scholarly journals Contribution of CT Features in the Diagnosis of COVID-19

2020 ◽  
Vol 2020 ◽  
pp. 1-16
Author(s):  
Houdong Zuo
Keyword(s):  
World Health ◽  
Rt Pcr ◽  
Chain Reaction ◽  
The World ◽  
Novel Virus ◽  
Polymerase Chain ◽  
Novel Coronavirus ◽  
Health Organization ◽  
Diagnostic Criterion ◽  
Ct Features

The outbreak of novel coronavirus disease 2019 (COVID-19) first occurred in Wuhan, Hubei Province, China, and spread across the country and worldwide quickly. It has been defined as a major global health emergency by the World Health Organization (WHO). As this is a novel virus, its diagnosis is crucial to clinical treatment and management. To date, real-time reverse transcription-polymerase chain reaction (RT-PCR) has been recognized as the diagnostic criterion for COVID-19. However, the results of RT-PCR can be complemented by the features obtained in chest computed tomography (CT). In this review, we aim to discuss the diagnosis and main CT features of patients with COVID-19 based on the results of the published literature, in order to enhance the understanding of COVID-19 and provide more detailed information regarding treatment.

Multisystem Inflammatory Syndrome in Infant with Negative SARS-CoV-2 RT-PCR and Antibodies

2021 ◽  
pp. 35-39
Author(s):  
Hanna Sahhar ◽  
Karly Derwitz ◽  
Erica Rubin
Keyword(s):  
Polymerase Chain Reaction ◽  
Severe Acute Respiratory Syndrome ◽  
Early Diagnosis ◽  
World Health ◽  
Rt Pcr ◽  
Chain Reaction ◽  
The World ◽  
Polymerase Chain ◽  
Health Organization ◽  
Inflammatory Syndrome

Since the declaration of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic in March 2020 by the World Health Organization (WHO), there has been an emergence of a new syndrome termed multisystem inflammatory syndrome in children (MIS-C) associated with COVID-19. MIS-C is defined by the presence of fever, systemic inflammation and multiorgan dysfunction in association with SARS-CoV-2 infection or COVID-19 exposure. Knowledge of this syndrome’s presentation and pathophysiology is constantly evolving as more cases are reported in the literature. This case identifies a 3-month-old patient who tested negative for SARS-CoV-2 antigen, reverse transcriptase polymerase chain reaction (RT-PCR) and antibodies but qualified for MIS-C diagnosis. To the best of our knowledge and through extensive research at the time of diagnosing and reporting this condition to the healthcare authorities, we report the youngest pediatric patient with MIS-C diagnosis. We document this case to contribute to further understanding the variable manifestations of MIS-C and the importance of early diagnosis and treatment with intravenous immunoglobulin (IVIG).

scholarly journals A comprehensive review on epidemiology, aetiopathogenesis, diagnosis and treatment of the novel coronavirus syndrome – COVID-19

2020 ◽  
Vol 2 (2) ◽  
pp. 110-123
Author(s):  
Kulvinder Kochar Kaur ◽  
◽  
Gautam Allahbadia ◽  
Mandeep Singh ◽  
◽  
...  
Keyword(s):  
Respiratory Illness ◽  
World Health ◽  
The Novel ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Rapid Spread ◽  
Polymerase Chain ◽  
Novel Coronavirus ◽  
Health Organization ◽  
Severe Respiratory Illness

Since the outbreak of the novel Coronavirus in December 2019 in Wuhan China, this novel Coronavirus disease(COVID-19) has spread worldwide taking not only epidemic proportions but with its rapid spread world health organization(WHO) was forced to declare it as a pandemic. The Severe Acute respiratory syndrome (SARS)-Coronavirus (CoV2) virus is responsible for clusters of severe respiratory illness that simulates acute respiratory syndrome that was what was initially. It is thought although that it is equivalent to the high altitude pulmonary oedema (HAPE), showing glass ground opacities in lungs. More experience is getting acquired with changes in treatment approaches from PEEP to avoid intubation and just ensure oxygen levels maintained. Human to human transmission through droplets, contaminated hands as well as surfaces, has been revealed with an incubation period varying from 2-14 days. Early diagnosis using reverse transcription polymerase chain reaction (RT-PCR) or computed tomography (CT) scan chest, quarantine, as well as supportive treatment are necessary for getting a cure. In this review we have tried to analyze the epidemiology, diagnosis, isolation, and treatment, including antiviral drugs like remdesivir, favipiravir, chloroquine and hydroxychloroquine, corticosteroids, antibiotics, and ivermectin. With 3 successful cases of convalescent plasma achieved in USA, trials going on in India along with vaccines are also detailed in this article.

scholarly journals The Expression of miR-155-5p and Local Matrix Gla Protein in Meningiomas

2021 ◽  
Vol 29 (3) ◽  
pp. 299-306
Author(s):  
Simona Roxana Gheorghe ◽  
Cătălin Marian ◽  
Ligia Gabriela Tătăranu ◽  
Anica Dricu ◽  
Cees Vermeer ◽  
...  
Keyword(s):  
Messenger Rna ◽  
Molecular Classification ◽  
Micro Rna ◽  
Matrix Gla Protein ◽  
World Health ◽  
Ectopic Calcification ◽  
Chain Reaction ◽  
The World ◽  
Polymerase Chain ◽  
Health Organization

Abstract Meningiomas are classified by the World Health Organization (WHO) in three grades, based on morphological features. Independent of this grading, the presence of calcification in meningiomas influences their growth rate. The messenger RNA of matrix Gla protein (MGP), an extra-hepatic protein with different conformations involved in the homeostasis of ectopic calcification has been found in meningiomas and was shown to be regulated in breast cancer by miR-155-5p, a specific micro RNA. Therefore, we investigated the expression of miR-155-5p and its relationship with local MGP conformations in different grade meningiomas. According to the WHO classification, our 41 samples of meningiomas were stratified in groups WHO I and WHO II. Using real time polymerase chain reaction, we observed a higher miR-155-5p expression in group WHO I versus group WHO II [with a fold change (FC) of 3.83, p=0.027)]. Moreover, the expression of miR-155-5p was higher in calcified tumors compared to non-calcified tumors in all samples (FC=3.01, p=0.047) and in group WHO I (FC=3.65, p=0.048). Utilizing immunohistochemistry, we determined the concurrent presence of all MGP conformations in calcified meningiomas. This study was the first to establish higher miR-155-5p expression in grade WHO I and calcified meningiomas, which could improve molecular classification and targeted therapy and also the presence of all MGP conformations in calcified meningiomas, confirming the existence of an anti-calcification mechanism in meningiomas..

scholarly journals Persistent Detection and Infectious Potential of SARS-CoV-2 Virus in Clinical Specimens from COVID-19 Patients

Viruses ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1384
Author(s):  
Michael Zapor
Keyword(s):  
Pcr Amplification ◽  
World Health ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Distinctive Features ◽  
Clinical Specimens ◽  
Polymerase Chain ◽  
Health Organization ◽  
Infectious Potential ◽  
Infective Potential

The Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2) that emerged in December 2019 as the causative agent of Coronavirus 2019 (COVID-19) and was declared a pandemic by the World Health Organization in March 2020 has several distinctive features, including extensive multiorgan involvement with a robust systemic inflammatory response, significant associated morbidity and mortality, and prolonged persistence of viral RNA in the clinical specimens of infected individuals as detected by Reverse Transcription Polymerase Chain Reaction (RT-PCR) amplification. This review begins with an overview of SARS-CoV-2 morphology and replication and summarizes what is known to date about the detection of the virus in nasal, oropharyngeal, and fecal specimens of patients who have recovered from COVID-19, with a focus on the factors thought to contribute to prolonged detection. This review also provides a discussion on the infective potential of this material from asymptomatic, pre-symptomatic, and convalescing individuals, to include a discussion of the relative persistence and infectious potential of virus in clinical specimens recovered from pediatric COVID-19 patients.

scholarly journals Vulvar Tuberculosis—A Rare Manifestation of the Disease

2019 ◽  
Vol 41 (09) ◽  
pp. 575-578 ◽  
Author(s):  
Cristina Bragança ◽  
Inês Gonçalves ◽  
Luísa Guerreiro ◽  
Maria Janeiro
Keyword(s):  
Rare Case ◽  
Causes Of Death ◽  
World Health ◽  
Histological Findings ◽  
Chain Reaction ◽  
Rare Manifestation ◽  
The World ◽  
History Of ◽  
Polymerase Chain ◽  
Health Organization

AbstractTuberculosis is an infectious disease caused by Mycobacterium tuberculosis. According to data from the World Health Organization, this disease remains one of the leading causes of death worldwide. Although it most commonly affects the lungs, tuberculosis can compromise any organ. The present study reports a rare case of vulvar tuberculosis in a postmenopausal woman with a history of asymptomatic pulmonary and pleural tuberculosis, with no prior documented contact with the bacillus. Diagnosis was based on vulvar lesion biopsies, with histological findings suggestive of infection and isolation of M. tuberculosis by microbiological culture and polymerase chain reaction (PCR) essays. The lesions reverted to normal after tuberculostatic therapy.

scholarly journals Methods of Diagnosis a Preferential Advantage in COVID-19: A Mini Review

2021 ◽  
pp. 173-179
Author(s):  
Neha Saini ◽  
Prem Pandey ◽  
Mandar Shirolkar ◽  
Atul Kulkarni
Keyword(s):  
Polymerase Chain Reaction ◽  
Severe Acute Respiratory Syndrome ◽  
Review Article ◽  
Diagnostic Methods ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Health Crisis ◽  
The World ◽  
Polymerase Chain ◽  
Novel Coronavirus

Humanity is going through never seen before health crisis due to the outbreak of novel coronavirus or Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). There are 24.02 million cases and 0.82 million deaths worldwide as of 26th August 2020 due to deadly infection of COVID-19. The disease has been spreading exponentially (R-naught number: 3) and has challenged even the best healthcare infrastructure in the world. With the progression of the disease, the countries shifted the focus from cure to diagnosis and containment to flatten the curve. The review shows that the disease is spreading exponentially while the resources are still limited. We focus upon the probable vectors of the virus, different diagnostic methods with advantages & limitations, and the way forward. This review article covers the different diagnostic methods with more advantages, limitations, and the future sneak-peek into the forthcoming developments for the diagnostic processes such as RT-PCR (Reverse Transcription Polymerase chain reaction).

scholarly journals An atypical case: RT-PCR-negative, sero-positive covid-19 patient

Pneumologia ◽  
2020 ◽  
Vol 69 (2) ◽  
pp. 107-114
Author(s):  
William Suriady ◽  
Andika Chandra Putra ◽  
Wiwien Heru Wiyono ◽  
Mohammad Fahmi Alatas ◽  
Bettia Bermawi ◽  
...  
Keyword(s):  
False Negative ◽  
Severe Pneumonia ◽  
World Health ◽  
Diagnostic Process ◽  
The Novel ◽  
Rt Pcr ◽  
Atypical Case ◽  
Polymerase Chain ◽  
Novel Coronavirus ◽  
Health Organization

Abstract The novel coronavirus disease-2019 (COVID-19), caused by the severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), has become a public health emergency of international concern. The first confirmed COVID-19 case in Indonesia was announced on 2 March 2020, and later on, 11,192 confirmed cases were reported as of 3 May. The World Health Organization has stated that performing a real-time reverse transcription–polymerase chain reaction (RT-PCR) specific for SARS-CoV-2 on specimens from the upper and the lower respiratory tracts, especially nasopharyngeal and oropharyngeal swabs, is the standard diagnostic procedure for COVID-19. In Indonesia, we also use other diagnostic tests, such as rapid antibody tests specific for SARS-CoV-2. Herein, we report an atypical case of COVID-19 and describe the diagnostic process, the clinical course, with progression to severe pneumonia on Week 3 of illness and the case management. We also try to highlight the possibility of false-negative RT-PCR tests.

scholarly journals The Value of Pre-operative Testing for COVID-19 in Urological Patients

2020 ◽  
Vol 3 (3) ◽  
pp. e29-e34
Author(s):  
Vasileios Bonatsos ◽  
Asif Raza
Keyword(s):  
Gold Standard ◽  
Diagnostic Tests ◽  
Acute Infection ◽  
World Health Organisation ◽  
World Health ◽  
Rt Pcr ◽  
Chain Reaction ◽  
The World ◽  
Polymerase Chain ◽  
Pcr Assays

According to the World Health Organisation there have been 30,055,710 confirmed COVID-19 cases and 933,433 confirmed deaths across 216 countries globally. The availability of the complete SARS-CoV-2 genome relatively early in the epidemic has enabled the development of tests for the diagnosis of COVID-19. There are two broad categories of SARS-CoV-2 diagnostic tests currently in use or development: (1) Real-time reverse transcriptase polymerase chain reaction (RT-PCR) tests and (2) serology tests. RT-PCR is considered the gold standard and preferred method of diagnosis of acute infection. There is, however, a plethora of laboratory-developed and commercial RT-PCR assays with different gene targets. We discuss the value of pre-operative testing for COVID-19 before urological surgery.

scholarly journals Combating the Coronavirus Pandemic: Early Detection, Medical Treatment, and a Concerted Effort by the Global Community

Research ◽  
2020 ◽  
Vol 2020 ◽  
pp. 1-35 ◽  
Author(s):  
Zichao Luo ◽  
Melgious Jin Yan Ang ◽  
Siew Yin Chan ◽  
Zhigao Yi ◽  
Yi Yiing Goh ◽  
...  
Keyword(s):  
Respiratory Illness ◽  
Viral Disease ◽  
Disease Diagnosis ◽  
World Health ◽  
Rt Pcr ◽  
Sudden Loss ◽  
Difficulty Breathing ◽  
Polymerase Chain ◽  
Novel Coronavirus ◽  
Health Organization

The World Health Organization (WHO) has declared the outbreak of 2019 novel coronavirus, known as 2019-nCoV, a pandemic, as the coronavirus has now infected over 2.6 million people globally and caused more than 185,000 fatalities as of April 23, 2020. Coronavirus disease 2019 (COVID-19) causes a respiratory illness with symptoms such as dry cough, fever, sudden loss of smell, and, in more severe cases, difficulty breathing. To date, there is no specific vaccine or treatment proven effective against this viral disease. Early and accurate diagnosis of COVID-19 is thus critical to curbing its spread and improving health outcomes. Reverse transcription-polymerase chain reaction (RT-PCR) is commonly used to detect the presence of COVID-19. Other techniques, such as recombinase polymerase amplification (RPA), loop-mediated isothermal amplification (LAMP), clustered regularly interspaced short palindromic repeats (CRISPR), and microfluidics, have allowed better disease diagnosis. Here, as part of the effort to expand screening capacity, we review advances and challenges in the rapid detection of COVID-19 by targeting nucleic acids, antigens, or antibodies. We also summarize potential treatments and vaccines against COVID-19 and discuss ongoing clinical trials of interventions to reduce viral progression.

scholarly journals SARS-CoV-2; What We Know so far

2020 ◽  
Vol 23 (7) ◽  
pp. 498-502 ◽  
Author(s):  
Huda Fatima Rajani ◽  
Fatima Ahmed Alshaikh ◽  
Amir Anushiravani
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Diagnostic Methods ◽  
World Health ◽  
Chain Reaction ◽  
The World ◽  
Recent Outbreak ◽  
Control And Prevention ◽  
Polymerase Chain ◽  
Precautionary Measures ◽  
Health Organization

A recent outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected more than 1100000 (April 5, 2020) individuals worldwide and is spreading rapidly. The virus is reported to be derived from bats and the infection was first reported in China. Similar to the severe acute respiratory syndrome and the Middle East respiratory syndrome coronaviruses, it is responsible for respiratory tract infection. Real time polymerase chain reaction and radiography are the two main diagnostic methods. Guidelines from the Center for Disease Control and Prevention and the World Health Organization (WHO) should be followed for diagnostic and precautionary measures. Treatment of the infection is still not available; however, antivirals are under clinical trials.

Sign in / Sign up

Export Citation Format

Share Document