scholarly journals Corrigendum to “Cerium Oxide Nanoparticles Induced Toxicity in Human Lung Cells: Role of ROS Mediated DNA Damage and Apoptosis”

2018 ◽  
Vol 2018 ◽  
pp. 1-3 ◽  
Author(s):  
Sandeep Mittal ◽  
Alok K. Pandey
Keyword(s):  
Dna Damage ◽  
Cerium Oxide ◽  
Human Lung ◽  
Cerium Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Lung Cells ◽  
Human Lung Cells

scholarly journals Cerium Oxide Nanoparticles Induced Toxicity in Human Lung Cells: Role of ROS Mediated DNA Damage and Apoptosis

2014 ◽  
Vol 2014 ◽  
pp. 1-14 ◽  
Author(s):  
Sandeep Mittal ◽  
Alok K. Pandey
Keyword(s):  
Dna Damage ◽  
Cell Death ◽  
Cerium Oxide ◽  
Oxidative Dna Damage ◽  
Apoptotic Cell ◽  
Morphological Changes ◽  
A549 Cells ◽  
Cerium Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Human Lung Cells

Cerium oxide nanoparticles (CeO2NPs) have promising industrial and biomedical applications. In spite of their applications, the toxicity of these NPs in biological/physiological environment is a major concern. Present study aimed to understand the molecular mechanism underlying the toxicity of CeO2NPs on lung adenocarcinoma (A549) cells. After internalization, CeO2NPs caused significant cytotoxicity and morphological changes in A549 cells. Further, the cell death was found to be apoptotic as shown by loss in mitochondrial membrane potential and increase in annexin-V positive cells and confirmed by immunoblot analysis of BAX, BCl-2, Cyt C, AIF, caspase-3, and caspase-9. A significant increase in oxidative DNA damage was found which was confirmed by phosphorylation of p53 gene and presence of cleaved poly ADP ribose polymerase (PARP). This damage could be attributed to increased production of reactive oxygen species (ROS) with concomitant decrease in antioxidant “glutathione (GSH)” level. DNA damage and cell death were attenuated by the application of ROS and apoptosis inhibitors N-acetyl-L- cysteine (NAC) and Z-DEVD-fmk, respectively. Our study concludes that ROS mediated DNA damage and cell cycle arrest play a major role in CeO2NPs induced apoptotic cell death in A549 cells. Apart from beneficial applications, these NPs also impart potential harmful effects which should be properly evaluated prior to their use.

scholarly journals Polyphosphate Reverses the Toxicity of the Quasi-Enzyme Bleomycin on Alveolar Endothelial Lung Cells In Vitro

Cancers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 750
Author(s):  
Werner E. G. Müller ◽  
Meik Neufurth ◽  
Shunfeng Wang ◽  
Heinz C. Schröder ◽  
Xiaohong Wang
Keyword(s):  
Dna Damage ◽  
Human Lung ◽  
Dna Integrity ◽  
Lung Cells ◽  
Antitumor Antibiotic ◽  
Cell Toxicity ◽  
Inorganic Polyphosphate ◽  
Human Lung Cells ◽  
Anti Cancer

The anti-cancer antitumor antibiotic bleomycin(s) (BLM) induces athyminic sites in DNA after its activation, a process that results in strand splitting. Here, using A549 human lung cells or BEAS-2B cells lunc cells, we show that the cell toxicity of BLM can be suppressed by addition of inorganic polyphosphate (polyP), a physiological polymer that accumulates and is released from platelets. BLM at a concentration of 20 µg ml−1 causes a decrease in cell viability (by ~70%), accompanied by an increased DNA damage and chromatin expansion (by amazingly 6-fold). Importantly, the BLM-caused effects on cell growth and DNA integrity are substantially suppressed by polyP. In parallel, the enlargement of the nuclei/chromatin in BLM-treated cells (diameter, 20–25 µm) is normalized to ~12 µm after co-incubation of the cells with BLM and polyP. A sequential application of the drugs (BLM for 3 days, followed by an exposure to polyP) does not cause this normalization. During co-incubation of BLM with polyP the gene for the BLM hydrolase is upregulated. It is concluded that by upregulating this enzyme polyP prevents the toxic side effects of BLM. These data might also contribute to an application of BLM in COVID-19 patients, since polyP inhibits binding of SARS-CoV-2 to cellular ACE2.

scholarly journals Role of trivalent La and Nd dopants in lattice distortion and oxygen vacancy generation in cerium oxide nanoparticles

2006 ◽  
Vol 88 (24) ◽  
pp. 243110 ◽  
Author(s):  
Swanand Patil ◽  
Sudipta Seal ◽  
Yu Guo ◽  
Alfons Schulte ◽  
John Norwood
Keyword(s):  
Oxygen Vacancy ◽  
Cerium Oxide ◽  
Lattice Distortion ◽  
Cerium Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Vacancy Generation

scholarly journals Ceramide Synthases Expression and Role of Ceramide Synthase-2 in the Lung: Insight from Human Lung Cells and Mouse Models

PLoS ONE ◽  
2013 ◽  
Vol 8 (5) ◽  
pp. e62968 ◽  
Author(s):  
Irina Petrache ◽  
Krzysztof Kamocki ◽  
Christophe Poirier ◽  
Yael Pewzner-Jung ◽  
Elad L. Laviad ◽  
...  
Keyword(s):  
Mouse Models ◽  
Human Lung ◽  
Lung Cells ◽  
Ceramide Synthase ◽  
Human Lung Cells ◽  
Ceramide Synthases
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