scholarly journals Serological and Molecular Surveillance of Infectious Bronchitis Virus Infection in Free-Range Chickens and Guinea Fowls in the Ga-East District of Ghana

2018 ◽  
Vol 2018 ◽  
pp. 1-6
Author(s):  
Matilda Ayim-Akonor ◽  
Doreen Dela Owusu-Ntumy ◽  
Hilda Emefa Ohene-Asa ◽  
Agyekum Oduro-Abrokwa ◽  
Patricia Hammond ◽  
...  
Keyword(s):  
Infectious Bronchitis Virus ◽  
Infectious Bronchitis ◽  
Free Range ◽  
Glycoprotein Gene ◽  
Worldwide Distribution ◽  
Test Kit ◽  
Commercial Chicken ◽  
Infectious Bronchitis Virus Infection ◽  
History Of ◽  
Local Chickens

Infectious bronchitis is an economically important disease with worldwide distribution. Information is available on the presence of infectious bronchitis virus in commercial chicken in parts of Ghana but there is no information on free-range poultry and guinea fowls in the country. Possible IBV infections among free-range chickens and guinea fowls in Abokobi and Frafraha communities in the Ga-East district of the Greater Accra Region of Ghana were investigated using serology and PCR. Blood, tracheal, and cloacal swabs were obtained from 219 free-range chickens and guinea fowls with no respiratory symptoms and no history of IBV vaccination. Sera were evaluated for IBV antibodies by ELISA using commercial IBV test kit from IDEXX, Inc., USA. Swab samples were evaluated for S1 glycoprotein gene by one-step RT PCR. All the swab samples tested negative for IBV. 16% of all tested sera were positive for IBV. IBV seroprevalence in guinea fowls was 0%. 21.2% of sera from local chickens were positive for IBV. The seroprevalence of IBV among local chickens from Frafraha was 30% and that of Abokobi was 7.7%. This study shows exposure of local chickens in the study communities to IBV.

scholarly journals Infectious bronchitis virus from chickens in Al-Hasa, Saudi Arabia 2015-2016

2019 ◽  
Vol 12 (3) ◽  
pp. 424-433 ◽  
Author(s):  
Musaed Abdulaziz Alsultan ◽  
Mohamed Ali Alhammadi ◽  
Maged Gomaa Hemida
Keyword(s):  
Infectious Bronchitis Virus ◽  
Phylogenetic Trees ◽  
Molecular Characteristics ◽  
Gene Sequences ◽  
S1 Gene ◽  
Infectious Bronchitis ◽  
Chicken Population ◽  
Commercial Chicken ◽  
Tissue Specimens ◽  
High Degree

Aim: This study aimed to isolate some of the currently circulating infectious bronchitis virus (IBV) strains from some broiler chicken farms in Al-Hasa and to do some molecular characteristics of these strains. Materials and Methods: We collected 300 tissue specimens, including the trachea, bronchi, lungs, and kidneys from some four commercial chicken farms showing respiratory manifestations. We tested these tissue specimens by the real-time polymerase chain reaction (RT-PCR) and gel-based PCR. We selected some PCR positive samples for isolation in the embryonated chicken eggs (ECE). We sequenced some PCR-positive samples and conducted phylogenetic analysis based on the obtained sequences. Results: Our molecular surveillance revealed that 31.6% of the tested specimens were IBV positive by PCR. We selected some positive specimens showing low Ct values by the qRT-PCR for virus isolation by the ECE. The infected eggs showed hemorrhage, dwarfing, and death in some cases after three passages in the ECE. We sequenced some of the positive PCR specimens and used the obtained sequences to draw the phylogenetic tree based on the partial IBV-ORF-1a, N, and S1 gene sequences. The phylogenetic trees based on the IBV-N and S1 gene sequences showed that the circulating IBV strains in Al-Hasa during 2016 was showing a high degree of identity to some strains from Taiwan and Italy. Meanwhile, the grouping of these strains based on the IBV-S1 sequences revealed that the currently circulating IBV strains in Al-Hasa belonged to Gr.I.7 along with strains from Taiwan. Conclusion: Our results confirmed the continuous circulation of the IBV among the chicken population in Al-Hasa despite the intensive application of vaccines against this virus.

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