scholarly journals The Role of Epoxyeicosatrienoic Acids in Diabetes Mellitus-Induced Impaired Vascular Relaxation of Aortic Rings in Ovariectomized Sprague-Dawley Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Dragan Manojlović ◽  
Ana Stupin ◽  
Anita Matić ◽  
Zrinka Mihaljević ◽  
Sanja Novak ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Mrna Expression ◽  
Vascular Function ◽  
Diabetic Rats ◽  
Ovariectomized Rats ◽  
Aortic Tissue ◽  
Sprague Dawley ◽  
Sd Rats

Aim. The present study was aimed at determining if type 1 diabetes mellitus (DM) affects vascular function and at elucidating the mechanisms mediating vasorelaxation in both nonovariectomized and ovariectomized Sprague-Dawley (SD) rats. Materials and Methods. Eighty female SD rats were divided into four groups: nonovariectomized healthy (non-OVX-CTR) and diabetic (non-OVX-DM) rats and ovariectomized healthy (OVX-CTR) and diabetic (OVX-DM) rats. Bilateral ovariectomy was performed at the age of 5 weeks, and type 1 DM was induced by streptozotocin at the age of 6 weeks. At the age of 12 weeks, acetylcholine-induced relaxation (AChIR) was assessed in aortic rings in the absence/presence of L-NAME, Indomethacin, and MS-PPOH. Aortic tissue mRNA expression of eNOS, iNOS, COX-1, COX-2, thromboxane synthase 1 (TBXAS1), CYP4A1, CYP4A3, and CYP2J3, as well as plasma oxidative stress, was measured. Results. AChIR did not differ in non-OVX-DM rats compared to non-OVX-CTR ones. AChIR was significantly reduced in the OVX-DM group compared to the OVX-CTR group. MS-PPOH did not reduce AChIR in OVX-DM rats as it did in OVX-CTR ones. CYP4a3 mRNA expression in OVX-DM rats was significantly lower compared to that in the OVX-CTR group. Conclusions. Female sex hormones may protect vasorelaxation in type 1 diabetic rats. Type 1 diabetes impairs vasorelaxation in response to ACh in ovariectomized rats (but not in nonovariectomized rats) by affecting vasorelaxation pathways mediated by EETs.

scholarly journals Effect of moderate exercise on renal changes and oxidative stress in ovariectomized rats with type 1 diabetes mellitus

2019 ◽  
Vol 6 (13) ◽  
pp. 331-345 ◽  
Author(s):  
Mayane Oliveira Rebouças da Silveira ◽  
Liliany Souza de Brito Amaral ◽  
Samira Itana de Souza ◽  
Halanna Rocha Ferraz ◽  
Jéssica Alves Dias ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Renal Function ◽  
Aerobic Exercise ◽  
Type 1 Diabetes Mellitus ◽  
Diabetic Rats ◽  
Ovariectomized Rats ◽  
And Oxidative Stress

This study evaluated the aerobic exercise effects of moderate and progressive intensity on renal function and structure, and oxidative stress in ovariectomized rats with type 1 diabetes mellitus induced by streptozotocin (STZ). Eighteen Wistar rats were divided into 3 groups: OSC - ovariectomized and sedentary control rats; OSD - ovariectomized and sedentary diabetic rats; and OTD - ovariectomized and trained diabetic rats. After induction of diabetes, the OTD group was submitted to eight weeks of exercise. Twenty-four hours after the last training session urine samples were collected. Blood samples and kidneys were collected after euthanasia for renal function analysis, histology, morphometry and oxidative stress. Our results have shown a reduction of the weight gain, increase of kidney weight and postprandial glycemia in diabetic rats. However, exercise decreased glycosuria and prevented the proteinuria in OTD group rats. Focal segmental glomerulosclerosis (FSGS), juxtamedullary glomerular tuft area, tubulointerstitial lesions (TIL), brush border loss and tubular cell debridement were reduced in OTD rats. In addition, exercise training decreased urinary and plasma concentrations of thiobarbituric acid reactive substance (TBARS). Our results demonstrate the beneficial effect of progressive aerobic exercise on proteinuria, glycosuria, and renal structure in ovariectomized diabetic rats, which may be mediated in part by reduction of oxidative stress.

scholarly journals Biological Activities of Chinese Propolis and Brazilian Propolis on Streptozotocin-Induced Type 1 Diabetes Mellitus in Rats

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Wei Zhu ◽  
Minli Chen ◽  
Qiyang Shou ◽  
Yinghua Li ◽  
Fuliang Hu
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Type 1 Diabetes Mellitus ◽  
Biological Activities ◽  
Total Cholesterol Level ◽  
Diabetic Rats ◽  
Sprague Dawley ◽  
Brazilian Propolis ◽  
Chinese Propolis

Propolis is a bee-collected natural product and has been proven to have various bioactivities. This study tested the effects of Chinese propolis and Brazilian propolis on streptozotocin-induced type 1 diabetes mellitus in Sprague-Dawley rats. The results showed that Chinese propolis and Brazilian propolis significantly inhibited body weight loss and blood glucose increase in diabetic rats. In addition, Chinese propolis-treated rats showed an 8.4% reduction of glycated hemoglobin levels compared with untreated diabetic rats. Measurement of blood lipid metabolism showed dyslipidemia in diabetic rats and Chinese propolis helped to reduce total cholesterol level by 16.6%. Moreover, oxidative stress in blood, liver and kidney was improved to various degrees by both Chinese propolis and Brazilian propolis. An apparent reduction in levels of alanine transaminase, aspartate transaminase, blood urea nitrogen and urine microalbuminuria-excretion rate demonstrated the beneficial effects of propolis in hepatorenal function. All these results suggested that Chinese propolis and Brazilian propolis can alleviate symptoms of diabetes mellitus in rats and these effects may partially be due to their antioxidant ability.

scholarly journals Nutrient Induced Type 2 and Chemical Induced Type 1 Experimental Diabetes Differently Modulate Gastric GLP-1 Receptor Expression

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Olga Bloch ◽  
Efrat Broide ◽  
Gilad Ben-Yehudah ◽  
Dror Cantrell ◽  
Haim Shirin ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Experimental Diabetes ◽  
High Energy ◽  
Diabetic Rats ◽  
Receptor Expression ◽  
Glandular Stomach ◽  
Gastric Glands ◽  
Sd Rats ◽  
Neck Area

T2DM patients demonstrate reduced GLP-1 receptor (GLP-1R) expression in their gastric glands. Whether induced T2DM and T1DM differently affect the gastric GLP-1R expression is not known. This study assessed extrapancreatic GLP-1R system in glandular stomach of rodents with different types of experimental diabetes. T2DM and T1DM were induced inPsammomys obesus(PO) by high-energy (HE) diet and by streptozotocin (STZ) in Sprague Dawly (SD) rats, respectively. GLP-1R expression was determined in glandular stomach by RT PCR and immunohistomorphological analysis. The mRNA expression and cellular association of the GLP-1R in principal glands were similar in control PO and SD rats. However, nutrient and chemical induced diabetes resulted in opposite alterations of glandular GLP-1R expression. Diabetic PO demonstrated increased GLP-1R mRNA expression, intensity of cellular GLP-1R immunostaining, and frequency of GLP-1R positive cells in the neck area of principal glands compared with controls. In contrast, SD diabetic rats demonstrated decreased GLP-1 mRNA, cellular GLP-1R immunoreactivity, and frequency of GLP-1R immunoreactive cells in the neck area compared with controls. In conclusion, nutrient and chemical induced experimental diabetes result in distinct opposite alterations of GLP-1R expression in glandular stomach. These results suggest that induced T1DM and T2DM may differently modulate GLP-1R system in enteropancreatic axis.

Lipid peroxidation and impaired vascular function in patients with type 1 diabetes mellitus

2019 ◽  
Vol 150 (3) ◽  
pp. 525-529 ◽  
Author(s):  
Tomáš Pelcl ◽  
Jan Škrha ◽  
Jan Šoupal ◽  
Milan Flekač ◽  
Petr Kačer ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Lipid Peroxidation ◽  
Type 1 Diabetes ◽  
Type 1 Diabetes Mellitus ◽  
Vascular Function

scholarly journals DNA methylation and mRNA expression of HLA-DQA1 alleles in type 1 diabetes mellitus

Immunology ◽  
2016 ◽  
Vol 148 (2) ◽  
pp. 150-159 ◽  
Author(s):  
Pavel Cepek ◽  
Marta Zajacova ◽  
Anna Kotrbova-Kozak ◽  
Elena Silhova ◽  
Marie Cerna
Keyword(s):  
Diabetes Mellitus ◽  
Dna Methylation ◽  
Type 1 Diabetes ◽  
Type 1 Diabetes Mellitus ◽  
Mrna Expression ◽  
Hla Dqa1

scholarly journals Preventive effect of exercise training on diabetic kidney disease in ovariectomized rats with type 1 diabetes

2019 ◽  
Vol 244 (9) ◽  
pp. 758-769 ◽  
Author(s):  
Cláudia Silva Souza ◽  
Bianca Silva de Sousa Oliveira ◽  
Geovanildo Nascimento Viana ◽  
Thiago Macêdo Lopes Correia ◽  
Ana Carolina de Bragança ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Renal Function ◽  
Physical Exercise ◽  
Diabetic Kidney Disease ◽  
Renal Cortex ◽  
Diabetic Rats ◽  
Ovariectomized Rats ◽  
Renal Diseases ◽  
Tgf Β1

This study evaluated the effects of moderate regular physical exercise on lipid peroxidation, inflammation and renal function and structure in streptozotocin (STZ)-induced ovariectomized diabetic rats. Twenty-four Wistar rats were anesthetized and submitted to bilateral ovariectomy. The rats were divided into four experimental groups: ovariectomized control (OC), ovariectomized trained control (OTC), ovariectomized diabetic (OD), ovariectomized trained diabetic (OTD). The OTD and OTC groups were submitted to treadmill running for four weeks before to diabetes mellitus (DM) induction with STZ (40 mg/kg, i.v) or vehicle injection, respectively, and continued the training for another eight weeks after the injections. Urine samples were collected 24 h after the last training session, and blood samples and kidneys were collected after euthanasia. Our results showed that physical exercise attenuated the loss of body weight and postprandial glucose and improved physical capacity in the OTD group. Lower serum creatinine levels, glycosuria, and proteinuria were also observed in this group. There was no difference in 17β-estradiol levels between the experimental groups. The glomerular and tubulointerstitial changes were attenuated in the OTD group. Expressions of macrophages, lymphocytes, fibronectin, TGF-β1, and NF-κB were reduced in the glomeruli of OTD group. Exercise also reduced macrophages, lymphocytes, and TGF-β1 expression in the tubulointerstitial compartment of the renal cortex of this group. In addition, increased levels of TBARS in the renal cortex of diabetic rats were prevented by exercise in the OTD group. In conclusion, our study shows that physical exercise exerted a renoprotective effect against the progression of diabetic kidney disease in ovariectomized rats. Impact statement To date, no studies have been found evaluating the effects of physical exercise on renal function and structure changes in ovariectomized rats with type 1 diabetes. Therefore, this work emerges with an important tool for strengthening and expanding innovative research on exercise with potential for the prevention of renal diseases in ovariectomized diabetic rats, and future development of studies that seek to increase scientific knowledge about the beneficial effects of physical exercise on renal diseases in humans.

scholarly journals Atazanavir improves cardiometabolic measures but not vascular function in patients with long-standing type 1 diabetes mellitus

2015 ◽  
Vol 52 (4) ◽  
pp. 709-715 ◽  
Author(s):  
Jessica Milian ◽  
Allison B. Goldfine ◽  
Jonah P. Zuflacht ◽  
Caitlin Parmer ◽  
Joshua A. Beckman
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Type 1 Diabetes Mellitus ◽  
Vascular Function
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