scholarly journals Influence of Ultrapulsed CO2 Laser, before Application of Different Types of Fluoride, on the Increase of Microhardness of Enamel In Vitro

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Marcia Regina Cabral Oliveira ◽  
Pedro Henrique Cabral Oliveira ◽  
Luiz Henrique Cabral Oliveira ◽  
Ravana Angelini Sfalcin ◽  
Renato Araujo Prates ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Co2 Laser ◽  
Tooth Enamel ◽  
The Other ◽  
Fluoride Varnish ◽  
Fluoride Treatment ◽  
Enamel Caries ◽  
Bovine Enamel ◽  
Caries Lesions

Objective. To evaluate the influence of ultrapulsed CO2 laser in combination with commercial fluoride products in order to verify the increase of microhardness of artificial enamel caries lesions. Materials and Methods. Bovine enamel specimens were prepared, and artificial enamel caries lesions were created. Teeth were randomly divided into 5 groups (n=10): treated with laser (L), laser + neutral fluoride gel 2% (LNF), laser + acidulated phosphate fluoride gel 1.23% (LAFG), laser + acidulated fluoride mousse 1.23% (LAFM), and laser + fluoride varnish 5% (LFV). Microhardness was evaluated at baseline, after caries induction, after CO2 laser irradiation + fluoride treatment in the 1st week, and after fluoride treatment at 3rd and 5th week. Results. There was a decrease in microhardness in all groups after artificial enamel caries lesion formation; no increase in microhardness was found in the first and third weeks in all groups (p > 0.05). In the fifth week, an increase in microhardness occurred in all groups (p < 0.05). Conclusion. Although CO2 laser irradiation in combination with different commercial fluoride products was capable of increasing microhardness on enamel caries lesions in bovine tooth enamel it is necessary to confirm these results by testing the isolated effect of fluoride on enamel surface microhardness. Also, although microhardness was higher in the fluoride varnish group than in the other groups in the fifth week it is not possible to discard the best effect of fluoride varnish treatment on absence of artifacts that may occur with the other fluoride treatments. Clinical Relevance. In order to prove that CO2 laser may contribute to an increase in microhardness when applied to enamel lesions in combination with different commercial fluoride products it is necessary to conduct additional studies. Also, higher microhardness of fluoride varnish group should be carefully considered.

scholarly journals Comparative evaluation of microhardness and morphology of permanent tooth enamel surface after laser irradiation and fluoride treatment - An in vitro study

LASER THERAPY ◽  
2016 ◽  
Vol 25 (3) ◽  
pp. 201-208 ◽  
Author(s):  
Puneet Kumar ◽  
Mridula Goswami ◽  
Jatinder Kaur Dhillon ◽  
Ferah Rehman ◽  
Deepti Thakkar ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Comparative Evaluation ◽  
Tooth Enamel ◽  
Permanent Tooth ◽  
Enamel Surface ◽  
Fluoride Treatment

Evaluation of Primary Tooth Enamel Surface Morphology and Microhardness after Nd: YAG Laser Irradiation and APF Gel Treatment—An in vitro study

2011 ◽  
Vol 35 (4) ◽  
pp. 377-382 ◽  
Author(s):  
Naveen Reddy Banda ◽  
Vanaja Reddy G ◽  
N Shashikiran
Keyword(s):  
Laser Irradiation ◽  
Tooth Enamel ◽  
Morphological Changes ◽  
Enamel Surface ◽  
Permanent Teeth ◽  
Primary Tooth ◽  
Control Group ◽  
Yag Laser ◽  
Fluoride Treatment

Objective: Laser irradiation and fluoride has been used as a preventive tool to combat dental caries in permanent teeth, but little has been done for primary teeth which are more prone to caries. The purpose of this study was to evaluate microhardness alterations in the primary tooth enamel after Nd-YAG laser irradiation alone and combined with topical fluoride treatment either before or after Nd-YAG laser irradiation.Method: Ten primary molars were sectioned and assigned randomly to: control group, Nd-YAG laser irradiation,Nd-YAG lasing before APF and APF followed by Nd-YAG lasing. The groups were evaluated for microhardness. Surface morphological changes were observed using SEM. Results: Statistical comparisons were performed. The control group's SEM showed a relatively smooth enamel surface and lasing group had fine cracks and porosities. In the lasing + fluoride group a homogenous confluent surface was seen. In the fluoride + lasing group an irregular contour with marked crack propagation was noted. There was a significant increase in the microhardness of the treatment groups. Conclusion: Nd-YAG laser irradiation and combined APF treatment of the primary tooth enamel gave morphologically hardened enamel surface which can be a protective barrier against a cariogenic attack

Microhardness and SEM after CO2 laser irradiation or fluoride treatment in human and bovine enamel

2010 ◽  
Vol 73 (11) ◽  
pp. 1030-1035 ◽  
Author(s):  
A.E. Souza-Gabriel ◽  
V. Colucci ◽  
C.P. Turssi ◽  
M.C. Serra ◽  
S.A.M. Corona
Keyword(s):  
Laser Irradiation ◽  
Co2 Laser ◽  
Fluoride Treatment ◽  
Bovine Enamel

scholarly journals Effects of Self-Assembling Peptide P11-4, Fluorides, and Caries Infiltration on Artificial Enamel Caries Lesions in vitro

2017 ◽  
Vol 51 (5) ◽  
pp. 451-459 ◽  
Author(s):  
Richard J. Wierichs ◽  
Julian Kogel ◽  
Julian Lausch ◽  
Marcella Esteves-Oliveira ◽  
Hendrik Meyer-Lueckel
Keyword(s):  
T Test ◽  
Enamel Caries ◽  
Low Viscosity ◽  
Self Assembling ◽  
Oral Environment ◽  
Ph Cycling ◽  
Bovine Enamel ◽  
Caries Lesions ◽  
Paired T Test

The application of a self-assembling peptide on noncavitated caries lesions is supposed to be a feasible approach to facilitate remineralization and mask their unfavorable appearance. However, demineralizing conditions are common in the oral environment, so the aim of this pH-cycling study was to compare recommended and novel treatment methods regarding their ability to hamper demineralization and as a consequence mask artificial enamel caries lesions. Artificial caries lesions were prepared in bovine enamel and randomly allocated to 11 groups (n = 22). Treatments before pH-cycling were as follows: the application of a self-assembling peptide (Curodont™ Repair [C]), a low-viscosity resin (Icon® [I]), 2 fluoride solutions (10,000 ppm F-: Elmex fluid [E] and 43,350 ppm F-: Tiefenfluorid® [T]), and no intervention (N). During pH-cycling (28 days, 6 × 60 min demineralization/day) half of the specimens in each group were brushed (10 s; 2 ×/day) with either fluoride-free (named e.g., C0) or NaF (1,100 ppm F-; e.g., C1) dentifrice slurry. In another subgroup specimens were pH-cycled but not brushed (NNB). Differences in integrated mineral loss (ΔΔZ), lesion depth (ΔLD), and colorimetric values (ΔΔE) were calculated between values after pre-demineralization, surface treatment, and pH-cycling. Specimens of C0, C1, NNB, N0, N1, T0, and E0 showed significantly increased ΔZ and LD values after pH-cycling (p ≤ 0.003; paired t test). C0, C1, NNB, and N0 showed significantly higher changes in ΔΔZ than E1, I0, I1, and T1 (p < 0.001; ANOVA). Significantly reduced colorimetric values could only be observed for I1, I0, E1, and E0 after treatment and after pH-cycling (p ≤ 0.027; paired t test). In conclusion, under the conditions chosen only the application of a low-viscosity resin could mask caries lesions significantly, whereas self-assembling peptides could neither inhibit lesion progression nor mask the lesions considerably.

scholarly journals Artificial Caries Lesion Characteristics after Secondary Demineralization with Theobromine-Containing Protocol

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 300
Author(s):  
Hani M. Nassar ◽  
Frank Lippert
Keyword(s):  
Mineral Content ◽  
Significance Level ◽  
Lesion Depth ◽  
Caries Lesion ◽  
Mineral Profile ◽  
Bonferroni Test ◽  
Bovine Enamel ◽  
Caries Lesions ◽  
Nail Varnish

Developing artificial caries lesions with varying characteristics is needed to adequately study caries process in vitro. The objective of this study was to investigate artificial caries lesion characteristics after secondary demineralization protocol containing theobromine and fluoride. Sixty bovine enamel slabs (4 × 3 mm) were demineralized using a Carbopol-containing protocol for 6 days. A baseline area (2 × 3 mm) was protected with acid-resistant nail varnish, after which specimens were exposed for 24 h to a secondary demineralization protocol containing acetic acid plus one of four fluoride/theobromine combinations (n = 15): theobromine (50 or 200 ppm) and fluoride (0 or 1 ppm). Specimens were sectioned and analyzed using transverse microradiography for changes in mineral content, lesion depth, and surface layer mineralization. Data was analyzed using paired t-test and analysis of variance followed by Bonferroni test at 0.05 significance level. After secondary demineralization, fluoride-containing groups had significantly deeper lesions (p = 0.002 and 0.014) compared to the group with 0 ppm fluoride and 50 ppm theobromine. Mineral content and lesion depth were significantly different compared to baseline for all groups. Theobromine did not show an added effect on mineral uptake. Theobromine-containing groups exhibited particularly deep lesions with a more uniform mineral profile in the presence of fluoride.

Mineral Density and Fluoride Content of in vitro Remineralized Lesions

1988 ◽  
Vol 67 (3) ◽  
pp. 577-581 ◽  
Author(s):  
Y. Jima ◽  
T. Koulourides
Keyword(s):  
Complete Recovery ◽  
Fluoride Content ◽  
Mineral Density ◽  
Acid Dissolution ◽  
Thin Sections ◽  
Partially Saturated ◽  
In Vitro Investigation ◽  
Bovine Enamel ◽  
Caries Lesions

This in vitro investigation studied the remineralization of experimental caries lesions in bovine enamel by use of three methods: (1) surface microhardness, (2) microradiography, and (3) abrasion biopsy for mineral density and fluoride content. The lesions were produced by a two-day exposure to 0.01 mol/L lactic acidlsodium hydroxide buffer partially saturated with 3.0 mmol/L Ca, 1.8 mmol/L P, in 1% CMC, at pH 4.0 and 37°C. The lesions were exposed to a remineralizing solution containing 3.0 mmol/L Ca, 1.8 mmol/L P, and 3 ppm F in 1% CMC at pH 7.0 and 37°C for two, six, and ten days, with solution changes every two days. The data derived from the three methods are presented in sequence from the baseline and at days two, six, and ten of the remineralizing treatment. Microhardness measurements showed hardness recoveries of 35.9, 78.9, and 87.5%, respectively. Microradiography suggested complete recovery with the ten-day remineralization. Abrasion biopsy of successive 10-μm layers to a depth of 100 μm indicated 15.2, 39.8, and 68.8% mineral density recoveries, with fluoride content of the surface layer increasing from a baseline of 300 ppm to 4600, 9000, and 9800 ppm F for the 2, 6, 10 days of remineralization, respectively. Subsequent acid-etching of thin sections from the ten-day-remineralized specimens showed that the fluoride-enriched remineralized area was more resistant to acid dissolution than was the underlying nonnal enamel.

scholarly journals Microleakage of "Bulk-Fill" Composite Resin for Class II Restorations Pretreated With CO2 Laser in Deciduous Molars: An In Vitro Study

2019 ◽  
Vol 10 (4) ◽  
pp. 304-309
Author(s):  
Larissa Costa Santos ◽  
Ravana Angelini Sfalcin ◽  
Eugenio José Garcia ◽  
Fátima Antônia Aparecida Zanin ◽  
Aldo Brugnera Junior ◽  
...  
Keyword(s):  
Phosphoric Acid ◽  
Laser Irradiation ◽  
Co2 Laser ◽  
Class Ii ◽  
Composite Resins ◽  
Acid Etching ◽  
Dentin Hypersensitivity ◽  
Significant Difference ◽  
Acid Gel

Introduction: Microleakage has been reported to cause dentin hypersensitivity because of the passage of bacteria and their products through the restoration-tooth interface and is one of the main reasons for replacement of restorations. CO2 laser can be used for treatment of dentin hypersensitivity. Thus, this study aimed to evaluate in vitro the microleakage in composite restorations following surface pretreatment with acid etching and CO2 laser. Methods: Twelve human caries-free primary molars were selected. Class II cavities were prepared on occlusal mesial and occlusal distal surfaces. Specimens were randomly divided into four groups (n=6): Group 1 (G1) – 37% phosphoric acid gel etching + Beautiful-Bulk Restorative – Giomer (Shofu Inc); Group 2 (G2) – 37% phosphoric acid gel etching + SDR Bulk-Fill Flow (Dentsply); Group 3 (G3) – CO2 laser irradiation + Beautiful-Bulk Restorative – Giomer (Shofu Inc); Group 4 (G4) – CO2 laser irradiation + SDR Bulk-Fill Flow (Dentsply). Surfaces were restored with bonding agent (Natural Bond DE, DFL). Specimens were cut longitudinally and immersed in 0.5% methylene blue solution for 4 hours. Microleakage scores were assessed under a magnifying glass at x3,5 and qualitatively analyzed by scanning electron microscope (SEM). Data were analyzed using nonparametric Wilcoxon test (P < 0.05). Results: Scores prevailed between 0 and 2, however, no statistically significant difference was found among the groups (P= 0.05). Conclusion: It could be concluded that all composite resins bulk fill did not show significant difference among them regarding microleakage using either CO2 laser or 37% phosphoric acid etching.

Effect of xylitol varnishes on remineralization of artificial enamel caries lesions in vitro

2014 ◽  
Vol 42 (11) ◽  
pp. 1495-1501 ◽  
Author(s):  
C.A.B. Cardoso ◽  
A.R.F. de Castilho ◽  
P.M.A. Salomão ◽  
E.N. Costa ◽  
A.C. Magalhães ◽  
...  
Keyword(s):  
Enamel Caries ◽  
Caries Lesions

scholarly journals Remineralization of enamel caries by an amelogenin-derived peptide and fluoride in vitro

2020 ◽  
Vol 7 (3) ◽  
pp. 283-292 ◽  
Author(s):  
Longjiang Ding ◽  
Sili Han ◽  
Kun Wang ◽  
Sainan Zheng ◽  
Wenyue Zheng ◽  
...  
Keyword(s):  
Tooth Enamel ◽  
The Novel ◽  
Oral Diseases ◽  
Surface Microhardness ◽  
Enamel Caries ◽  
Low Concentrations ◽  
The World ◽  
Mineral Loss ◽  
Hydroxyapatite Crystals

Abstract Dental caries is one of the most common oral diseases in the world. This study was tantamount to investigate the combinatory effects of an amelogenin-derived peptide (called QP5) and fluoride on the remineralization of artificial enamel caries. The peptide QP5 was synthesized and characterized, and the binding capability of the peptide on hydroxyapatite (HA) and demineralized tooth enamel surface was analysed. Then, the mineralization function of the peptide and fluoride was studied through the spontaneous mineralization testing and remineralization on enamel caries in vitro. First, the novel peptide QP5 could bind on the hydroxyapatite and demineralized tooth enamel surfaces. Second, QP5 can transitorily stabilize the formation of amorphous calcium phosphate and direct the transformation into hydroxyapatite crystals alone and in combination with fluoride. In addition, compared to blocks treated by peptide QP5 alone or fluoride, the sample blocks showed significantly higher surface microhardness, lower mineral loss and shallower lesion depth after treatment with a combination of QP5 and fluoride at high or low concentrations. The peptide QP5 could control the crystallization of hydroxyapatite, and combinatory application of peptide QP5 and fluoride had a potential synergistic effect on the remineralization of enamel caries.

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