scholarly journals Enhancement of the Antibacterial Efficiency of Silver Nanoparticles against Gram-Positive and Gram-Negative Bacteria Using Blue Laser Light

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Anes Al-Sharqi ◽  
Kasing Apun ◽  
Micky Vincent ◽  
Devagi Kanakaraju ◽  
Lesley Maurice Bilung
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Exposure Time ◽  
Cell Membranes ◽  
Laser Light ◽  
Blue Laser ◽  
Resistant Bacteria ◽  
Ag Nps ◽  
Antibacterial Efficiency ◽  
E Coli

Silver nanoparticles (Ag-NPs) possess excellent antibacterial properties and are considered to be an alternative material for treating antibiotic-resistant bacteria. The present study was aimed at enhancing the antibacterial efficiency of Ag-NPs using visible laser light against Escherichia coli and Staphylococcus aureus in vitro. Four concentrations of Ag-NPs (12.5, 25, 50, and 100 μg/ml), synthesized by the chemical reduction method, were utilized to conduct the antibacterial activity of prepared Ag-NPs. The antibacterial efficiencies of photoactivated Ag-NPs against both bacteria were determined by survival assay after exposure to laser irradiation. The mechanism of interactions between Ag-NPs and the bacterial cell membranes was then evaluated via scanning electron microscopy (SEM) and reactive oxygen species analysis to study the cytotoxic action of photoactivated Ag-NPs against both bacterial species. Results showed that the laser-activated Ag-NP treatment reduced the surviving population to 14% of the control in the E. coli population, while the survival in the S. aureus population was reduced to 28% of the control upon 10 min exposure time at the concentration of 50 μg/ml. However, S. aureus showed lower sensitivity after photoactivation compared to E. coli. Moreover, the effects depended on the concentration of Ag-NPs and exposure time to laser light. SEM images of treated bacterial cells indicated that substantial morphological changes occurred in cell membranes after treatment. The results suggested that Ag-NPs in the presence of visible light exhibit strong antibacterial activity which could be used to inactivate harmful and pathogenic microorganisms.

Effective immobilization of silver nanoparticles on a regenerated cellulose–chitosan composite membrane and its antibacterial activity

2017 ◽  
Vol 41 (12) ◽  
pp. 5061-5065 ◽  
Author(s):  
Soon Wei Chook ◽  
Chin Hua Chia ◽  
Sarani Zakaria ◽  
Hui Min Neoh ◽  
Rahman Jamal
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Composite Membrane ◽  
Regenerated Cellulose ◽  
Ag Nps ◽  
E Coli ◽  
Antibacterial Performance ◽  
Cellulose Membranes ◽  
Chitosan Composite

Chitosan–cellulose membranes with high amount of Ag NPs with improved antibacterial performance against S. aureus and E. coli.

scholarly journals Comparison of the antibacterial activity against Escherichia coli of silver nanoparticle produced by chemical synthesis with biosynthesis

2018 ◽  
Vol 2 (2) ◽  
Author(s):  
Nguyen Phuc Quan 1,2 ◽  
Tran Quoc Vinh 1 ◽  
Kieu Thi My Yen 1 ◽  
Le Vu Khanh Trang 2 ◽  
Nguyen Minh Ly 2 ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Color Change ◽  
Chemical Reduction ◽  
Spherical Shape ◽  
Average Diameter ◽  
Ag Nps ◽  
E Coli ◽  
Vis Spectroscopy

The synthesis of silver nanoparticles (Ag NPs) has been carried out using different methods, mainly by biological and chemical methods; however, comparing antibacterial activity of Ag NPs synthesized by these methods has not been conducted before. In this study, silver nanoparticles (Ag NPs) were synthesized by methods using reducing agent NaBH4/carboxymethyl cellulose (CMC) and fungal strain Trichoderma asperellum (T.asperellum). The formation of silver nanoparticles was observed visually by color change and identified by Ultraviolet-visible (UV – vis) spectroscopy. The transmission electron microscopy (TEM) image illustrated almost nanoparticles with spherical shape and average diameter of 4.1 ± 0.2 nm and 2.1 ± 0.2 nm of samples produced from chemical reduction and biosynthesis respectively. Both samples after 180 days storing have been separated lightly, but the agglomeration and absorbance peak shifting were not observed which proved the high stability of synthesized Ag NPs. Antimicrobial activity against human bacterial pathogen Escherichia coli (E. coli) showed that the inhibition zone produced by “biosynthesis” and “chemical reduction” Ag NPs were 3.17 cm and 2.42 cm respectively. With nanoparticles size smaller than 2 mm, antibacterial activity of “biosynthesis” Ag NPs against E. coli was 31 % higher than “chemical reduction” Ag NPs, although the concentration of Ag NPs produced by biosynthesis was about 10-fold less.

scholarly journals Antibacterial activity of royal jelly-mediated green synthesized silver nanoparticles

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Susanna Gevorgyan ◽  
Robin Schubert ◽  
Mkrtich Yeranosyan ◽  
Lilit Gabrielyan ◽  
Armen Trchounian ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Royal Jelly ◽  
Fluorescence Emission ◽  
Spherical Particles ◽  
Ag Nps ◽  
Gram Negative ◽  
Green Synthesized Silver Nanoparticles ◽  
Higher Sensitivity

AbstractThe application of green synthesis in nanotechnology is growing day by day. It’s a safe and eco-friendly alternative to conventional methods. The current research aimed to study raw royal jelly’s potential in the green synthesis of silver nanoparticles and their antibacterial activity. Royal jelly served as a reducing and oxidizing agent in the green synthesis technology of colloidal silver nanoparticles. The UV–Vis maximum absorption at ~ 430 nm and fluorescence emission peaks at ~ 487 nm confirmed the presence of Ag NPs. Morphology and structural properties of Ag NPs and the effect of ultrasound studies revealed: (i) the formation of polydispersed and spherical particles with different sizes; (ii) size reduction and homogeneity increase by ultrasound treatment. Antibacterial activity of different concentrations of green synthesized Ag NPs has been assessed on Gram-negative S. typhimurium and Gram-positive S. aureus, revealing higher sensitivity on Gram-negative bacteria.

scholarly journals Green Synthesized Silver Nanoparticles Immobilized on Activated Carbon Nanoparticles: Antibacterial Activity Enhancement Study and Its Application on Textiles Fabrics

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3790
Author(s):  
Pratama Jujur Wibawa ◽  
Muhammad Nur ◽  
Mukhammad Asy’ari ◽  
Wijanarka Wijanarka ◽  
Heru Susanto ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Activated Carbon ◽  
Aloe Vera ◽  
Particle Size Analysis ◽  
Size Analysis ◽  
E Coli ◽  
Transmission Electron ◽  
Average Size ◽  
Green Synthesized Silver Nanoparticles

This research aimed to enhance the antibacterial activity of silver nanoparticles (AgNPs) synthesized from silver nitrate (AgNO3) using aloe vera extract. It was performed by means of incorporating AgNPs on an activated carbon nanoparticle (ACNPs) under ultrasonic agitation (40 kHz, 2 × 50 watt) for 30 min in an aqueous colloidal medium. The successful AgNPs synthesis was clarified with both Ultraviolet-Visible (UV-Vis) and Fourier Transform Infrared (FTIR) spectrophotometers. The successful AgNPs–ACNPs incorporation and its particle size analysis was performed using Transmission Electron Microscope (TEM). The brown color suspension generation and UV-Vis’s spectra maximum wavelength at around 480 nm confirmed the existence of AgNPs. The particle sizes of the produced AgNPs were about 5 to 10 nm in the majority number, which collectively surrounded the aloe vera extract secondary metabolites formed core-shell like nanostructure of 8.20 ± 2.05 nm in average size, while ACNPs themselves were about 20.10 ± 1.52 nm in average size formed particles cluster, and 48.00 ± 8.37 nm in average size as stacking of other particles. The antibacterial activity of the synthesized AgNPs and AgNPs-immobilized ACNPs was 57.58% and 63.64%, respectively (for E. coli); 61.25%, and 93.49%, respectively (for S. aureus). In addition, when the AgNPs-immobilized ACNPs material was coated on the cotton and polyester fabrics, the antibacterial activity of the materials changed, becoming 19.23% (cotton; E. coli), 31.73% (polyester; E. coli), 13.36% (cotton; S. aureus), 21.15% (polyester; S. aureus).

scholarly journals Synthesis of zinc oxide and silver nanoparticles using ficus palmata - Forssk leaf extracts and assessment of antibacterial activity

2020 ◽  
Vol 26 (6) ◽  
pp. 200454-0
Author(s):  
Sabaoon Shamshad ◽  
Jamshaid Rashid ◽  
Ihsan-ul-haq ◽  
Naseem Iqbal ◽  
Saif Ullah Awan
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Zinc Oxide Nanoparticles ◽  
Inhibitory Concentration ◽  
Oxide Nanoparticles ◽  
Leaf Extracts ◽  
E Coli ◽  
Ficus Palmata

Multidrug resistance of bacteria is an emerging human health hazard and warrants development of novel antibacterial agents with more effective mode of action. Here, zinc oxide and silver nanomaterials were prepared using Ficus palmata Forssk leaf extract with efficient antibacterial activity. SEM coupled with EDS confirmed the spherical symmetry with average particle diameter 50 to 65 nm while the XRD confirmed crystalline face centered cubic structure of silver and hexagonal crystallize phase of zinc oxide nanoparticles. Antibacterial activity was evaluated for 8 pathogenic bacterial strains including 3 drug resistant pathogenic strains. The nanoparticles showed enhanced growth inhibition for resistant strains in comparison with the broad-spectrum antibiotics i.e. roxithromycin and cefixime. Minimum inhibitory concentration in μg.mL<sup>-1</sup> of silver nanoparticles was found to be as low as 33.3 for resistant Streptococcus haemolyticus; 11.1 for Staphylococcus aureus and E Coli; and 3.7 μg.mL<sup>-1</sup> for resistant Pseudomonas aeruginosa. Similarly, the minimum inhibitory concentration of zinc oxide nanoparticles was found to be 100 μg.mL<sup>-1</sup> against resistant Streptococcus haemolyticus and Staphylococcus aureus; 11.1 μg.mL<sup>-1</sup> for resistant Pseudomonas aeruginosa; and 3.7 μg.mL<sup>-1</sup> against resistant E coli. Ficus palmata Forssk leaf extracts can be explored effectively for synthesizing active antibacterial nanomaterials as a non-toxic and environmentally benign synthesis route.

scholarly journals Green ultrasonic synthesis, Characterization and Antibacterial activity of Silver and Gold Nanoparticles mediated by Ganoderma lucidum extract

2020 ◽  
Vol 4 (2) ◽  
Keyword(s):  
Gold Nanoparticles ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Metal Nanoparticles ◽  
Cytotoxic Activities ◽  
Gold And Silver Nanoparticles ◽  
Silver And Gold Nanoparticles ◽  
E Coli ◽  
Inhibition Zones

Metal nanoparticles possess an extensive scientific and technological significance due to their unique physiochemical properties and their potential applications in different fields like medicine. Silver and gold nanoparticles have shown to have antibacterial and cytotoxic activities. Conventional methods used in the synthesis of the metal nanoparticles involve use of toxic chemicals making them unsuitable for use in medical field. In our continued effort to explore for simple and eco-friendly methods to synthesize the metal nanoparticles, we here describe synthesis and characterization of gold and silver nanoparticles using Gonaderma lucidum, wild non-edible medicinal mushroom. G. lucidum mushroom contain bioactive compounds which can be involved in the reduction, capping and stabilization of the nanoparticles. Antibacterial activity analysis was done on E. coli and S. aureus. The synthesis was done on ultrasonic bath. Characterization of the metal nanoparticles was done by UV-VIS., High Resolution Transmission Electron Microscope (HRTEM) and FTIR. HRTEM analysis showed that both silver and gold nanoparticles were spherical in shape with an average size of 15.82±3.69 nm for silver and 24.73±5.124nm for gold nanoparticles (AuNPs). FTIR analysis showed OH and -C=C- stretching vibrations, an indication of presence of functional groups of biomolecules capping both gold and silver nanoparticles. AgNPs showed inhibition zones of 15.5±0.09mm and 13.3±0.14mm while AuNPs had inhibition zones of 14.510±0.35 and 13.3±0.50mm on E. coli and S. aureus respectively. The findings indicate the potential use of AgNPs and AuNPs in development of drugs in management of pathogenic bacteria.

Protein-stabilized silver nanoparticles encapsulating gentamycin for the therapy of bacterial biofilm infections

Nanomedicine ◽  
2021 ◽  
Author(s):  
Sudip Nag ◽  
Arpita Biswas ◽  
Dhrubajyoti Chattopadhyay ◽  
Maitree Bhattacharyya
Keyword(s):  
Silver Nanoparticles ◽  
Bacterial Infections ◽  
Microscopic Analysis ◽  
Bacterial Biofilm ◽  
Resistant Bacteria ◽  
Hydrodynamic Diameter ◽  
Drug Resistant ◽  
Electron Microscopic ◽  
Antibacterial Efficiency ◽  
Control Drug

Aim: An antibiotic-conjugated protein-stabilized nanoparticle hybrid system was developed to combat the challenges faced during the treatment of drug-resistant bacterial biofilm-associated infections. Materials & methods: Biocompatible silver nanoparticles were synthesized using intracellular protein and gentamycin was attached. The resulting nanohybrid was characterized and its antibacterial efficiency was assessed against Gram-positive, Gram-negative and drug-resistant bacteria. Results: Spectroscopic and electron microscopic analysis revealed that the nanoparticles were spherical with a diameter of 2–6 nm. Red-shifting of the surface plasmon peak and an increase in hydrodynamic diameter confirmed attachment of gentamycin. The nanohybrid exhibited antibacterial efficiency against a range of bacteria with the ability to inhibit and disrupt bacterial biofilm. Conclusion: A unique nanohybrid was designed that has potential to be used to control drug-resistant bacterial infections in the future.

scholarly journals A Surface Dielectric Barrier Discharge Plasma for Preparing Cotton-Fabric-Supported Silver Nanoparticles

Nanomaterials ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 961 ◽  
Author(s):  
Zhiyuan Fan ◽  
Lanbo Di ◽  
Xiuling Zhang ◽  
Hongyang Wang
Keyword(s):  
Silver Nanoparticles ◽  
Dielectric Barrier Discharge ◽  
Cotton Fabric ◽  
Barrier Discharge ◽  
Dbd Plasma ◽  
Ag Nps ◽  
Dielectric Barrier ◽  
E Coli ◽  
Plasma Method ◽  
Surface Dielectric Barrier Discharge

Cotton-fabric-supported silver nanoparticles (Ag NPs) have aroused great attention due to their remarkable physical and chemical properties and excellent broad-spectrum antibacterial performance.In this work, a surface dielectric barrier discharge (DBD) plasma method is developed and employed to prepare cotton fabric supported Ag NPs (Ag/cotton) for the first time. UV-Vis and X-ray photoelectron spectroscopy (XPS) results confirm the formation of Ag NPs. TEM images show that the size of Ag NPs is in the range 4.8–5.3 nm. Heat-sensitive cotton fabrics are not destroyed by surface DBD plasma according to FTIR and XRDresults. Wash fastness of the Ag/cotton samples is investigated using ultrasonic treatment for 30 min and it is shown that the Ag NPs possess good adhesion to the cotton fabric according to UV-Vis spectra. Antibacterial activity of the Ag/cotton samples shows that obvious bacteriostasis loops are observed around the samples with the appearance of both Gram-negative bacterium Escherichia coli (E. coli) and Gram-positive bacterium Bacillus subtilis (B. subtilis). The average diameter of the bacteriostasis loops against both E. coli and B. subtilis becomes larger with an increasing silver loading amount.This work provides a universal, fast, simple, and environmentally-friendly cold plasma method for synthesizing Ag NPs on heat-sensitive materials at atmospheric pressure.

scholarly journals Synthesis and biological characterization of silver nanoparticles derived from the cyanobacterium Oscillatoria limnetica

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Ragaa A. Hamouda ◽  
Mervat H. Hussein ◽  
Rasha A. Abo-elmagd ◽  
Salwa S. Bawazir
Keyword(s):  
Silver Nanoparticles ◽  
Cell Line ◽  
Fourier Transforms ◽  
Human Colon ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Ag Nps ◽  
Human Colon Cancer ◽  
Visible Absorption ◽  
Ft Ir

Abstract Using aqueous cyanobacterial extracts in the synthesis of silver nanoparticle is looked as green, ecofriendly, low priced biotechnology that gives advancement over both chemical and physical methods. In the current study, an aqueous extract of Oscillatoria limnetica fresh biomass was used for the green synthesis of Ag-NPs, since O. limnetica extract plays a dual part in both reducing and stabilizing Oscillatoria-silver nanoparticles (O-AgNPs). The UV-Visible absorption spectrum, Fourier transforms infrared (FT-IR), transmission electron microscopy (TEM) and scanning electron microscope (SEM) were achieved for confirming and characterizing the biosynthesized O-AgNPs. TEM images detected the quasi-spherical Ag-NPs shape with diverse size ranged within 3.30–17.97 nm. FT-IR analysis demonstrated the presence of free amino groups in addition to sulfur containing amino acid derivatives acting as stabilizing agents as well as the presence of either sulfur or phosphorus functional groups which possibly attaches silver. In this study, synthesized Ag-NPs exhibited strong antibacterial activity against multidrug-resistant bacteria (Escherichia coli and Bacillus cereus) as well as cytotoxic effects against both human breast (MCF-7) cell line giving IC50 (6.147 µg/ml) and human colon cancer (HCT-116) cell line giving IC50 (5.369 µg/ml). Hemolytic activity of Ag-NPs was investigated and confirmed as being non- toxic to human RBCs in low concentrations.

scholarly journals Silver Nanoparticles: Biosynthesis Using an ATCC Reference Strain ofPseudomonas aeruginosaand Activity as Broad Spectrum Clinical Antibacterial Agents

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Melisa A. Quinteros ◽  
Ivana M. Aiassa Martínez ◽  
Pablo R. Dalmasso ◽  
Paulina L. Páez
Keyword(s):  
Silver Nanoparticles ◽  
Broad Spectrum ◽  
Nitrate Solution ◽  
Human Neutrophils ◽  
Resistant Bacteria ◽  
Klebsiella Pneumonia ◽  
Bacterial Strains ◽  
Ag Nps ◽  
Tem Analysis ◽  
Vis Spectroscopy

Currently, the biosynthesis of silver-based nanomaterials attracts enormous attention owing to the documented antimicrobial properties of these ones. This study reports the extracellular biosynthesis of silver nanoparticles (Ag-NPs) using aPseudomonas aeruginosastrain from a reference culture collection. A greenish culture supernatant ofP. aeruginosaincubated at 37°C with a silver nitrate solution for 24 h changed to a yellowish brown color, indicating the formation of Ag-NPs, which was confirmed by UV-vis spectroscopy, transmission electron microscopy, and X-ray diffraction. TEM analysis showed spherical and pseudospherical nanoparticles with a distributed size mainly between 25 and 45 nm, and the XRD pattern revealed the crystalline nature of Ag-NPs. Also it provides an evaluation of the antimicrobial activity of the biosynthesized Ag-NPs against human pathogenic and opportunistic microorganisms, namely,Staphylococcus aureus,Staphylococcus epidermidis,Enterococcus faecalis,Proteus mirabilis,Acinetobacter baumannii,Escherichia coli,P. aeruginosa, andKlebsiella pneumonia. Ag-NPs were found to be bioactive at picomolar concentration levels showing bactericidal effects against both Gram-positive and Gram-negative bacterial strains. This work demonstrates the first helpful use of biosynthesized Ag-NPs as broad spectrum bactericidal agents for clinical strains of pathogenic multidrug-resistant bacteria such as methicillin-resistantS. aureus,A. baumannii, andE. coli. In addition, these Ag-NPs showed negligible cytotoxic effect in human neutrophils suggesting low toxicity to the host.

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