scholarly journals Biogenic Synthesis of Copper and Silver Nanoparticles Using Green Alga Botryococcus braunii and Its Antimicrobial Activity

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Anju Arya ◽  
Khushbu Gupta ◽  
Tejpal Singh Chundawat ◽  
Dipti Vaya
Keyword(s):  
Silver Nanoparticles ◽  
Green Alga ◽  
Botryococcus Braunii ◽  
Biologically Active ◽  
Bacterial Strains ◽  
X Ray Diffraction ◽  
X Ray ◽  
Synthesis Of Nanoparticles ◽  
Vis Spectroscopy ◽  
Active Nanoparticles

The spread of infectious diseases and the increase in the drug resistance among microbes has forced the researchers to synthesize biologically active nanoparticles. Improvement of the ecofriendly procedure for the synthesis of nanoparticles is growing day-by-day in the field of nanobiotechnology. In the present study, we use the extract of green alga Botryococcus braunii for the synthesis of copper and silver nanoparticles. The characterization of copper and silver nanoparticles was carried out by using UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron spectroscopy (SEM). FTIR measurements showed all functional groups having control over reduction and stabilization of the nanoparticles. The X-ray diffraction pattern revealed that the particles were crystalline in nature with a face-centred cubic (FCC) geometry. SEM micrographs have shown the morphology of biogenically synthesized metal nanoparticles. Furthermore, these biosynthesized nanoparticles were found to be highly toxic against two Gram-negative bacterial strains Pseudomonas aeruginosa (MTCC 441) and Escherichia coli (MTCC 442), two Gram-positive bacterial strains Klebsiella pneumoniae (MTCC 109) and Staphylococcus aureus (MTCC 96), and a fungal strain Fusarium oxysporum (MTCC 2087). The zone of inhibition was measured by the agar well plate method, and furthermore, minimum inhibitory concentration was determined by the broth dilution assay.

scholarly journals Biophysical and Characterizations of Silver Nanoparticles used as Salmonella typhi Detector

2014 ◽  
Vol 2 (4) ◽  
pp. 510-515
Author(s):  
Hala Moustafa Ahmed
Keyword(s):  
Silver Nanoparticles ◽  
Salmonella Typhi ◽  
Bacterial Strains ◽  
X Ray Diffraction ◽  
X Ray ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Combined Action ◽  
Hog Plum ◽  
Transmission Electronmicroscopy

The present study mainly focuses of combined action of Nepali hog plum as well as citrate synthesized silver nanoparticles (AgNPs) and Amikacin, as an antibiotic. The synergistic actions of citrate stabilized silver nanoparticles (AgNPs with chem) were compared with that of Nepali hog plum Choerospondia saxillaris (Lapsi) synthesized silver nanoparticles (AgNPs with plant), together with action of antibiotic onselected bacterial strains of Salmonella typhi. The synthesized AgNPs were characterized through UV-Vis spectroscopy, Transmission electronmicroscopy and X-ray diffraction technique. The size of the synthesized silver nanoparticles was measured by Transmission Electron Microscope (TEM) and X-ray diffraction (XRD).DOI: http://dx.doi.org/10.3126/ijasbt.v2i4.11127 Int J Appl Sci Biotechnol, Vol. 2(4): 510-515 

scholarly journals Green synthesis of Ag/TiO2 Nanocomposite Assisted by Gambier Leaf (Uncaria gambir Roxb) Extract

2019 ◽  
Vol 22 (6) ◽  
pp. 250-255 ◽  
Author(s):  
Sry Wahyuni ◽  
Syukri Syukri ◽  
Syukri Arief
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Diffuse Reflectance Spectroscopy ◽  
Irradiation Time ◽  
Rutile Phase ◽  
X Ray Diffraction ◽  
Silver Surface ◽  
X Ray ◽  
Synthesis Of Nanoparticles ◽  
Vis Spectroscopy

Studies of green synthesis of nanoparticles mediated by plants extract is extensively explored and studied in recent time due to eco-friendliness, cost-effectiveness, and use a minimum amount of toxic chemicals in the process of inorganic material synthesis. In this study, the immobilization of silver nanoparticles on the surface of titanium dioxide (TiO2) was carried out using Uncaria gambier Roxb. leaf extract as a silver ion (Ag+) reducing agent. The synthesized Ag/TiO2 nanocomposite was characterized by UV-Vis spectroscopy, X-ray diffraction (XRD), and Diffuse Reflectance Spectroscopy (DRS). The formation of silver nanoparticles was confirmed through UV-Vis spectroscopic analysis, which showed a silver surface plasmon resonance (SPR) band at 426 nm. The X-ray diffraction pattern shows that Ag can inhibit the transition of the anatase into rutile phase. The presence of Ag particles in TiO2 can increase the absorption ability from an initial wavelength of 407 nm to 438 nm. Based on the results of Rhodamin B degradation, it can be seen that Ag/TiO2 has a higher photocatalytic activity than bare TiO2 with 99% percent degradation at 120 minutes of irradiation time.

scholarly journals Biosynthesis, Characterization, and Antidermatophytic Activity of Silver Nanoparticles Using Raamphal Plant (Annona reticulata) Aqueous Leaves Extract

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
P. Shivakumar Singh ◽  
G. M. Vidyasagar
Keyword(s):  
Silver Nanoparticles ◽  
Chemical Compounds ◽  
Visual Observation ◽  
X Ray Diffraction ◽  
Tem Analysis ◽  
X Ray ◽  
Leaf Aqueous Extract ◽  
Vis Spectroscopy ◽  
Antidermatophytic Activity ◽  
Annona Reticulata

The present work investigated the biosynthesis of silver nanoparticles using Annona reticulata leaf aqueous extract. The biosynthesised silver nanoparticles were confirmed by visual observation and UV-Vis spectroscopy. Appearance of dark brown colour indicated the synthesis of silver in the reaction mixture. The silver nanoparticles were found to be spherical, rod, and triangular in shape with variable size ranging from 23.84 to 50.54 nm, as evident by X-ray diffraction studies, TEM. The X-ray diffraction studies, energy dispersive X-ray analysis, and TEM analysis indicate that the particles are crystalline in nature. The nanoparticles appeared to be associated with some chemical compounds which possess hydroxyl and carbonyl groups, confirmed by FTIR. This is the first and novel report of silver nanoparticles synthesised from Annona reticulata leaves extract and their antidermatophytic activity.

scholarly journals Syntheses, Crystal Structures, and Antibacterial Activity of New Tetranuclear Zinc(II) Complexes with Schiff Base Ligands

2021 ◽  
Vol 68 (3) ◽  
pp. 638-644
Author(s):  
Heng-Yu Qian
Keyword(s):  
Antibacterial Activities ◽  
Bacterial Strains ◽  
X Ray Diffraction ◽  
X Ray ◽  
E Coli ◽  
Mtt Method ◽  
Trigonal Bipyramidal ◽  
Vis Spectroscopy ◽  
Crystal Structural ◽  
L1 And L2

Two new tetranuclear zinc(II) complexes, [Zn4(L1)2(μ2-η1:η1-CH3COO)4(μ1,1-N3)2] (1) and [Zn4(L2)4(CH3CH2OH) (H2O)] (2), where L1 and L2 are the deprotonated forms of 4-fluoro-2-((pyridin-2-ylmethylimino)methyl)phenol (HL1) and 4-fluoro-2-((2-(hydroxymethyl)phenylimino)methyl)phenol (H2L2), have been synthesized and characterized by elemental analysis, IR and UV-vis spectroscopy, and single crystal X-ray diffraction. X-ray crystal structural study indicated that the distances between the adjacent Zn atoms are 3.160(1)–3.353(1) Å in 1 and 3.005(1)–3.168(1) Å in 2. All zinc atoms in 1 are pentacoordinated in trigonal bipyramidal geometry, and those in 2 are in square pyramidal and octahedral geometry. The complexes and the Schiff bases were assayed for antibacterial activities against three Gram-positive bacterial strains (B. subtilis, S. aureus, and St. faecalis) and three Gram-negative bacterial strains (E. coli, P. aeruginosa, and E. cloacae) by MTT method.

scholarly journals Synthesis of Silver Nanoparticles by Leaf Surface Fungi Isolated from Piper betle L. and its Antimicrobial Potential Study

2020 ◽  
Vol 2 (1) ◽  
pp. 46
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Surface ◽  
Agar Plate ◽  
Fungal Species ◽  
Piper Betle ◽  
Bacterial Strains ◽  
Plate Method ◽  
Synthesis Of Nanoparticles ◽  
Vis Spectroscopy ◽  
Postgraduate Studies

Studies on the biodiversity of phylloplane and endophytic fungi on the leaf surfaces of the medicinal plant; Piper betle L. was made in our Microbiology laboratory, K.M. Govt. Institute for Postgraduate Studies and Research (Autonomous), Puducherry. The Agar plate method was used to isolate both the leaf surface and sub-surface fungi. During the study period, altogether twelve fungal species of seven genera of phylloplane and five species of endophytes were isolated from Piper betle L. by agar plate method. Penicillium sp., a dominant fungus, was chosen for the synthesis of silver nanoparticles. Silver nanoparticles (AgNPs) were synthesized from Ag+ ions by treating with different extracts of the fungus with AgNO3.The appearance of yellowish-brown color in the conical flasks suggested the formation of AgNPs in dark and light conditions. The AgNPs were characterized by UV-Vis spectroscopy, which has proved to be very useful for the analysis of nanoparticles. Candida albicans was found most susceptible towards the AgNPs of the fungus in comparison to other bacterial strains. Among the bacterial strains, Staphylococcus aureus was more suffered than V. parahaemolyticus and E. coli. It was found in the present study that our process for the synthesis of nanoparticles was easy, safe, and economical to be readily used in the field of biomedicine.

scholarly journals Biosynthesis, characterisation and therapeutic applications of plant-mediated silver nanoparticles

2018 ◽  
Vol 83 (5) ◽  
pp. 515-538 ◽  
Author(s):  
Andreia Corciova ◽  
Bianca Ivanescu
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Nanoparticle Synthesis ◽  
Special Importance ◽  
X Ray Diffraction ◽  
X Ray ◽  
Stabilizing Agents ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Nanoparticles Synthesis

Nanotechnology is one of the most studied domains, and nanoparticle synthesis, especially of silver nanoparticles, has gained special importance due to their properties, biocompatibility and applications. Today, the processes of nanoparticles synthesis tend toward the development of inexpensive, simple, non-toxic and environmentally friendly methods. Thus, the use of plants in the synthesis of silver nanoparticles has attracted considerable interest because biomolecules can act as both reducing and stabilizing agents. This survey aims at discussing the conditions for obtaining silver nanoparticles using plants and their characterization by several methods, such as FTIR and UV?Vis spectroscopy, X-ray diffraction, and scanning and transmission electron microscopy. In addition, it examines some of the most common biological uses of silver nanoparticles: antibacterial, antioxidant and cytotoxic.

Synthesis of Silver Nanoparticles using Euphorbia wallichii Extract and Assessment of their Bio-functionalities

2020 ◽  
Vol 16 (4) ◽  
pp. 495-506 ◽  
Author(s):  
Abdul-Rehman Phull ◽  
Attarad Ali ◽  
Akhtar Ali ◽  
Sanaullah Abbasi ◽  
Muhammad Zia ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Brine Shrimp ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Cost Effective ◽  
Spectrometric Analysis ◽  
Bacterial Strains ◽  
X Ray ◽  
Synthesis Of Nanoparticles

Background: Silver nanoparticles synthesized by the bio-green method have been applied to various biomedical applications. These procedures are simple, eco-friendly and serve as an alternative to complex chemical methods for the preparation of nanomaterials. Objective: : In the present study, phytosynthesis of silver nanoparticles, to examine their antioxidant potential, toxic effects towards bacterial-, fungal-strains, brine shrimp nauplii and cancer cells was focused. Methods: Methanolic extract of Euphorbia wallichii roots was used for the synthesis of silver nanoparticles. The synthesis was monitored and confirmed by UV-visible spectroscopy, Fourier Transform Infra-Red (FTIR) spectrometric analysis, Field Emission Scanning Electron Microscope (FESEM), Energy Dispersive X-ray (EDX) and X-Ray Powder Diffraction (XRD). Results: The synthesized particles were average 63±8 nm in size. Involvement of phenolic (46.7±2.4 µg GAE/mg) and flavonoid (11.7±1.2 µg QE/mg) compounds as capping agents was also measured. Nanoparticles showed antioxidant properties in terms of free radical scavenging potential (59.63±1.0 %), reducing power (44.52±1.34 µg AAE/mg) and total antioxidant capacity (60.48±2.2 µg AAE/mg). The nanoparticles showed potent cytotoxic effects against brine shrimp nauplii (LD50 66.83 µg/ml), proliferation and cell death of HeLa cells as determined by MTT (LD50 0.3923 µg/ml) and TUNEL assays, respectively. Antimicrobial results revealed that silver nanoparticles were found to be more potent against pathogenic fungal (maximum active against A. fumigatus, MIC 15 µg/disc) and bacterial strains (maximum active against S. aureus, MIC 3.33 μg/disc) than the E. wallichii extract alone. Conclusion: These results support the advantages of using an eco-friendly and cost-effective method for synthesis of nanoparticles with antioxidant, cytotoxic and antimicrobial potential.

Flower-Mediated Phytosynthesis of Silver Nanoparticles from Butea monosperma Lam. and their Evaluation for Antibacterial Activity

2019 ◽  
Vol 12 (6) ◽  
pp. 4718-4723
Author(s):  
Dipika Rathod ◽  
Illa Patel ◽  
Priyanka Chaudhari ◽  
Anita Solanki Solanki
Keyword(s):  
Silver Nanoparticles ◽  
Metallic Nanoparticles ◽  
X Ray Diffraction ◽  
Butea Monosperma ◽  
X Ray ◽  
Synthesis Of Nanoparticles ◽  
Potential Source ◽  
Uv Visible ◽  
And Staphylococcus Aureus ◽  
Silver Metal

The green synthesis of metallic nanoparticles is the simplest, affordable and eco-friendly approach, which attracted researchers because of their immense applications. The plant-mediated synthesis of nanoparticles plays an important role in the field of nanobiotechnology as they devoid of harmful chemicals. Plenty of reports were available on synthesis of silver nanoparticles using the vegetative parts of plant especially foliar/leaf parts but reports on floral/flower parts utilized for silver nanoparticles synthesis were limited. Although flowers were found as potential source of many important phytochemicals which can be used for treatments of many diseases and Butea monosperma Lam. Flowers were utilized for curing several diseases so, here its extract were used for synthesis of silver nanoparticles by using it as capping and stabilizing agent. The present study, deals with synthesis of silver nanoparticles (AgNPs) from Butea monosperma Lam. Flower extracts through greener approach and then synthesized AgNPs were characterized using UV- visible spectrometry, X-Ray diffraction (XRD), Fourier transform infra-red (FTIR) spectroscopy and Scanning electron microscopy (SEM) which confirms its synthesis from silver metal. Further, the anti-bacterial properties of the synthesized silver nanoparticles were studied, and the results revealed that the flower mediated silver nanoparticles had showed strong anti-bacterial activity against Pseudomonas sp., Escherichia coli, Bacillus subtilis and Staphylococcus aureus.

scholarly journals Factors affecting synthesis of silver-nanoparticles and antimicrobial applications

2020 ◽  
Vol 129 (1D) ◽  
pp. 25-31
Author(s):  
Ton Nu My Phuong ◽  
Nguyen Thi Thanh Hai ◽  
Nguyen Thi Thu Thuy ◽  
Nguyen Vinh Phu ◽  
Nguyen Thi Huong ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Chemical Reduction ◽  
Nano Particles ◽  
Protective Agent ◽  
X Ray Diffraction ◽  
Factors Affecting ◽  
X Ray ◽  
Silver Sulfate ◽  
Vis Spectroscopy ◽  
Initial Ph

Silver nanoparticles were synthesized from silver sulfate by using the chemical reduction method with dextran as both a reducing agent and a protective agent. The influence of reaction temperature, time, and initial pH on the synthesis was investigated. The formation of Ag nano-particles (AgNPs) and their morphology were characterized with UV-Vis spectroscopy, X-ray diffraction, scanning electron microscopy, energy dispersive X-ray analysis, and Fourier transform-infrared spectroscopy. The antifungal and antibacterial effects of AgNPs/dextran on Xanthomonas oryzae and Pyricularia oryzae were tested.

scholarly journals Green Synthesis of Silver Nanoparticles using Strobilanthes flaccidifolius Nees. Leaf Extract and its Antibacterial Activity

2016 ◽  
Vol 8 (1) ◽  
pp. 1523-1532 ◽  
Author(s):  
Sujata D Wangkheirakpam ◽  
Wangkheirakpam Radhapiyari Devi ◽  
Chingakham Brajakishore Singh ◽  
Warjeet S Laitonjam
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Leaf Extract ◽  
X Ray Diffraction ◽  
Paramagnetic Resonance ◽  
X Ray ◽  
Vis Spectroscopy ◽  
Electron Paramagnetic Resonance Technique ◽  
Average Size ◽  
Green Technique

The leaf extract of Strobilanthes flaccidifolius Nees. was used for the synthesis of silver nanoparticles through a green technique of synthesis. The nanoparticles was characterized by UV-VIS spectroscopy which proves the formation silver nanoparticles. FTIR (Fourier Transmission infra red spectroscopy) study was carried out to assess the biomolecule as indigo precursors, Energy dispersion X-ray analysis(EDX) data further proves it. EPR (Electron paramagnetic resonance technique) shows the free radical in silver neutral state and XRD(X-ray diffraction technique) also repots silver neutral formation.The morphology and the shape of the silver nanoparticles were determined by Scanning electron microscopy(SEM) and Tunneling electron microscopy (TEM).The nanoparticles adopted spherical morphology and the size ranging from 6nm to 54.11nm and average size was determined as 12.15± 5.3nm.The nanoparticles had antimicrobial activity

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