scholarly journals CEMP-1 Levels in Periodontal Wound Fluid during the Early Phase of Healing: Prospective Clinical Trial

2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
Claudia Dellavia ◽  
Elena Canciani ◽  
Giulio Rasperini ◽  
Giorgio Pagni ◽  
Matteo Malvezzi ◽  
...  
Keyword(s):  
Surgical Procedures ◽  
Early Phase ◽  
Gingival Crevicular Fluid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Wound Fluid ◽  
Protein Levels ◽  
Inflammatory Phase ◽  
Crevicular Fluid ◽  
Open Flap Debridement ◽  
Periodontal Healing

Objectives. Cementogenesis seems to be significantly compromised during tissue inflammation. In dental practice, surgical procedures are performed with the aim to regenerate periodontium including cementum. However, inflammation that occurs during the initial healing phases after surgery may impair regeneration of this tissues. The aim of the present study was to assess if surgical procedures designed to regenerate periodontium might affect levels of cementum protein-1 (CEMP-1) in periodontal wound fluid during early phase of healing. Materials and Methods. In 36 patients, 18 intrabony periodontal defects were treated with regenerative therapy (REG group) and 18 suprabony periodontal defects were treated with open flap debridement (OFD group). In the experimental sites, gingival crevicular fluid was collected immediately before surgery, and periodontal wound fluid was collected 4, 7, 14, and 21 days after surgery. CEMP-1 levels were detected by indirect enzyme-linked immunosorbent assay technique. Results. At the analysis, it resulted that there was a significant average difference in CEMP-1 values between the REG and OFD groups at baseline (p=0.041), the CEMP-1-modeled average in the OFD group was lower by 0.45 ng/ml. There was a significant trend in CEMP-1 over time, and this trend was different among the 2 groups: the REG group showed a statistically significant rising CEMP-1 trend (0.18 ng/ml a week p=0.012), while the OFD had a trend that was significantly lower (-0.22 ng/ml a week compared to the REG group trend p=0.023), the OFD group lost on average 0.05 ng/ml a week. In REG sites, GCF protein levels resulted also related to clinical parameters. Conclusions. During the initial inflammatory phase of periodontal healing, CEMP-1 levels decrease regardless of the surgical protocol applied. The surgical procedures used to regenerate periodontal tissue are able to reverse this trend and to induce significant increase of CEMP-1 in periodontal wound fluid after the first week postop.

scholarly journals Vitamin D-Binding Protein Levels in Plasma and Gingival Crevicular Fluid of Patients with Generalized Aggressive Periodontitis

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Xin Zhang ◽  
Huanxin Meng ◽  
Li Xu ◽  
Li Zhang ◽  
Dong Shi ◽  
...  
Keyword(s):  
Vitamin D ◽  
Gingival Crevicular Fluid ◽  
Smoking Status ◽  
Binding Protein ◽  
Aggressive Periodontitis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Controls ◽  
Vitamin D Binding Protein ◽  
Protein Levels ◽  
Crevicular Fluid

Vitamin D-binding protein (DBP) is the main transport protein of vitamin D and plays an important role in the immune system and host defenses. The purpose of this study was to measure DBP levels in plasma and gingival crevicular fluid (GCF) of patients with generalized aggressive periodontitis (GAgP), in comparison to healthy controls, with the goal of elucidating the relationship between DBP and GAgP. Fifty-nine GAgP patients and 58 healthy controls were recruited for the study; clinical parameters of probing depths (PD), bleeding index, and attachment loss (AL) were recorded. DBP levels were measured by enzyme-linked immunosorbent assay. From the results, GAgP patients had higher plasma DBP concentrations (P<0.001) but lower GCF DBP concentrations (P<0.001) than healthy controls. In GAgP group, after controlling the potential confounders of age, gender, smoking status, and BMI index, GCF DBP concentrations correlated negatively with PD (P<0.001) and AL (P=0.009). Within the limits of the study, we concluded that decreased GCF DBP level and increased plasma DBP level are associated with periodontitis.

scholarly journals Effects of Initial Periodontal Therapy on Heat Shock Protein 70 Levels in Gingival Crevicular Fluid from Periodontitis Patients

2020 ◽  
Vol 9 (10) ◽  
pp. 3072
Author(s):  
Nobuhisa Furuse ◽  
Hideki Takai ◽  
Yorimasa Ogata
Keyword(s):  
Heat Shock ◽  
Gingival Crevicular Fluid ◽  
Periodontal Therapy ◽  
Enzyme Linked Immunosorbent Assay ◽  
Microbial Infection ◽  
Pocket Depth ◽  
Probing Pocket Depth ◽  
Factor Α ◽  
Crevicular Fluid ◽  
Shock Proteins

Periodontitis is an inflammatory disease of periodontium which is caused by periodontopathic bacteria. Moreover, various cytokines such as interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and IL-6 are expressed in the inflamed periodontium. Heat shock proteins (HSPs) protect cells from abnormal conditions including inflammation, microbial infection and diseases. The 70-kDa HSPs (HSP70s) are major HSPs that express in the inflamed tissues. In this study, an enzyme-linked immunosorbent assay was applied to measure the levels of HSP70 in gingival crevicular fluid (GCF) from two periodontal pockets in each of 10 patients with Stage III, Grade B periodontitis. Sites with probing pocket depth (PPD) of ≤3 mm were named the healthy control (HC) sites, and sites with PPD of ≥5 mm were named the diseased sites. HSP70 levels in GCF were expressed higher at diseased sites than at HC sites, and decreased after initial periodontal therapy at diseased sites. These results suggest the association of HSP70 with the stage of periodontitis.

scholarly journals Salivary Levels of IL-6 and IL-17 Could Be an Indicator of Disease Severity in Patients with Calculus Associated Chronic Periodontitis

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Husniah Batool ◽  
Ahmed Nadeem ◽  
Muhammad Kashif ◽  
Faheem Shahzad ◽  
Romeeza Tahir ◽  
...  
Keyword(s):  
Chronic Periodontitis ◽  
Alveolar Bone ◽  
Gingival Crevicular Fluid ◽  
Severe Disease ◽  
Cross Sectional Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Controls ◽  
Cross Sectional ◽  
Significant Difference ◽  
Crevicular Fluid

Background/Purpose. Chronic periodontitis is an inflammatory disease of gums that causes loss of supporting structures of teeth, that is, gingiva, periodontal ligament, cementum, and alveolar bone. Levels of various cytokines in the serum, gingival tissues, and gingival crevicular fluid in patients with chronic periodontitis have been studied, but limited data are available on the level of cytokines in saliva. Therefore, a study was designed to determine levels of salivary IL-6 and IL-17 in patients with calculus associated chronic periodontitis. Materials and Methods. It was a comparative, cross-sectional study that is comprised of 41 healthy controls and 41 calculus associated chronic periodontitis patients (CP patients). According to the degree of attachment loss, CP patients were subcategorized as mild (CAL 1-2 mm), moderate (CAL 3-4 mm), and severe (CAL > 5 mm) forms of periodontitis. Salivary levels of IL-6 and IL-17 were determined using enzyme-linked immunosorbent assay (ELISA) technique. Data was analyzed using SPSS 20.0. Results. Between healthy controls and CP patients (moderate and severe disease), a statistically significant difference was observed in the concentrations of IL-6 and IL-17. In CP patients, the highest mean ± SD of salivary IL-6 and IL-17 was observed in severe CP, followed by moderate and mild CP. Regarding level of IL-6, a statistically significant difference was observed between mild and severe disease and between moderate and severe subcategories of CP patients. Similarly, statistically significant difference was observed in the level of IL-17 between mild and moderate, mild and severe disease, and moderate and severe disease. Conclusion. The levels of salivary IL-6 and IL-17 were increased significantly in calculus associated CP patients as compared to healthy controls and these levels increased with the progression of CP. Clinical Significance. Salivary levels of IL-6 and IL-17 may help in the subcategorization of CP.

scholarly journals Gingival Crevicular Fluid Calprotectin, Osteocalcin and Cross-Linked N-Terminal Telopeptid Levels in Health and Different Periodontal Diseases

2011 ◽  
Vol 31 (6) ◽  
pp. 343-352 ◽  
Author(s):  
Sema Becerik ◽  
Beral Afacan ◽  
Veli Özgen Öztürk ◽  
Harika Atmaca ◽  
Gülnur Emingil
Keyword(s):  
Healthy Subjects ◽  
Gingival Crevicular Fluid ◽  
Periodontal Diseases ◽  
Inflammatory Marker ◽  
Aggressive Periodontitis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Probing Depth ◽  
Crevicular Fluid ◽  
First Time

Aim:The aim of the present study was to investigate gingival crevicular fluid (GCF) calprotectin, osteocalcin and cross-linked N-terminal telopeptide (NTx) levels in health along with different periodontal diseases.Material and methods:Twenty chronic periodontitis (CP), 20 generalized aggressive periodontitis (G-AgP), 20 gingivitis and 20 healthy subjects were included. Probing depth, clinical attachment level, plaque index and papillary bleeding index was recorded. GCF calprotectin, osteocalcin and NTx levels were analyzed by enzyme-linked immunosorbent assay (ELISA).Results:CP, G-AgP and gingivitis groups had higher GCF calprotectin total amount compared to healthy subjects (p< 0.008). CP and G-AgP groups had similar, but higher levels compared to gingivitis groups (p< 0.008). CP and G-AgP groups had lower GCF osteocalcin total amount compared to gingivitis and healthy groups (p< 0.008). CP group had higher GCF NTx but lower osteocalcin total amount and osteocalcin/NTx ratio than the G-AgP group (p< 0.008)Conclusions:Our results suggest that elevated GCF calprotectin levels play a role as a reliable inflammatory marker in the pathogenesis of periodontal disease. Fluctuating GCF levels of osteocalcin and NTx might point out to the abnormal bone turnover in periodontitis. Our data document for the first time the role of NTx in the pathogenesis of different periodontal diseases.

scholarly journals S100A8 and S100A9 in saliva, blood and gingival crevicular fluid for screening periodontitis: a cross-sectional study

2020 ◽  
Author(s):  
Hyun-Duck Kim ◽  
Sandeep Karna ◽  
YooJin Shin ◽  
Huong Vu ◽  
Hyun-Jae Cho ◽  
...  
Keyword(s):  
Gingival Crevicular Fluid ◽  
Characteristic Curve ◽  
Analysis Of Covariance ◽  
Enzyme Linked Immunosorbent Assay ◽  
Oral Diseases ◽  
Early Screening ◽  
Clinical Attachment Loss ◽  
Cross Sectional ◽  
Crevicular Fluid ◽  
Healthy Participants

Abstract Background: Periodontitis is one of major oral diseases, which has no consensus on early screening tool. This study aimed to compare the association and screening ability of S100A8 and S100A9 in saliva, blood and gingival crevicular fluid (GCF) for periodontitis.Methods: We recruited 149 community Koreans, 50 healthy and 99 periodontitis. Using clinical attachment loss and a panoramic radiograph, stage II-IV of new classification of periodontitis proposed at 2018 was considered as periodontitis. Enzyme linked immunosorbent assay kit was used to quantify S100A8 and S100A9. T-test, analysis of covariance (ANCOVA), Mann-Whitney test and correlation analysis were applied to compare the relationship of S100A8 and S100A9 in saliva, blood, and GCF for periodontitis. Receiver operating characteristic curve was applied for screening ability.Results: Among S100A8 and S100A9 in saliva, blood and GCF, S100A8 in saliva was significantly higher in periodontitis participants than in healthy participants (p<0.05) and showed highest screening ability of 0.73 for periodontitis. However, S100A8 and S100A9 in GCF were significantly higher in healthy participants (p<0.05). Salivary S100A8 was positively correlated to blood S100A8 (r=0.21, p <0.05).Conclusion: Salivary S100A8 could be a potential diagnostic marker for periodontitis. Thus, S100A8 salivary kit will be useful for screening periodontitis.

scholarly journals Pentraxin-3 Levels in Gingival Crevicular Fluid during Canine Retraction with Nickel–Titanium Coil Spring and Active Tieback

2017 ◽  
Vol 18 (8) ◽  
pp. 710-713
Author(s):  
Sukhpreet Mangat ◽  
Rakesh Thukral ◽  
Anvesha Ganguly ◽  
Harleen Bali ◽  
Shekhar Grover
Keyword(s):  
Orthodontic Treatment ◽  
Gingival Crevicular Fluid ◽  
Nickel Titanium ◽  
Enzyme Linked Immunosorbent Assay ◽  
Pentraxin 3 ◽  
Coil Spring ◽  
Time Intervals ◽  
Canine Retraction ◽  
Significant Difference ◽  
Crevicular Fluid

ABSTRACT Aim Orthodontic treatment is routinely carried out in patients with the purpose of correcting various forms of dental malocclusions. Retraction of the canines can be achieved either individually or along with incisor. Pentraxin-3 (PTX-3) is regarded as the true independent indicator of disease activity. Hence, we undertook the present study to assess and compare the level of PTX-3 in patients undergoing canine retraction with active tieback and Nickel–Titanium (NiTi) coil spring. Materials and methods The present study included assessment of 25 patients that underwent canine retraction as a part of fixed orthodontic treatment. In the maxillary right and left quadrant, active tieback and NiTi coil spring were used respectively. Gingival crevicular fluid samples were collected 1 hour prior to the commencement of orthodontic canine retraction procedure followed by collection at following time intervals: 1 hour, 1, 7, and 14 days after the start of canine retraction procedure. Enzyme-linked immunosorbent assay kit was used for analysis of the samples as per manufacturer's instructions. All the data were recorded and compiled. All the results were analyzed by Statistical Package for the Social Sciences software. Results Nonsignificant difference was observed in the values of PTX-3 at baseline and 1 hour. While comparing the mean PTX-3 values between different time intervals in both the study groups, significant difference was observed. Conclusion In comparison to the active tieback, NiTi coil exhibited faster space closure rate. Clinical significance In patients undergoing orthodontic treatment, PTX-3 is associated with periodontal remodeling under the effect of orthodontic forces. How to cite this article Thukral R, Mangat S, Ganguly A, Agarkar SS, Bali H, Grover S. Pentraxin-3 Levels in Gingival Crevicular Fluid during Canine Retraction with Nickel–Titanium Coil Spring and Active Tieback. J Contemp Dent Pract 2017; 18(8):710-713.

scholarly journals GCF Adrenomedullin Levels in Healthy and Periodontitis Patients with or without Type 2—Diabetes Mellitus: Clinicobiochemical Study

2014 ◽  
Vol 5 (1) ◽  
pp. 42-46
Author(s):  
SM Apoorva ◽  
Divya Bhat ◽  
Akanksha Garg ◽  
A Suchetha ◽  
N Sapna ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Gingival Crevicular Fluid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Pocket Depth ◽  
Probing Pocket Depth ◽  
Crevicular Fluid

ABSTRACT Background The literature suggests that periodontal disease and diabetes mellitus share a two-way relationship. The aim of this study was to evaluate and compare the levels of adrenomedullin (ADM) in gingival crevicular fluid (GCF) of periodontally healthy and periodontitis patients with or without type 2 diabetes with different glycemic controls. Methods Ninety patients were included in the study and were divided into five groups based on CPI scores and ADA classification of diabetes. Probing pocket depth (PPD) and clinical attachment level (CAL) were measured in all the subjects. GCF was collected from all the participants using micropipettes. ADM levels were measured in GCF samples by enzyme-linked immunosorbent assay. Results The results showed higher levels of ADM in patients with periodontitis as compared to healthy group. Significant correlation was present between PPD and CAL and ADM levels in all periodontitis patients with or without type 2 diabetes. Conclusion Increase in GCF levels of ADM from periodontal health to disease and in periodontitis patients with type 2 diabetes with the worsening of glycemic control underlines the possible role of ADM in mounting a protective response to worsening disease state. How to cite this article Garg A, Suchetha A, Sapna N, Apoorva SM, Bhat D, Puzhankara L. GCF Adrenomedullin Levels in Healthy and Periodontitis Patients with or without Type 2—Diabetes Mellitus: Clinicobiochemical Study. World J Dent 2014;5(1):42-46.

scholarly journals An Evaluation and Correlation of Leptin in Gingival Crevicular Fluid and Serum in Health, Gingivitis and Periodontitis

2012 ◽  
Vol 1 (2) ◽  
pp. 93-97
Author(s):  
Vinay Vadvadgi ◽  
Neeta Padmawar
Keyword(s):  
Periodontal Disease ◽  
Gingival Crevicular Fluid ◽  
Gingival Tissue ◽  
Enzyme Linked Immunosorbent Assay ◽  
Group Iii ◽  
Group I ◽  
The Mean ◽  
Group Ii ◽  
Crevicular Fluid ◽  
Mean Concentration

ABSTRACT Background and objective Plasma leptin is associated in patients with inflammatory diseases. A high concentration of leptin is associated with healthy gingival tissue. The purpose of this study was to assess the concentration of human leptin in gingival crevicular fluid (GCF) and serum within healthy and diseased gingiva, further to explore the possibility of using the levels of leptin in GCF and serum as a biochemical marker of periodontal disease progression. Materials and methods Ninety subjects were selected with age (30-39 years) and sex (15 males and 15 females) matched, to eliminate age and sex as confounders. The subjects were divided into three groups consisting of 30 subjects in each group based on the clinical and radiological parameters; healthy (group I), gingivitis (group II), periodontitis (group III), from whom the GCF samples were collected with Periopaper GCF collection strips (Proflow, Amityville, NY, USA) for 30 seconds and blood samples with 20-gauge needle syringe respectively. Leptin concentration was determined from individual GCF and serum samples by enzyme-linked immunosorbent assay (ELISA). Results The highest mean leptin concentration in GCF was observed in group I (2,664.30 pg/ml ± 324.73) and least mean leptin concentration was obtained in group III (1,309.43 pg/ml ± 202.45). The mean concentration of group II (1,639.43 pg/ml ± 344.46) was intermediate between the highest and lowest values. In contrast, the highest mean leptin concentration in serum was obtained for group III (12,086.57 pg/ml ± 1,698.23) and least mean leptin concentration was obtained for group I (8,715.09 pg/ml ± 1,649.19). The mean concentration of the group II (10,694.01 pg/ml ± 1,777.72) were intermediate between the highest and lowest values. Conclusion The results indicated a statistically significant decrease in the GCF leptin concentration and increase in serum leptin concentration as the periodontal disease progressed. How to cite this article Vadvadgi VH, Saini R, Padmawar N. An Evaluation and Correlation of Leptin in Gingival Crevicular Fluid and Serum in Health, Gingivitis and Periodontitis. Int J Experiment Dent Sci 2012;1(2):93-97.

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