scholarly journals Electroacupuncture Improves Antidepressant Effects in CUMS Rats by Protecting Hippocampal Synapse and Mitochondrion: An Ultrastructural and iTRAQ Proteomic Study

2019 ◽  
Vol 2019 ◽  
pp. 1-11
Author(s):  
Jialing Zhang ◽  
Zhinan Zhang ◽  
Jiping Zhang ◽  
Zheng Zhong ◽  
Zengyu Yao ◽  
...  
Keyword(s):  
Molecular Mechanism ◽  
Complementary Therapy ◽  
Therapeutic Effects ◽  
Absolute Quantitation ◽  
Ultrastructure Study ◽  
Mitochondrial Functions ◽  
Study Results ◽  
Proteomics Approach ◽  
Proteomic Study ◽  
Isobaric Tag

Electroacupuncture (EA) is considered a complementary therapy for depression. Trials also found that EA has additive benefits when combined with medication compared with medication alone. It is revealed that EA restores altered hippocampal synaptic plasticity in depressed brain. But precise molecular mechanism is poorly understood. Here, we evaluated the therapeutic effects of EA and EA combined with selective serotonin reuptake inhibitor (SSRI) on depressed (CUMS) rats. Then a new proteomics approach, isobaric tag for relative and absolute quantitation (iTRAQ), was used to explore the differential expressed synaptic protein in hippocampus between CUMS and EA-treated rats to identify the possible target molecular mechanism of its effects. We found that EA had additive benefit against depressive behaviors when combined with SSRI. Ultrastructure study on neuron showed significant change in postsynapse density (PSD) and mitochondrion. Through iTRAQ, it is found that synaptic and mitochondrial proteins were significantly changed after EA, consisting with ultrastructure study results. These findings suggest that EA improves antidepressant performance in depressed rats through protecting synaptic and mitochondrial functions in hippocampus.

scholarly journals Exploration of Molecular Mechanism of Traditional Chinese Tuina Therapy for Insomnia by Proteomics Analysis

2021 ◽  
Author(s):  
Jing Zhou ◽  
Biwei Cao ◽  
Meng Wei ◽  
Yuan Xiong ◽  
Wan Liu ◽  
...  
Keyword(s):  
Molecular Mechanism ◽  
Treated Patient ◽  
Therapy Group ◽  
Neurological Diseases ◽  
Common Mental Disorder ◽  
Enzyme Linked Immunosorbent Assay ◽  
Therapeutic Effects ◽  
Physical And Mental Health ◽  
Absolute Quantitation ◽  
Related Proteins

Abstract Insomnia, one of the most common mental disorder, not only affects the quality of life, but also damages physical and mental health. Therefore, it is very necessary to explore the molecular mechanism of insomnia and find some suitable treatments. At present, methods of using drugs to treat insomnia is not satisfactory due to lack of evidences and side effects. Hence, development of non-drug treatments is particularly important. Tuina, a Chinese massage method, has achieved certain therapeutic effects on injuries, rheumatism, neurological diseases and other types of diseases. We have treated patient with insomnia by Tuina, and obtained therapeutic effects indeed. In the current study, we used iTRAQ (isobaric Tags for Relative and Absolute Quantitation) quantitative proteomics to analyze plasma samples taken from the healthy control (HC) group, insomnia patients group (before Tuina treatment, BTT) and insomnia-therapy group (after Tuina treatment, ATT) to identify the molecular correlation of insomnia. In BTT vs HC, we found that the expression of many immune-related and stress-related proteins were out of control, and it revealed that Tuina had the capacities to regulate expression of immune-related and stress-related proteins in ATT vs BTT, suggesting that Tuina may improve insomnia by regulating immune-related and stress-related proteins. The proteomics verification results had been verified by commercial ELISA(Enzyme linked immunosorbent assay. All in all,Our study not only found a good way to treat insomnia, but also provided a research foundation for improving insomnia.

scholarly journals Investigation into the molecular mechanism of the antiapoptotic functions of CTCF in breast cancer cells using a proteomics approach

2008 ◽  
Vol 10 (S2) ◽  
Author(s):  
CF Méndez-Catalá ◽  
I Cherhukhin ◽  
F Docquier ◽  
D Farrar ◽  
E Pugacheva ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Molecular Mechanism ◽  
Breast Cancer Cells ◽  
Proteomics Approach

scholarly journals Unraveling the neuroprotective effect of Tinospora cordifolia in Parkinsonian mouse model through proteomics Approach.

2021 ◽  
Author(s):  
Hareram Birla ◽  
Chetan Keswani ◽  
Saumitra Sen Singh ◽  
Walia Zahra ◽  
Hagera Dilnashin ◽  
...  
Keyword(s):  
Treatment Group ◽  
Mouse Model ◽  
Molecular Mechanisms ◽  
Neuroprotective Effect ◽  
Mitochondrial Gene ◽  
Tinospora Cordifolia ◽  
Therapeutic Effects ◽  
Proteomics Data ◽  
Proteomics Approach ◽  
Multiple Drugs

Abstract Stress-induced dopaminergic (DAergic) neuronal death in the midbrain region is the primary cause of Parkinson’s disease (PD). From the discovery of L-dopa, multiple drugs were discovered to improve lifestyle of PD patients, but they failed due to their multiple side effects. Tinospora cordifolia (Tc), a medicinal herb has been used in traditional medicines to treat neurodegenerative diseases. In our previous study, the neuroprotective role of Tc against MPTP intoxicated Parkinsonian mice was reported. Here, we further explore the neuroprotective molecular mechanisms of Tc in Rotenone (ROT) intoxicated mouse model through proteomics approach. Mice were pretreated with Tc extract by oral administration, followed by ROT-intoxication. Behavioral tests were performed to check motor functions of mice. Protein was isolated, and label free quantification (LFQ) was carried out to identify differentially expressed protein (DEPs) in control vs. PD and PD vs. treatment group. In this study, we report 800 DEPs in control vs. PD and 133 in PD vs. Treatment group. In silico tools clearly demonstrate significant enrichment of biochemical and molecular pathways with DEPs which are known to be important for PD progression including mitochondrial gene expression, PD pathways, TGF-β signaling, Alzheimer’s disease etc. This results were further validated by qRT-PCR and found that the expression of target gene were identical to the proteomics data. This study provides a novel insight for the disease progression as well new therapeutic tagets. More importantly, it demonstrates that Tc exerts the therapeutic effects through the regulation of multiple pathways to protect DAergic neurons.

scholarly journals Systems Pharmacological Approach to Investigate the Mechanism of Acori Tatarinowii Rhizoma for Alzheimer’s Disease

2018 ◽  
Vol 2018 ◽  
pp. 1-20 ◽  
Author(s):  
Zhenyan Song ◽  
Fang Yin ◽  
Biao Xiang ◽  
Bin Lan ◽  
Shaowu Cheng
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Enrichment Analysis ◽  
Pathway Enrichment Analysis ◽  
Therapeutic Effects ◽  
Biological Processes ◽  
Pathway Enrichment ◽  
Study Results ◽  
Target Network ◽  
Anti Inflammatory

In traditional Chinese medicine (TCM), Acori Tatarinowii Rhizoma (ATR) is widely used to treat memory and cognition dysfunction. This study aimed to confirm evidence regarding the potential therapeutic effect of ATR on Alzheimer’s disease (AD) using a system network level based in silico approach. Study results showed that the compounds in ATR are highly connected to AD-related signaling pathways, biological processes, and organs. These findings were confirmed by compound-target network, target-organ location network, gene ontology analysis, and KEGG pathway enrichment analysis. Most compounds in ATR have been reported to have antifibrillar amyloid plaques, anti-tau phosphorylation, and anti-inflammatory effects. Our results indicated that compounds in ATR interact with multiple targets in a synergetic way. Furthermore, the mRNA expressions of genes targeted by ATR are elevated significantly in heart, brain, and liver. Our results suggest that the anti-inflammatory and immune system enhancing effects of ATR might contribute to its major therapeutic effects on Alzheimer’s disease.

scholarly journals Antidiabetic Properties, Bioactive Constituents, and Other Therapeutic Effects ofScoparia dulcis

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Geethi Pamunuwa ◽  
D. Nedra Karunaratne ◽  
Viduranga Y. Waisundara
Keyword(s):  
Complementary Therapy ◽  
Antimicrobial Activities ◽  
Mechanisms Of Action ◽  
Antidiabetic Activity ◽  
Therapeutic Effects ◽  
Bioactive Constituents ◽  
Scoparia Dulcis ◽  
The Masses ◽  
Alternative And Complementary Therapy

This review discusses the antidiabetic activities ofScoparia dulcisas well as its antioxidant and anti-inflammatory properties in relation to the diabetes and its complications. Ethnomedical applications of the herb have been identified as treatment for jaundice, stomach problems, skin disease, fever, and kidney stones, reproductory issues, and piles. Evidence has been demonstrated through scientific studies as to the antidiabetic effects of crude extracts ofS. dulcisas well as its bioactive constituents. The primary mechanisms of action of antidiabetic activity of the plant and its bioactive constituents are throughα-glucosidase inhibition, curbing of PPAR-γand increased secretion of insulin. Scoparic acid A, scoparic acid D, scutellarein, apigenin, luteolin, coixol, and glutinol are some of the compounds which have been identified as responsible for these mechanisms of action.S. dulcishas also been shown to exhibit analgesic, antimalarial, hepatoprotective, sedative, hypnotic, antiulcer, antisickling, and antimicrobial activities. Given this evidence, it may be concluded thatS. dulciscould be promoted among the masses as an alternative and complementary therapy for diabetes, provided further scientific studies on the toxicological and pharmacological aspects are carried out through eitherin vivoor clinical means.

scholarly journals Proteomic Analysis of Beef Tenderloin and Flank Assessed Using an Isobaric Tag for Relative and Absolute Quantitation (iTRAQ)

Animals ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 150
Author(s):  
Zhaomin Lei ◽  
Jianping Wu ◽  
Deyin Zhang ◽  
Ting Liu ◽  
Shengguo Zhao ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Expression Patterns ◽  
Tca Cycle ◽  
Absolute Quantification ◽  
Absolute Quantitation ◽  
Oxidation Reduction ◽  
The Tca Cycle ◽  
Isobaric Tag ◽  
Isobaric Tags

Herein, we performed a proteomic analysis of tenderloin and flank steaks from Simmental cattle using the isobaric tags for a relative and absolute quantification (iTRAQ) approach. We identified 17 amino acids in both steaks, and Gly, Cys, Ile, Lys, and Pro differed most in abundance between the steak types (p < 0.05). A comparison of the expression patterns in steaks revealed 128 differentially expressed proteins (DEPs), of which 44 were up-regulated and 84 were down-regulated. Furthermore, 27 DEPs (p < 0.05) were subjected to gene ontology (GO) analysis, and many were found to be related to oxidation-reduction, metabolism, hydrogen ion transmembrane transport, transport, the tricarboxylic acid (TCA) cycle, mitochondrial electron transport, and the conversion of nicotinamide adenine dinucleotide (NADH) to ubiquinone. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis also implicated these DEPs in various signalling pathways, including oxidative phosphorylation, cardiac muscle contraction, the TCA cycle, biosynthesis, and the metabolism. These findings provide a new insight into key proteins involved in the determination of amino acid composition in beef.

scholarly journals Identification of novel pathways in pathogenesis of ketosis in dairy cows via iTRAQ/MS

2016 ◽  
Vol 60 (3) ◽  
pp. 309-314 ◽  
Author(s):  
Shi Shu ◽  
Chuchu Xu ◽  
Cheng Xia ◽  
Xinhuan Xiao ◽  
Gang Wang ◽  
...  
Keyword(s):  
Protein Expression ◽  
Dairy Cows ◽  
Expression Profiles ◽  
The Novel ◽  
Absolute Quantitation ◽  
Subclinical Ketosis ◽  
Isobaric Tag ◽  
Production Increase ◽  
Protein Expression Profiles ◽  
New Evidence

AbstractIntroduction: To identify novel pathways involved in the pathogenesis of ketosis, an isobaric tag for relative and absolute quantitation/mass spectrometry was used to define differences in protein expression profiles between healthy dairy cows and those with clinical or subclinical ketosis.Material and Methods: To define the novel pathways of ketosis in cattle, the differences in protein expression were analysed by bioinformatics. Go Ontology and Pathway analysis were carried out for enrich the role and pathway of the different expression proteins between healthy dairy cows and those with clinical or subclinical ketosis.Results: Differences were identified in 19 proteins, 16 of which were relatively up-regulated while the remaining 3 were relatively down-regulated. Sorbitol dehydrogenase (SORD) and glyceraldehyde-3-phosphate dehydrogenase (G3PD) were up-regulated in cattle with ketosis. SORD and G3PD promoted glycolysis. These mechanisms lead to pyruvic acid production increase and ketone body accumulation.Conclusion: The novel pathways of glycolysis provided new evidence for the research of ketosis.

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