scholarly journals 3D Printing Decellularized Extracellular Matrix to Design Biomimetic Scaffolds for Skeletal Muscle Tissue Engineering

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Silvia Baiguera ◽  
Costantino Del Gaudio ◽  
Paolo Di Nardo ◽  
Vittorio Manzari ◽  
Felicia Carotenuto ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Extracellular Matrix ◽  
3D Printing ◽  
Muscle Regeneration ◽  
Large Scale ◽  
Structural Complexity ◽  
Patient Specific ◽  
Clinical Issue ◽  
Decellularized Extracellular Matrix ◽  
Patient Specific Implants

Functional engineered muscles are still a critical clinical issue to be addressed, although different strategies have been considered so far for the treatment of severe muscular injuries. Indeed, the regenerative capacity of skeletal muscle (SM) results inadequate for large-scale defects, and currently, SM reconstruction remains a complex and unsolved task. For this aim, tissue engineered muscles should provide a proper biomimetic extracellular matrix (ECM) alternative, characterized by an aligned/microtopographical structure and a myogenic microenvironment, in order to promote muscle regeneration. As a consequence, both materials and fabrication techniques play a key role to plan an effective therapeutic approach. Tissue-specific decellularized ECM (dECM) seems to be one of the most promising material to support muscle regeneration and repair. 3D printing technologies, on the other side, enable the fabrication of scaffolds with a fine and detailed microarchitecture and patient-specific implants with high structural complexity. To identify innovative biomimetic solutions to develop engineered muscular constructs for the treatment of SM loss, the more recent (last 5 years) reports focused on SM dECM-based scaffolds and 3D printing technologies for SM regeneration are herein reviewed. Possible design inputs for 3D printed SM dECM-based scaffolds for muscular regeneration are also suggested.

scholarly journals Sca-1 influences the innate immune response during skeletal muscle regeneration

2011 ◽  
Vol 300 (2) ◽  
pp. C287-C294 ◽  
Author(s):  
Kimberly K. Long ◽  
Grace K. Pavlath ◽  
Monty Montano
Keyword(s):  
Skeletal Muscle ◽  
Immune Response ◽  
Extracellular Matrix ◽  
Innate Immunity ◽  
Muscle Regeneration ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Primary Source ◽  
Innate Immune ◽  
Skeletal Muscle Regeneration

Efficient muscle regeneration requires the clearance of dead and dying tissue via phagocytosis before remodeling. We have previously shown that mice lacking stem cell antigen-1 (Sca-1) display a defect in skeletal muscle regeneration characterized by increased fibrosis and decreased turnover of the extracellular matrix. In the present study we demonstrate that Sca-1−/− mice have a defect in their capacity to recruit soluble IgM, and subsequently C3 complement, to damaged muscle. We hypothesize that this defect in recruitment delays or decreases phagocytosis by macrophages, contributing to the previously observed fibrotic phenotype of these mice. As the primary source of soluble IgM is peritoneal B-1a cells, which are a subset of self-renewing B cells, we analyzed this cell population and observed a significant reduction in B-1a cells in Sca-1−/− animals. Interestingly, these mice are protected from ischemia-reperfusion injury, an acute inflammatory reaction also mediated by IgM and C3 complement that has been linked to a deficit in B-1a cells in previous studies. Collectively, these data reveal a novel role for Sca-1 in innate immunity during muscle regeneration and indicate that further elucidation of immuno-myogenic processes will help to better understand and promote muscle regeneration.

3D Printing in Cardiovascular Disease: Current Applications and Future Perspectives

2021 ◽  
Author(s):  
Emanuele Verghi ◽  
Vincenzo Catanese ◽  
Antonio Nenna ◽  
Nunzio Mastroianni ◽  
Mario Lusini ◽  
...  
Keyword(s):  
Cardiovascular Disease ◽  
3D Printing ◽  
Perfect Match ◽  
Three Dimensional ◽  
Prosthetic Graft ◽  
Patient Specific ◽  
Future Perspectives ◽  
Patient Specific Implants ◽  
Life Saving ◽  
Traditional Pattern

Three-dimensional (3D) printing is emerging as an innovative tool for a tailored approach to endovascular or open procedures. The efforts of different specialists and data analysis can be used to fabricate patient-specific implants, which might have significant impact even in life-saving procedures such as aortic dissections or aortic arch aneurysm. 3D printing is gradually changing the traditional pattern of diagnosis and treatment. This innovative approach allows a perfect match between the patient's anatomy and the prosthetic graft, ideally resulting in better hemodynamics and improved long-term patency related to reduced turbulent flow. Future applications of 3D printing in the cardiovascular field combined with tissue engineering will enhance the therapeutic features of bioprinted tissues and scaffolds for regenerative medicine. This review will summarize the clinical significance of 3D printing in cardiovascular disease, exploring current applications, translational outlooks and future perspectives.

scholarly journals Recent Trends in Decellularized Extracellular Matrix Bioinks for 3D Printing: An Updated Review

2019 ◽  
Vol 20 (18) ◽  
pp. 4628 ◽  
Author(s):  
Kevin Dzobo ◽  
Keolebogile Shirley Caroline M. Motaung ◽  
Adetola Adesida
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
3D Printing ◽  
Regenerative Medicine ◽  
Treatment Options ◽  
3D Bioprinting ◽  
Characterization Methods ◽  
Physical Strength ◽  
Decellularized Extracellular Matrix ◽  
The Right

The promise of regenerative medicine and tissue engineering is founded on the ability to regenerate diseased or damaged tissues and organs into functional tissues and organs or the creation of new tissues and organs altogether. In theory, damaged and diseased tissues and organs can be regenerated or created using different configurations and combinations of extracellular matrix (ECM), cells, and inductive biomolecules. Regenerative medicine and tissue engineering can allow the improvement of patients’ quality of life through availing novel treatment options. The coupling of regenerative medicine and tissue engineering with 3D printing, big data, and computational algorithms is revolutionizing the treatment of patients in a huge way. 3D bioprinting allows the proper placement of cells and ECMs, allowing the recapitulation of native microenvironments of tissues and organs. 3D bioprinting utilizes different bioinks made up of different formulations of ECM/biomaterials, biomolecules, and even cells. The choice of the bioink used during 3D bioprinting is very important as properties such as printability, compatibility, and physical strength influence the final construct printed. The extracellular matrix (ECM) provides both physical and mechanical microenvironment needed by cells to survive and proliferate. Decellularized ECM bioink contains biochemical cues from the original native ECM and also the right proportions of ECM proteins. Different techniques and characterization methods are used to derive bioinks from several tissues and organs and to evaluate their quality. This review discusses the uses of decellularized ECM bioinks and argues that they represent the most biomimetic bioinks available. In addition, we briefly discuss some polymer-based bioinks utilized in 3D bioprinting.

Recent advances on 3D printing of patient-specific implants for fibrocartilage tissue regeneration

2018 ◽  
Vol 2 (3) ◽  
pp. 129-140 ◽  
Author(s):  
João B Costa ◽  
Joana Silva-Correia ◽  
Rui L Reis ◽  
Joaquim M Oliveira
Keyword(s):  
3D Printing ◽  
Tissue Regeneration ◽  
Patient Specific ◽  
Patient Specific Implants ◽  
Recent Advances

scholarly journals Tissue specific muscle extracellular matrix hydrogel improves skeletal muscle regeneration in vivo over non-matched tissue source

2020 ◽  
Author(s):  
Jessica L. Ungerleider ◽  
Monika Dzieciatkowska ◽  
Kirk C. Hansen ◽  
Karen L. Christman
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Extracellular Matrix ◽  
Regenerative Medicine ◽  
Expression Analysis ◽  
Muscle Regeneration ◽  
Gene Expression Analysis ◽  
Skeletal Muscle Regeneration ◽  
Cross Sectional ◽  
Tissue Specific

AbstractDecellularized extracellular matrix (ECM) hydrogels present a novel, clinical intervention for a myriad of regenerative medicine applications. The source of ECM is typically the same tissue to which the treatment is applied; however, the need for tissue specific ECM sources has not been rigorously studied. We hypothesized that tissue specific ECM would improve regeneration through preferentially stimulating physiologically relevant processes (e.g. progenitor cell proliferation and differentiation). One of two decellularized hydrogels (tissue specific skeletal muscle or non mesoderm-derived lung) or saline were injected intramuscularly two days after notexin injection in mice (n=7 per time point) and muscle was harvested at days 5 and 14 for histological and gene expression analysis. Both injectable hydrogels were decellularized using the same detergent and were controlled for donor characteristics (i.e. species, age). At day 5, the skeletal muscle ECM hydrogel significantly increased the density of Pax7+ satellite cells in the muscle. Gene expression analysis at day 5 showed that skeletal muscle ECM hydrogels increased expression of genes implicated in muscle contractility. By day 14, skeletal muscle ECM hydrogels improved muscle regeneration over saline and lung ECM hydrogels as shown through a shift in fiber cross sectional area distribution towards larger fibers. This data indicates a potential role for muscle-specific regenerative capacity of decellularized, injectable muscle hydrogels. Further transcriptomic analysis of whole muscle mRNA indicates the mechanism of tissue specific ECM-mediated tissue repair may be immune and metabolism pathway-driven. Taken together, this suggests there is benefit in using tissue specific ECM for regenerative medicine applications.

scholarly journals A Multi-Criteria Assessment Strategy for 3d Printed Porous Polyetheretherketone (Peek) Patient-Specific Implants for Orbital Wall Reconstruction

2021 ◽  
Author(s):  
Neha Sharma ◽  
Dennis Welker ◽  
Soheila Aghlmandi ◽  
Michaela Maintz ◽  
Hans-Florian Zeilhofer ◽  
...  
Keyword(s):  
3D Printing ◽  
Three Dimensional ◽  
Patient Specific ◽  
Fused Filament Fabrication ◽  
Decision Matrix ◽  
Maximum Deformation ◽  
Patient Specific Implants ◽  
High Durability ◽  
Orbital Wall ◽  
3D Printed

Pure orbital blowout fractures occur within the confines of the internal orbital wall. Restoration of orbital form and volume is paramount to prevent functional and esthetic impairment. The anatomical peculiarity of the orbit has encouraged surgeons to develop implants with customized features to restore its architecture. This has resulted in worldwide clinical demand for patient-specific implants (PSIs) designed to fit precisely in the patient's unique anatomy. Fused filament fabrication (FFF) three-dimensional (3D) printing technology has enabled the fabrication of implant-grade polymers such as Polyetheretherketone (PEEK), paving the way for a more sophisticated generation of biomaterials. This study evaluates the FFF 3D printed PEEK orbital mesh customized implants with a metric considering the relevant design, biomechanical, and morphological parameters. The performance of the implants is studied as a function of varying thicknesses and porous design constructs through a finite element (FE) based computational model and a decision matrix based statistical approach. The maximum stress values achieved in our results predict the high durability of the implants, and the maximum deformation values were under one-tenth of a millimeter (mm) domain in all the implant profile configurations. The circular patterned implant (0.9 mm) had the best performance score. The study demonstrates that compounding multi-design computational analysis with 3D printing can be beneficial for the optimal restoration of the orbital floor.

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