scholarly journals The Effect of Irradiation with a 405 nm Blue-Violet Laser on the Bacterial Adhesion on the Osteosynthetic Biomaterials

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Chika Terada ◽  
Takahiro Imamura ◽  
Tomoko Ohshima ◽  
Nobuko Maeda ◽  
Seiko Tatehara ◽  
...  
Keyword(s):  
Bacterial Adhesion ◽  
Postoperative Infection ◽  
Major Complication ◽  
Bactericidal Effect ◽  
Alamar Blue ◽  
Effective Prevention ◽  
Material Surfaces ◽  
Significant Difference ◽  
Redox Indicator ◽  
Colony Forming Assay

Delayed postoperative infection is known as a major complication after bone surgeries using osteosynthetic biomaterial such as titanium (Ti) and bioresorbable organic materials. However, the precise cause of this type of infection is still unclear and no effective prevention has been established. The purpose of this study is to investigate the effect of irradiation with a 405 nm blue-violet laser on the bacteria adhered on the Ti and hydroxyapatite-poly-L-lactic acid- (HA-PLLA) based material surfaces and to verify the possibility of its clinical application to prevent the delayed postoperative infection after bone surgeries using osteosynthetic biomaterial. The suspension of Staphylococcus aureus FDA 209P was delivered onto the surface of disks composed of Ti or HA-PLLA. Bacterial adhesion on each disk was observed using a scanning electron microscope (SEM). After thorough washing with distilled water, the growth of bacteria attached to the material surfaces was examined with an alamar blue-based redox indicator. Moreover, a bactericidal effect of 405 nm blue-violet laser irradiation on residual bacteria on both materials was investigated using colony-forming assay. As a result, there was no significant difference in the bacterial adhesion between Ti and HA-PLLA materials. In contrast, 45 J/cm2 of irradiation with 405 nm blue-violet laser inhibited the bacterial growth at approximately 93% on Ti disks and at approximately 99% on HA-PLLA disks. This study clearly demonstrated the possibility that the irradiation with a 405 nm blue-violet laser is useful as an alternative management strategy for the prevention of delayed postoperative infection after bone surgeries using osteosynthetic biomaterials.

scholarly journals High Bactericidal Self-Assembled Nano-Monolayer of Silver Sulfadiazine on Hydroxylated Material Surfaces

Materials ◽  
2019 ◽  
Vol 12 (17) ◽  
pp. 2761 ◽  
Author(s):  
Angelo Taglietti ◽  
Giacomo Dacarro ◽  
Daniele Barbieri ◽  
Lucia Cucca ◽  
Pietro Grisoli ◽  
...  
Keyword(s):  
Bacterial Adhesion ◽  
High Surface ◽  
Bactericidal Effect ◽  
Silver Sulfadiazine ◽  
Layer By Layer ◽  
Self Assembled Monolayer ◽  
Surface Mass ◽  
Material Surfaces ◽  
Functionalized Materials ◽  
Self Assembled

Anti-infective surfaces are a modern strategy to address the issue of infection related to the clinical use of materials for implants and medical devices. Nanocoatings, with their high surface/mass ratio, lend themselves to being mono-layered on the material surfaces to release antibacterial molecules and prevent bacterial adhesion. Here, a “layer-by-layer” (LbL) approach to achieve a self-assembled monolayer (SAM) with high microbicidal effect on hydroxylated surfaces is presented, exploiting the reaction between a monolayer of thiolic functions on glass/quartz surfaces and a newly synthesized derivative of the well-known antibacterial compound silver sulfadiazine. Using several different techniques, it is demonstrated that a nano-monolayer of silver sulfadiazine is formed on the surfaces. The surface-functionalized materials showed efficient bactericidal effect against both Gram-positive and Gram-negative bacteria. Interestingly, bactericidal self-assembled nano-monolayers of silver sulfadiazine could be achieved on a large variety of materials by simply pre-depositing glass-like SiO2 films on their surfaces.

Effect of surface sealant on surface roughness and bacterial adhesion of bulk-fill composites

2021 ◽  
pp. 096739112110055
Author(s):  
Gunce Ozan ◽  
Meltem Mert Eren ◽  
Cansu Vatansever ◽  
Ugur Erdemir
Keyword(s):  
Surface Roughness ◽  
Bacterial Adhesion ◽  
Confocal Laser ◽  
High Caries Risk ◽  
Two Samples ◽  
Significant Difference ◽  
Restorative Materials ◽  
Surface Sealants ◽  
Surface Sealant ◽  
Effect Of Surface

Surface sealants are reported to ensure surface smoothness and improve the surface quality of composite restorations. These sealants should also reduce the bacterial adhesion on composite surfaces however, there is not much information regarding their performance on bulk-fill composite materials. The aim of this study was to evaluate the effect of surface sealant application on surface roughness and bacterial adhesion of various restorative materials. Disc-shaped samples were prepared from a compomer, a conventional composite and three bulk-fill composites. Specimens of each group were divided into two groups (n = 9): with/without surface sealant (Biscover LV, [BLV]). Surface roughness values were examined by profilometry and two samples of each group were examined for bacterial adhesion on a confocal laser scanning microscope (CLSM). Bacterial counts were calculated by both broth cultivation and microscopic images. Results were analyzed with one-way ANOVA and Bonferroni/Dunn tests. Following the BLV application, there was a decrease in the surface roughness values of all groups however, only Tetric N-Ceram Bulk and Beautifil-Bulk groups showed significantly smoother surfaces (p < 0.001). There were no significant differences among material groups without BLV application. Evaluating bacterial adhesion after BLV application, conventional composite had the lowest values among all followed by the compomer group. Beautifil-Bulk had significantly the highest bacterial adhesion (p < 0.05), followed by Tetric N-Ceram Bulk group. Without BLV application, there was no significant difference among bacterial adhesion values of groups (p > 0.05). CLSM images showed cell viability in groups. Bulk-fill composites showed higher bacterial adhesion than conventional composite and compomer materials. The surface sealant was found to be highly effective in lowering bacterial adhesion, but not so superior in smoothing the surfaces of restorative materials. So, surface sealants could be used on the restorations of patients with high caries risk.

scholarly journals Absence of Association between Preoperative Estimated Glomerular Filtration Rates and Postoperative Outcomes following Elective Gastrointestinal Surgeries: A Prospective Cohort Study

2018 ◽  
Vol 2018 ◽  
pp. 1-7
Author(s):  
Sivesh K. Kamarajah ◽  
Behrad Barmayehvar ◽  
Mustafa Sowida ◽  
Amirul Adlan ◽  
Christina Reihill ◽  
...  
Keyword(s):  
Risk Stratification ◽  
Glomerular Filtration ◽  
Elective Surgery ◽  
Gastrointestinal Surgery ◽  
Major Complication ◽  
Postoperative Outcomes ◽  
Queen Elizabeth Hospital ◽  
Complication Rates ◽  
Significant Difference ◽  
The Impact

Background. Preoperative risk stratification and optimising care of patients undergoing elective surgery are important to reduce the risk of postoperative outcomes. Renal dysfunction is becoming increasingly prevalent, but its impact on patients undergoing elective gastrointestinal surgery is unknown although much evidence is available for cardiac surgery. This study aimed to investigate the impact of preoperative estimated glomerular filtration rate (eGFR) and postoperative outcomes in patients undergoing elective gastrointestinal surgeries. Methods. This prospective study included consecutive adult patients undergoing elective gastrointestinal surgeries attending preassessment screening (PAS) clinics at the Queen Elizabeth Hospital Birmingham (QEHB) between July and August 2016. Primary outcome measure was 30-day overall complication rates and secondary outcomes were grade of complications, 30-day readmission rates, and postoperative care setting. Results. This study included 370 patients, of which 11% (41/370) had eGFR of <60 ml/min/1.73 m2. Patients with eGFR < 60 ml/min/1.73 m2 were more likely to have ASA grade 3/4 (p<0.001) and >2 comorbidities (p<0.001). Overall complication rates were 15% (54/370), with no significant difference in overall (p=0.644) and major complication rates (p=0.831) between both groups. In adjusted models, only surgery grade was predictive of overall complications. Preoperative eGFR did not impact on overall complications (HR: 0.89, 95% CI: 0.45–1.54; p=0.2). Conclusions. Preoperative eGFR does not appear to impact on postoperative complications in patients undergoing elective gastrointestinal surgeries, even when stratified by surgery grade. These findings will help preassessment clinics in risk stratification and optimisation of perioperative care of patients.

Impact of Physical Chemical Characteristics of Abutment Implant Surfaces on Bacteria Adhesion

2016 ◽  
Vol 42 (2) ◽  
pp. 153-158 ◽  
Author(s):  
Erica Dorigatti de Avila ◽  
Rafael Scaf de Molon ◽  
Bruno P. Lima ◽  
Renate Lux ◽  
Wenyuan Shi ◽  
...  
Keyword(s):  
Bacterial Adhesion ◽  
Electrostatic Interactions ◽  
Chemical Elements ◽  
Human Tooth ◽  
Chemical Characteristics ◽  
Material Surfaces ◽  
Initial Adhesion ◽  
Implant Abutment ◽  
Adhesion Phase ◽  
Bacteria Adhesion

Surface attachment is the first step in biofilm formation, and the ability of bacteria to adhere to surfaces and develop a biofilm is directly influenced by electrostatic interactions between the bacteria and the chemical composition of material surfaces. Here, we investigated the influence of physical and chemical characteristics of titanium (Ti) and zirconia (ZrO2) as implant abutment surfaces on the bacterial adhesion phase and compared the results to bovine enamel (BE) simulating a human tooth. To achieve this goal, we used 2 common pathogens of the oral cavity, Streptococcus mutans UA140 and Porphyromonas gingivalis 33277. To investigate the influence of material surfaces on bacterial adhesion, we studied the surface free energy as well as the topography by atomic force microscopy, and the chemical elements composition by scanning electron microscopy equipped with an energy dispersive X-ray spectroscope. Our results indicated a hydrophobic characteristic for all of the materials; however, the presence of polar and nonpolar components could aid in understanding why greater numbers of bacteria had adhered to BE compared to the other surfaces. Our confocal microscopy data support the proposition that electrostatic interactions, indeed, affected the initial adhesion phase. Within the limitations of a laboratory study, the results revealed bacterial adhered on BE and no bacteria could be observed by confocal images on Ti and ZrO2 implant abutment surfaces.

scholarly journals Functional Outcome of Intramedullary Nailing with Rush Pin for Pediatric Both Bone Fractures of Forearm in Western Hilly Nepal

2017 ◽  
Vol 5 (1) ◽  
pp. 23
Author(s):  
Rahul Shrestha ◽  
Subin Byanjankar ◽  
Rajeev Dwivedi ◽  
Ruban Raj Joshi ◽  
Mahesh Raj Ghimire
Keyword(s):  
Functional Outcome ◽  
Intramedullary Nailing ◽  
Bone Fractures ◽  
Low Cost ◽  
Major Complication ◽  
Western World ◽  
Chi Square ◽  
Significant Difference ◽  
Elastic Nails ◽  
Mean Time

Introduction: Both bone diaphysis fracture of forearm is common in children of hilly area in Nepal because the children climbs tree and cliff for playing and cutting grass. Close reduction and casting is the preferred method of treatment these fractures but the chances of re-displacement is very high. Intramedullary nailing with titanium elastic nails or rush pins is widely accepted these days with good outcome. Titanium nails are popular in western world but is costly whereas rush pins are cheap and are preferred in developing world. The aim of this study was to evaluate the functional outcome of intramedullary rush pin for pediatric both bone fracture of forearm. Methods: In this retrospective, observational study done from 1st of February 2017 to 31st of March 2017, a total of sixty patients with both bone fractures of forearm were treated with intramedullary rush pin and followed up for six months for evaluation of functional outcome. T-test and Chi-square tests were done. Results: Closed reduction and internal fixation was done in 48 (80%) patients. Mean age of the patients was 9.23 year (SD=2.77). Fifty-six (93.3%) patients were male with a significant difference (p<0.001). With price et al. grading system, 49 (81.7%) patients had excellent results, nine (15%) had good and two (3.3%) patients had fair results. There was no major complication. Mean time to implant removal was 24.16 weeks (SD=1.62) from the time of surgery with range of 20 to 28 weeks. Among transverse and oblique fractures, patients with transverse fracture were likely to have better outcome (p=0.04). Conclusion: Intramedullary nailing with rush pin gives excellent to good functional outcome in majority of the cases without major complications at low cost for management of both bone fractures of forearm in children.

scholarly journals Carbonated Hydroxyapatite Containing Propolis as an Antibacterial Agent Candidate against Aggregatibacter actinomycetemcomitans

2020 ◽  
Vol 25 (3) ◽  
Author(s):  
Ayunda Nur Sukmawati ◽  
Sri Pramestri ◽  
Al. Sri Koes Soesilowati ◽  
Suryono Suryono
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Adhesion ◽  
Antibacterial Agent ◽  
Alveolar Bone ◽  
Inhibition Zone ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Test Method ◽  
Graft Material ◽  
Carbonated Hydroxyapatite ◽  
Significant Difference

One of the periodontal pathogenic bacteria that can cause periodontitis and alveolar bone destruction is Aggregatibacter actinomycetemcomitans. An alveolar bone defect can be treated using a bone graft. Carbonated hydroxyapatite (CHA) is an alloplastic graft material. Alloplastic materials do not have vascularization, which will increase the risk of bacterial adhesion. Therefore, adding an antibacterial agent is needed to prevent bacterial adhesion, which will improve periodontal healing. Propolis is a natural ingredient that has broad-spectrum antibacterial activity and does not cause bacterial resistance. This study aimed to assess the antibacterial activity of carbonated hydroxyapatite after being incorporated with propolis against Aggregatibacter actinomycetemcomitans. Carbonated hydroxyapatite was embedded into four different concentrations of propolis solution (2.5%, 5%, 7.5%, and 10%). An antimicrobial assay against Aggregatibacter actinomycetemcomitans was done using the disc diffusion test method. The inhibition zone was measured to determine the antibacterial ability of the specimens. The inhibition zone was found on the carbonated hydroxyapatite incorporated with propolis at all concentrations. Carbonated hydroxyapatite incorporated with 10% propolis showed the largest inhibition zone. Data analysis using the Kruskal–Wallis test showed a significant difference between the groups tested (p <.05). In conclusion, carbonated hydroxyapatite incorporated with propolis has antibacterial activity against Aggregatibacter actinomycetemcomitans

The Prevalence of Injuries among Pianists in Music Schools in Ireland

2000 ◽  
Vol 15 (4) ◽  
pp. 155-160
Author(s):  
Nora Shields ◽  
Sara Dockrell
Keyword(s):  
Risk Factors ◽  
Prevention Programs ◽  
Predisposing Factors ◽  
Lifetime Prevalence ◽  
Music Schools ◽  
Effective Prevention ◽  
Common Location ◽  
Significant Difference ◽  
Piano Playing

The purpose of this study was to determine the prevalence of injuries among pianists in music schools in Ireland. The main objectives of the study were to investigate the anatomical locations of these injuries, the most commonly reported symptoms, and possible risk factors for the development of playing-related injuries. A hand-delivered questionnaire was distributed to 182 students from seven participating music schools. The lifetime prevalence for a playing-related injury, which prevented piano playing for a period of 48 hours or longer, was 25.8%. The wrist was the most common location of injury (36.6%). Pain was the most commonly reported symptom among the respondents. No statistically significant difference in the rate of injury was found between the genders. It was concluded that further research is required into the prevalence of injury and to the predisposing factors of injury so that effective prevention programs can be put in place.

scholarly journals Audit of Complications in an Otolaryngology Led Skull-Base Surgical Practice

2018 ◽  
Vol 80 (06) ◽  
pp. 586-592
Author(s):  
Uma Patnaik ◽  
Smriti Panda ◽  
Alok Thakar
Keyword(s):  
Skull Base ◽  
Surgical Complications ◽  
Skull Base Surgery ◽  
Major Complication ◽  
Anterior Skull Base ◽  
Complication Rates ◽  
Permanent Disability ◽  
Lateral Skull Base ◽  
Significant Difference ◽  
Surgical Complexity

Objective This study was aimed to classify and study complications of surgery of the cranial base, primarily from an otorhinolaryngology perspective. Design This study was designed with consecutive cohort of skull base surgical cases. Setting Tertiary referral academic center. Participants Patients having skull-base surgery at a otorhinolaryngology based skull-base unit, from 2002 to 2015. Main Outcome Measures Enumeration of complications is the main outcome of this study. Surgical procedures, categorized for complexity as per a unified system, are applicable to endoscopic and open procedures. Complications were categorized as per the British Association of Otolaryngologists coding of surgical complications. Complication classified as major if life-threatening, causing permanent disability, or compromising the result of surgery. Results A total of 342 patients (n = 342) were operated; 13 patients' records were excluded due to < 6 months posttreatment follow-up. The study group constituted 204 anterior skull-base (endoscopic, 120; open/external, 84) and 125 lateral skull-base procedures. Complication rates noted to increase in both groups with increasing complexity of surgical intervention. Anterior skull-base surgery (total complications, 11%; major, 3%; death, 0.5%) noted to have significantly less surgical complications than lateral skull-base surgery (total complications, 33%; major, 15%; death, 1.6%; p < 0.001). Among the anterior procedures no significant difference noted among endoscopic and external approaches when compared across similar surgical complexity. Conclusion Despite improvement in surgical and perioperative care, the overall major complication rate in a contemporary otolaryngology led, primarily extradural, skull-base practice is noted at 8%. Perioperative mortality, though rare, was encountered in 1%. A standard method for categorization of surgical complexity and the grade of complications as reported here is recommended.

scholarly journals Association between semen quality among men with different occupational exposures and risk of recurrent spontaneous abortion in island residents

2019 ◽  
Vol 02 (02) ◽  
pp. 49-57
Author(s):  
Wenli Cao ◽  
Xinyao Pan ◽  
Feijun Ye ◽  
Jing Zhou ◽  
Zengshu Huang ◽  
...  
Keyword(s):  
Spontaneous Abortion ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Occupational Exposures ◽  
Recurrent Spontaneous Abortion ◽  
Semen Parameters ◽  
Control Group ◽  
Effective Prevention ◽  
Mechanistic Investigation ◽  
Significant Difference

Objective: This study aimed to evaluate the semen quality of male adults whose spouses suffer from recurrent spontaneous abortion (RSA) in the island area, and to explore the association between RSA and occupational exposures. Methods: A total of 131 male patients were recruited and divided into two groups: spouse with recurrent spontaneous abortion group (RSA group, [Formula: see text]) and the normal fertility group (control group, [Formula: see text]). Information such as height, weight and occupational exposure history of 131 men were obtained. Semen samples were collected and analyzed. Differences in semen parameters and DNA fragmentation index (DFI) between the two groups were compared. Odds ratios (ORs) and their corresponding 95% confidence intervals were calculated to evaluate the association between occupational exposures and RSA status. Results: Overall, no significant difference was found in sperm concentration, progressive motility and normal morphology rate between RSA and control groups. Only DFI was observed to be significantly higher in the RSA group. Evaluation of the receiver operating characteristic (ROC) curve showed DFI (AUC: 0.623, [Formula: see text]) could discriminate between males from the couples with RSA and without RSA. Conclusion: The analysis of conventional semen parameters could not directly reflect their influence on embryonic development, and test of integrity of the sperm DNA is of paramount importance to fully understand male fertility. It is crucial to conduct studies regarding occupational exposures and pregnancy loss and/or RSA risk, since it will provide population-level data to aid in the identification of important risk factors that warrant further mechanistic investigation, and eventually lead to effective prevention and treatment strategies.

scholarly journals Stent or Surgery for Malignant Low Bileduct Obstruction?

HPB Surgery ◽  
1997 ◽  
Vol 10 (3) ◽  
pp. 179-181
Author(s):  
Ingemar Ihse ◽  
Lars Hansson ◽  
Lars-Erik Hammarström ◽  
Eva Lindström
Keyword(s):  
Controlled Trial ◽  
Surgical Techniques ◽  
Outlet Obstruction ◽  
Major Complication ◽  
Late Complications ◽  
Stent Insertion ◽  
Endoscopic Stenting ◽  
Total Hospital Stay ◽  
Significant Difference ◽  
Cast Doubt

The development of non-surgical techniques for the relief of malignant low bileduct obstruction has cast doubt on the best way of relieving jaundice, particularly in patients fit for surgery whose life expectancy is more than a few weeks.We did a randomised prospective controlled trial comparing endoscopic stent insertion and surgical biliary bypass in patients with malignant low bileduct obstruction. 204 patients were randomised (surgery 103, stent 101); 3 subsequently proved to have benign disease and were excluded, leaving 101 surgical and 100 stented patients for assessment. Technical success was achieved in 94 surgical and 95 stent patients, with functional biliary decompression obtained in 92 patients in both groups. In stented patients, there was a lower procedure-related mortality (3% vs 14%, p=0.01), major complication rate (11% vs 29%, p=0.02), and median total hospital stay (20 vs 26 days, p=0.001). Recurrent jaundice occurred in 36 stented patients and 2 surgical patients. Late gastric outlet obstruction occurred in 17% of stented patients and 7% of the surgical group. Despite the early benefits of stenting there was no significant difference in overall survival between the two groups (median survival: surgical 26 weeks; stented 21 weeks; p=0.065).Endoscopic stenting and surgery are effective palliative treatments with the former having fewer early treatment-related complications and the latter fewer late complications.

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