fomitopsis Betulina

The antibacterial activity of Fomitopsis betulina cultural liquid (native, native concentrated, lyophilized, dried) against standard bacteria (Escherichia coli АТСС 25922, Pseudomonas aeruginosa АТСС 27853, Staphylococcus aureus АТСС 25923), and clinical isolates (Acinetobacter baumannii 50/1496 MBL, A. baumannii 88/2995 MBL, E. coli 116/3196 KPC, Klebsiella pneumoniae 6/509 ESBL, AmpC, KPC, P. aeruginosa 99/3066 MBL, P. aeruginosa 125/3343 MBL, S. haemoliticus 22/824 MRSA, S. aureus 134/3569 MRCNS) has been evaluated by the serial dilutions method. The antibacterial activity of F. betulina against S. haemoliticus and A. baumannii has been found for the first time. All samples of F. betulina cultural liquid demonstrated the inhibitory effect against standard bacterial strains at the minimum bactericidal concentration (MBC) ranging from >2.0 up to 18.75 mg/ml, and against multidrug-resistant clinical isolates with MBC from 7.8 up to 48.42 mg/ml. The dried F. betulina cultural liquid showed the highest antimicrobial activity against standard bacteria and clinical isolates, except A. baumannii 50/1496 MBL, while native concentrated cultural liquid was the most effective against this pathogen. The study showed that the antibacterial activity of the cultural liquid of F. betulina was improved by concentration and drying. The results obtained indicate that F. betulina cultural liquid contains alternative antimicrobial agents, useful for the treatment of bacterial diseases and might be a perspective substance for the pharmaceutical industries

scholarly journals Aktivitas Antibakteri Isolat Bakteri Endofit dari Tanaman Miana (Coleus scutellariodes [L.] Benth.) terhadap Staphylococcus aureus dan Escherichia coli

2014 ◽  
Vol 1 (1) ◽  
pp. 45-50
Author(s):  
Dwi Endah Kusumawati ◽  
Fachriyan Hasmi Pasaribu ◽  
Maria Bintang
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Endophytic Bacteria ◽  
Pathogenic Bacteria ◽  
Bioactive Compound ◽  
Antimicrobial Compounds ◽  
E Coli ◽  
Activity Test ◽  
The One ◽  
And Staphylococcus Aureus

Coleus scutellarioides [L.] Benth. known as medicinal plant which contains antidiarhea and antimicrobial compounds. Extraction of bioactive compound from plants is not efficient because it needs a large biomass, therefore the one of appropiate ways is using endophytic bacteria. The purpose of this research was to isolate and test of antibacterial activity of endophytic bacteria from Coleus scutellarioides [L.] Benth. against two pathogenic bacteria i.e Eschericia coli and Staphylococcus aureus. A total of 22 isolates of endophytic bacteria were obtained from Coleus scutellarioides [L.] Benth. Based on antibacterial activity test, 13 isolates of endophytic bacteria were able to inhibit the growth of E. coli and 15 isolates were able to inhibit S. aureus, whereas endophytic bacteria which can inhibit both types of pathogens are 10 isolates.

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