scholarly journals Electroacupuncture at ST36 Increases Bone Marrow-Derived Interstitial Cells of Cajal via the SDF-1/CXCR4 and mSCF/Kit-ETV1 Pathways in the Stomach of Diabetic Mice

2018 ◽  
Vol 2018 ◽  
pp. 1-14
Author(s):  
Jiao Zhao ◽  
Jing An ◽  
Shi Liu
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplantation ◽  
High Frequency ◽  
Interstitial Cells Of Cajal ◽  
Low Frequency ◽  
Interstitial Cells ◽  
Diabetic Mice ◽  
Protein Levels ◽  
Qrt Pcr ◽  
Cells Of Cajal

Background. The loss of interstitial cells of Cajal (ICC) is observed in diabetic gastroparesis. Electroacupuncture (EA) maintains ICC networks, but the effects and mechanisms of EA on ICC of bone marrow derivation in the stomach have not been investigated. Methods. C57BL/6 mice were randomized into six groups: control, diabetic (DM), bone marrow transplantation (BMT) + DM, BMT + DM + sham EA (SEA), BMT + DM + low-frequency EA (LEA), and BMT + DM + high-frequency (HEA). c-Kit+GFP+ cells in the stomach were detected by immunofluorescence staining. Western blotting and qRT-PCR were employed to determine c-Kit, GFP, SDF-1, CXCR4, mSCF, pERK, and ETV1 expression. Results. (1) c-Kit+GFP+ cells were elevated in the BMT + DM + LEA and HEA groups. (2) The mRNA and protein levels of GFP, SDF-1, and CXCR4 were increased in the BMT + DM + LEA and BMT + DM + HEA groups. (3) The mRNA and protein levels of mSCF, c-Kit, pERK, and ETV1 were significantly reduced in the DM group but markedly elevated in the BMT + DM + LEA and HEA groups. Conclusion. EA at ST36 increases bone marrow-derived ICC in the stomach of diabetic mice via the SDF-1/CXCR4 and mSCF/c-Kit-ETV1 pathways.

scholarly journals Electroacupuncture at ST36 Protects ICC Networks via mSCF/Kit-ETV1 Signaling in the Stomach of Diabetic Mice

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Lugao Tian ◽  
Beibei Zhu ◽  
Shi Liu
Keyword(s):  
Gastric Emptying ◽  
High Frequency ◽  
Interstitial Cells Of Cajal ◽  
Low Frequency ◽  
Control Group ◽  
Diabetic Patients ◽  
Diabetic Mice ◽  
Protein Levels ◽  
Reliable Marker ◽  
Cells Of Cajal

Background. Electroacupuncture (EA) at ST36 has been used to regulate gastric motility and effectively improve gastric emptying in diabetic patients. Nevertheless, the specific mechanisms underlying the efficacy of this treatment remain unknown. The aim of this study was to assess the variations of interstitial cells of Cajal (ICC) and explore the changes in mSCF/KIT-ETV1 signaling in the antrum and corpus of diabetic mice after treatment with EA.Methods. Male C57BL/6 mice were randomized into five groups: control group, diabetic group (DM), diabetic-plus-sham EA group (SEA), diabetic-plus-low-frequency EA group (LEA), and diabetic-plus-high-frequency EA group (HEA). The expression levels of Ano1, c-Kit, and ETV1 were assessed by immunofluorescence in the antrum and corpus. Western blotting and PCR methods were further used to evaluate c-Kit, mSCF, and ETV1 expression.Results. (1) c-Kit and Ano1 were obviously decreased in the DM group, but c-Kit reduced much more than Ano1. (2) The mSCF, c-Kit, and ETV1 mRNA and protein levels were obviously decreased in the DM group in both the antrum and the corpus (P<0.01), but they were significantly elevated in the LEA and HEA groups (P<0.01).Conclusions. Ano1 is a reliable marker to detect ICC changes in diabetes; low- and high-frequency EA at acupoint ST36 can protect the networks of ICC possibly via normal activation of mSCF/KIT-ETV1 signaling.

scholarly journals Electroacupuncture Regulates Apoptosis/Proliferation of Intramuscular Interstitial Cells of Cajal and Restores Colonic Motility in Diabetic Constipation Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Juanjuan Xu ◽  
Yan Chen ◽  
Shi Liu ◽  
Xiaohua Hou
Keyword(s):  
High Frequency ◽  
Interstitial Cells Of Cajal ◽  
Colonic Motility ◽  
Low Frequency ◽  
Interstitial Cells ◽  
Colonic Transit ◽  
Immunofluorescent Staining ◽  
High Frequency Stimulation ◽  
Frequency Stimulation ◽  
Cells Of Cajal

Injury of interstitial cells of Cajal (ICC) is associated with gut dysmotility in diabetic rats. We have shown an acceleration of the colonic contractility by electroacupuncture stimulation (EAS). However, little is known about potential roles of EAS on colonic transit and ICC. In this study, we evaluate the effect of EAS on colonic transit and investigate whether apoptosis/proliferation of ICC was involved in regulative effect of EAS on colonic transit. Rats were randomly assigned to normal, diabetic, diabetic-plus-sham stimulation, diabetic-plus-low-frequency stimulation, and diabetic-plus-high-frequency stimulation groups. Bead expulsion test was used for measuring the distal colonic transit. The Kit (ICC marker) was detected by western blot. Apoptotic ICC was detected by terminal dUTP nucleotide end labeling. Proliferating ICC was identified by Kit/Ki67 double immunofluorescent staining on whole mount preparations. Ultrastructure changes of ICC were studied using electron microscopy. Results showed that high-frequency stimulation significantly promoted colonic transit. Low- and high-frequency stimulation markedly rescued intramuscular ICC from apoptosis. Abundant proliferating intramuscular ICC was found in low- and high-frequency stimulation groups. Our results indicate that high-frequency EAS has stimulatory effect on the distal colonic transit, which may be mediated by downregulation of the apoptosis and upregulation of the proliferation of intramuscular ICC.

Electroacupuncture atZusanli(ST-36) Restores Impaired Interstitial Cells of Cajal and Regulates Stem Cell Factor Pathway in the Colon of Diabetic Rats

2012 ◽  
Vol 17 (2) ◽  
pp. 117-125 ◽  
Author(s):  
Juanjuan Xu ◽  
Yan Chen ◽  
Shi Liu ◽  
Xiaohua Hou
Keyword(s):  
Stem Cell ◽  
High Frequency ◽  
Stem Cell Factor ◽  
Interstitial Cells Of Cajal ◽  
Low Frequency ◽  
Interstitial Cells ◽  
High Frequency Stimulation ◽  
Frequency Stimulation ◽  
Cells Of Cajal

The present study determined the effects of electroacupuncture on interstitial cells of Cajal and investigated whether changes in the stem cell factor pathway were involved. Animals were assigned to normal, diabetic, diabetic plus sham stimulation, diabetic plus low-frequency stimulation, and diabetic plus high-frequency stimulation groups. Electroacupuncture was performed daily for 8 weeks. In vitro contractility of colonic muscle strips were studied. Expression of c-kit (the marker of interstitial cells of Cajal) and stem cell factor were measured. The results showed that (1) contraction of colonic muscle strips was significantly elevated in low- and high-frequency stimulation groups and (2) in contrast to the diabetic group, the expressions of c-kit and stem cell factor were markedly increased in the low- and high-frequency stimulation groups. These results indicate that both low- and high-frequency stimulation can promote the contractility of colonic muscle strips partially through increasing the number of interstitial cells of Cajal, and these effects could be mediated by an elevated endogenous stem cell factor.

scholarly journals Homing of the Bone Marrow-Derived Interstitial Cells of Cajal is Decreased in Diabetic Mouse Intestine

2011 ◽  
Vol 26 (6) ◽  
pp. 1072-1078 ◽  
Author(s):  
Yimin Li ◽  
Hideto Kojima ◽  
Kazunori Fujino ◽  
Kazuhiro Matsumura ◽  
Miwako Katagi ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Interstitial Cells Of Cajal ◽  
Diabetic Mouse ◽  
Interstitial Cells ◽  
Mouse Intestine ◽  
Cells Of Cajal

T1363 Effects of Blood Glucose and Insulin On Interstitial Cells of Cajal in Diabetic Mice Colon

2008 ◽  
Vol 134 (4) ◽  
pp. A-539
Author(s):  
Lin Lin ◽  
Yurong Tang ◽  
Liming Xu ◽  
Li Xueliang ◽  
Hongjie Zhang ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Diabetic Mice ◽  
Cells Of Cajal

T1364 The Effect of Stem Cell Factor On Interstitial Cells of Cajal in the Colon of Diabetic Mice

2008 ◽  
Vol 134 (4) ◽  
pp. A-539-A-540
Author(s):  
Lin Lin ◽  
Liming Xu ◽  
Yurong Tang ◽  
Hongjie Zhang ◽  
Li Xueliang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Factor ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Diabetic Mice ◽  
Cells Of Cajal
Sign in / Sign up

Export Citation Format

Share Document