scholarly journals Recent Progress of Fabrication of Cell Scaffold by Electrospinning Technique for Articular Cartilage Tissue Engineering

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Yingge Zhou ◽  
Joanna Chyu ◽  
Mimi Zumwalt
Keyword(s):  
Tissue Engineering ◽  
3D Structure ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Natural Polymers ◽  
Tissue Engineering Scaffolds ◽  
Electrospinning Technique ◽  
3D Scaffolds ◽  
Cell Growth And Differentiation ◽  
Material Transportation

As a versatile nanofiber manufacturing technique, electrospinning has been widely employed for the fabrication of tissue engineering scaffolds. Since the structure of natural extracellular matrices varies substantially in different tissues, there has been growing awareness of the fact that the hierarchical 3D structure of scaffolds may affect intercellular interactions, material transportation, fluid flow, environmental stimulation, and so forth. Physical blending of the synthetic and natural polymers to form composite materials better mimics the composition and mechanical properties of natural tissues. Scaffolds with element gradient, such as growth factor gradient, have demonstrated good potentials to promote heterogeneous cell growth and differentiation. Compared to 2D scaffolds with limited thicknesses, 3D scaffolds have superior cell differentiation and development rate. The objective of this review paper is to review and discuss the recent trends of electrospinning strategies for cartilage tissue engineering, particularly the biomimetic, gradient, and 3D scaffolds, along with future prospects of potential clinical applications.

scholarly journals Electrospun Cellulose-Silk Composite Nanofibres Direct Mesenchymal Stem Cell Chondrogenesis in the Absence of Biological Stimulation

2018 ◽  
Author(s):  
Runa Begum ◽  
Adam W. Perriman ◽  
Bo Su ◽  
Fabrizio Scarpa ◽  
Wael Kafienah
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Growth Factor ◽  
Polymer Composite ◽  
Cartilage Tissue Engineering ◽  
Stem Cell Differentiation ◽  
Cartilage Tissue ◽  
Natural Polymer ◽  
Natural Polymers ◽  
Electrospinning Technique

AbstractSmart biomaterials with an inherent stimulating capacity that elicit specific behavioursin lieuof biological prompts would prove advantageous for regenerative medicine applications. Specific blends of the natural polymers cellulose and silk cast as films can drive the chondrogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs) uponin vitroculture. However, the true potential of such biomaterials for cartilage tissue engineering can be realised upon its three-dimensional fabrication. In this work we employ an electrospinning technique to model thein vivonanofibrous extracellular matrix (ECM). Cellulose and silk polymers at a mass ratio of 75:25 were regenerated using a trifluoroacetic acid and acetic acid cosolvent system. This natural polymer composite was directly electrospun for the first time, into nanofibers without post-spun treatment. The presence and size of fibre beading was influenced by environmental humidity. The regenerated composite retained the key chemical functionalities of its respective components. Biocompatibility of the natural polymer composite with hMSCs was demonstrated and its inherent capacity to direct chondrogenic stem cell differentiation, in the absence of stimulating growth factors, was confirmed. This physical chondrogenic stimulation was countered biochemically using fibroblast growth factor-2 (FGF-2), a growth factor used to enhance the proliferation of hMSCs. The newly fabricated scaffold provides the foundation for designing a robust, self-inductive, and cost-effective biomimetic biomaterial for cartilage tissue engineering.

scholarly journals Acrylic Acid Plasma Coated 3D Scaffolds for Cartilage tissue engineering applications

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Pieter Cools ◽  
Carlos Mota ◽  
Ivan Lorenzo-Moldero ◽  
Rouba Ghobeira ◽  
Nathalie De Geyter ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Acrylic Acid ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Engineering Applications ◽  
3D Scaffolds

New strategies for cartilage regeneration exploiting selected glycosaminoglycans to enhance cell fate determination

2014 ◽  
Vol 42 (3) ◽  
pp. 703-709 ◽  
Author(s):  
Bethanie I. Ayerst ◽  
Anthony J. Day ◽  
Victor Nurcombe ◽  
Simon M. Cool ◽  
Catherine L.R. Merry
Keyword(s):  
Tissue Engineering ◽  
Cell Fate ◽  
Cartilage Tissue Engineering ◽  
Cartilage Regeneration ◽  
Stem Cell Differentiation ◽  
Biologically Active ◽  
Cartilage Tissue ◽  
Research Strategies ◽  
Tissue Engineering Scaffolds ◽  
Ecm Extracellular Matrix

Most research strategies for cartilage tissue engineering use extended culture with complex media loaded with costly GFs (growth factors) to drive tissue assembly and yet they result in the production of cartilage with inferior mechanical and structural properties compared with the natural tissue. Recent evidence suggests that GAGs (glycosaminoglycans) incorporated into tissue engineering scaffolds can sequester and/or activate GFs and thereby more effectively mimic the natural ECM (extracellular matrix). Such approaches may have potential for the improvement of cartilage engineering. However, natural GAGs are structurally complex and heterogeneous, making structure–function relationships hard to determine and clinical translation difficult. Importantly, subfractions of GAGs with specific chain lengths and sulfation patterns have been shown to activate key signalling processes during stem cell differentiation. In addition, recently, GAGs have been bound to synthetic biomaterials, such as electrospun scaffolds and hydrogels, in biologically active conformations, and methods to purify and select affinity-matched GAGs for specific GFs have also been developed. The identification and use of specific GAG moieties to promote chondrogenesis is therefore an exciting new avenue of research. Combining these with synthetic biomaterials may allow a more effective mimicry of the natural ECM, reduction in the need for expensive GFs, and perhaps the deposition of an articular cartilage-like matrix in a clinically relevant manner.

scholarly journals Design and Dynamic Culture of 3D-Scaffolds for Cartilage Tissue Engineering

2009 ◽  
Vol 25 (5) ◽  
pp. 429-444 ◽  
Author(s):  
Rouwayda El-Ayoubi ◽  
Christian DeGrandpré ◽  
Robert DiRaddo ◽  
Azizeh-Mitra Yousefi ◽  
Patrick Lavigne
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
3D Scaffolds ◽  
Dynamic Culture

A Chondroitin Sulfate based Injectable Hydrogel for Delivery of Stem Cells in Cartilage Regeneration

2021 ◽  
Author(s):  
Xiaolin Li ◽  
Qian Xu ◽  
Melissa Johnson ◽  
Xi Wang ◽  
Jing Lyu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Chondroitin Sulfate ◽  
Cartilage Tissue Engineering ◽  
Cartilage Regeneration ◽  
Cartilage Tissue ◽  
Tissue Engineering Scaffolds ◽  
Injectable Hydrogel ◽  
Rapid Degradation

Chondroitin sulfate (CS), as a popular material for cartilage tissue engineering scaffolds, has been extensively studied and reported for its safety and excellent biocompatibility. However, the rapid degradation of pure...

scholarly journals Supermacroprous chitosan–agarose–gelatin cryogels: in vitro characterization and in vivo assessment for cartilage tissue engineering

2010 ◽  
Vol 8 (57) ◽  
pp. 540-554 ◽  
Author(s):  
Sumrita Bhat ◽  
Anuj Tripathi ◽  
Ashok Kumar
Keyword(s):  
Tissue Engineering ◽  
Fluorescent Microscopy ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Laboratory Animals ◽  
Natural Polymers ◽  
Unconfined Compression Test ◽  
Polymeric Scaffold

The study focuses on the synthesis of a novel polymeric scaffold having good porosity and mechanical characteristics synthesized by using natural polymers and their optimization for application in cartilage tissue engineering. The scaffolds were synthesized via cryogelation technology using an optimized ratio of the polymer solutions (chitosan, agarose and gelatin) and cross-linker followed by the incubation at sub-zero temperature (−12°C). Microstructure examination of the chitosan–agarose–gelatine (CAG) cryogels was done using scanning electron microscopy (SEM) and fluorescent microscopy. Mechanical analysis, such as the unconfined compression test, demonstrated that cryogels with varying chitosan concentrations, i.e. 0.5–1% have a high compression modulus. In addition, fatigue tests revealed that scaffolds are suitable for bioreactor studies where gels are subjected to continuous cyclic strain. In order to confirm the stability, cryogels were subjected to high frequency (5 Hz) with 30 per cent compression of their original length up to 1 × 10 5 cycles, gels did not show any significant changes in their mass and dimensions during the experiment. These cryogels have exhibited degradation capacity under aseptic conditions. CAG cryogels showed good cell adhesion of primary goat chondrocytes examined by SEM. Cytotoxicity of the material was checked by MTT assay and results confirmed the biocompatibility of the material. In vivo biocompatibility of the scaffolds was checked by the implantation of the scaffolds in laboratory animals. These results suggest the potential of CAG cryogels as a good three-dimensional scaffold for cartilage tissue engineering.

scholarly journals Review of Synthetic and Hybrid Scaffolds in Cartilage Tissue Engineering

Membranes ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 348
Author(s):  
Monika Wasyłeczko ◽  
Wioleta Sikorska ◽  
Andrzej Chwojnowski
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Hybrid Materials ◽  
Cartilage Tissue Engineering ◽  
Biological Properties ◽  
General Information ◽  
Cartilage Tissue ◽  
Cartilage Defects ◽  
Natural Polymers ◽  
Cellular Environment

Cartilage tissue is under extensive investigation in tissue engineering and regenerative medicine studies because of its limited regenerative potential. Currently, many scaffolds are undergoing scientific and clinical research. A key for appropriate scaffolding is the assurance of a temporary cellular environment that allows the cells to function as in native tissue. These scaffolds should meet the relevant requirements, including appropriate architecture and physicochemical and biological properties. This is necessary for proper cell growth, which is associated with the adequate regeneration of cartilage. This paper presents a review of the development of scaffolds from synthetic polymers and hybrid materials employed for the engineering of cartilage tissue and regenerative medicine. Initially, general information on articular cartilage and an overview of the clinical strategies for the treatment of cartilage defects are presented. Then, the requirements for scaffolds in regenerative medicine, materials intended for membranes, and methods for obtaining them are briefly described. We also describe the hybrid materials that combine the advantages of both synthetic and natural polymers, which provide better properties for the scaffold. The last part of the article is focused on scaffolds in cartilage tissue engineering that have been confirmed by undergoing preclinical and clinical tests.

scholarly journals Supercritical carbon dioxide: putting the fizz into biomaterials

2005 ◽  
Vol 364 (1838) ◽  
pp. 249-261 ◽  
Author(s):  
John J.A Barry ◽  
Marta M.C.G Silva ◽  
Vladimir K Popov ◽  
Kevin M Shakesheff ◽  
Steven M Howdle
Keyword(s):  
Tissue Engineering ◽  
Supercritical Fluids ◽  
Recent Progress ◽  
Cartilage Tissue Engineering ◽  
Three Dimensional ◽  
Cartilage Tissue ◽  
Sintering Process ◽  
Tissue Engineering Scaffolds ◽  
Biodegradable Polyesters ◽  
Made In

This paper describes recent progress made in the use of high pressure or supercritical fluids to process polymers into three-dimensional tissue engineering scaffolds. Three current examples are highlighted: foaming of acrylates for use in cartilage tissue engineering; plasticization and encapsulation of bioactive species into biodegradable polyesters for bone tissue engineering; and a novel laser sintering process used to fabricate three-dimensional biodegradable polyester structures from particles prepared via a supercritical route.

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