Bioinspired electrospun decellularized extracellular matrix scaffolds promote muscle regeneration in a rat skeletal muscle defect model

2022 ◽  
Author(s):  
Katie J. Hogan ◽  
Mollie M. Smoak ◽  
Gerry L. Koons ◽  
Marissa R. Perez ◽  
Matthew L. Bedell ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Extracellular Matrix ◽  
Muscle Regeneration ◽  
Rat Skeletal Muscle ◽  
Defect Model ◽  
Decellularized Extracellular Matrix ◽  
Muscle Defect ◽  
Extracellular Matrix Scaffolds

scholarly journals 3D Printing Decellularized Extracellular Matrix to Design Biomimetic Scaffolds for Skeletal Muscle Tissue Engineering

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Silvia Baiguera ◽  
Costantino Del Gaudio ◽  
Paolo Di Nardo ◽  
Vittorio Manzari ◽  
Felicia Carotenuto ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Extracellular Matrix ◽  
3D Printing ◽  
Muscle Regeneration ◽  
Large Scale ◽  
Structural Complexity ◽  
Patient Specific ◽  
Clinical Issue ◽  
Decellularized Extracellular Matrix ◽  
Patient Specific Implants

Functional engineered muscles are still a critical clinical issue to be addressed, although different strategies have been considered so far for the treatment of severe muscular injuries. Indeed, the regenerative capacity of skeletal muscle (SM) results inadequate for large-scale defects, and currently, SM reconstruction remains a complex and unsolved task. For this aim, tissue engineered muscles should provide a proper biomimetic extracellular matrix (ECM) alternative, characterized by an aligned/microtopographical structure and a myogenic microenvironment, in order to promote muscle regeneration. As a consequence, both materials and fabrication techniques play a key role to plan an effective therapeutic approach. Tissue-specific decellularized ECM (dECM) seems to be one of the most promising material to support muscle regeneration and repair. 3D printing technologies, on the other side, enable the fabrication of scaffolds with a fine and detailed microarchitecture and patient-specific implants with high structural complexity. To identify innovative biomimetic solutions to develop engineered muscular constructs for the treatment of SM loss, the more recent (last 5 years) reports focused on SM dECM-based scaffolds and 3D printing technologies for SM regeneration are herein reviewed. Possible design inputs for 3D printed SM dECM-based scaffolds for muscular regeneration are also suggested.

scholarly journals Sca-1 influences the innate immune response during skeletal muscle regeneration

2011 ◽  
Vol 300 (2) ◽  
pp. C287-C294 ◽  
Author(s):  
Kimberly K. Long ◽  
Grace K. Pavlath ◽  
Monty Montano
Keyword(s):  
Skeletal Muscle ◽  
Immune Response ◽  
Extracellular Matrix ◽  
Innate Immunity ◽  
Muscle Regeneration ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Primary Source ◽  
Innate Immune ◽  
Skeletal Muscle Regeneration

Efficient muscle regeneration requires the clearance of dead and dying tissue via phagocytosis before remodeling. We have previously shown that mice lacking stem cell antigen-1 (Sca-1) display a defect in skeletal muscle regeneration characterized by increased fibrosis and decreased turnover of the extracellular matrix. In the present study we demonstrate that Sca-1−/− mice have a defect in their capacity to recruit soluble IgM, and subsequently C3 complement, to damaged muscle. We hypothesize that this defect in recruitment delays or decreases phagocytosis by macrophages, contributing to the previously observed fibrotic phenotype of these mice. As the primary source of soluble IgM is peritoneal B-1a cells, which are a subset of self-renewing B cells, we analyzed this cell population and observed a significant reduction in B-1a cells in Sca-1−/− animals. Interestingly, these mice are protected from ischemia-reperfusion injury, an acute inflammatory reaction also mediated by IgM and C3 complement that has been linked to a deficit in B-1a cells in previous studies. Collectively, these data reveal a novel role for Sca-1 in innate immunity during muscle regeneration and indicate that further elucidation of immuno-myogenic processes will help to better understand and promote muscle regeneration.

Skeletal Muscle Regeneration by the Exosomes of Adipose Tissue-Derived Mesenchymal Stem Cells

2021 ◽  
Vol 43 (3) ◽  
pp. 1473-1488
Author(s):  
Seong-Eun Byun ◽  
Changgon Sim ◽  
Yoonhui Chung ◽  
Hyung Kyung Kim ◽  
Sungmoon Park ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Adipose Tissue ◽  
Muscle Regeneration ◽  
Therapeutic Potential ◽  
Human Adipose Tissue ◽  
Treated Group ◽  
Skeletal Muscle Regeneration ◽  
Biopsy Punch ◽  
Healthy Control ◽  
Muscle Defect

Profound skeletal muscle loss can lead to severe disability and cosmetic deformities. Mesenchymal stem cell (MSC)-derived exosomes have shown potential as an effective therapeutic tool for tissue regeneration. This study aimed to determine the regenerative capacity of MSC-derived exosomes for skeletal muscle regeneration. Exosomes were isolated from human adipose tissue-derived MSCs (AD-MSCs). The effects of MSC-derived exosomes on satellite cells were investigated using cell viability, relevant genes, and protein analyses. Moreover, NOD-SCID mice were used and randomly assigned to the healthy control (n = 4), muscle defect (n = 6), and muscle defect + exosome (n = 6) groups. Muscle defects were created using a biopsy punch on the quadriceps of the hind limb. Four weeks after the surgery, the quadriceps muscles were harvested, weighed, and histologically analyzed. MSC-derived exosome treatment increased the proliferation and expression of myocyte-related genes, and immunofluorescence analysis for myogenin revealed a similar trend. Histologically, MSC-derived exosome-treated mice showed relatively preserved shapes and sizes of the muscle bundles. Immunohistochemical staining revealed greater expression of myogenin and myoblast determination protein 1 in the MSC-derived exosome-treated group. These results indicate that exosomes extracted from AD-MSCs have the therapeutic potential for skeletal muscle regeneration.

Changes of vinculin and extracellular matrix components following blunt trauma to rat skeletal muscle

1993 ◽  
Vol 25 (7) ◽  
pp. 832-840 ◽  
Author(s):  
KATSUYA KAMI ◽  
MITSUHIKO MASUHARA ◽  
HITOSHI KASHIBA ◽  
YOSHINORI KAWAI ◽  
KOICHI NOGUCHI ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Extracellular Matrix ◽  
Blunt Trauma ◽  
Rat Skeletal Muscle ◽  
Extracellular Matrix Components ◽  
Matrix Components

Influence of a dextran derivative on myosin heavy chain expression during rat skeletal muscle regeneration

1995 ◽  
Vol 201 (3) ◽  
pp. 243-246 ◽  
Author(s):  
A. Aamiri ◽  
G.S. Butler-Browne ◽  
I. Martelly ◽  
D. Barritault ◽  
J. Gautron
Keyword(s):  
Skeletal Muscle ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Muscle Regeneration ◽  
Skeletal Muscle Regeneration ◽  
Rat Skeletal Muscle

scholarly journals Tissue specific muscle extracellular matrix hydrogel improves skeletal muscle regeneration in vivo over non-matched tissue source

2020 ◽  
Author(s):  
Jessica L. Ungerleider ◽  
Monika Dzieciatkowska ◽  
Kirk C. Hansen ◽  
Karen L. Christman
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Extracellular Matrix ◽  
Regenerative Medicine ◽  
Expression Analysis ◽  
Muscle Regeneration ◽  
Gene Expression Analysis ◽  
Skeletal Muscle Regeneration ◽  
Cross Sectional ◽  
Tissue Specific

AbstractDecellularized extracellular matrix (ECM) hydrogels present a novel, clinical intervention for a myriad of regenerative medicine applications. The source of ECM is typically the same tissue to which the treatment is applied; however, the need for tissue specific ECM sources has not been rigorously studied. We hypothesized that tissue specific ECM would improve regeneration through preferentially stimulating physiologically relevant processes (e.g. progenitor cell proliferation and differentiation). One of two decellularized hydrogels (tissue specific skeletal muscle or non mesoderm-derived lung) or saline were injected intramuscularly two days after notexin injection in mice (n=7 per time point) and muscle was harvested at days 5 and 14 for histological and gene expression analysis. Both injectable hydrogels were decellularized using the same detergent and were controlled for donor characteristics (i.e. species, age). At day 5, the skeletal muscle ECM hydrogel significantly increased the density of Pax7+ satellite cells in the muscle. Gene expression analysis at day 5 showed that skeletal muscle ECM hydrogels increased expression of genes implicated in muscle contractility. By day 14, skeletal muscle ECM hydrogels improved muscle regeneration over saline and lung ECM hydrogels as shown through a shift in fiber cross sectional area distribution towards larger fibers. This data indicates a potential role for muscle-specific regenerative capacity of decellularized, injectable muscle hydrogels. Further transcriptomic analysis of whole muscle mRNA indicates the mechanism of tissue specific ECM-mediated tissue repair may be immune and metabolism pathway-driven. Taken together, this suggests there is benefit in using tissue specific ECM for regenerative medicine applications.

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