scholarly journals Hypoglycaemic and Antioxidant Effects of Propolis of Chihuahua in a Model of Experimental Diabetes

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Nelly Rivera-Yañez ◽  
Mario Rodriguez-Canales ◽  
Oscar Nieto-Yañez ◽  
Manuel Jimenez-Estrada ◽  
Maximiliano Ibarra-Barajas ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Plasma Insulin ◽  
Experimental Diabetes ◽  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Diabetic Mice ◽  
Plasma Insulin Levels ◽  
Streptozotocin Induced Diabetes ◽  
Ethanolic Extract Of Propolis ◽  
Antioxidant Effects

Propolis is a bee-collected natural product that has been proven to have various bioactivities. This study tested the effects of a Mexican propolis on streptozotocin-induced diabetes mellitus in a murine model. The results showed that an ethanolic extract of propolis of Chihuahua (EEPCh) significantly inhibited increases in blood glucose and the loss of body weight in diabetic mice. EEPCh increased plasma insulin levels in STZ-diabetic mice, whereas, in untreated diabetic mice, there was no detection of insulin. EEPCh had a high antioxidant capacity (SA50 = 15.75 μg/mL), which was directly related to the concentrations of total phenols (314 mg GAE/g of extract) and flavonoids (6.25 mg QE/g of extract). In addition, increased activities of the enzymes superoxide dismutase, catalase, and glutathione peroxidase were observed in diabetic mice treated with EEPCh. Compounds such as pinocembrin, quercetin, naringin, naringenin, kaempferol, acacetin, luteolin, and chrysin were identified by HPLC-MS analysis. This investigation demonstrated that propolis of Chihuahua possesses hypoglycaemic and antioxidant activities and can alleviate symptoms of diabetes mellitus in mice. These effects may be directly related to the chemical composition of propolis, as most of the compounds identified in propolis are reportedly active in terms of the different parameters evaluated in this work.

scholarly journals Inhibition study of proinflammatory factor with ethanolic extract of propolis on innate immunity from diabetes mellitus mice model

2018 ◽  
Vol 90 (2) ◽  
Author(s):  
Bambang Pristiwanto ◽  
Sutiman B. Sumitro ◽  
Muhammad S. Djati ◽  
Aris Soewondo ◽  
Hideo Tsuboi ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Innate Immunity ◽  
Body Weight ◽  
Metabolic Diseases ◽  
Ethanolic Extract ◽  
Control Group ◽  
Mice Model ◽  
Innate Immunity Cells ◽  
Proinflammatory Factor ◽  
Ethanolic Extract Of Propolis

Health becomes an important topic today. One current problem was how to treat the effects of metabolic diseases, such as diabetes mellitus (DM). Thus, this study used an ethanolic extract of propolis (EEP), to test their ability as the supplement in the diabetes treatment to reduce inflammation, through proinflammatory factor response, especially nuclear factor κB (NF-κB). The streptozotocin- induced diabetes mellitus (SID) mice model was used, and expression of an proinflammatory factor was analyzed in their innate immunity cells with 3 doses of EEP, i.e. 50 mg/kg body weight, 100 mg/kg body weight, and 200 mg/kg body weight. Treatment of EEP in SID with three doses treatment decrease the number of macrophages with NF-κB expression significantly with DM control group. The results of B cells with NF-κB expression showed that EEP treatment in SID could decrease in dose 1 and dose 3, but not in dose 2. Proinflammatory cytokines expression of macrophage, especially Tumor Necrosis Factor-α and Interferon-γ, with EEP treatment in SID could decrease in three doses. This study suggests that EEP could reduce inflammation by inhibiting the development of NF-κB in innate immunity cells.

scholarly journals Chemical Constituents from the Aerial Parts of Gynura Bicolor

2012 ◽  
Vol 7 (12) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Jian Chen ◽  
Sven Mangelinckx ◽  
An Adams ◽  
Wei-lin Li ◽  
Zheng-tao Wang ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Chemical Constituents ◽  
Pharmacological Study ◽  
Ethanolic Extract ◽  
Diabetic Mice ◽  
Aerial Parts ◽  
Treatment Of Diabetes ◽  
Aqueous Ethanolic Extract ◽  
Gynura Bicolor ◽  
First Time

Gynura bicolor (Willd.) DC., is used in folk recipes for the treatment of diabetes mellitus in Jiangsu, Zhejiang and Sichuan province in the south of China. A previous pharmacological study proved that the plant showed significant hypoglycemic activity on normal and alloxan-diabetic mice. In this study, two terpenes, four megastigmane-type norisoprenoids and two glycosides were isolated from the aqueous ethanolic extract of the aerial parts of Gynura bicolor and characterized mainly by NMR spectroscopy and mass spectrometry. These compounds were isolated for the first time from this plant, and no evidence could be found for the previous reported presence of megastigmane-type norisoprenoids in the genus Gynura.

EFFECT OF AMINOGUANIDINE ON CATARACTOGENESIS IN EXPERIMENTAL DIABETES MELLITUS

2019 ◽  
Vol 25 (5-6) ◽  
pp. 303-308
Author(s):  
A. A Spasov ◽  
Yulia A. Govorova ◽  
L. V Naumehko ◽  
D. A Babkov ◽  
A. S Taran ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Experimental Diabetes ◽  
Laboratory Animals ◽  
Cataract Formation ◽  
Advanced Glycation ◽  
Experimental Diabetes Mellitus ◽  
Nonenzymatic Glycosylation ◽  
Glycation End Products ◽  
End Products ◽  
Streptozotocin Induced Diabetes

Nonenzymatic glycosylation of lens proteins in diabetes mellitus is one of the pathogenetic mechanisms of cataract formation. According to the results of this study, aminoguanidine, which has anti-glycation activity, inhibits cataractogenesis in experimental diabetes. Laboratory animals with streptozotocin-induced diabetes mellitus treated with aminoguanidine showed less clouding in the lenses, and the content of advanced glycation end products, in particular, carboxymethyllysine, in the lenses was found to be reduced compared to the same parameters in animals from the control diabetic group.

scholarly journals Anti-Diabetic Potential of the Leaves of Anisomeles malabarica in Streptozotocin Induced Diabetic Rats

2017 ◽  
Vol 43 (4) ◽  
pp. 1689-1702 ◽  
Author(s):  
Peddanna Kotha ◽  
Kameswara Rao Badri ◽  
Ramya Nagalapuram ◽  
Rajasekhar Allagadda ◽  
Appa Rao Chippada
Keyword(s):  
Diabetes Mellitus ◽  
Glucose Tolerance ◽  
Ethyl Acetate ◽  
Plasma Insulin ◽  
Ethyl Acetate Extract ◽  
Glycosylated Hemoglobin ◽  
Diabetic Rats ◽  
Active Fraction ◽  
Insulin Levels ◽  
Plasma Insulin Levels

Background/Aims: Diabetes mellitus is a pandemic metabolic disorder that is affecting a majority of populations in recent years. There is a requirement for new drugs that are safer and cheaper due to the side effects associated with the available medications. Methods: We investigated the anti-diabetic activity of leaves of Anisomeles malabarica following bioactivity guided fractionation. The different solvent (hexane, ethyl acetate, methanol and water) extracts of A. malabarica leaves were used in acute treatment studies to evaluate and identify the active fraction. The ethyl acetate extract was subjected to further fractionation using silica gel column chromatography and the compounds were identified by LC-SRM/MS and GC-MS. Additional chronic treatment studies were carried out using this active fraction (AMAF) for 30 days in experimental diabetic rats. Fasting blood glucose (FBG), glycosylated hemoglobin (HbA1c), plasma insulin levels and glucose tolerance were measured along with insulin resistance/sensitivity indicators (HOMA-IR, HOMA-β and QUICKI) to assess the beneficial effects of A. malabarica in the management of diabetes mellitus. Results: Among the different solvent extracts tested, ethyl acetate extract showed maximum (66%) anti-hyperglycemic activity. The hexane and ethyl acetate (1: 1) fraction that has maximum anti-diabetic activity was identified as active fraction of A. malabarica (AMAF). The FBG, HbA1c, plasma insulin levels and insulin sensitivity/resistance indicators such as glucose tolerance, HOMA-IR, HOMA-β and QUICKI were significantly improved to near normal in diabetic rats treated with AMAF. Further, we identified key flavonoids and fatty acids as the anti-diabetic active principles from the AMAF of A. malabarica leaves. Conclusion: The results of our study suggest that Anisomeles malabarica has potential anti-diabetic activity in STZ induced diabetic rats.

scholarly journals HISTOLOGICAL AND MORPHOMETRIC ASPECTS OF STRUCTURE OF AORTIC WALL AND ITS HEMOMICROCIRCULATORY BED IN LATE STAGES OF EXPERIMENTAL DIABETES MELLITUS

2021 ◽  
Vol 21 (3) ◽  
pp. 222-228
Author(s):  
M.N. Tsitovskyi ◽  
M.V. Logash ◽  
I.I. Savka
Keyword(s):  
Diabetes Mellitus ◽  
Cardiovascular Diseases ◽  
Aortic Wall ◽  
Social Issues ◽  
Experimental Diabetes ◽  
White Male ◽  
Male Rats ◽  
World Health ◽  
Significant Difference ◽  
Streptozotocin Induced Diabetes

According to the World Health Organization, cardiovascular diseases and diabetes mellitus occupy a significant niche in the structure of diseases with high disability and mortality impact and pose major healthcare and social issues. It should be stressed that 50-80% of fatal cases in patients with diabetes are associated with cardiovascular diseases. The purpose of this study is to determine the histostructural characteristics and to perform morphometric analysis of the components of the aortic wall and its hemomicrocirculatory bed of the aorta in 6 and 8 weeks of streptozotocin-induced diabetes mellitus. The material for the histological study included the sections of the wall of the ascending aorta, the aortic arch, and the descending aorta taken from 26 sexually mature white male rats weighing 100 - 160 g. For morphometric examination, a series of photos of the aortic wall was taken using a Meiji MT4300 LED microscope with an x40 objective, x10 ocular. The measurements were carried out using the Image J software. The development of micro - and macroangiopathies in experimental animals with 8-week streptozotocin-induced diabetes mellitus was histologically confirmed. Statistical analysis revealed a significant difference in all morphometric parameters of the components of the aortic wall and the vessels of its hemomicrocirculatory bed after 8 weeks of experimental diabetes as compared with the normal values, control values, and in values obtained at the 6-week period of the experiment. The study has demonstrated clear dependence between the severity of destructive changes in the aortic wall and sections of its hemomicrocirculatory bed and the duration of the experiment.

scholarly journals Therapeutic effects of ethanolic extract from the green cocoon shell of silkworm Bombyx mori on type 2 diabetic mice and its hypoglycaemic mechanism

2019 ◽  
Vol 8 (3) ◽  
pp. 407-420 ◽  
Author(s):  
Jin-Ge Zhao ◽  
Hai-Yan Wang ◽  
Zheng-Guo Wei ◽  
Yu-Qing Zhang
Keyword(s):  
Diabetes Mellitus ◽  
Bombyx Mori ◽  
Protein Metabolism ◽  
Complex Disease ◽  
Ethanolic Extract ◽  
Therapeutic Effects ◽  
Diabetic Mice ◽  
Type 2 Diabetic ◽  
Silkworm Bombyx Mori

Diabetes mellitus is a clinically complex disease characterized by hyperglycaemia with disturbances in carbohydrate, fat and protein metabolism.

scholarly journals Alternanthera bicolor Produces Hypoglycemic Effect in Alloxan-Induced Diabetic Mice through its Antioxidant Activity

2019 ◽  
Vol 18 (1) ◽  
pp. 49-60
Author(s):  
Kousik Ahmed Khan ◽  
Manik Zahan ◽  
Fatema Zohura Talukder ◽  
Riaz Uddin ◽  
Manik Chandra Shill ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Hypoglycemic Effect ◽  
Lipid Peroxidation Product ◽  
Diabetic Mice ◽  
Dpph Radical

The current investigation was carried out to evaluate the antioxidant properties of ethanolic extract of Alternanthera bicolor and to assess the potential hypoglycemic effect of the extract in alloxan-induced diabetic mice. HPLC-DAD method was used to determine polyphenolic compounds present in the extract. Different in vitro assays (i.e. DPPH radical scavenging activity test, reducing power test, NO radical inhibition assay and scavenging of hydrogen peroxide) were used to determine the antioxidant potential of the plant. Antidiabetic activity was evaluated in alloxan-induced diabetic mice by glucose tolerance test and standard biochemical analyses. HPLC-DAD analysis of the extract confirmed the presence of (+)-catechin hydrate, caffeic acid, quercetin and kaempferol. A. bicolor showed potent antioxidant activities in DPPH radical, hydrogen peroxide and nitric oxide scavenging assays. Moreover, A. bicolor showed potent reducing power and dose-dependent increment of total antioxidant capacity. Furthermore, the plant showed potent hypoglycemic activities in alloxan-induced diabetic mice. Ethanolic extract of the plant at doses of 200- and 400-mg/kg body weight (administered orally for 3 weeks) significantly decreased the elevated levels of blood glucose, lipid peroxidation product TBARS, hydroperoxides and nitric oxide in experimental animals. Apart from these activities, the ethanolic extract of the plant restored the reduced catalase function in liver. From this study, we can conclude that ethanolic extract of A. bicolor exhibited hypoglycemic and antioxidant activities in alloxan-induced diabetic mice. Dhaka Univ. J. Pharm. Sci. 18(1): 49-60, 2019 (June)

scholarly journals Morphological Characteristics of Changes in the Duodenal Wall Within 14-56 Days of the Development of Streptozotocin-Induced Experimental Diabetes Mellitus

2020 ◽  
Vol 27 (4) ◽  
pp. E2020413
Author(s):  
Ihor Bilinskyi
Keyword(s):  
Diabetes Mellitus ◽  
Lamina Propria ◽  
Experimental Diabetes ◽  
Histological Study ◽  
Duodenal Mucosa ◽  
Male Rats ◽  
Duodenal Wall ◽  
Apical Surface ◽  
Experimental Diabetes Mellitus ◽  
Streptozotocin Induced Diabetes

The objective of the research was to determine the morphological features of the duodenal wall of animals within 14-56 days of developing streptozotocin-induced diabetes mellitus using light optical microscopy. Materials and Methods. The research was carried out on 40 white nonlinear adult male rats. Diabetes mellitus was simulated by a single intraperitoneal injection of streptozotocin (Sigma, USA) at a dose of 60 mg/kg body weight. The material was taken from the duodenum on the 14th, 28th and the 56th days after the onset of experimental diabetes mellitus. For histological study, the preparations were made using the conventional method, which included the staining of sections with hematoxylin and eosin. Results. Streptozotocin-induced diabetes mellitus was experimentally found to lead to dystrophic changes in the epithelial components of the duodenal mucosa from the 14th day of developing. There were observed a shortening of the villi of the mucous membrane and a lack of distinctness of striated border contours on the apical surface of epitheliocytes. Between the connective-tissue fibers of the lamina propria of the mucosa and thin-walled vessels, the cellular elements, including mainly macrophages, lymphocytes, were found. There was a shortening of the villi, edema and histiolymphocytic infiltration of the villous stroma 28 days after developing experimental diabetes mellitus. The epithelium covering was discontinuous; numerous areas of desquamation were found at the apex of the villi. Fifty-six days after developing experimental diabetes mellitus, the destruction and desquamation of the epithelium of the villi and crypts were observed. The surface of the duodenal mucosa smoothed down due to the shortening and flattening of the villi (indicating their atrophy), while the crypts elongated and their depth increased. Conclusions. Histological study of the duodenal wall of diabetic animals showed pronounced desquamation at the apex of the villi, destructive and dystrophic changes in the surface epithelium, edema and increased cellular infiltration of the lamina propria of the mucosa. Thus, in diabetes mellitus, structural changes in the duodenal wall of rats are characterized by the dystrophic processes, which can be considered as the morphological reflection of enteropathy.

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