scholarly journals Rapid and Simple Determination of Sarafloxacin and Difloxacin in Beef by Capillary Electrophoresis Coupled with Solid-Phase Extraction

2018 ◽  
Vol 2018 ◽  
pp. 1-9
Author(s):  
Qian Wang ◽  
Rui Zhao ◽  
Yan Cui ◽  
Cuicui Liu ◽  
Liya Liang ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Solid Phase ◽  
Background Electrolyte ◽  
Array Detector ◽  
Buffer System ◽  
Column Temperature ◽  
Experimental Parameters ◽  
Separation Temperature ◽  
Electrophoresis Method

A simple and rapid capillary electrophoresis method with diode array detector was developed for determination of sarafloxacin and difloxacin in beef. In this study, the experimental parameters affecting the determination were systematically optimized, including wavelength, buffer system, pH and concentration, and separation temperature and voltage. Under the optimal conditions, sarafloxacin and difloxacin could be quantified within 4 min using H3BO3/Na2B4O7 buffer (35 mmol/L, pH 8.8) as background electrolyte, 25 kV as separation voltage, and 22°C as the column temperature. The linear range of the method was 1–20 μg/mL with LOD 0.8 μg/mL for sarafloxacin and 0.5–20 μg/mL with LOD 0.3 μg/mL for difloxacin. The RSDs for the peak area of 8 μg/mL sarafloxacin were 4.8% (intraday) and 7.8% (interday), respectively. The proposed method has been applied to determine the residue of sarafloxacin and difloxacin in beef samples with the satisfactory recovery.

scholarly journals Validation of a Capillary Electrophoresis Method for the Simultaneous Determination of Amlodipine Besylate and Valsartan in Pharmaceuticals and Human Plasma

2011 ◽  
Vol 94 (2) ◽  
pp. 498-502 ◽  
Author(s):  
Ahmed O Alnajjar
Keyword(s):  
Capillary Electrophoresis ◽  
Simultaneous Determination ◽  
Human Plasma ◽  
Array Detector ◽  
Therapeutic Drug ◽  
Column Temperature ◽  
Photodiode Array Detector ◽  
Electrophoresis Method ◽  
Photodiode Array

Abstract A rapid, simple, and sensitive capillary electrophoresis (CE) method was developed and validated for the simultaneous determination of amlodipine (AML) and valsartan (VAL) in pharmaceuticals and human plasma using a UV photodiode array detector. Electrophoretic conditions were optimized to improve separation, sensitivity, and rapidity. The optimal conditions were 25 mM phosphate buffer at pH 8.0, injection time 10.0 s, voltage 25 kV, and column temperature 25C, with detection at 214 nm. The method was found to be linear in the range of 1.035 and 1.0350 mg/L, with weighted regression 0.9999 and 0.9994, for AML and VAL, respectively. Validation of the method showed acceptable intraday and interday accuracy (85.595.3) and precision (RSD 1.644.2) in pharmaceutical formulation and human plasma analysis. The sensitivity of the method was enhanced by both optimization of the CE procedure and preconcentration performed by liquidliquid extraction. The LOD for both AML and VAL was 0.03 mg/L, which allows analysis at the level of the drugs possibly found in human plasma. Therefore, the proposed method is suitable for QC in pharmaceutical laboratories and therapeutic drug monitoring in clinical laboratories.

scholarly journals Micellar Electrokinetic Chromatographic Study of the Separation of an Aromatase Inhibitor and a Tryciclic Antidepressant in the Breast Cancer Treatment

2008 ◽  
Vol 3 ◽  
pp. ACI.S939 ◽  
Author(s):  
J. Rodríguez Flores ◽  
A.M. Contento Salcedo ◽  
L. Muñoz Fernández
Keyword(s):  
Micellar Electrokinetic Chromatography ◽  
Solid Phase ◽  
Background Electrolyte ◽  
Kinetic Studies ◽  
Breast Cancer Treatment ◽  
Chromatographic Study ◽  
Optimal Conditions ◽  
Sodium Dodecylsulphate ◽  
Experimental Parameters

Micellar electrokinetic chromatography (MEKC) was investigated for the simultaneous determination of letrozole, imipramine and their metabolites in human urine samples over a concentration range of therapeutic interest. Experimental parameters such as pH of the running electrolyte, sodium dodecylsulphate (SDS) concentration, borate concentration, voltage, etc were investigated. Under optimal conditions of 25 mM SDS, 15 mM borate buffer (pH 9.2), 15% 2-propanol, as background electrolyte; 28 kV and 40 °C, as voltage and cartridge temperature, respectively; resolution between the peaks was greater than 1.7. Before the determination, a solid phase extraction (SPE) procedure with a C18 cartridge was optimized. Good linearity, accuracy, precision, robustness and ruggedness were achieved and detection limits of 12.5 ng/mL for letrozole and its metabolite and 37.5 ng/mL, were obtained for imipramine and their metabolites. Real determinations of these analytes in two patient urines were carried out. Sensitivity achieved in this method is sufficient to perform kinetic studies in humans.

scholarly journals Optimisation of analytical conditions for simultaneous determination of phenol and chlorophenols by capillary electrophoresis with indirect laser-induced fluorescence detection

2021 ◽  
Vol 63 (11) ◽  
pp. 51-55
Author(s):  
Manh Huy Nguyen ◽  
◽  
Hong Anh Duong ◽  
Hung Viet Pham ◽  
◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Phenolic Compounds ◽  
Simultaneous Determination ◽  
Fluorescence Detection ◽  
Background Electrolyte ◽  
Laser Induced Fluorescence ◽  
Laser Induced Fluorescence Detection ◽  
Electrophoresis Method ◽  
Wastewater Samples

This work presented a capillary electrophoresis method with indirect laser-induced fluorescence detection for the simultaneous determination of phenol and four chlorophenol derivatives. The separation was obtained within 20 minutes with a background electrolyte composed of 5 mM borax and 1 mM fluorescein (pH=9.75), 17 kV of applied voltage, and 120 s of hydrostatic injection. At the optimal conditions, the limit of detections for phenolic compounds was in the range of 0.08-0.23 mg/l, and the RSD data of repeatability and reproducibility were less than 8.0% for both migration times and peak areas. This developed method was applied to analyse concentrations of phenolic compounds in surface water and wastewater samples, with the recoveries ranging from 59.4 to 102.5%.

Determination of six anti-Parkinson drugs using cyclodextrin-capillary electrophoresis method: application to pharmaceutical dosage forms

RSC Advances ◽  
2016 ◽  
Vol 6 (21) ◽  
pp. 17519-17530 ◽  
Author(s):  
Abdallah M. Zeid ◽  
Jenny Jeehan M. Nasr ◽  
Fathalla F. Belal ◽  
Shinya Kitagawa ◽  
Noritada Kaji ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Separation Efficiency ◽  
Background Electrolyte ◽  
Dosage Forms ◽  
Inclusion Complex Formation ◽  
Pharmaceutical Dosage Forms ◽  
Capillary Electrophoresis Method ◽  
Electrophoresis Method ◽  
P Addition

Addition of β-cyclodextrin to the background electrolyte improves the separation efficiency of multi-component mixtures through inclusion complex formation.

Validated Stability-Indicating Capillary Electrophoresis Method for the Separation and Determination of a Fixed-Dose Combination of Carvedilol and Hydrochlorothiazide in Tablets

2013 ◽  
Vol 96 (5) ◽  
pp. 951-959 ◽  
Author(s):  
Nourah Z Alzoman ◽  
Maha A Sultan ◽  
Hadir M Maher ◽  
Mona M AlShehri ◽  
Ileana V Olah
Keyword(s):  
Capillary Electrophoresis ◽  
Degradation Products ◽  
Background Electrolyte ◽  
Internal Standard ◽  
Fused Silica ◽  
Fixed Dose ◽  
Array Detector ◽  
Forced Degradation ◽  
Stability Indicating

Abstract A novel, fast, sensitive, and specific capillary electrophoresis (CE) technique coupled to a diode array detector has been developed for the separation and simultaneous determination of carvedilol (CRV) and hydrochlorothiazide (HCT) in two combination formulations. The proposed method utilized a fused silica capillary (55 cm × 75 μm id) and the background electrolyte solution phosphate buffer (12.5 mM, pH 7.4)–methanol (95 + 5, v/v). The separation was achieved at 30 kV applied voltage and 24°C. Atorvastatin (80 μg/mL) was chosen as the internal standard. The described method was linear over the range of 1–200 and 0.2–150 μg/mL for CRV and HCT, respectively. Intraday and interday RSD (n = 6) was ≤1.4%. The LOD values of CRV and HCT were 0.26 and 0.07 μg/mL, respectively. The validated CE method was successfully applied to the analysis of two commercial tablet dosage forms. Forced degradation studies were performed on bulk samples of the two drugs using thermal, photolytic, hydrolytic, and oxidative stress conditions, and the stressed samples were analyzed by the proposed method. Degradation products produced as a result of stress studies did not interfere with the determination of CRV and HCT; the assay could, therefore, be considered stability-indicating.

scholarly journals Determination of Maleic Hydrazide in Pesticide Formulations by Capillary Electrophoresis

1998 ◽  
Vol 81 (6) ◽  
pp. 1109-1112 ◽  
Author(s):  
Donna T Kubilius ◽  
Rodney J Bushway
Keyword(s):  
Capillary Electrophoresis ◽  
Maleic Hydrazide ◽  
Array Detector ◽  
Photodiode Array Detector ◽  
Coefficients Of Variation ◽  
Pesticide Formulations ◽  
Electrophoresis Method ◽  
Photodiode Array ◽  
Total Analysis

Abstract A capillary electrophoresis method was developed to quantitate the growth regulator maleic hydrazide (MH) in pesticide formulations. Liquid formulations were dissolved in water, injected into a capillary electrophoresis instrument, and monitored at 220 nm. The running buffer was 10 mM dibasic sodium phosphate at pH 9.0. Total analysis time was 10 min. Four liquid formulations with a guarantee of 30.2 or 30.3% potassium salt of MH were analyzed. Within-day coefficients of variation (CVs) ranged from 3.0 to 7.7%, and between-day CVs ranged from 5.5 to 10.0%. Purity of each MH peak was checked by using a photodiode array detector in the spectrum mode. No interferences were observed.

scholarly journals Study on development of an analytical procedure to quantify glyphosate by capillary electrophoresis method and its application for beverage

2021 ◽  
Vol 63 (11) ◽  
pp. 58-64
Author(s):  
Manh Huy Nguyen ◽  
◽  
Thi Ha Tran ◽  
Minh Tuan Vu ◽  
Thanh Dam Nguyen ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Capillary Electrophoresis ◽  
Analytical Procedure ◽  
Ph Value ◽  
Solid Phase ◽  
Residue Analysis ◽  
Background Electrolyte ◽  
Capillary Electrophoresis Method ◽  
Relative Standard ◽  
Electrophoresis Method

Nowadays, the issue of fresh food, drinks, and residue analysis of toxic compounds in food and drinks is increasingly interested in society. In this study, an analytical procedure for quantitative analysis of the glyphosate residue in beverages such as tea infusion and beer was developed based on the capillary electrophoresis method with capacitively-coupled contactless conductivity detector combined with sample preparation using solid phase extraction technique. The analytical conditions optimised for capillary electrophoresis equipment included: histidine/acetic acid background electrolyte solution with histidine concentration of 1 mM and pH value was adjusted to 2.75 by acetic acid; a separation voltage was 20 kV was applied and a high voltage injection at 20 kV for 10 seconds was chosen. The optimised analytical procedure has resulted in a low detection limit of glyphosate (0.42 μg/l), the repeatability and reproducibility expressed by the relative standard deviation of the peak area and migration time were both less than 10%, a wide linear range, and the obtained recovery efficiency of glyphosate on different drinks were achieved to values ranging from 88.7 to 96.3%.

Determination of Niacin in Infant Formula by Solid-phase Clean-up and HPLC with Photodiode Array Detector

2009 ◽  
Vol 38 (3) ◽  
pp. 359-363
Author(s):  
Jee-Eun Hong ◽  
Mi-Ran Kim ◽  
Sang-Hee Cheon ◽  
Jung-Young Chai ◽  
Eun-Ryong Park ◽  
...  
Keyword(s):  
Infant Formula ◽  
Solid Phase ◽  
Array Detector ◽  
Photodiode Array Detector ◽  
Photodiode Array

Ultrasound-assisted magnetic solid-phase extraction for the determination of some transition metals in Orujo spirit samples by capillary electrophoresis

2016 ◽  
Vol 190 ◽  
pp. 263-269 ◽  
Author(s):  
Rosa M. Peña Crecente ◽  
Carlha Gutiérrez Lovera ◽  
Julia Barciela García ◽  
Carlos Herrero Latorre ◽  
Sagrario García Martín
Keyword(s):  
Capillary Electrophoresis ◽  
Transition Metals ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Magnetic Solid Phase Extraction ◽  
Phase Extraction ◽  
Magnetic Solid ◽  
Ultrasound Assisted
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