scholarly journals Development of Novel Single-Chain Antibodies against the Hydrophobic HPV-16 E5 Protein

2018 ◽  
Vol 2018 ◽  
pp. 1-7
Author(s):  
César Monjarás-Ávila ◽  
Sofía Bernal-Silva ◽  
Horacio Bach
Keyword(s):  
Cell Transformation ◽  
Fluorescent Microscopy ◽  
Hpv Infection ◽  
Human Papilloma Virus Type ◽  
Single Chain ◽  
Hpv 16 ◽  
Single Chain Antibodies ◽  
E5 Protein ◽  
High Prevalence ◽  
Small Hydrophobic Protein

The human papilloma virus type 16 infects genital mucosa with high prevalence in the oncogenesis of cervical and oropharyngeal cancers. The E5 protein of this virus is a small hydrophobic protein, whose expression generally decreases as the infection progresses to malignancy. These characteristics point to a role of E5 in the establishment of HPV infection and the initiation into cell transformation. The study of the HPV-16 E5 functions has been hindered because of the lack of antibodies. Detection is very difficult because of its hydrophobic nature, membrane location, and very low levels of expression. Thus, the objective of this study was to select single-chain antibodies against the full size E5 protein, which was coexpressed with maltose-binding protein. We report that the E5 protein was recognized by the antibody and was validated in W12 cells by fluorescent microscopy, including a colocalization with one of its host substrates. The use of this antibody could increase our knowledge about the functions of the oncogenic HPV-16 E5 protein during the earliest stages of keratinocyte infection in human.

scholarly journals Infección por Virus del Papiloma Humano y citología cérvico-vaginal en mujeres indígenas del Cañar, Ecuador.

Bionatura ◽  
2019 ◽  
Vol 4 (3) ◽  
pp. 934-938
Author(s):  
Julia Irma Carrión Ordoñez ◽  
Yudira Soto Brito ◽  
Maritza Pupo Antúnez ◽  
Rita Loja Chango
Keyword(s):  
Hpv Infection ◽  
Cross Sectional Study ◽  
Single Women ◽  
Hormonal Contraceptives ◽  
Cervical Lesions ◽  
Cross Sectional ◽  
Hpv 16 ◽  
Papilloma Virus ◽  
High Prevalence ◽  
Cytological Alterations

There are few studies on the circulation of Human Papilloma Virus (HPV) in indigenous Ecuadorian women. The aim of the study is to determine the circulation of HPV and identify cytological alterations in cervical samples of indigenous Ecuadorian women and to define the behavior of some socio-demographic and clinical-epidemiological variables. An analytical cross-sectional study was done between July 2017 and September 2018 to determine the presence of cytological alterations and HPV infection in 100 indigenous women between 15 and 55 years of age, residing in Cañar, Ecuador. The association between socio-demographic and clinical-epidemiological variables with viral infection was investigated. Was obtained a 98% of negative cytology for malignancy and 2% of cervical lesions. Cases with high-grade lesions were not diagnosed. In general, a 34% (34/100) tested was positive for HPV, predominating oncogenic genotypes. HPV 31 was the most frequent in 41.2% (14/34) of cases followed by HPV 16 in 20.6% (7/34). Women between 20 and 30 years of age were five times more likely to be infected with HPV (44.1%, 15/34). The frequency of infection was significantly higher in single women and in those who reported having 2 to 3 births. Infection with HPV 16 was associated with the use of hormonal contraceptives, in 57.1% (4/7) of the cases; p = 0.005, RP = 12.44 IC95% (2.40-64.62). The high prevalence of oncogenic HPV infection indicates the need to incorporate this indigenous population into early detection programs for cervical cancer.

scholarly journals High Prevalence of Human Papillomavirus in Colorectal Cancer in Hispanics: A Case-Control Study

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Raul D. Bernabe-Dones ◽  
Maria Gonzalez-Pons ◽  
Alejandro Villar-Prados ◽  
Mercedes Lacourt-Ventura ◽  
Heriberto Rodríguez-Arroyo ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Case Control Study ◽  
Hpv Infection ◽  
Case Control ◽  
Colorectal Carcinogenesis ◽  
Colorectal Tumors ◽  
Hpv 16 ◽  
High Prevalence ◽  
Control Study

The role of Human Papillomavirus (HPV) in colorectal carcinogenesis remains elusive. Based on the high incidence of HPV-associated malignancies among Puerto Rican Hispanics, this study aimed to assess the prevalence of HPV infection and viral integration in colorectal tissues in order to evaluate its putative role in colorectal cancer (CRC). In this case-control study, the prevalence of HPV infection in CRC (casesn= 45) and normal colon mucosa from cancer-free subjects (controlsn= 36) was assessed by a nested PCR strategy. HPV-16 genotyping was performed in HPV-positive tissues and the physical status of the HPV-16 genome was determined by E2 detection. HPV was detected in 19 of 45 (42.2%) CRC cases (mean age 61.1 ± 10.7 years, 24 males) and in 1 of 36 (2.8%) controls (mean age 60.9 ± 9.6 years, 24 males) with an OR = 25.58 (95% CI 3.21 to 203.49). HPV-16 was detected in 63.2% of the HPV-positive colorectal tumors; genome integration was observed in all HPV-16 positive cases. This is the first report showing the high prevalence of HPV infections in Caribbean Hispanic colorectal tumors. Despite evidence of HPV integration into the host genome, further mechanistic analysis examining HPV oncoprotein expression and the putative role of these oncoproteins in colorectal carcinogenesis is warranted.

scholarly journals High prevalence of human papillomavirus and European variants of HPV 16 infecting concomitantly to cervix and oral cavity in HIV positive women

PLoS ONE ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. e0227900
Author(s):  
Milagros Pérez-Quintanilla ◽  
Rocío Méndez-Martínez ◽  
Salvador Vázquez-Vega ◽  
Raquel Espinosa-Romero ◽  
Rita Sotelo-Regil ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Oral Cavity ◽  
Hiv Positive ◽  
Hpv 16 ◽  
Hiv Positive Women ◽  
High Prevalence

scholarly journals Identification of single chain antibodies to breast cancer stem cells using phage display

2009 ◽  
pp. NA-NA ◽  
Author(s):  
Deniz Gur ◽  
Suling Liu ◽  
Anurag Shukla ◽  
Stephanie C Pero ◽  
Max S Wicha ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Phage Display ◽  
Breast Cancer Stem Cells ◽  
Single Chain ◽  
Single Chain Antibodies

Optimization of the expression of single-chain antibodies using different Escherichia coli systems

2007 ◽  
Vol 131 (2) ◽  
pp. S251-S252
Author(s):  
Symon Riedstra ◽  
Gonçalo Leite ◽  
Carolina Ferreira ◽  
Francisco Brás Gomes ◽  
Paulo M.P. Costa ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Single Chain ◽  
Single Chain Antibodies

Vroegtijdige opsporing van voorstadia van een anaal carcinoom bij hiv-seropositieve patiënten

2021 ◽  
Author(s):  
E. GÖKCE ◽  
J. GELDOF ◽  
P. ROELANDT ◽  
J. VAN DORPE ◽  
S. CALLENS ◽  
...  
Keyword(s):  
Anal Cancer ◽  
Digital Rectal Examination ◽  
Intraepithelial Neoplasia ◽  
Hpv Infection ◽  
High Grade ◽  
Anal Squamous Cell Carcinoma ◽  
Increased Risk ◽  
High Prevalence ◽  
Risk Patients ◽  
Hiv Seropositive

Early detection of precursor lesions of anal cancer in HIV-seropositive patients Although anal cancer is rare in the overall population, its incidence is increasing in the last decades. Especially HIV-seropositive patients have an increased risk of developing anal squamous cell carcinoma (SCC), mainly because of the high prevalence of high-grade anal intraepithelial neoplasia (AIN) among these patients. High-grade AIN is a precursor lesion for anal SCC associated with human papillomavirus (HPV) infection. Despite the lack of direct evidence demonstrating that AIN identification reduces the risk of anal cancer, experts think that screening and treatment of high-risk patients will prevent the disease. This article aims to review the current literature about AIN and discusses the screening options, including digital rectal examination, anal cytology and high-resolution anoscopy.

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