scholarly journals Protective Effects of Benzoic Acid,BacillusCoagulans, and Oregano Oil on Intestinal Injury Caused by EnterotoxigenicEscherichia coliin Weaned Piglets

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Junning Pu ◽  
Daiwen Chen ◽  
Gang Tian ◽  
Jun He ◽  
Ping Zheng ◽  
...  
Keyword(s):  
Benzoic Acid ◽  
Growth Performance ◽  
Intestinal Barrier ◽  
Mucosal Barrier ◽  
Jejunal Mucosa ◽  
Protective Effects ◽  
Growth Promoters ◽  
Combined Supplementation ◽  
Oregano Oil ◽  
Siga Level

The use of antibiotics as growth promoters in feed has been fully or partially banned in several countries. The objective of this study was to investigate the effects of benzoic acid (A),bacilluscoagulans (B) and oregano oil (O) combined supplementation on growth performance and intestinal barrier in piglets challenged with enterotoxigenicEscherichia coli(ETEC). Thirty piglets were randomly assigned to 6 treatments: (1) nonchallenged control (CON); (2) ETEC-challenged control (ETEC); (3) antibiotics + ETEC (AT); (4) A + B + ETEC (AB); (5) A + O + ETEC (AO); (6) A + B + O + ETEC (ABO). On day 22, piglets were orally challenged with ETEC or saline. The trial lasted 26 days. Dietary AO and ABO inhibited the reduction of growth performance and the elevation of diarrhoea incidence in piglets induced by ETEC (P<0.05). AB, AO, and ABO prevented the elevation of serum TNF-αand LPS concentrations in piglets induced by ETEC (P<0.05). ABO alleviated the elevation of TNF-αand IL-1βconcentrations and the reduction of sIgA level in jejunal mucosa induced by ETEC (P<0.05). Furthermore, ABO upregulated mRNA expressions of Claudin-1 and Mucin2 (P<0.05), downregulated mRNA abundances of TLR4 and NOD2 signaling pathways related genes in jejunal mucosa (P<0.05), and improved the microbiota in jejunal and cecal digesta (P<0.05) compared with ETEC group. These results indicated that benzoic acid,bacilluscoagulans, and oregano oil combined supplementation could improve growth performance and alleviate diarrhoea of piglets challenged with ETEC via improving intestinal mucosal barrier integrity, which was possibly associated with the improvement of intestinal microbiota and immune status. The combination of 3000 g/t benzoic acid + 400 g/tbacilluscoagulans + 400 g/t oregano oil showed better effects than other treatments in improving growth performance and intestinal health of piglets, which could be used as a viable substitute for antibiotic.

scholarly journals Supplemental Bacillus subtilis DSM 29784 and enzymes, alone or in combination, as alternatives for antibiotics to improve growth performance, digestive enzyme activity, anti-oxidative status, immune response and the intestinal barrier of broiler chickens

2020 ◽  
pp. 1-14 ◽  
Author(s):  
Yuanyuan Wang ◽  
Chianning Heng ◽  
Xihong Zhou ◽  
Guangtian Cao ◽  
Lei Jiang ◽  
...  
Keyword(s):  
Immune Response ◽  
Enzyme Activity ◽  
Bacillus Subtilis ◽  
Growth Performance ◽  
Intestinal Microbiota ◽  
Broiler Chickens ◽  
Digestive Enzyme ◽  
Intestinal Barrier ◽  
Digestive Enzyme Activity ◽  
Jejunal Mucosa

Abstract The present study investigated the effect of Bacillus subtilis DSM 29784 (Ba) and enzymes (xylanase and β-glucanases; Enz), alone or in combination (BE) as antibiotic replacements, on the growth performance, digestive enzyme activity, immune response and the intestinal barrier of broiler chickens. In total, 1200 1-d-old broilers were randomly assigned to five dietary treatments, each with six replicate pens of forty birds for 63 d as follows: (a) basal diet (control), supplemented with (b) 1 × 109 colony-forming units (cfu)/kg Ba, (c) 300 mg/kg Enz, (d) 1 × 109 cfu/kg Ba and 300 mg/kg Enz and (e) 250 mg/kg enramycin (ER). Ba, Enz and BE, similar to ER, decreased the feed conversion rate, maintained intestinal integrity with a higher villus height:crypt depth ratio and increased the numbers of goblet cells. The BE group exhibited higher expression of claudin-1 and mucin 2 than the other four groups. BE supplementation significantly increased the α-diversity and β-diversity of the intestinal microbiota and markedly enhanced lipase activity in the duodenal mucosa. Serum endotoxin was significantly decreased in the BE group. Compared with those in the control group, increased superoxide dismutase and glutathione peroxidase activities were observed in the jejunal mucosa of the Ba and BE groups, respectively. In conclusion, the results suggested that dietary treatment with Ba, Enz or BE has beneficial effects on growth performance and anti-oxidative capacity, and BE had better effects than Ba or Enz alone on digestive enzyme activity and the intestinal microbiota. Ba or Enz could be used as an alternative to antibiotics for broiler chickens.

scholarly journals Effects of Chronic Exposure to Low Levels of Dietary Aflatoxin B1 on Growth Performance, Apparent Total Tract Digestibility and Intestinal Health in Pigs

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 336
Author(s):  
Junning Pu ◽  
Qinghui Yuan ◽  
Hui Yan ◽  
Gang Tian ◽  
Daiwen Chen ◽  
...  
Keyword(s):  
Growth Performance ◽  
Aflatoxin B1 ◽  
Chronic Exposure ◽  
Transforming Growth Factor ◽  
Intestinal Barrier ◽  
Basal Diet ◽  
Mrna Abundance ◽  
Jejunal Mucosa ◽  
Intestinal Health ◽  
Low Levels

This study aimed to investigate the effects of chronic exposure to low levels of dietary aflatoxin B1 (AFB1) on growth performance, apparent total tract digestibility and intestinal health in pigs. In a 102-day experiment, fourteen barrows (Duroc×Landrace×Yorkshire, initial BW = 38.21 ± 0.45 kg) were randomly divided into control (CON, basal diet) and AFB1 groups (the basal diet supplemented with 280 μg/kg AFB1). Results revealed that the AFB1 exposure decreased the final BW, ADFI and ADG in pigs (p < 0.10). AFB1 exposure also decreased the apparent total tract digestibility of dry mater and gross energy at 50 to 75 kg and 105 to 135 kg stages, and decreased the apparent total tract digestibility of ether extract at 75 to 105 kg stage (p < 0.05). Meanwhile, AFB1 exposure increased serum diamine oxidase activity and reduced the mRNA abundance of sodium-glucose cotransporter 1, solute carrier family 7 member 1 and zonula occluden-1 in the jejunal mucosa (p < 0.05). Furthermore, AFB1 exposure decreased superoxide dismutase activity (p < 0.05) and increased 8-hydroxy-2′-deoxyguanosine content (p < 0.10) in jejunal mucosa. AFB1 exposure also increased tumor necrosis factor-α, interleukin-1β and transforming growth factor-β mRNA abundance in jejunal mucosa and upregulated Escherichia coli population in colon (p < 0.05). The data indicated that chronic exposure to low levels of dietary AFB1 suppressed growth performance, reduced the apparent total tract digestibility and damaged intestinal barrier integrity in pigs, which could be associated with the decreased intestinal antioxidant capacity and the increased pro-inflammatory cytokine production.

scholarly journals Chloroquine Improves Deoxynivalenol-Induced Inflammatory Response and Intestinal Mucosal Damage in Piglets

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Simeng Liao ◽  
Shengguo Tang ◽  
Bie Tan ◽  
Jianjun Li ◽  
Ming Qi ◽  
...  
Keyword(s):  
Growth Performance ◽  
Inflammatory Responses ◽  
Control Volume ◽  
Average Daily Gain ◽  
Intestinal Barrier ◽  
Intestinal Morphology ◽  
Mucosal Barrier ◽  
Control Group ◽  
Junction Protein

We investigated the effects of rapamycin (RAPA) and chloroquine (CQ) in supporting growth performance and the intestinal mucosal barrier in response to deoxynivalenol (DON) in piglets. A total of 32 healthy weaned piglets (bodyweight 7.10±0.58 kg) were divided into four groups and treated daily with RAPA (1 mg/kg BW), CQ (10 mg/kg BW), or a control volume of normal saline (two groups) until the end of the experiment. After feeding a basal diet for seven days, three groups were then switched to mildewed feed containing 1 mg kg/DON for a further seven days. In contrast to the control group, DON-treated piglets showed decreased average daily gain (ADG) and daily feed intake (ADFI), as well as negatively affected intestinal morphology as indicated by villus height, crypt depth, and tight junction protein expression. A group treated with RAPA and DON showed increased intestinal autophagy, aggravated inflammatory responses, and damage to the intestinal mucosa and permeability, leading to reduced growth performance. Meanwhile, a group treated with CQ and DON showed indices comparable to the non-DON control group, with alleviated inflammatory cytokines and healthy intestinal morphology and structure. They also showed better growth performance compared to DON treatment alone. These findings have important implications for mediating autophagy against DON in vivo, as well as the potential for CQ in improving growth performance and maintaining intestinal barrier integrity in weanling piglets.

scholarly journals Improvement of growth performance and parameters of intestinal function in liquid fed early weanling pigs1

2019 ◽  
Vol 97 (7) ◽  
pp. 2725-2738 ◽  
Author(s):  
Junjie Jiang ◽  
Daiwen Chen ◽  
Bing Yu ◽  
Jun He ◽  
Jie Yu ◽  
...  
Keyword(s):  
Growth Performance ◽  
Control Diet ◽  
Intestinal Barrier ◽  
Basal Diet ◽  
Nutrient Digestibility ◽  
Jejunal Mucosa ◽  
Weanling Pigs ◽  
Barrier Functions ◽  
Liquid Feeding ◽  
Zonula Occludens

Abstract Liquid feeding, a widely used technique, has been applied as a feeding technique commonly in global swine production. The objective of this study was to evaluate the effects of liquid feeding on growth performance, nutrient digestibility, and intestinal barrier functions during the early weaning period in pigs. Three hundred and sixty 24-d-old weanling pigs (Duroc × Landrace × Yorkshire) with BW of 6.98 ± 0.15 kg were randomly assigned to a control diet (dry fed basal diet, CON) or as meal mixed with water in the ratio 1:4 (liquid fed basal diet, LF) with 6 replicates per treatment and 30 weanling pigs per replicate. The study lasted 7 d. On days 4 to 7, fresh fecal samples were collected to evaluate apparent total tract digestibility (ATTD) of nutrients. After 7 d, 2 weanling pigs per pen were euthanized and physiological samples were obtained. Results showed that LF increased (P < 0.05) ADG (281 g vs. 183 g), ADFI (374 g vs. 245 g), and final BW (8.95 kg vs. 8.26 kg) compared with CON. Compared with CON, LF significantly decreased (P < 0.05) serum cortisol and d-lactate concentrations as well as the activity of diamine oxidase, enhanced (P < 0.05) the ATTD of ether extract and ash, increased (P < 0.05) the activities of amylase, lipase, and lactase in the jejunal mucosa. Furthermore, LF had higher (P < 0.05) villus height and villi height:crypt depth and increased (P < 0.05) mRNA expressions of insulin-like growth factors-1 receptor (IGF-1R), claudin-2 (CLDN-2), zonula occludens-1 (ZO-1), and zonula occludens-2 (ZO-2) in the jejunum. Moreover, LF had lower (P < 0.05) abundances of total bacteria and Escherichia coli and higher (P < 0.05) concentrations of acetic acid and butyric acid in cecal digesta. Altogether, the results indicated that liquid feeding not only promoted growth performance but also improved intestinal health by enhancing gut barrier functions in weanling pigs.

scholarly journals Purified β-glucans of Different Molecular Weights Enhance Growth Performance of LPS-challenged Piglets via Improved Gut Barrier Function and Microbiota

Animals ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 602
Author(s):  
Junqiu Luo ◽  
Daiwen Chen ◽  
Xiangbing Mao ◽  
Jun He ◽  
Bing Yu ◽  
...  
Keyword(s):  
Growth Performance ◽  
Barrier Function ◽  
Volatile Fatty Acids ◽  
Body Weight Loss ◽  
Mucosal Barrier ◽  
Jejunal Mucosa ◽  
Molecular Weights ◽  
Gut Barrier Function ◽  
Mucosal Barrier Function ◽  
Gut Function

This study investigated β-glucan derived from Agrobacterium sp. ZX09 with high (2000 kDa) and low (300 kDa) molecular weight (MW) to compare their effects on growth performance and gut function in LPS-induced weaned piglets. Changes in jejunal morphology, mucosal barrier function, microbial populations, and fermentation in the piglets were determined. Data showed that β-glucan prevented body weight loss in LPS challenged piglets. Supplementation with both β-glucan fractions improved jejunal morphology. Compared to low MW, β-glucan of high MW generally up-regulated transcripts of ZO-1, MUC1, and MUC2 in jejunal mucosa to a lesser extent. Mucosal D-lactate, diamine oxidase, and anti-oxidation index were effectively resumed in β-glucan treatment. Both β-glucan diets provoked the emergence of a balanced microbiota and a richer concentration of volatile fatty acids in the colon. The richest community of bifidobacterium and concentration of butyrate emerged after feeding β-glucan with high MW. Results suggested that the effect of Agrobacterium sp. ZX09 β-glucans on the gut-modulatory function is largely linked to their MW. Low MW β-glucan mainly improved the mucosal barrier function in the jejunum, while high MW β-glucan had profound effects on the microbial community and fermentation in the hindgut of piglets.

A critical role for nitric oxide in intestinal barrier function and dysfunction

1996 ◽  
Vol 270 (2) ◽  
pp. G225-G237 ◽  
Author(s):  
I. Alican ◽  
P. Kubes
Keyword(s):  
Nitric Oxide ◽  
Leukocyte Adhesion ◽  
Critical Role ◽  
Intestinal Barrier ◽  
Mucosal Barrier ◽  
Intestinal Barrier Function ◽  
Reactive Oxygen Metabolites ◽  
Protective Effects ◽  
Mucosal Permeability ◽  
Cell Reactivity

There is growing evidence that endogenous nitric oxide (NO) regulates mucosal barrier integrity under physiological conditions and counters the increase in mucosal permeability associated with acute pathophysiological states. The potential mechanisms of action for the protective effects of NO are discussed. These include maintenance of blood flow, inhibition of platelet and leukocyte adhesion and/or aggregation within the vasculature, modulation of mast cell reactivity, and scavenging of reactive oxygen metabolites such as superoxide. On the basis of the data presented, we conclude that both constitutive nitric oxide synthase (cNOS)-derived endogenous NO and exogenous NO (from NO donors) appear to reduce the sequelae of acute inflammation. The second section of this review summarizes the data germane to prolonged (chronic) inflammatory conditions associated with the overproduction of NO from the inducible form of NOS (iNOS). Some emphasis is placed on the role of NO in sepsis and inflammatory bowel disease (IBD), and data to suggest that NO, or more specifically a NO-derived mediator, is involved in these disorders are summarized. These studies are compared with recent publications suggesting that inhibition of NO synthesis with nonspecific inhibitors of NOS or selective iNOS inhibitors may not protect in models of sepsis or IBD. Overall, the review highlights the potential importance of the type of NOS enzyme involved in the particular inflammatory process being studied.

Effects of Montmorillonite on Growth Performance, Serum Biochemistry and Oxidative Stress of Red-Crowned Crane (Grus japonensis) Fed Mycotoxin-Contaminated Feed

2020 ◽  
Vol 21 (8) ◽  
pp. 626-632 ◽  
Author(s):  
Dawei Liu ◽  
Qinghua Wu ◽  
Hongyi Liu ◽  
Changhu Lu ◽  
Chao Gu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Growth Performance ◽  
Feed Intake ◽  
Animal Feed ◽  
Bird Species ◽  
Serum Biochemistry ◽  
Protective Effects ◽  
Regular Diet ◽  
Grus Japonensis ◽  
And Oxidative Stress

Background: The red-crowned crane (Grus japonensis) is one of the most vulnerable bird species in the world. Mycotoxins are toxic secondary metabolites produced by fungi and considered naturally unavoidable contaminants in animal feed. Our recent survey indicated that the mycotoxins had the potential to contaminate redcrowned crane’s regular diets in China. Objective: This experiment was conducted to investigate the protective effects of mycotoxin binder montmorillonite (Mont) on growth performance, serum biochemistry and oxidative stress parameters of the red-crowned crane. Methods: 16 red-crowned cranes were divided into four groups and fed one of the following diets; a selected diet, regular diet, or the selected diet or regular diet with 0.5% montmorillonite added to the diets. The cranes' parameters of performance, hematology, serum biochemistry and serum oxidative stress were measured. Results: Consuming regular diets decreased the average daily feed intake (ADFI), levels of haemoglobin (Hb), platelet count (PLT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), but increased the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine kinase (CK) and lactate dehydrogenase (LDH). The supplementation of 0.5% Mont provided protection for the red-crowned crane in terms of feed intake, serum biochemistry and oxidative stress. Moreover, Mont supplementation had no adverse effect on the health of red-crowned crane. Conclusions: Taken together, these findings suggested that the addition of dietary Mont is effective in improving the health of red-crowned crane.

PSVI-16 The Association of Benzoic Acid and Essential Oils Eo Reduces the Inflammatory Response of Weaned Piglets and the Diarrhea Incidence in the Nursery Phase

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 202-203
Author(s):  
Sudario Roberto Silva Junior ◽  
Maíra Resende ◽  
Rhuan F Chaves ◽  
Jéssica Aparecida Barbosa ◽  
Iana I M Ferreira ◽  
...  
Keyword(s):  
Body Weight ◽  
Inflammatory Response ◽  
Benzoic Acid ◽  
Essential Oils ◽  
Growth Performance ◽  
Block Design ◽  
Basal Diet ◽  
Weaned Piglets ◽  
Total Period ◽  
Diarrhea Incidence

Abstract Benzoic acid (BA) and essential oils (EO) can minimize growth performance losses due to the removal of antibiotics and change the intestinal health of weaned piglets. The objective of this study was to evaluate the effects of BA and EO on inflammatory response, diarrhea incidence, and growth performance of the nursery phase. One hundred and twenty barrows were weaned at 23 days (6.40 ± 0.53 kg) and assigned into 3 treatments (10 replicates) in randomized block design: basal diet without additives (NC), basal diet with 200 ppm of colistin sulphate (PC), and association of 0,3% benzoic acid and essential oil (BA+EO). The feed intake and body weight were recorded at 0 and 42 days. The feces were assessed daily (per animal) and graded as normal feces (no diarrhea) or liquid or pasty stools (presence of diarrhea). On days 1, 3, and 9, blood samples were collected (5 replicates) for white blood cells (WBC) counts. Growth performance was analyzed by MIXED procedure (SAS, 2009) and the Tukey test was used to compare the means (P &lt; 0.050). The WBC counts were analyzed by repeated-measures analysis of variance, by MIXED procedure. Diarrhea incidence was analyzed by GENMOD procedure (SAS, 2009). The BA+EO treatment showed a similar body weight (P = 0.014) and average daily gain (P = 0.012) than the PC group and lower feed conversion ratio (P = 0.037) compared to the NC group. The pigs of the BA+EO treatment had the lowest diarrhea incidence during the total period (P &lt; 0.001). The supplementation with BA+EO or antibiotics reduced the counts of total WBC (P = 0.008) and neutrophils (P = 0.003). In conclusion, supplementation with BA+EO reduces the inflammatory response and the incidence of diarrhea in the nursery phase, that may be related to the improvement in the FCR.

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