scholarly journals Undetectable role of oxidative DNA damage in cell cycle, cytotoxic and clastogenic effects of Cr(VI) in human lung cells with restored ascorbate levels

Mutagenesis ◽  
2012 ◽  
Vol 27 (4) ◽  
pp. 437-443 ◽  
Author(s):  
M. Reynolds ◽  
S. Armknecht ◽  
T. Johnston ◽  
A. Zhitkovich
Keyword(s):  
Cell Cycle ◽  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Human Lung ◽  
Lung Cells ◽  
Human Lung Cells

scholarly journals Polyphosphate Reverses the Toxicity of the Quasi-Enzyme Bleomycin on Alveolar Endothelial Lung Cells In Vitro

Cancers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 750
Author(s):  
Werner E. G. Müller ◽  
Meik Neufurth ◽  
Shunfeng Wang ◽  
Heinz C. Schröder ◽  
Xiaohong Wang
Keyword(s):  
Dna Damage ◽  
Human Lung ◽  
Dna Integrity ◽  
Lung Cells ◽  
Antitumor Antibiotic ◽  
Cell Toxicity ◽  
Inorganic Polyphosphate ◽  
Human Lung Cells ◽  
Anti Cancer

The anti-cancer antitumor antibiotic bleomycin(s) (BLM) induces athyminic sites in DNA after its activation, a process that results in strand splitting. Here, using A549 human lung cells or BEAS-2B cells lunc cells, we show that the cell toxicity of BLM can be suppressed by addition of inorganic polyphosphate (polyP), a physiological polymer that accumulates and is released from platelets. BLM at a concentration of 20 µg ml−1 causes a decrease in cell viability (by ~70%), accompanied by an increased DNA damage and chromatin expansion (by amazingly 6-fold). Importantly, the BLM-caused effects on cell growth and DNA integrity are substantially suppressed by polyP. In parallel, the enlargement of the nuclei/chromatin in BLM-treated cells (diameter, 20–25 µm) is normalized to ~12 µm after co-incubation of the cells with BLM and polyP. A sequential application of the drugs (BLM for 3 days, followed by an exposure to polyP) does not cause this normalization. During co-incubation of BLM with polyP the gene for the BLM hydrolase is upregulated. It is concluded that by upregulating this enzyme polyP prevents the toxic side effects of BLM. These data might also contribute to an application of BLM in COVID-19 patients, since polyP inhibits binding of SARS-CoV-2 to cellular ACE2.

scholarly journals Ceramide Synthases Expression and Role of Ceramide Synthase-2 in the Lung: Insight from Human Lung Cells and Mouse Models

PLoS ONE ◽  
2013 ◽  
Vol 8 (5) ◽  
pp. e62968 ◽  
Author(s):  
Irina Petrache ◽  
Krzysztof Kamocki ◽  
Christophe Poirier ◽  
Yael Pewzner-Jung ◽  
Elad L. Laviad ◽  
...  
Keyword(s):  
Mouse Models ◽  
Human Lung ◽  
Lung Cells ◽  
Ceramide Synthase ◽  
Human Lung Cells ◽  
Ceramide Synthases

Acute exposure of human lung cells to 1,3-butadiene diepoxide results in G1 and G2 cell cycle arrest

2005 ◽  
Vol 45 (4) ◽  
pp. 354-364 ◽  
Author(s):  
Michael Schmiederer ◽  
Eugene Knutson ◽  
Perpetua Muganda ◽  
Thomas Albrecht
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Human Lung ◽  
Acute Exposure ◽  
Lung Cells ◽  
Cycle Arrest ◽  
Human Lung Cells ◽  
G2 Cell Cycle Arrest

Role of Nrf2 in preventing oxidative stress induced chloride current alteration in human lung cells

2018 ◽  
Vol 233 (8) ◽  
pp. 6018-6027 ◽  
Author(s):  
Rita Canella ◽  
Mascia Benedusi ◽  
Marta Martini ◽  
Franco Cervellati ◽  
Carlotta Cavicchio ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Human Lung ◽  
Chloride Current ◽  
Lung Cells ◽  
Human Lung Cells
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