scholarly journals Identification and Characterization of Key Chemical Constituents in Processed Gastrodia elata Using UHPLC-MS/MS and Chemometric Methods

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Xide Ye ◽  
Yanhong Wang ◽  
Jianping Zhao ◽  
Mei Wang ◽  
Bharathi Avula ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Raw Material ◽  
Gastrodia Elata ◽  
Peak Areas ◽  
Marker Compounds ◽  
Fragmentation Patterns ◽  
Traditional Processing ◽  
Identification And Characterization ◽  
Processing Mechanism

Gastrodia elata Blume belongs to the Orchidaceae family. G. elata is often processed when used in traditional Chinese medicine (TCM). In the current study, a traditional processing method, known as “Jianchang Bang,” was applied. Steamed and dried (S&D) G. elata was processed with ginger juice for up to 5 days (GEP5D). An UHPLC-MS/MS combined with a chemometric method was developed for the analysis of processed G. elata along with the raw material as well as steamed and dried G. elata. As a result, the primary marker compounds were identified with the aid of TOF-MS and MS/MS analyses. Compared with the raw material of G. elata with GEP5D, three new parishin-type compounds were identified according to their retention time, accurate mass, and fragmentation patterns. The chromatographic peak areas for marker compounds, including S-(gastrodin)-glutathione, S-(4-hydroxybenzylamine)-glutathione, and parishin-type compounds, changed significantly. This result indicated that by applying the “Jianchang Bang” method, changes in chemical composition in G. elata contents were observed. The study also demonstrated that chemometric analysis is helpful in understanding the processing mechanism and will provide scientific support for the clinical application of G. elata.

Identification and Characterization of Lipopeptide Biosurfactant Producing Microbacterium sp Isolated from Brackish River Water

2020 ◽  
Vol 20 (24) ◽  
pp. 2221-2234
Author(s):  
Anuraj Nayarisseri ◽  
Ravina Khandelwal ◽  
Sanjeev Kumar Singh
Keyword(s):  
Surface Tension ◽  
River Water ◽  
Raw Material ◽  
Solvent Recovery ◽  
Oil Degradation ◽  
Microbial Remediation ◽  
Lipopeptide Biosurfactant ◽  
Identification And Characterization ◽  
Isolated Species

Background: Bioremediation has taken its call for removing pollutants for years. The oilcontaminated surroundings are majorly hazardous for sustaining life, but a great contribution to nature in the form of microorganisms. The complex carbon-hydrogen chain has served as classic raw material to chemical industries, which has perked up the hydrocarbon waste. Microbial remediation has been thus, focused to deal with the lacuna, where the new addition to this category is Microbacterium species. Objectives: The identification and characterization of lipopeptide biosurfactant producing Microbacterium spp. isolated from brackish river water. Methods: The strain was isolated from an oil-contaminated lake. The strain was tested with all the other isolated species for oil degradation using screening protocols such as haemolysis, oil spread assay, BATH, E24, etc. The produced biosurfactant was extracted by acid precipitation, followed by solvent recovery. The strain with maximum potential was sequenced and was subjected to phylogeny assessment using in silico tools. Results: Novel Microbacterium species produce the extracellular biosurfactant. The surface tension of Microbacterium was found to be 32mN/m, indicates its powerful surface tension-reducing property. The strain was optimized for the production of biosurfactant and the best results were obtained with sucrose (2%) and yeast extract (3%) medium at 7 pH and 40°C temperature. Conclusion: The isolate was confirmed to be a novel Microbacterium species that could produce 0.461 gm biosurfactant in 100 ml of the medium throughout a life cycle and novel strain of isolate was deposited to NCBI as Microbacterium spp. ANSKSLAB01 using an accession number: KU179507.

Sign in / Sign up

Export Citation Format

Share Document