scholarly journals The Mechanism of Phillyrin from the Leaves of Forsythia suspensa for Improving Insulin Resistance

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Xiaoqing Xu ◽  
Fatma S. A. Saadeldeen ◽  
Lanting Xu ◽  
Yingying Zhao ◽  
Jinfeng Wei ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Western Blot ◽  
Protein Phosphorylation ◽  
Glucose Uptake ◽  
Glucose Oxidase ◽  
Glucose Consumption ◽  
Significant Activity ◽  
Forsythia Suspensa

Three lignans, phillyrin, forsythia ester A, and rosin-β-D-furan glucose, were isolated from Forsythia suspensa which is a famous Traditional Chinese Medicine used for clearing heat and detoxifying, reducing swelling and dispersing knot, and dispersing wind heat. In this study, the effects of phillyrin, forsythia ester A, and rosin-β-D-furan glucose on insulin resistance of 3T3-L1 adipocytes were investigated by the method of glucose oxidase-peroxidase (GOD-POD) and the mechanism was assayed by the method of western blot. The results indicated that phillyrin, forsythia ester A, and rosin-β-D-furan glucose could improve the glucose uptake in 3T3-L1 adipocytes under insulin resistance (IR). Among them, phillyrin showed significant activity in increasing glucose consumption at the concentrations of 100 μM and 200 μM (P < 0.001). The mechanism of improving insulin resistance may be that phillyrin could raise the protein phosphorylation of IRS-1 and Akt and the expression levels of GLUT4 protein.

scholarly journals Effects of Traditional Chinese Medicine Qinbai Qingfei Concentrated Pellet on Cellular Infectivity of Mycoplasma pneumoniae

2014 ◽  
Vol 2014 ◽  
pp. 1-7
Author(s):  
Guo-Zheng Jiang ◽  
Ting Liu ◽  
Ji-Chang Li
Keyword(s):  
Smooth Muscle ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Airway Smooth Muscle ◽  
Mycoplasma Pneumoniae ◽  
Positive Control ◽  
Control Group ◽  
Significant Activity ◽  
Mtt Method ◽  
Significant Difference

Aim. To study the effect and mechanism of traditional Chinese medicine Qinbai Qingfei concentrated pellet (QQCP) against Mycoplasma pneumoniae (MP). Methods. Rat airway smooth muscle (ASM) cells were used to examine the antimycoplasmal activity of QQCP via four drug-adding modes: pre- and postadding drugs, simultaneous-adding after drug and MP mixed, and simultaneous-adding drug and MP; taking roxithromycin dispersive tablets (RDT) as positive control, the cellular A570 values were determined by MTT method. Results. All of A570 values in QQCP group were significantly higher than those of the corresponding MP control group (P<0.01) in four drug-adding modes; there was no significant difference in A570 values between the QQCP group and that of the positive control group (P>0.05), confirming that QQCP could significantly inhibit the infectivity of MP to ASM cells. Conclusion. QQCP had significant activity in preventing and treating MP infection, killing MP, and antiabsorption.

Characterization of the Active Components of the Multimerized sTNFRII-Adiponectin Fusion Protein Showing Both TNFα-Antagonizing and Glucose Uptake-Promoting Activities

2020 ◽  
Vol 20 (7) ◽  
pp. 1081-1089
Author(s):  
Yao Wang ◽  
Hui Lian ◽  
Xitong Wang ◽  
Tianyu Zheng ◽  
Xiaoxiao Yu ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Fusion Protein ◽  
Glucose Uptake ◽  
Cell Model ◽  
Glucose Consumption ◽  
Antagonistic Activity ◽  
H9c2 Cells ◽  
Active Components ◽  
Exclusion Chromatography

Background: The sTNFRII-adiponectin fusion protein previously showed strong TNFα antagonistic activity. However, the fusion protein exists as mixture of different multimers. The aim of the present study was to characterize its active components. Methods: In this study, the fusion protein was isolated and purified by Ni-NTA affinity and gel exclusion chromatography, and further identified by Coomassie staining and western blotting. The TNFα antagonistic and glucose uptake-promoting activities were determined in vitro. The glucose detection kit and 2- NBDG (2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-glucose) were used to measure their effects on glucose metabolism (including glucose consumption and glucose uptake in HepG2 and H9C2 cells). The effect of the fusion protein on glucose uptake was also examined in free fatty acid (FFA)- induced insulin resistance cell model. Results: The sTNFRII-adiponectin fusion protein was found to exist in three forms: 250 kDa (hexamer), 130 kDa (trimer), and 60 kDa (monomer), with the final purity of 90.2%, 60.1%, and 81.6%, respectively. The fusion protein could effectively antagonize the killing effect of TNFα in L929 cells, and the multimer was found to be superior to the monomer. In addition, the fusion protein could increase glucose consumption without impacting the number of cells (HepG2, H9C2 cells) in a dosedependent manner. Mechanistically, glucose uptake was found to be enhanced by the translocation of GLUT4. However, it could not improve glucose uptake in the cell model of insulin resistance. Conclusion: In summary, the active components of the fusion protein are hexamers and trimers. The hexamer and trimer of sTNFRII-adiponectin fusion protein had both TNFα-antagonizing and glucose uptake-promoting activities, although neither of them could improve glucose uptake in the cell model of insulin resistance.

A diet formula of Puerariae radix, Lycium barbarum, Crataegus pinnatifida, and Polygonati rhizoma alleviates insulin resistance and hepatic steatosis in CD-1 mice and HepG2 cells

2014 ◽  
Vol 5 (5) ◽  
pp. 1038-1049 ◽  
Author(s):  
Jia Liu ◽  
HongJuan Zhang ◽  
BaoPing Ji ◽  
ShengBao Cai ◽  
RuoJun Wang ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Hepatic Steatosis ◽  
Hepg2 Cells ◽  
Multiple Target ◽  
Lycium Barbarum ◽  
Puerariae Radix ◽  
Crataegus Pinnatifida ◽  
Target Organs

According to the principles of traditional Chinese medicine, medicinal and edible herbs exhibit holistic effects through their actions on multiple target organs.

scholarly journals A novel traditional Chinese medicine ameliorates fatigue-induced cardiac hypertrophy and dysfunction via regulation of energy metabolism

2019 ◽  
Vol 316 (6) ◽  
pp. H1378-H1388 ◽  
Author(s):  
Rong Huang ◽  
Yuan-Chen Cui ◽  
Xiao-Hong Wei ◽  
Chun-Shui Pan ◽  
Quan Li ◽  
...  
Keyword(s):  
Body Weight ◽  
Energy Metabolism ◽  
Chinese Medicine ◽  
Food Intake ◽  
Traditional Chinese Medicine ◽  
Cardiac Hypertrophy ◽  
Western Blot ◽  
Prolonged Exercise ◽  
Heart Function ◽  
Heart Weight

Prolonged exercise and exercise training can adversely affect cardiac function in some individuals. QiShenYiQi Pills (QSYQ), which are a compound Chinese medicine, have been previously shown to improve pressure overload-induced cardiac hypertrophy. We hypothesized that QSYQ can ameliorate as well the fatigue-induced cardiac hypertrophy. This study was to test this hypothesis and underlying mechanism with a focus on its role in energy regulation. Male Sprague-Dawley rats were used to establish exercise adaptation and fatigue model on a motorized rodent treadmill. Echocardiographic analysis and heart function test were performed to assess heart systolic function. Food-intake weight/body weight and heart weight/body weight were assessed, and hematoxylin and eosin staining and immunofluorescence staining of myocardium sections were performed. ATP synthase expression and activity and ATP, ADP, and AMP levels were assessed using Western blot and ELISA. Expression of proteins related to energy metabolism and IGF-1R signaling was determined using Western blot. QSYQ attenuated the food-intake weight/body weight decrease, improved myocardial structure and heart function, and restored the expression and distribution of myocardial connexin 43 after fatigue, concomitant with an increased ATP production and a restoration of metabolism-related protein expression. QSYQ upgraded the expression of IGF-1R, P-AMPK/AMPK, peroxisome proliferator-activated receptor-γ coactivator-1α, nuclear respiratory factor-1, P-phosphatidylinositol 3-kinase (PI3K)/PI3K, and P-Akt/Akt thereby attenuated the dysregulation of IGF-1R signaling after fatigue. QSYQ relieved fatigue-induced cardiac hypertrophy and enhanced heart function, which is correlated with its potential to improve energy metabolism by regulating IGF-1R signaling. NEW & NOTEWORTHY Prolonged exercise may impact some people leading to pathological cardiac hypertrophy. This study using an animal model of fatigue-induced cardiac hypertrophy provides evidence showing the potential of QiShenYiQi Pills, a novel traditional Chinese medicine, to prevent the cardiac adaptive hypertrophy from development to pathological hypertrophy and demonstrates that this effect is correlated with its capacity for regulating energy metabolism through interacting with insulin-like growth factor-1 receptor.

scholarly journals Anti-Inflammatory Effects of 81 Chinese Herb Extracts and Their Correlation with the Characteristics of Traditional Chinese Medicine

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Chang-Liang Chen ◽  
Dan-Dan Zhang
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Nitrogen Oxide ◽  
Chinese Herb ◽  
Zingiber Officinale ◽  
Chinese Herbs ◽  
No Production ◽  
Forsythia Suspensa ◽  
Anti Inflammatory ◽  
Daphne Genkwa

Inducible nitrogen oxide synthase (iNOS) is the primary contributor of the overproduction of nitric oxide and its inhibitors have been actively sought as effective anti-inflammatory agents. In this study, we prepared 70% ethanol extracts from 81 Chinese herbs. These extracts were subsequently evaluated for their effect on nitrogen oxide (NO) production and cell growth in LPS/IFNγ-costimulated and unstimulated murine macrophage RAW264.7 cells by Griess reaction and MTT assay. Extracts ofDaphne genkwaSieb.et Zucc,Caesalpinia sappanL.,Iles pubescensHook.et Arn,Forsythia suspensa(Thunb.) Vahl,Zingiber officinaleRosc,Inula japonicaThunb., andLigusticum chuanxiongHort markedly inhibited NO production (inhibition > 90% at 100 μg/mL). Among active extracts (inhibition > 50% at 100 μg/mL),Rubia cordifoliaL.,Glycyrrhiza glabraL.,Iles pubescensHook.et Arn,Nigella glanduliferaFreyn et Sint,Pueraria lobata(Willd.) Ohwi, andScutellaria barbataD. Don displayed no cytotoxicity to unstimulated RAW246.7 cells while increasing the growth of LPS/IFNγ-costimulated cells. By analyzing the correlation between their activities and their Traditional Chinese Medicine (TCM) characteristics, herbs with pungent flavor displayed potent anti-inflammatory capability. Our study provides a series of potential anti-inflammatory herbs and suggests that herbs with pungent flavor are candidates of effective anti-inflammatory agents.

scholarly journals Activation of Insulin Signaling in Adipocytes and Myotubes by Sarcopoterium Spinosum Extract

Nutrients ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 1396 ◽  
Author(s):  
Michaella Ben-Shachar ◽  
Konstantin Rozenberg ◽  
Nir Skalka ◽  
Ayala Wollman ◽  
Michal Michlin ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Medicinal Plant ◽  
Western Blot ◽  
Glucose Uptake ◽  
Insulin Signaling ◽  
Insulin Action ◽  
Maximal Effect ◽  
New Agents ◽  
L6 Myotubes ◽  
Sarcopoterium Spinosum

Sarcopoterium spinosum (S. spinosum) is a medicinal plant, traditionally used as an antidiabetic remedy. Previous studies demonstrated its beneficial properties in the treatment of insulin resistance. The aim of this study was to further clarify the effect of S. spinosum extract (SSE) on insulin signaling. Phosphoproteomic analysis, performed in 3T3-L1 adipocytes treated with SSE, revealed the activation of insulin receptor pathways. SSE increased Glut4-facilitated glucose uptake in adipocytes, with an additive effect between SSE and insulin. While the maximal effect of insulin on glucose uptake was found at days 15–16 of differentiation, SSE-induced glucose uptake was found at an earlier stage of differentiation. Inhibition of PI3K and Akt blocked SSE-dependent glucose uptake. Western blot analysis, performed on 3T3-L1 adipocytes and L6 myotubes, showed that in contrast to insulin action, Akt was only marginally phosphorylated by SSE. Furthermore, GSK3β and PRAS40 phosphorylation as well as glucose uptake were increased by the extract. SSE also induced the phosphorylation of ERK similar to insulin. In conclusion, SSE activates insulin signaling, although the upstream event mediating its effects should be further clarified. Identifying the active molecules in SSE may lead to the development of new agents for the treatment of insulin resistance.

Improving insulin resistance with traditional Chinese medicine in type 2 diabetic patients

Endocrine ◽  
2009 ◽  
Vol 36 (2) ◽  
pp. 268-274 ◽  
Author(s):  
Menglei Chao ◽  
Dajin Zou ◽  
Yifei Zhang ◽  
Yuhong Chen ◽  
Miao Wang ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Type 2 Diabetic

The Effects of Five Alkaloids from Bulbus Fritillariae on the Concentration of cAMP in HEK Cells Transfected with Muscarinic M2 Receptor Plasmid

2006 ◽  
Vol 34 (05) ◽  
pp. 901-910 ◽  
Author(s):  
Ying Zhou ◽  
Hui Ji ◽  
Bao-Qin Lin ◽  
Yan Jiang ◽  
Ping Li
Keyword(s):  
Chinese Medicine ◽  
Calcium Phosphate ◽  
Traditional Chinese Medicine ◽  
Western Blot ◽  
Camp Level ◽  
Rt Pcr ◽  
Camp Concentration ◽  
Hek Cells ◽  
Co Precipitation

The aim of this study was to investigate the effects of five alkaloids, namely verticine, verticinone, imperialine, imperialine-3β-D-glucoside, and puqietinone, purified from Bulbus Fritillariae and used as an antitussive drug in traditional Chinese medicine, on their antimuscarinic M2 function and the cAMP level of HEK cells transfected with muscarinic M2 receptor plasmid. By transfecting the HEK cells with the method of calcium phosphate co-precipitation and screening with G418, the cells stably expressing M2 receptor were identified. The expression of M2 receptor in HEK cells was confirmed by both RT-PCR and western blot. The cAMP level in the treated cells was analyzed with RIA method (125I-cAMP KIT). And the results suggested that the five alkaloids could significantly elevate the cAMP concentration in the HEK cells transfected with muscarinic M2 receptor plasmid ( p < 0.01).

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